Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 696,941 G→A 6.1% A303A (GCG→GCA mnhA1 → CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730696,9410GA 6.1% 248.9 / 3.7 83A303A (GCG→GCAmnhA1CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit
Reads supporting (aligned to +/- strand):  ref base G (38/39);  new base A (3/2);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAAT  >  CP000730/696802‑697076
                                                                                                                                           |                                                                                                                                       
tCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGt                                                                                                                                        <  2:961719/141‑1 (MQ=255)
   ttttATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAct                                                                                                                                     <  1:52670/141‑1 (MQ=255)
    tttATTACTTCTATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTActc                                                                                                                                    >  2:596732/1‑141 (MQ=255)
       attaCTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATTACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTAc                                                                                                                                 >  1:1029732/1‑141 (MQ=255)
             tCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAg                                                                                                                           <  1:841944/141‑1 (MQ=255)
              cGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGt                                                                                                                          <  1:413776/141‑1 (MQ=255)
               gATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTc                                                                                                                         <  1:339341/141‑1 (MQ=255)
                   tACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACt                                                                                                                     >  2:483351/1‑141 (MQ=255)
                     cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                   >  2:763660/1‑141 (MQ=255)
                     cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                   >  1:850539/1‑141 (MQ=255)
                     cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                   >  2:718277/1‑141 (MQ=255)
                     cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                   >  2:499583/1‑141 (MQ=255)
                        cATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGga                                                                                                                <  1:166746/141‑1 (MQ=255)
                         attattAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGgat                                                                                                               >  2:477830/1‑141 (MQ=255)
                           tattaGGTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGaatga                                                                                                             <  2:50171/141‑1 (MQ=255)
                               aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                         >  1:1046528/1‑141 (MQ=255)
                               aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                         >  1:1046372/1‑141 (MQ=255)
                               aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                         <  1:87939/141‑1 (MQ=255)
                                 gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                       >  2:406734/1‑141 (MQ=255)
                                 gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                       >  1:212725/1‑141 (MQ=255)
                                 gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                       >  1:526260/1‑141 (MQ=255)
                                 gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                                                                                                       <  1:472694/141‑1 (MQ=255)
                                 gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAAAGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                                                                                                       >  2:409041/1‑141 (MQ=255)
                                        cAATATGTACGTATATATCGTTACGCTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGCTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggt                                                                                                >  2:949593/1‑141 (MQ=255)
                                         aaTATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATAACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggta                                                                                               <  1:1028510/141‑2 (MQ=255)
                                             tGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATTAGTCAACTTGGGATGATTATGGCTATGGTGGGta                                                                                           >  1:534463/1‑141 (MQ=255)
                                             tGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATGGCAATGGTCGGta                                                                                           >  2:790719/1‑141 (MQ=255)
                                               tACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGtata                                                                                         >  1:366526/1‑141 (MQ=255)
                                                aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg                                                                                        <  2:792135/141‑1 (MQ=255)
                                                aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg                                                                                        <  2:688604/141‑1 (MQ=255)
                                                  gTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGt                                                                                      >  2:66758/1‑141 (MQ=255)
                                                   tatataTCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTg                                                                                     <  2:269464/141‑1 (MQ=255)
                                                    atatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTgg                                                                                    <  2:850633/141‑1 (MQ=255)
                                                     tatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAAGAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGc                                                                                   <  1:661922/141‑1 (MQ=255)
                                                        ttCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                                <  1:891516/140‑1 (MQ=255)
                                                        atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                                <  1:183151/141‑1 (MQ=255)
                                                        atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                                <  1:943320/141‑1 (MQ=255)
                                                         tCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGat                                                                               <  2:240271/141‑1 (MQ=255)
                                                         tCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTAAACTAGGAATGATTATGGCAATGGTCGGTATCGGTGGTGGat                                                                               >  1:204624/1‑141 (MQ=255)
                                                          cGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGata                                                                              <  2:1040827/141‑1 (MQ=255)
                                                           gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat                                                                             <  2:453683/141‑1 (MQ=255)
                                                           gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat                                                                             >  1:406326/1‑141 (MQ=255)
                                                            ttACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATg                                                                            <  2:302176/141‑1 (MQ=255)
                                                             tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                           >  2:400823/1‑141 (MQ=255)
                                                             tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                           >  1:97988/1‑141 (MQ=255)
                                                             tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                           >  1:661539/1‑141 (MQ=255)
                                                                gTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCa                                                                        >  1:993416/1‑141 (MQ=255)
                                                                 tttGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGATTTATAAAATTGGGATTTAATTGATGACTTAGTCTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCaa                                                                       <  1:647594/141‑1 (MQ=255)
                                                                 taattgaaccaaataaCATTGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCaa                                                                       >  2:378934/12‑141 (MQ=255)
                                                                  ttgttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAac                                                                      >  1:404146/1‑141 (MQ=255)
                                                                   tgttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAaca                                                                     <  1:234458/141‑1 (MQ=255)
                                                                     ttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACAcc                                                                   <  1:596732/141‑1 (MQ=255)
                                                                          ttAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACcaacaa                                                              <  1:972508/141‑1 (MQ=255)
                                                                           taataaCAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAg                                                             >  1:688480/1‑141 (MQ=255)
                                                                               aaCAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGc                                                         >  1:537701/1‑141 (MQ=255)
                                                                                  aaTGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAAt                                                      <  2:290607/141‑1 (MQ=255)
                                                                                     gTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGc                                                   <  2:163246/141‑1 (MQ=255)
                                                                                        aTTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATc                                                >  1:46561/1‑141 (MQ=255)
                                                                                            ggTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTAtt                                            <  1:842925/141‑1 (MQ=255)
                                                                                             gTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTAttt                                           >  2:880435/1‑141 (MQ=255)
                                                                                               tCAATTCCAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAATCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAt                                         <  1:867402/141‑1 (MQ=255)
                                                                                               tCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAt                                         <  1:1084320/141‑1 (MQ=255)
                                                                                                caaatACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtg                                        <  2:285899/137‑1 (MQ=255)
                                                                                                cAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtg                                        >  2:804408/1‑141 (MQ=255)
                                                                                                  aTTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtgta                                      >  2:245824/1‑141 (MQ=255)
                                                                                                        gCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtgtatttgta                                >  1:705877/1‑141 (MQ=255)
                                                                                                            tAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGtattat                            >  1:406223/1‑141 (MQ=255)
                                                                                                                    tGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcg                    <  1:1075130/141‑1 (MQ=255)
                                                                                                                    tGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcg                    <  2:86087/141‑1 (MQ=255)
                                                                                                                     gggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc                   <  2:177707/141‑1 (MQ=255)
                                                                                                                     gggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc                   <  1:628697/141‑1 (MQ=255)
                                                                                                                     gggATTTAAAAGGTAGCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACGCCAACATGGCGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc                   <  2:209791/141‑1 (MQ=255)
                                                                                                                      ggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCt                  <  2:32194/141‑1 (MQ=255)
                                                                                                                       gATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTa                 >  1:269434/1‑141 (MQ=255)
                                                                                                                        aTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTAt                >  2:234547/1‑141 (MQ=255)
                                                                                                                        aTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTAt                >  1:347279/1‑141 (MQ=255)
                                                                                                                           tAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTc             <  1:378934/141‑1 (MQ=255)
                                                                                                                             aaaGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCAt           <  2:178637/141‑1 (MQ=255)
                                                                                                                                ggTATCCTAGCGTACTCTACAATCAGTCAACTTGGCATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTa        >  1:948642/1‑141 (MQ=255)
                                                                                                                                 gTATCCTAGCGTACTCTACAATCAGTCAACTTTGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTaa       <  1:60361/141‑1 (MQ=255)
                                                                                                                                 gTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTaa       >  1:626406/1‑141 (MQ=255)
                                                                                                                                  tATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAAt      <  2:491616/141‑1 (MQ=255)
                                                                                                                                      cTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAAt  >  1:281671/1‑141 (MQ=255)
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TCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAAT  >  CP000730/696802‑697076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: