Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 888,334 T→A 5.5% V219E (GTA→GAA)  aroD → 3‑dehydroquinate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730888,3340TA 5.5% 285.1 / 4.4 96V219E (GTA→GAA) aroD3‑dehydroquinate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (48/41);  new base A (4/2);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATTGCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCGATTT  >  CP000730/888198‑888474
                                                                                                                                        |                                                                                                                                             
ttATTGCAGGCAATGTCTACATTTTCAGATACTATGGATTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGACCGGCTCAAGGCGTTTTTGGTCGCGCGGTTACTTGAGGCTGTTTCGGAGGACca                                                                                                                                           <  2:273643/141‑1 (MQ=255)
  aTTGCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACcaca                                                                                                                                         <  1:166187/141‑1 (MQ=255)
   ttGCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACaa                                                                                                                                        >  1:684193/1‑141 (MQ=255)
   ttGCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACaa                                                                                                                                        >  2:616431/1‑141 (MQ=255)
    tGCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGTTTGTGTGTTTACTTATGGTTGTATCGGAGTACCACAAg                                                                                                                                       >  2:566261/1‑141 (MQ=255)
     gCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGc                                                                                                                                      <  1:190012/141‑1 (MQ=255)
       aGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTc                                                                                                                                    >  1:954815/1‑141 (MQ=255)
        ggCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTcc                                                                                                                                   >  1:915869/1‑141 (MQ=255)
        ggCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTCCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTcc                                                                                                                                   <  1:784089/141‑1 (MQ=255)
          cAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAg                                                                                                                                 <  1:388774/141‑1 (MQ=255)
           aaTGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAgg                                                                                                                                <  1:619106/141‑1 (MQ=255)
            aTGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGa                                                                                                                               <  2:1068681/141‑1 (MQ=255)
                 tACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGAt                                                                                                                          <  1:848810/141‑1 (MQ=255)
                  aCATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGAtt                                                                                                                         <  2:917748/141‑1 (MQ=255)
                  aCATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGAtt                                                                                                                         <  1:500076/141‑1 (MQ=255)
                   cATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTg                                                                                                                        <  2:951582/141‑1 (MQ=255)
                     ttttCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGAt                                                                                                                      <  2:135195/141‑1 (MQ=255)
                     ttttCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGAt                                                                                                                      <  2:135210/141‑1 (MQ=255)
                     ttttCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGAt                                                                                                                      <  1:480191/141‑1 (MQ=255)
                     ttttCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGAt                                                                                                                      <  2:19093/141‑1 (MQ=255)
                         cAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTa                                                                                                                  <  2:587406/141‑1 (MQ=255)
                         cAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTa                                                                                                                  <  1:609800/141‑1 (MQ=255)
                          aGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTAc                                                                                                                 <  1:731261/141‑1 (MQ=255)
                          aGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTAc                                                                                                                 <  1:1041660/141‑1 (MQ=255)
                           gATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGAc                                                                                                                              <  1:108700/127‑1 (MQ=255)
                           gATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGAc                                                                                                                              >  2:108700/1‑127 (MQ=255)
                           gATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACt                                                                                                                <  1:763890/141‑1 (MQ=255)
                            aTACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTg                                                                                                               <  1:784349/141‑1 (MQ=255)
                                tATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGAttt                                                                                                           >  1:307441/1‑141 (MQ=255)
                                   ggACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTaaa                                                                                                        <  2:924653/141‑1 (MQ=255)
                                     aCTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAg                                                                                                      >  1:641943/1‑141 (MQ=255)
                                     aCTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAg                                                                                                      <  1:459188/141‑1 (MQ=255)
                                       tGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGca                                                                                                    >  2:618898/1‑141 (MQ=255)
                                         cAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTTTTTATTTGTTAACATAAAGTGTTAACGGTGAATCACAAACACCAGGACAGATTGAAGCTACTGTTTTAAAAGcaca                                                                                                  >  2:476683/1‑141 (MQ=255)
                                         cAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGcaca                                                                                                  >  1:349609/1‑141 (MQ=255)
                                            aGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGATCGGCTCACGGCGTTTTTGGTGGTGTGTTGGCATATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGt                                                                                               <  1:487786/141‑1 (MQ=255)
                                            aGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGt                                                                                               <  2:350901/141‑1 (MQ=255)
                                            aGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGt                                                                                               <  2:768757/141‑1 (MQ=255)
                                              ttgttgGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGa                                                                                             <  1:130776/141‑1 (MQ=255)
                                                 ttgGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACtt                                                                                          >  1:279328/1‑141 (MQ=255)
                                                  tgGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACttt                                                                                         >  1:608063/1‑141 (MQ=255)
                                                  tgGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACttt                                                                                         >  1:607993/1‑141 (MQ=255)
                                                   ggTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTta                                                                                        >  1:1038855/1‑141 (MQ=255)
                                                     tATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTtata                                                                                      <  1:106661/141‑1 (MQ=255)
                                                      aTTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCATTGACTTATGGTTGTATCGGAGAACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATAc                                                                                     <  2:714421/141‑1 (MQ=255)
                                                           aaTGTCTAAACTTGGACTAATAAGTAGAGCGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACtaata                                                                                >  2:324181/1‑141 (MQ=255)
                                                           aaTGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACtaata                                                                                <  2:681531/141‑1 (MQ=255)
                                                           aaTGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACtaata                                                                                >  1:49015/1‑141 (MQ=255)
                                                            aTGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACtaataa                                                                               >  1:611831/1‑141 (MQ=255)
                                                               tCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAAt                                                                            <  2:337388/141‑1 (MQ=255)
                                                                cTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATg                                                                           >  2:66898/1‑141 (MQ=255)
                                                                 tAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGa                                                                          >  1:1034298/1‑141 (MQ=255)
                                                                   aaCTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAAt                                                                        >  2:512168/1‑141 (MQ=255)
                                                                   aaCTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCATTGACTTATGGTTGTATCGGAGAACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAAt                                                                        >  1:765832/1‑141 (MQ=255)
                                                                      ttGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTGGGGCAAATAGTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAGTTAg                                                                     >  1:293684/1‑141 (MQ=255)
                                                                      ttGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAg                                                                     <  1:1027212/141‑1 (MQ=255)
                                                                          aCTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTg                                                                >  2:1044674/1‑141 (MQ=255)
                                                                            taataaGTAGAACGGCTCAAGGCGTTTTTGGTGGTTCGTTGACTAATGGTTGTATCGGAGAACAACAAGCTCCAGGAATGATTGGTGTTACTGGTTTAATAGCACTAGTGACTCGAGACTAATAAAATTAATTAGTTTGcg                                                              >  1:1024829/1‑140 (MQ=255)
                                                                            taataaGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCATTGACTTATGGTTGTATCGGAGAACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCt                                                              >  2:902949/1‑141 (MQ=255)
                                                                             aataaGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCtt                                                             >  2:25551/1‑141 (MQ=255)
                                                                                    aGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTa                                                      >  2:343939/1‑141 (MQ=255)
                                                                                     gAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGCTTGATGTTGCTAACTCCTACGCACCGGTGACTCCAAACTAATAAACTTCATTAGGTTGCTTTTGTGTaa                                                     >  1:643934/1‑141 (MQ=255)
                                                                                     gAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTaa                                                     >  1:1015349/1‑141 (MQ=255)
                                                                                        cGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTg                                                  <  2:16381/141‑1 (MQ=255)
                                                                                           cTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTg                                               >  1:481540/1‑141 (MQ=255)
                                                                                           cTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTg                                               >  1:161425/1‑141 (MQ=255)
                                                                                            tCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGt                                              >  1:430646/1‑141 (MQ=255)
                                                                                              aaGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCt                                            >  2:842027/1‑141 (MQ=255)
                                                                                              aaGGCGTTTTAGGAGGTGTGTTGACTTATGGTGGTATCGGAGTACCACACGCTCCAGGACAGATTGGCGTTACTGATTTAAAAGCACAAGGGACTTTAGAAGAATAAATTGAATAGGTGGGTTTTTGGGTAAGTGCTGGCt                                            >  2:861087/1‑141 (MQ=255)
                                                                                               aGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCtt                                           >  1:554366/1‑141 (MQ=255)
                                                                                                    tttttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAAt                                      >  2:1082047/1‑141 (MQ=255)
                                                                                                    tttttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAAt                                      >  1:278139/1‑141 (MQ=255)
                                                                                                    tttttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAAt                                      >  2:534585/1‑141 (MQ=255)
                                                                                                     ttttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAAtt                                     >  1:600976/1‑141 (MQ=255)
                                                                                                      tttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTg                                    >  1:450003/1‑141 (MQ=255)
                                                                                                      tttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTg                                    <  1:905194/141‑1 (MQ=255)
                                                                                                      tttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTg                                    <  1:429908/141‑1 (MQ=255)
                                                                                                      tttGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTg                                    >  2:176718/1‑141 (MQ=255)
                                                                                                              tgCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGtat                            >  2:982093/1‑141 (MQ=255)
                                                                                                              tgCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGtat                            <  2:443875/141‑1 (MQ=255)
                                                                                                               gCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGtatt                           >  2:1074889/1‑141 (MQ=255)
                                                                                                               gCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGtatt                           <  1:952390/141‑1 (MQ=255)
                                                                                                                cGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGtatta                          >  1:360136/1‑141 (MQ=255)
                                                                                                                cGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGtatta                          <  2:127966/141‑1 (MQ=255)
                                                                                                                  ttGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATc                        >  1:343820/1‑141 (MQ=255)
                                                                                                                   tGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCa                       <  1:1055716/141‑1 (MQ=255)
                                                                                                                   tGACTTATGGTTGTATCGGAGAACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCa                       <  1:769337/141‑1 (MQ=255)
                                                                                                                                tATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTggagg          >  2:118169/1‑141 (MQ=255)
                                                                                                                                tATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTggagg          >  2:118091/1‑141 (MQ=255)
                                                                                                                                 aTCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTggagga         >  1:434027/1‑141 (MQ=255)
                                                                                                                                   cGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGc       <  2:307441/141‑1 (MQ=255)
                                                                                                                                   cGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGc       <  2:430646/141‑1 (MQ=255)
                                                                                                                                    ggAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCg      <  2:830022/141‑1 (MQ=255)
                                                                                                                                     gAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCGa     >  2:95266/1‑141 (MQ=255)
                                                                                                                                      aGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCGAt    >  2:137814/1‑141 (MQ=255)
                                                                                                                                       gTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCGAtt   >  2:946498/1‑141 (MQ=255)
                                                                                                                                        tACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCGAttt  <  1:18990/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                             
TTATTGCAGGCAATGTCTACATTTTCAGATACTATGGACTGCAAAGTTGTTGGTATTTCAATGTCTAAACTTGGACTAATAAGTAGAACGGCTCAAGGCGTTTTTGGTGGTGCGTTGACTTATGGTTGTATCGGAGTACCACAAGCTCCAGGACAGATTGATGTTACTGATTTAAAAGCACAAGTGACTTTATACTAATAAAATGAATTAGGTTGCTTTTGTGTAAGTGCTGTCTTATAATTGAGAAGTATTATCATTGTTCGTGGAGGAGCGATTT  >  CP000730/888198‑888474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: