BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	545,072	A→T	5.2%	S131S (TCA→TCT)	yabR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	545,072	0	A	T	5.2%	361.0 / 8.1	116	S131S (TCA→TCT)	yabR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (58/51); new base T (4/2); total (63/53)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 116 total aligned reads displayed.

AGTAAACCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTAATGA  >  CP000730/544935‑545209

aGTAAACCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGa                                                                                                                                        >  2:26961/1‑141 (MQ=255)

  tAAACCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACg                                                                                                                                      <  1:862377/141‑1 (MQ=255)

   aaaCCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGt                                                                                                                                     <  2:508579/141‑1 (MQ=255)

   aaaCCAAATCATCAAAAACCAGTGCAAAAGGCCGAAGATTTTGAAAAGAAATTAAGCAAATTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGt                                                                                                                                     <  2:933138/141‑1 (MQ=255)

    aaCCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGtt                                                                                                                                    >  1:385780/1‑141 (MQ=255)

    aaCCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGtt                                                                                                                                    >  2:1004257/1‑141 (MQ=255)

     aCCAAGTCCTCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAAACAAACGTCAAACAGAATCTAGACGCGGTGGCAACGGTTAAAGACGtta                                                                                                                                   >  1:351377/1‑141 (MQ=255)

      ccAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaa                                                                                                                                  >  1:14124/1‑141 (MQ=255)

      ccAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaa                                                                                                                                  <  1:815817/141‑1 (MQ=255)

      ccAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaa                                                                                                                                  <  1:400583/141‑1 (MQ=255)

      ccAAGTCATCAAAAACAAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaa                                                                                                                                  <  2:25698/141‑1 (MQ=255)

        aaGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaatt                                                                                                                                >  1:681909/1‑141 (MQ=255)

         aGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaatta                                                                                                                               >  2:314743/1‑141 (MQ=255)

         aGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGttaatta                                                                                                                               <  2:513865/141‑1 (MQ=255)

             atcaAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCTAGACGTTAATTAaaat                                                                                                                           <  2:197737/141‑1 (MQ=255)

             atcaAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaat                                                                                                                           >  1:365717/1‑141 (MQ=255)

             atcaAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaat                                                                                                                           >  2:182983/1‑141 (MQ=255)

             atcaAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaat                                                                                                                           <  2:930907/141‑1 (MQ=255)

             atcaAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaat                                                                                                                           >  1:807170/1‑141 (MQ=255)

              tcaAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaata                                                                                                                          >  2:348518/1‑141 (MQ=255)

               caAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaataa                                                                                                                         <  2:552833/141‑1 (MQ=255)

                 aaaaCCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAaaataaat                                                                                                                       >  1:1025340/1‑141 (MQ=255)

                    aCCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAAtaaataaa                                                                                                                    >  1:484755/1‑141 (MQ=255)

                      cAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAAAAAAGa                                                                                                                  >  1:304/1‑141 (MQ=255)

                       aGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGAc                                                                                                                 >  1:820716/1‑141 (MQ=255)

                         tGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTg                                                                                                               <  1:1034530/141‑1 (MQ=255)

                         tGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTg                                                                                                               <  2:477228/141‑1 (MQ=255)

                          gCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGc                                                                                                              >  2:1031670/1‑141 (MQ=255)

                           cAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCt                                                                                                             <  1:897536/141‑1 (MQ=255)

                                aGCAGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCTAGACGTTAATTAAAATAAATAAAGACTGTTTCGAt                                                                                                        <  2:1018465/141‑1 (MQ=255)

                                    gAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAgg                                                                                                    >  1:1013469/1‑141 (MQ=255)

                                     aaGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGa                                                                                                   >  1:789920/1‑141 (MQ=255)

                                        aTTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAata                                                                                                <  1:668193/141‑1 (MQ=255)

                                        aTTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAata                                                                                                >  1:895644/1‑141 (MQ=255)

                                        aTTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAata                                                                                                <  2:555960/141‑1 (MQ=255)

                                         ttttGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAatat                                                                                               <  2:363673/141‑1 (MQ=255)

                                           ttGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCTAGACGTTAATTAAAATAAATAAAGACTGTTTCGATAAGGAatatat                                                                                             >  2:397448/1‑141 (MQ=255)

                                           ttGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAatatat                                                                                             <  2:784048/141‑1 (MQ=255)

                                            tGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATAtt                                                                                            <  1:21720/141‑1 (MQ=255)

                                             gAAAAGAAAGTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATAttt                                                                                           >  1:952495/1‑141 (MQ=255)

                                              aaaaGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATGTATTTa                                                                                          >  1:511486/1‑141 (MQ=255)

                                              aaaaGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTa                                                                                          <  2:614105/141‑1 (MQ=255)

                                                aaGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCGAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGa                                                                                        >  1:226691/1‑141 (MQ=255)

                                                   aaaTTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAatg                                                                                     <  2:663244/141‑1 (MQ=255)

                                                        aaGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCg                                                                                >  1:729232/1‑141 (MQ=255)

                                                         aGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGt                                                                               >  1:815797/1‑141 (MQ=255)

                                                           cAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTAt                                                                             <  2:518363/141‑1 (MQ=255)

                                                            aaTTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATc                                                                            >  2:966003/1‑141 (MQ=255)

                                                               ttCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGaa                                                                         <  2:649740/141‑1 (MQ=255)

                                                                  ttAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGaataa                                                                      >  1:340201/1‑141 (MQ=255)

                                                                  ttAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGaataa                                                                      >  2:393669/1‑141 (MQ=255)

                                                                    aaaaTATAGTGAAGATAATTTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATATAGACTGCTTCGATAAGTTGTTTATTTAGAATGATTCGTATCGaataagc                                                                    <  2:373182/141‑3 (MQ=255)

                                                                    aaaaGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATc                                                                    <  2:423781/141‑1 (MQ=255)

                                                                      aaGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGa                                                                  >  1:458281/1‑141 (MQ=255)

                                                                         tgattGAAGTTAATTTATCTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTg                                                               >  2:719669/5‑141 (MQ=255)

                                                                          tAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGc                                                              >  2:251389/1‑141 (MQ=255)

                                                                              gAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCg                                                          >  1:27059/1‑141 (MQ=255)

                                                                               aaGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGt                                                         <  1:1001240/141‑1 (MQ=255)

                                                                                  aTAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAg                                                      <  1:894353/141‑1 (MQ=255)

                                                                                    aaaTTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTTCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGAc                                                    <  2:1085036/141‑1 (MQ=255)

                                                                                    aaaTTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGAc                                                    <  2:1069230/141‑1 (MQ=255)

                                                                                        tAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATc                                                >  1:641376/1‑141 (MQ=255)

                                                                                        tAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGAGTTAGACAATc                                                >  2:807974/1‑141 (MQ=255)

                                                                                         aaCTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCt                                               >  1:743792/1‑141 (MQ=255)

                                                                                          aCTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTa                                              >  1:72991/1‑141 (MQ=255)

                                                                                          aCTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTa                                              <  1:386384/141‑1 (MQ=255)

                                                                                           cTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTATAATGATGCGTATCGAAGAATCGATTGCAGCGTTAGACAATATaa                                             >  2:434519/1‑141 (MQ=255)

                                                                                           cTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTaa                                             <  2:895644/141‑1 (MQ=255)

                                                                                            ttCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAg                                            <  2:325409/141‑1 (MQ=255)

                                                                                             tcaatcaaACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTc                                                                                                           >  2:398382/1‑77 (MQ=255)

                                                                                              caatcaaACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGAc                                          <  1:79984/141‑1 (MQ=255)

                                                                                               aatcaaACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACt                                         <  1:6681/141‑1 (MQ=255)

                                                                                                atcaaACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTg                                        <  2:359323/141‑1 (MQ=255)

                                                                                                 tcaaACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGt                                       >  2:903895/1‑141 (MQ=255)

                                                                                                    aaCGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTc                                    <  2:1052080/141‑1 (MQ=255)

                                                                                                     aCGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCTAGACGTTAATTAAAATAAATAAAGACTGTTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCt                                   >  1:994165/1‑141 (MQ=255)

                                                                                                     aCGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCt                                   >  1:1030418/1‑141 (MQ=255)

                                                                                                     aCGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCt                                   <  1:819894/141‑1 (MQ=255)

                                                                                                      cGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCtt                                  >  1:22097/1‑141 (MQ=255)

                                                                                                      cGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCtt                                  >  2:828524/1‑141 (MQ=255)

                                                                                                       gTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTa                                 <  1:614389/141‑1 (MQ=255)

                                                                                                        tCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTaa                                >  2:729384/1‑141 (MQ=255)

                                                                                                        tCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTaa                                >  1:271929/1‑141 (MQ=255)

                                                                                                        tCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTaa                                <  2:886548/141‑1 (MQ=255)

                                                                                                         cAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTaaa                               <  1:514857/141‑1 (MQ=255)

                                                                                                          aaaCAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAAt                              >  1:703698/1‑141 (MQ=255)

                                                                                                          aaaCAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGGTTCTTAAAt                              >  1:979700/1‑141 (MQ=255)

                                                                                                           aaCAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATa                             >  2:472279/1‑141 (MQ=255)

                                                                                                             cAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAg                           >  1:192796/1‑141 (MQ=255)

                                                                                                                  tCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCa                      >  1:790928/1‑141 (MQ=255)

                                                                                                                   cTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAg                     <  2:825178/141‑1 (MQ=255)

                                                                                                                      gACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGtct                  <  2:407165/141‑1 (MQ=255)

                                                                                                                       aagcgGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGtctc                 <  2:766659/139‑1 (MQ=255)

                                                                                                                        cgcgGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTctct                >  2:176416/1‑141 (MQ=255)

                                                                                                                          cgGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCttt              <  1:461007/141‑1 (MQ=255)

                                                                                                                                cAAAGGTTCTAGACGTTAATTAAAATAAATAAAGACTGTTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTg        >  1:908648/1‑141 (MQ=255)

                                                                                                                                cAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACTATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTg        >  1:852293/1‑141 (MQ=255)

                                                                                                                                  aaGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTa      <  1:251220/141‑1 (MQ=255)

                                                                                                                                     gTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATGTATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTAATg   <  2:511486/141‑1 (MQ=255)

                                                                                                                                      ttCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTAATGa  <  2:498472/141‑1 (MQ=255)

AGTAAACCAAGTCATCAAAAACCAGTGCAAAAAGCCGAAGATTTTGAAAAGAAATTAAGCAATTTCTTAAAAGATAGTGAAGATAAATTAACTTCAATCAAACGTCAAACAGAATCTAGACGCGGTGGCAAAGGTTCAAGACGTTAATTAAAATAAATAAAGACTGCTTCGATAAGGAATATATTTAGAATGATGCGTATCGAATAATCGATTGCAGCGTTAGACAATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTAATGA  >  CP000730/544935‑545209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`