BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	914,471	G→A	5.1%	E161E (GAG→GAA)	sufS →	selenocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	914,471	0	G	A	5.1%	302.4 / 3.8	98	E161E (GAG→GAA)	sufS	selenocysteine lyase
Reads supporting (aligned to +/- strand): ref base G (52/41); new base A (2/3); total (54/44)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

ATGTTGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAGTGTTGATGGG  >  CP000730/914332‑914609

aTGTTGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAgg                                                                                                                                           >  2:978449/1‑141 (MQ=255)

 tGTTGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGa                                                                                                                                          >  1:37630/1‑141 (MQ=255)

   ttGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTCAACATCGAGGata                                                                                                                                        <  2:496181/141‑1 (MQ=255)

   ttGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGata                                                                                                                                        >  1:679895/1‑141 (MQ=255)

   ttGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACACCTGACGGTGAATTAAACATCGAGGata                                                                                                                                        >  2:411314/1‑141 (MQ=255)

      aaGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTa                                                                                                                                     <  1:614907/141‑1 (MQ=255)

       agagGGCGATGAAATTGTTGTCACTGAAATGGAGCATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAAGATATTaa                                                                                                                                    >  2:174808/1‑141 (MQ=255)

       agagGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTat                                                                                                                                    >  2:527884/1‑140 (MQ=255)

       agagGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTaa                                                                                                                                    >  2:1022815/1‑141 (MQ=255)

       agagGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTaa                                                                                                                                    <  2:160828/141‑1 (MQ=255)

          gggCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCa                                                                                                                                 <  2:880636/141‑1 (MQ=255)

           ggCGATGAAAATGTTGTCACTGACATTGAACATCATGCCAATATTGTTCCTTGGCAACAGATAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCaa                                                                                                                                <  2:486813/141‑1 (MQ=255)

               aTGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGa                                                                                                                            <  1:579010/141‑1 (MQ=255)

                  aaaTTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTa                                                                                                                         <  1:173064/141‑1 (MQ=255)

                    aTTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGTAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAAt                                                                                                                       >  2:197571/1‑141 (MQ=255)

                    aTTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTact                                                                                                                       >  1:546352/1‑139 (MQ=255)

                     ttgttgTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATg                                                                                                                      >  2:35607/1‑141 (MQ=255)

                       gttgtCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGAt                                                                                                                    >  2:291328/1‑141 (MQ=255)

                       gttgtCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGAt                                                                                                                    >  1:131280/1‑141 (MQ=255)

                       gttgtCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGAt                                                                                                                    >  1:427524/1‑141 (MQ=255)

                        ttgtCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATa                                                                                                                   <  1:93790/141‑1 (MQ=255)

                        ttgtCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATa                                                                                                                   >  2:988154/1‑141 (MQ=255)

                            cACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAAc                                                                                                               >  2:834504/1‑141 (MQ=255)

                             aCTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACa                                                                                                              <  2:37408/141‑1 (MQ=255)

                              cTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACaa                                                                                                             <  1:1079607/141‑1 (MQ=255)

                                 aaaTGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGa                                                                                                          <  2:362645/141‑1 (MQ=255)

                                 aaaTGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGa                                                                                                          <  1:978449/141‑1 (MQ=255)

                                    tGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAACACAAAGATCg                                                                                                       >  2:937542/1‑141 (MQ=255)

                                     ggAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGt                                                                                                      >  1:82177/1‑141 (MQ=255)

                                     ggAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGt                                                                                                      <  1:876195/141‑1 (MQ=255)

                                      gAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGtt                                                                                                     <  2:36753/141‑1 (MQ=255)

                                      gAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGtt                                                                                                     >  1:924795/1‑141 (MQ=255)

                                       agcatcatGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAAGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTg                                                                                                    >  2:899666/3‑141 (MQ=255)

                                        aCATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGc                                                                                                   >  2:579074/1‑141 (MQ=255)

                                          atcatGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTa                                                                                                 >  1:398397/1‑141 (MQ=255)

                                           tcatGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTAt                                                                                                <  1:413618/141‑1 (MQ=255)

                                             atGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTg                                                                                              >  2:577644/1‑141 (MQ=255)

                                                ccaaggaacaatattGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATAGTAGCTACTGaac                                                                                           >  2:362174/14‑141 (MQ=255)

                                                ccAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTGCTACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGcac                                                                                           >  1:566508/1‑141 (MQ=255)

                                                    ggaacaatattGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACatat                                                                                       >  2:955388/10‑141 (MQ=255)

                                                    ggaacaatattGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACatat                                                                                       >  2:189322/10‑141 (MQ=255)

                                                     atTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACatata                                                                                      >  1:124349/1‑141 (MQ=255)

                                                         ttCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGGGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTa                                                                                  >  1:125588/1‑141 (MQ=255)

                                                          tCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACattagatata                                                                                 <  2:468930/141‑9 (MQ=255)

                                                           ccTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGTACattagatatat                                                                                <  2:997701/141‑10 (MQ=255)

                                                           ccTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAAt                                                                                >  2:887673/1‑141 (MQ=255)

                                                            cTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAAtg                                                                               <  2:222802/141‑1 (MQ=255)

                                                            cTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAAtg                                                                               <  2:222937/141‑1 (MQ=255)

                                                               ggCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAAGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATTTCTAAtgtac                                                                            <  1:934332/141‑3 (MQ=255)

                                                               ggCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAAGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATTTCTAAtgtac                                                                            <  1:18497/141‑3 (MQ=255)

                                                                  aaCAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCg                                                                         >  1:865226/1‑141 (MQ=255)

                                                                  aaCAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCg                                                                         >  1:951221/1‑141 (MQ=255)

                                                                   aCAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCgg                                                                        <  2:563854/141‑1 (MQ=255)

                                                                    cAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGt                                                                       >  2:738136/1‑141 (MQ=255)

                                                                     aGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTa                                                                      >  1:936947/1‑141 (MQ=255)

                                                                      gTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTAc                                                                     >  2:983631/1‑141 (MQ=255)

                                                                      gTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTAc                                                                     >  2:998453/1‑141 (MQ=255)

                                                                       ttAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACa                                                                    <  1:1079191/141‑1 (MQ=255)

                                                                        tAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACaa                                                                   <  2:37630/141‑1 (MQ=255)

                                                                         aGCAAAGCGTAAAAATGCGCCATTGAAATCTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAAt                                                                  >  2:1043373/1‑141 (MQ=255)

                                                                           cAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTa                                                                >  1:118546/1‑141 (MQ=255)

                                                                               gCGTAAAAATGCGACATNNAAATTTATACCAATNACANCTGACGGTGAATTAAACATCGAGGATATTAAGGAAACGATGAATTATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAatga                                                            >  1:1029680/1‑141 (MQ=255)

                                                                                cGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAatgat                                                           >  2:149556/1‑141 (MQ=255)

                                                                                cGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAatgat                                                           >  1:398942/1‑141 (MQ=255)

                                                                                    aaaaTGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTTCACATATATCTAATGTGCTCGGTACAATTAATGATGTTa                                                       >  1:51808/1‑141 (MQ=255)

                                                                                    aaaaTGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTa                                                       <  1:752945/141‑1 (MQ=255)

                                                                                         gCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAAcc                                                  >  1:736300/1‑141 (MQ=255)

                                                                                          cGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCa                                                 <  2:52200/141‑1 (MQ=255)

                                                                                          cGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCa                                                 <  1:171841/141‑1 (MQ=255)

                                                                                          cGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCa                                                 <  2:118546/141‑1 (MQ=255)

                                                                                             cATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTg                                              <  1:91152/141‑1 (MQ=255)

                                                                                             cATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTg                                              >  1:196404/1‑141 (MQ=255)

                                                                                              aTTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGc                                             <  1:636362/141‑1 (MQ=255)

                                                                                                   aaTTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGaaa                                        <  1:688947/141‑1 (MQ=255)

                                                                                                    aTTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAAt                                       >  1:160938/1‑141 (MQ=255)

                                                                                                        ataCCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCt                                   >  1:518655/1‑141 (MQ=255)

                                                                                                         taCCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCtc                                  <  2:234074/141‑1 (MQ=255)

                                                                                                              aTGACAGCTGACGGTGAATTAAACATCGAGGATCTTAAGCCAACGATTAATGATAAAACAAAGATCGTTGCTATTGCCCATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCaa                             >  2:327725/1‑141 (MQ=255)

                                                                                                                gACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACa                           <  2:412890/141‑1 (MQ=255)

                                                                                                                    gCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCGCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGt                       <  1:565909/141‑1 (MQ=255)

                                                                                                                     cTGACGGTGAATTAATCATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTg                      <  1:901196/141‑1 (MQ=255)

                                                                                                                     cTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTg                      >  2:446038/1‑141 (MQ=255)

                                                                                                                     cTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTg                      >  1:121924/1‑141 (MQ=255)

                                                                                                                      tcaCGGTGAATTAAACATCGAGGATATTAGCCGAACGCTTACTGATAAAACAAAGCTCGTTGCTATTGCCCAACTAACTAATTTGCTCGGTACAATTAATGATGTTAAAACCATTGCTGAAATAGCTCATCCACATGGTGc                     >  2:557748/3‑141 (MQ=255)

                                                                                                                      tGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGc                     >  1:1076567/1‑141 (MQ=255)

                                                                                                                       gACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCa                    <  1:143913/141‑1 (MQ=255)

                                                                                                                         cgttGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTGGGTAGAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAAt                  <  2:1033049/138‑1 (MQ=255)

                                                                                                                           gTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTa                <  2:414528/141‑1 (MQ=255)

                                                                                                                              aaTTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCa             <  2:606656/141‑1 (MQ=255)

                                                                                                                              aaTTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATACATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCa             <  1:917663/141‑1 (MQ=255)

                                                                                                                               aTTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAg            >  2:921159/1‑141 (MQ=255)

                                                                                                                                ttAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAgt           <  1:1036546/141‑1 (MQ=255)

                                                                                                                                 tAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAgtg          <  1:223881/141‑1 (MQ=255)

                                                                                                                                  aaaCATCGAGGATATGAAGCAAACGATTAATGAGAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAgtgt         <  2:534074/141‑1 (MQ=255)

                                                                                                                                   aaCATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAGTGtt        >  2:612252/1‑141 (MQ=255)

                                                                                                                                    aCATCGAAGATATTACGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATTTCTAATGTACTCGGTACAATTAATGATGTTAAAACCATTGCAGTAATAGCTCATCAACATGGCGCAATTATCAGTGTTg       <  1:899666/141‑1 (MQ=255)

                                                                                                                                     cATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAGTGTTGa      >  2:880592/1‑141 (MQ=255)

                                                                                                                                       tCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAGTGTTGATg    >  2:497403/1‑141 (MQ=255)

                                                                                                                                         gAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAGTGTTGATggg  >  2:110814/1‑141 (MQ=255)

ATGTTGAAGAGGGCGATGAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCAAAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATCGAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCTAATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGTGCAATTATCAGTGTTGATGGG  >  CP000730/914332‑914609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`