BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	464,595	A→G	6.0%	K91R (AAA→AGA)	USA300HOU_0439 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	464,595	0	A	G	6.0%	286.4 / 6.6	99	K91R (AAA→AGA)	USA300HOU_0439	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (52/41); new base G (2/4); total (54/45)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 107 total aligned reads displayed.

GAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAA  >  CP000730/464455‑464734

gagCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGtaataa                                                                                                                                             <  2:464706/141‑1 (MQ=35)

 agCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGTTGTGGATGATATATACAGATTTCGCCAAAAGTAATaaa                                                                                                                                            >  1:544080/1‑141 (MQ=25)

  gCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAAc                                                                                                                                           >  1:127517/1‑141 (MQ=35)

   cttgacGAAAACAAAAGATATGAATCAAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATAAAAAAAAAACAAAAAGTAATAAAcc                                                                                                                                          <  2:195009/136‑1 (MQ=11)

    tttGAGAAAACATGAGATATGTATCCAATAAAGAATCGCGAGGACTTATATGAAAAAGAAGGATATCGAGATAGAGAAAAAAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCg                                                                                                                                         <  2:1068148/141‑1 (MQ=11)

     ttGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCgg                                                                                                                                        >  1:1027006/1‑141 (MQ=35)

      tGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCggg                                                                                                                                       >  1:689502/1‑141 (MQ=35)

          aaaaCATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTa                                                                                                                                   <  1:380957/141‑1 (MQ=35)

            aaCATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTAtt                                                                                                                                 <  2:1018312/141‑1 (MQ=35)

             aCATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTa                                                                                                                                <  2:546705/141‑1 (MQ=37)

                   gATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAAt                                                                                                                          >  1:657921/1‑141 (MQ=40)

                    atatGTATCCAATCAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGCTAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGCTTTCGCCCAAAGTAATAAACCGGGTGTATTCGATAATg                                                                                                                         >  2:583111/1‑141 (MQ=11)

                       tGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAg                                                                                                                      >  1:779996/1‑141 (MQ=40)

                        gTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAgg                                                                                                                     >  2:297427/1‑141 (MQ=40)

                            ccAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATg                                                                                                                 >  2:839507/1‑141 (MQ=255)

                            ccAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATg                                                                                                                 >  1:235158/1‑141 (MQ=40)

                              aatcaaaaaTCTCGAGGACCTATATGATAAAGAAGGAGATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATggt                                                                                                               <  2:29586/134‑3 (MQ=11)

                              aaTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAt                                                                                                               <  1:201087/141‑1 (MQ=255)

                               aTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtt                                                                                                              <  1:706865/141‑1 (MQ=255)

                               aTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtt                                                                                                              <  2:437822/141‑1 (MQ=255)

                                 tAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtttt                                                                                                            <  1:172201/141‑1 (MQ=255)

                                 tAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtttt                                                                                                            <  1:423789/141‑1 (MQ=255)

                                  aaGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTa                                                                                                           >  1:203775/1‑141 (MQ=255)

                                   aGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGCTAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTaa                                                                                                          <  1:802677/141‑1 (MQ=255)

                                      aTCTCGAGGACTTATATGACGAGGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAAtt                                                                                                       <  2:662984/141‑1 (MQ=255)

                                      aTCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAAtt                                                                                                       >  2:887942/1‑141 (MQ=255)

                                      aTCTCGAGGACCTATATGATAAAGAAGGATATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAAtt                                                                                                       >  1:63482/1‑141 (MQ=11)

                                         tcGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTgg                                                                                                    >  1:963693/1‑141 (MQ=255)

                                         tcGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTgg                                                                                                    >  1:963622/1‑141 (MQ=255)

                                           gAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGAt                                                                                                  >  1:494594/1‑141 (MQ=255)

                                            aGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATa                                                                                                 >  2:158862/1‑141 (MQ=255)

                                            aGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATa                                                                                                 <  2:1041529/141‑1 (MQ=255)

                                             ggACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAg                                                                                                >  2:416426/1‑141 (MQ=255)

                                               aCTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGaa                                                                                              >  1:236681/1‑141 (MQ=255)

                                                  tataTGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATa                                                                                           <  1:620810/141‑1 (MQ=255)

                                                   atatGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATac                                                                                          >  1:294706/1‑141 (MQ=255)

                                                   atatGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATac                                                                                          <  1:921901/141‑1 (MQ=255)

                                                      tgataaaGAAGGATATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACg                                                                                       <  2:278741/137‑1 (MQ=14)

                                                      tGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACg                                                                                       <  1:127228/141‑1 (MQ=255)

                                                       gataaaGAAGGATATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACga                                                                                      <  1:146621/138‑2 (MQ=14)

                                                        aCAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACGCGTa                                                                                     >  2:526887/1‑141 (MQ=255)

                                                            agaagGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAGTGATGGTATGATTTTAAATTTGGATAGAAATACACATACGGc                                                                                 >  2:1038806/1‑141 (MQ=255)

                                                             gaagGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAAGAAACCTGGTGGATGAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGcc                                                                                >  1:319186/1‑141 (MQ=255)

                                                                ggATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAg                                                                             >  1:577650/1‑141 (MQ=255)

                                                                 gATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAgg                                                                            >  2:212276/1‑141 (MQ=255)

                                                                  atatCGAGCGAGAGACATTATAAAGGGTGCAAAAGGGATGTGGATGATGTATTCAGATTTCGCCAAAAGTAATGAACCGGGTGTATTAAATACTGACGGTATGATGTGGAACTTGGATAGAAATACAAGTACGGCCACggg                                                                           >  2:226255/1‑141 (MQ=12)

                                                                   tatCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAgggc                                                                          >  1:546414/1‑140 (MQ=255)

                                                                   tatCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGa                                                                          <  2:230217/141‑1 (MQ=255)

                                                                   tatCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGa                                                                          >  2:943055/1‑141 (MQ=255)

                                                                    atCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGat                                                                         <  1:416426/141‑1 (MQ=255)

                                                                    atCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGat                                                                         >  1:460827/1‑141 (MQ=255)

                                                                     tCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGata                                                                        <  2:715156/141‑1 (MQ=255)

                                                                     tCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGata                                                                        >  2:599077/1‑141 (MQ=255)

                                                                      cGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGatat                                                                       <  2:96860/141‑1 (MQ=255)

                                                                         gaTAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGAtattat                                                                    >  1:976333/1‑141 (MQ=255)

                                                                           tAGCTAATATAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTATATAAGGAAGGTATTATTGGAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTAttt                                                                  <  2:914722/141‑1 (MQ=21)

                                                                            ggCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACGAACGGCCAAGGGATATTAtttt                                                                 <  2:239550/140‑1 (MQ=18)

                                                                            aGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTAtttt                                                                 >  1:768538/1‑141 (MQ=255)

                                                                              cGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGt                                                               <  2:560078/141‑1 (MQ=255)

                                                                                 aTTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGa                                                            >  2:1011315/1‑141 (MQ=255)

                                                                                  tttAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGAt                                                           >  2:896020/1‑141 (MQ=255)

                                                                                    tAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATAc                                                         <  2:570421/141‑1 (MQ=255)

                                                                                    tAAAAAAGGTGACAAAGAGATGTGGATGATATATACAGATTTAGACAAAAGAAATCATTCGGCTGTTTTGGAAAATGAAGGTATGCTTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATAc                                                         <  1:542214/141‑1 (MQ=255)

                                                                                     aaaaaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTAGTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGACATTATTTTGTAGATACt                                                        >  1:384309/1‑141 (MQ=255)

                                                                                      aaaaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACta                                                       >  1:726845/1‑141 (MQ=255)

                                                                                        aaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACtata                                                     >  1:597303/1‑141 (MQ=255)

                                                                                        aaaGGTGACAAAGGGAAGTGGATGATATATACAGATTTAGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTTGAGAAAAATAAACGAACGGCGAAGGGATATTATTTTGTAGATACtaaa                                                     >  2:84596/1‑139 (MQ=21)

                                                                                         aaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACtatat                                                    <  2:493842/141‑1 (MQ=255)

                                                                                           ggTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTatatat                                                  <  2:747606/141‑1 (MQ=255)

                                                                                             tGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGa                                                <  2:63758/141‑1 (MQ=255)

                                                                                              gACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGAc                                               >  1:727708/1‑141 (MQ=255)

                                                                                              gACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGAc                                               >  1:389279/1‑141 (MQ=255)

                                                                                              gACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGAc                                               >  2:506568/1‑141 (MQ=255)

                                                                                                cAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACaa                                             >  2:482676/1‑141 (MQ=255)

                                                                                                   aGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCa                                          <  2:995216/141‑1 (MQ=255)

                                                                                                     ggATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATg                                        <  1:726318/141‑1 (MQ=255)

                                                                                                      gATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGa                                       <  2:364276/141‑1 (MQ=255)

                                                                                                       aTGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGaa                                      >  1:662567/1‑141 (MQ=255)

                                                                                                       aTGTGGATGATATATACAGATTTCGACAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGaa                                      <  2:583078/141‑1 (MQ=255)

                                                                                                        tgtgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGaaa                                     >  1:535495/1‑141 (MQ=255)

                                                                                                          tgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAc                                   >  1:877558/1‑141 (MQ=255)

                                                                                                          tgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAc                                   >  1:197223/1‑141 (MQ=255)

                                                                                                          tgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAc                                   >  2:661428/1‑141 (MQ=255)

                                                                                                             atgatATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAActct                                <  1:198234/141‑1 (MQ=255)

                                                                                                             atgatATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTAATAGATAAAGAAGGTATGATCTAAAATTTGTGCAGCAATTCACGGACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAActct                                >  1:1028898/1‑141 (MQ=37)

                                                                                                                  atataCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAg                           <  1:271465/141‑1 (MQ=255)

                                                                                                                   tataCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGt                          <  2:195877/141‑1 (MQ=255)

                                                                                                                   tataCAGACTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGt                          <  1:171359/141‑1 (MQ=255)

                                                                                                                      aCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTaaa                       >  2:755061/1‑141 (MQ=255)

                                                                                                                           tttCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATta                  <  2:1001192/141‑1 (MQ=255)

                                                                                                                             tCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATtata                >  1:747997/1‑141 (MQ=255)

                                                                                                                             tCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATtata                >  1:856371/1‑141 (MQ=255)

                                                                                                                                 cAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACGAACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGt            >  1:173025/1‑141 (MQ=38)

                                                                                                                                 cAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGt            >  1:1000517/1‑141 (MQ=255)

                                                                                                                                        aataaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGAta     >  2:23093/1‑140 (MQ=255)

                                                                                                                                        aataaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATAATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATg     <  2:250849/141‑1 (MQ=255)

                                                                                                                                        aataaAACGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAAAAAAAAAAAAAAAATTATAGAGTTGAGATg     <  2:5017/141‑1 (MQ=255)

                                                                                                                                         ataaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGa    >  1:688957/1‑141 (MQ=255)

                                                                                                                                          taaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGaa   >  1:858620/1‑141 (MQ=255)

                                                                                                                                           aaaCCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGGTGAGATGaaa  >  2:941145/1‑141 (MQ=255)

GAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAA  >  CP000730/464455‑464734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`