Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 604,295 T→C 6.7% intergenic (+88/‑126) araB → / → USA300HOU_0546 ribulokinase/possible UDP‑glucose 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730604,2950TC 6.7% 254.1 / 7.2 90intergenic (+88/‑126)araB/USA300HOU_0546ribulokinase/possible UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (41/43);  new base C (3/3);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

CTAATATAATGAAAGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGAAAAAAATTAT  >  CP000730/604164‑604434
                                                                                                                                   |                                                                                                                                           
cTAATATAATGAAAGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAac                                                                                                                                    >  1:1002919/1‑141 (MQ=255)
    tataATGAAAGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAacatgt                                                                                                                                <  2:1031377/141‑1 (MQ=255)
    tataATGAAAGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAacatgt                                                                                                                                <  2:210335/141‑1 (MQ=255)
      taATGAAAGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATGTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAacatgtac                                                                                                                              <  1:853509/141‑1 (MQ=255)
          gAAAGATATCCAGAGTTAAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAacatgtacatgt                                                                                                                          >  2:136274/1‑141 (MQ=255)
          aaaaGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAacatgtacatgt                                                                                                                          <  1:814822/140‑1 (MQ=255)
            aaGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCa                                                                                                                        <  1:607402/141‑1 (MQ=255)
            aaGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCa                                                                                                                        >  1:634085/1‑141 (MQ=255)
            aaGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCa                                                                                                                        <  1:2035/141‑1 (MQ=255)
            aaGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCa                                                                                                                        <  2:831063/141‑1 (MQ=255)
             aGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCat                                                                                                                       >  1:383185/1‑141 (MQ=255)
             aGATATCCAGAGTTTAAGAGCTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATATACGCTATGAATTACTAAAATTAATGAATAGAACTAGAAATTCCATTTAAAAAAATATCAGAAACATGTACATGTCat                                                                                                                       >  2:136588/1‑141 (MQ=255)
                  tCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAACATCAGCAACATGTACATGTCAtatata                                                                                                                  >  1:49801/1‑141 (MQ=255)
                    cAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACa                                                                                                                >  2:178592/1‑141 (MQ=255)
                          ttAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAACATCAGCAACATGTACATGTCATATATACAAGAAAg                                                                                                          >  2:736308/1‑141 (MQ=255)
                           tAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAACATCAGCAACATGTACATGTCATATATACAAGAAAgc                                                                                                         <  2:14674/141‑1 (MQ=255)
                            aaGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGTATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAgcg                                                                                                        >  2:898599/1‑141 (MQ=255)
                              gagTTAAGGGATAAGAAATTTAATTTGACAGTAAGTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGCATTTCCAATTACAGATATATCAGCAACATGTACGTGTCATATATACAAGAAAGCGCt                                                                                                      <  2:279363/141‑1 (MQ=255)
                               agTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCtt                                                                                                     <  2:806249/141‑1 (MQ=255)
                                gTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCttt                                                                                                    <  2:1026400/141‑1 (MQ=255)
                                   gAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTttat                                                                                                 >  2:68895/1‑141 (MQ=255)
                                     gggATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTttatat                                                                                               >  2:574097/1‑141 (MQ=255)
                                       gATAAATAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATTAATTAATACAATTAGAAGTTCCATTTACAGAAATATCAGCAACATTTACATGTCATATCTACAAGAAAGCGCTTttatatta                                                                                             <  2:557526/141‑1 (MQ=255)
                                          aaaaaaTTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATaa                                                                                          <  1:922304/141‑1 (MQ=255)
                                          aaaaaaTTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATaa                                                                                          >  2:196329/1‑141 (MQ=255)
                                             aaaTTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAatta                                                                                       >  1:574455/1‑141 (MQ=255)
                                             aaaTTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAatta                                                                                       >  1:134000/1‑141 (MQ=255)
                                               atttaatttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAattatt                                                                                     >  1:63021/1‑141 (MQ=255)
                                                tttaatttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAAttatta                                                                                    <  2:537417/141‑1 (MQ=255)
                                                 ttaatttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATtattat                                                                                   >  1:830610/1‑141 (MQ=255)
                                                 ttaatttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATtattat                                                                                   <  1:943306/141‑1 (MQ=255)
                                                 ttaatttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAACATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATtattat                                                                                   <  1:421391/141‑1 (MQ=255)
                                                    atttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGaa                                                                                >  2:547693/1‑141 (MQ=255)
                                                    atttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGaa                                                                                >  2:930953/1‑141 (MQ=255)
                                                     tttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATATTTATTATGaaa                                                                               >  2:309643/1‑141 (MQ=255)
                                                     tttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGaaa                                                                               <  2:161610/141‑1 (MQ=255)
                                                     tttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGaaa                                                                               >  2:287874/1‑141 (MQ=255)
                                                     tttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGaaa                                                                               >  1:862319/1‑141 (MQ=255)
                                                      ttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATTTATACAAGAAAGCGCTTTTATATTATAATTATTATGaaaa                                                                              <  2:934962/141‑1 (MQ=255)
                                                      ttGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGaaaa                                                                              <  1:899099/141‑1 (MQ=255)
                                                        gACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATg                                                                            >  1:101099/1‑141 (MQ=255)
                                                             tAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAatat                                                                       <  1:551953/141‑1 (MQ=255)
                                                              aaTTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAAtatt                                                                      <  1:896757/141‑1 (MQ=255)
                                                              aaTTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAAtatt                                                                      >  1:165652/1‑141 (MQ=255)
                                                              aaTTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAAtatt                                                                      >  1:761205/1‑141 (MQ=255)
                                                                 tAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATg                                                                   >  1:116127/1‑141 (MQ=255)
                                                                  aGCAATAATAAACGCTATAAATTACTAAAATCAGTTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTATATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATgg                                                                  <  2:861027/141‑2 (MQ=255)
                                                                  aGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGt                                                                  >  1:310456/1‑141 (MQ=255)
                                                                    cAATATTAAACGCTATAAATTACTAAAATCAATTAATACATCTCGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGGAAGCGCATGTATATTATAACTATTATGATCATGAATATTATGTcc                                                                <  2:165986/141‑1 (MQ=255)
                                                                    cAATAATAAACGATATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTcc                                                                <  2:934179/141‑1 (MQ=255)
                                                                     aataatAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCg                                                               <  1:693366/141‑1 (MQ=255)
                                                                      ataataAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGc                                                              >  2:645453/1‑141 (MQ=255)
                                                                      ataataAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGc                                                              >  2:274549/1‑141 (MQ=255)
                                                                      ataataAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGc                                                              >  2:274544/1‑141 (MQ=255)
                                                                       taataaACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCt                                                             >  1:57087/1‑141 (MQ=255)
                                                                       taataaACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCt                                                             <  2:913340/141‑1 (MQ=255)
                                                                       taataaACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCt                                                             <  2:199979/141‑1 (MQ=255)
                                                                       aaataaACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCt                                                             <  2:782662/140‑1 (MQ=255)
                                                                           aaaCGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCAGCTtgtg                                                         >  1:446170/1‑141 (MQ=255)
                                                                             aCGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCATCATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGAt                                                       <  2:574455/141‑1 (MQ=255)
                                                                              cGCTATAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATgg                                                     >  1:74471/1‑141 (MQ=255)
                                                                               gCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAACATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGACgg                                                     >  1:895829/1‑141 (MQ=255)
                                                                                 tataAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGAc                                                   <  2:1002919/141‑1 (MQ=255)
                                                                                  ataAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTccatcacaagcggaca                                                  <  2:334490/141‑15 (MQ=255)
                                                                                     aaTTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACtata                                               <  2:266647/141‑1 (MQ=255)
                                                                                       ttACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGc                                             <  2:1006574/141‑1 (MQ=255)
                                                                                           tAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCtata                                         <  1:1000370/141‑1 (MQ=255)
                                                                                                 cAATTAATACAACTATAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATagaag                                   <  2:888601/141‑1 (MQ=255)
                                                                                                 cAATTAATACAACTAGAATTTCCATTTACAGAAACATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGACGGACTATAGCTATATagaag                                   <  1:736308/141‑1 (MQ=255)
                                                                                                    ttAATACGACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAgaga                                >  1:860197/1‑141 (MQ=255)
                                                                                                    ttAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAgaga                                >  2:485833/1‑141 (MQ=255)
                                                                                                    ttAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAgaga                                >  1:888949/1‑141 (MQ=255)
                                                                                                      aaTACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACa                              >  1:333846/1‑141 (MQ=255)
                                                                                                           aaCTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAATGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGTCAAAGga                         <  2:310374/141‑1 (MQ=255)
                                                                                                           aaCTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGga                         <  1:168964/141‑1 (MQ=255)
                                                                                                           aaCTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGga                         >  2:833550/1‑141 (MQ=255)
                                                                                                           aaCTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGga                         <  1:447161/141‑1 (MQ=255)
                                                                                                             cTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGgaga                       >  1:599455/1‑141 (MQ=255)
                                                                                                               aGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGata                     >  2:513520/1‑141 (MQ=255)
                                                                                                                    ttCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCt                >  1:328564/1‑141 (MQ=255)
                                                                                                                       cATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATg             <  2:414220/141‑1 (MQ=255)
                                                                                                                       cATTTAAAGAAATATCAGCAACATGTACATGTCATATAAACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATg             <  1:67323/141‑1 (MQ=255)
                                                                                                                        aTTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGa            >  1:211987/1‑141 (MQ=255)
                                                                                                                        aTTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGa            <  2:399584/141‑1 (MQ=255)
                                                                                                                        aTTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGa            <  1:79873/141‑1 (MQ=255)
                                                                                                                         tttACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGaa           >  2:406810/1‑141 (MQ=255)
                                                                                                                             cAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGaaaaaa       <  1:404646/141‑1 (MQ=255)
                                                                                                                             cAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGaaaaaa       >  1:872365/1‑141 (MQ=255)
                                                                                                                                aaaTATCAGCATCATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGTTATTTCTATGAAAAAAAtt    <  2:754059/141‑1 (MQ=255)
                                                                                                                                aaaTATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGAAAAAAAtt    <  1:639266/141‑1 (MQ=255)
                                                                                                                                  atatCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGAAAAAAATTAt  >  1:154164/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                           
CTAATATAATGAAAGATATCCAGAGTTTAAGAGTTGAGGGATAAAAAATTTAATTTGACAGTAATTAGCAATAATAAACGCTATAAATTACTAAAATCAATTAATACAACTAGAATTTCCATTTACAGAAATATCAGCAACATGTACATGTCATATATACAAGAAAGCGCTTTTATATTATAATTATTATGAAAATGAATATTATGTCCGCTTGTGATGGACTATAGCTATATAGAAGAGACAAAGGAGATATTTCTATGAAAAAAATTAT  >  CP000730/604164‑604434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: