Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 917,327 T→G 6.4% intergenic (+86/‑104) sufB → / → USA300HOU_0876 ABC superfamily ATP binding cassette transporter, membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730917,3270TG 6.4% 297.4 / 8.2 102intergenic (+86/‑104)sufB/USA300HOU_0876ABC superfamily ATP binding cassette transporter, membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (52/43);  new base G (2/5);  total (54/48)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAATACAACATGATT  >  CP000730/917187‑917467
                                                                                                                                              |                                                                                                                                            
cgcgGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAt                                                                                                                                              >  1:674704/1‑141 (MQ=255)
cgcgGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAt                                                                                                                                              >  1:275518/1‑141 (MQ=255)
cgcgGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAt                                                                                                                                              >  2:16815/1‑141 (MQ=255)
cgcgGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAt                                                                                                                                              >  2:472310/1‑141 (MQ=255)
cgcgGTCGAAATGAACCGTTTAATCAAGTCCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGAGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAt                                                                                                                                              >  1:524277/1‑141 (MQ=255)
     tCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACtg                                                                                                                                         <  1:519331/141‑1 (MQ=255)
        aaaTGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAGTAATGTgg                                                                                                                                      <  2:882670/141‑1 (MQ=255)
         aaTGAACGGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGc                                                                                                                                     >  1:859681/1‑141 (MQ=255)
         aaTGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAGTAATGTGGc                                                                                                                                     <  1:492805/141‑1 (MQ=255)
          aTGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCt                                                                                                                                    <  1:795829/141‑1 (MQ=255)
          aTGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCt                                                                                                                                    <  2:580197/141‑1 (MQ=255)
            gAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCttt                                                                                                                                  >  2:1051410/1‑141 (MQ=255)
             aaCCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCtttt                                                                                                                                 <  1:170741/141‑1 (MQ=255)
             aaCCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCtttt                                                                                                                                 >  2:42302/1‑141 (MQ=255)
              aCCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCttttt                                                                                                                                <  1:343918/141‑1 (MQ=255)
              aCCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCttttt                                                                                                                                <  1:368557/141‑1 (MQ=255)
              aCCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACAGTAATGTGGCttttt                                                                                                                                <  2:411400/141‑1 (MQ=255)
                cGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCttttttt                                                                                                                              <  1:742494/141‑1 (MQ=255)
                 gTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAACAAAAAAAGCCACAGTAATGTGGCtttttg                                                                                                                               <  2:451556/141‑2 (MQ=255)
                  tttAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGt                                                                                                                            >  2:374009/1‑141 (MQ=255)
                  tttAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATAGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGt                                                                                                                            >  2:722256/1‑141 (MQ=255)
                      aTCAAGTTCGAAATGGAAGGTAGTCTTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGCCTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTta                                                                                                                        >  2:76955/1‑141 (MQ=255)
                      aTCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTta                                                                                                                        >  1:119104/1‑141 (MQ=255)
                      aTCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTta                                                                                                                        >  1:198583/1‑141 (MQ=255)
                       tCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTtat                                                                                                                       >  2:363560/1‑141 (MQ=255)
                         aaGTTCGAAATGGAAGGTAGTCTTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATATCATTTTGATACTGAATATAAC‑‑AAAAATCAACATTACTATGGCTTTTTTTGTTTTATaa                                                                                                                     >  2:554695/1‑141 (MQ=255)
                              cGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGGAAATGGGTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTaaa                                                                                                                >  1:238257/1‑141 (MQ=255)
                              cGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTaaa                                                                                                                <  2:63659/141‑1 (MQ=255)
                              cGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTaaa                                                                                                                <  1:408715/141‑1 (MQ=255)
                               gAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAAt                                                                                                               >  1:1000319/1‑141 (MQ=255)
                               gAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAAt                                                                                                               <  1:129371/141‑1 (MQ=255)
                               gAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAAt                                                                                                               <  1:1040612/141‑1 (MQ=255)
                                aaaTGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATc                                                                                                              >  1:944407/1‑141 (MQ=255)
                                aaaTGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATc                                                                                                              >  1:538518/1‑141 (MQ=255)
                                aaaTGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATc                                                                                                              >  2:271443/1‑141 (MQ=255)
                                  aTGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCgg                                                                                                            <  1:176139/141‑1 (MQ=255)
                                          tagtaTTGGATAGCTGTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATGGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGGGGCTTGTTTTGTTTTATAACTAAATCGGATTgatag                                                                                                    <  2:429172/141‑1 (MQ=255)
                                          aagtaTTGGATAGCTTTACACCGCGTAGTTAAGCCATTCTCGACTTCCGAAAATGGCTATTGCTACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGAGGCTTATTTTGTTTTATAACTAAATCAGATGgatag                                                                                                    >  2:1060996/2‑141 (MQ=255)
                                             taTTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTgatagata                                                                                                 >  1:399542/1‑141 (MQ=255)
                                              aTTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGCTACTGAATATAAC‑‑AAAAAGCCACAGTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATaa                                                                                                >  1:318126/1‑141 (MQ=255)
                                              aTTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATaa                                                                                                >  1:396844/1‑141 (MQ=255)
                                              aTTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATaa                                                                                                >  1:993328/1‑141 (MQ=255)
                                                      gCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTa                                                                                        <  1:450250/141‑1 (MQ=255)
                                                        tttAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACt                                                                                      <  2:1025661/141‑1 (MQ=255)
                                                        tttAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACt                                                                                      <  2:789448/141‑1 (MQ=255)
                                                                gcgTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTtata                                                                              >  1:324335/1‑141 (MQ=255)
                                                                 cgTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGGATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTatat                                                                             >  2:207575/1‑141 (MQ=255)
                                                                 cgTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTatat                                                                             >  2:892982/1‑141 (MQ=255)
                                                                  gttgttAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATc                                                                            >  1:809309/1‑141 (MQ=255)
                                                                  gttgttAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATc                                                                            >  2:40489/1‑141 (MQ=255)
                                                                    tgttAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAg                                                                          <  2:183004/141‑1 (MQ=255)
                                                                     gttAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGt                                                                         >  1:561133/1‑141 (MQ=255)
                                                                       tAAGCCATTCTTGACTTCCGGAAATGGGTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGtcc                                                                       >  1:5511/1‑139 (MQ=255)
                                                                        aaGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCg                                                                      <  1:392994/141‑1 (MQ=255)
                                                                        aaGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCg                                                                      <  1:51710/141‑1 (MQ=255)
                                                                          gCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGAt                                                                    <  2:668371/141‑1 (MQ=255)
                                                                           ccATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGAtt                                                                   >  2:964080/1‑141 (MQ=255)
                                                                             aTTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGAtttt                                                                 <  1:396112/141‑1 (MQ=255)
                                                                              ttCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGAttttt                                                                <  1:369823/141‑1 (MQ=255)
                                                                               tCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGAtttttt                                                               >  2:525638/1‑141 (MQ=255)
                                                                                 ttGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGa                                                             >  1:331154/1‑141 (MQ=255)
                                                                                 tcggaagtcaagaaTGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGa                                                             >  1:38336/13‑141 (MQ=255)
                                                                                  tGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGAt                                                            >  1:42709/1‑141 (MQ=255)
                                                                                   gACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGAtt                                                           >  2:1007106/1‑141 (MQ=255)
                                                                                      ttCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGgt                                                        >  1:167916/1‑141 (MQ=255)
                                                                                         cGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTa                                                     <  1:619704/141‑1 (MQ=255)
                                                                                            aaaTGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTaaaa                                                  <  2:621502/141‑1 (MQ=255)
                                                                                             aaTGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTaaaaa                                                 >  1:484662/1‑141 (MQ=255)
                                                                                               tGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTAGATTTTTTGATTGGTGTaaaaaaa                                               >  1:394652/1‑140 (MQ=255)
                                                                                                ggCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAaata                                              >  1:438097/1‑141 (MQ=255)
                                                                                                 gtatcaatagccATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACCGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAaataa                                             >  2:968004/11‑141 (MQ=255)
                                                                                                 gCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAaataa                                             >  2:991191/1‑141 (MQ=255)
                                                                                                   tATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATc                                           <  1:38594/141‑1 (MQ=255)
                                                                                                    aTTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATct                                          >  1:437028/1‑140 (MQ=255)
                                                                                                     ttGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCAt                                         >  1:774379/1‑141 (MQ=255)
                                                                                                      tGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCAtt                                        <  2:525615/141‑1 (MQ=255)
                                                                                                      tGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCAtt                                        <  1:362873/141‑1 (MQ=255)
                                                                                                      tGATACCATTTTGATACTGAATATAAA‑‑AAAAAGCCCCATTACGGTGGTTTTTTTTGTTTTATAACTAAATGGGTTTGGTAGATAAGTTTTTTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATAAtt                                        <  2:788498/141‑1 (MQ=255)
                                                                                                        aTACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGa                                      <  1:370594/141‑1 (MQ=255)
                                                                                                           ccATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGAtgg                                   <  2:851112/141‑1 (MQ=255)
                                                                                                            cATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTCGATTGGTGTAAAAAATAATCATTGAtggt                                  <  2:142768/141‑1 (MQ=255)
                                                                                                              ttttGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGAtggtgg                                >  2:236879/1‑141 (MQ=255)
                                                                                                              ttttGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGAtggtgg                                <  1:16815/141‑1 (MQ=255)
                                                                                                               tttGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGa                               <  1:271443/141‑1 (MQ=255)
                                                                                                               tttGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGa                               <  1:1051410/141‑1 (MQ=255)
                                                                                                                 tGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATa                             <  1:1020614/141‑1 (MQ=255)
                                                                                                                 tGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATa                             >  2:339016/1‑141 (MQ=255)
                                                                                                                   aTACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATaaa                           <  1:413930/141‑1 (MQ=255)
                                                                                                                      cTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCg                        <  2:331663/141‑1 (MQ=255)
                                                                                                                      cTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCg                        >  2:148939/1‑141 (MQ=255)
                                                                                                                       tGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGa                       <  1:148121/141‑1 (MQ=255)
                                                                                                                            ataAC‑‑AAAAAGCCACATTACTGTGGCATTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGacaaca                  <  2:859681/141‑1 (MQ=255)
                                                                                                                                 aaaaaaaGCCACAGTAATGTGGC‑‑TTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAAt             >  1:1079366/3‑141 (MQ=255)
                                                                                                                                      aaGCCACATTACTGTGGCTTTTTTAGTTTAATAACTAAATCGGATTGATAGATAAGCCTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGGAAAAAATAATCATAGATGGTGGATAAAGCGACAACACAAATaca          >  2:876152/1‑141 (MQ=255)
                                                                                                                                       aGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCCATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAATacaa         >  1:565006/1‑141 (MQ=255)
                                                                                                                                         ccACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATCGTGGATAAAGCGACAACACACATaaaaca       >  1:541461/1‑141 (MQ=255)
                                                                                                                                         ccACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTAATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACCACTacaacc       >  2:1024021/1‑140 (MQ=255)
                                                                                                                                            caTTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAATACAACATGa    >  1:497411/1‑141 (MQ=255)
                                                                                                                                             aTTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAATACAACATGAt   >  1:307912/1‑141 (MQ=255)
                                                                                                                                              ttACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAATACAACATGAtt  <  2:658809/141‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
CGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAAC‑‑AAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTACTTATTTATATCAGTTCGATTTTTTGATTGGTGTAAAAAATAATCATTGATGGTGGATAAAGCGACAACACAAATACAACATGATT  >  CP000730/917187‑917467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: