Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 922,773 G→A 5.9% E159K (GAA→AAA)  yunD → bifunctional 5‑nucleotidase/2,3‑cyclic phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730922,7730GA 5.9% 317.0 / 5.1 101E159K (GAA→AAA) yunDbifunctional 5‑nucleotidase/2,3‑cyclic phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (43/52);  new base A (3/3);  total (46/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 103 total aligned reads displayed.

TAATATCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAA  >  CP000730/922634‑922913
                                                                                                                                           |                                                                                                                                            
taataTCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGa                                                                                                                                             <  1:99907/141‑1 (MQ=255)
taataTCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGa                                                                                                                                             <  2:832345/141‑1 (MQ=255)
taataTCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGa                                                                                                                                             <  1:99884/141‑1 (MQ=255)
 aataTCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCAGTTTATCGTGCAGTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGaa                                                                                                                                            <  2:432526/141‑1 (MQ=255)
  atatCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACATCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAAc                                                                                                                                           <  2:843339/141‑1 (MQ=255)
    atCGTTTCTTCTTATATTAAGGACATAGAGGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACtt                                                                                                                                         >  2:310554/1‑141 (MQ=255)
     tCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTc                                                                                                                                        >  2:583638/1‑141 (MQ=255)
     tCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGAATAGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTc                                                                                                                                        >  1:10321/1‑141 (MQ=255)
       gTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCaa                                                                                                                                      >  1:144556/1‑141 (MQ=255)
       gTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCaa                                                                                                                                      >  2:964362/1‑141 (MQ=255)
            ttcttATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAgg                                                                                                                                 <  1:908132/141‑1 (MQ=255)
            ttcttATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAgg                                                                                                                                 <  2:870923/141‑1 (MQ=255)
            ttcttATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAgg                                                                                                                                 <  2:289609/141‑1 (MQ=255)
             tcttATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGt                                                                                                                                <  2:339763/141‑1 (MQ=255)
             tcttATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGt                                                                                                                                <  1:49396/141‑1 (MQ=255)
             tcttATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGt                                                                                                                                <  1:49142/141‑1 (MQ=255)
                tataTTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTaaa                                                                                                                             >  1:818506/1‑141 (MQ=255)
                 atatTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAAt                                                                                                                            >  1:1082466/1‑141 (MQ=255)
                        ggACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTtgttgt                                                                                                                     >  2:558735/1‑141 (MQ=255)
                        ggACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGt                                                                                                                     >  2:202772/1‑141 (MQ=255)
                         gACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTa                                                                                                                    <  2:932467/141‑1 (MQ=255)
                           cATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTAtt                                                                                                                  <  2:703012/141‑1 (MQ=255)
                            aTAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTa                                                                                                                 >  2:32212/1‑141 (MQ=255)
                                 cGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGt                                                                                                            >  2:367132/1‑141 (MQ=255)
                                   gtgtGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGc                                                                                                          >  1:444594/1‑141 (MQ=255)
                                    tgtgAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCt                                                                                                         >  2:760585/1‑141 (MQ=255)
                                     gtgAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTa                                                                                                        >  2:174950/1‑141 (MQ=255)
                                     gtgAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTa                                                                                                        <  1:70186/141‑1 (MQ=255)
                                           aTACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCAt                                                                                                  <  1:277100/141‑1 (MQ=255)
                                                aTTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTgg                                                                                             <  1:969876/141‑1 (MQ=255)
                                                 ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                            <  2:131222/141‑1 (MQ=255)
                                                 ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                            >  2:748499/1‑141 (MQ=255)
                                                 ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                            >  1:731334/1‑141 (MQ=255)
                                                 ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                            <  1:734139/141‑1 (MQ=255)
                                                  tCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCa                                                                                           >  2:963989/1‑141 (MQ=255)
                                                   cGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAt                                                                                          <  1:215160/141‑1 (MQ=255)
                                                    gTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAGCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAtt                                                                                         <  2:621339/141‑1 (MQ=255)
                                                     ttGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAttt                                                                                        >  2:404613/1‑141 (MQ=255)
                                                     ttGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAttt                                                                                        >  2:218510/1‑141 (MQ=255)
                                                       gCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTttc                                                                                      <  2:80676/141‑1 (MQ=255)
                                                        cAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTttct                                                                                     <  2:957309/141‑1 (MQ=255)
                                                        cAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTttct                                                                                     <  2:652180/141‑1 (MQ=255)
                                                              cAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATg                                                                               <  1:444153/141‑1 (MQ=255)
                                                              cAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATg                                                                               <  1:444168/141‑1 (MQ=255)
                                                                  aCCTTTTACCCCATTTTATCGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGACATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAAc                                                                           >  2:795401/1‑141 (MQ=255)
                                                                  aCCTTTTACCCCATTTTATCGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAAc                                                                           <  2:896869/141‑1 (MQ=255)
                                                                  aCCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAAc                                                                           >  1:368830/1‑141 (MQ=255)
                                                                   ccTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACa                                                                          <  1:772879/141‑1 (MQ=255)
                                                                    cTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACAt                                                                         >  1:357150/1‑141 (MQ=255)
                                                                     ttttACCCCATTTTATCGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACAtt                                                                        >  1:210137/1‑141 (MQ=255)
                                                                      tttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTa                                                                       >  1:309235/1‑141 (MQ=255)
                                                                      tttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTa                                                                       >  2:645334/1‑141 (MQ=255)
                                                                      tttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACGTCAACGAGGTAAATTTGATGTATTCATCGTGTTAAGTCATTGTGGCATTTTCTTCGATGAAACATTa                                                                       <  1:935239/141‑1 (MQ=255)
                                                                       ttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTAt                                                                      <  2:586664/141‑1 (MQ=255)
                                                                         aCCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGc                                                                    >  2:395749/1‑141 (MQ=255)
                                                                         aCCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGc                                                                    >  2:192483/1‑141 (MQ=255)
                                                                           cccATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCa                                                                  <  1:940991/141‑1 (MQ=255)
                                                                             cATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATTAAACATTATGCCAAg                                                                <  2:22021/141‑1 (MQ=255)
                                                                                   aTCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGc                                                          <  2:218986/141‑1 (MQ=255)
                                                                                   aTCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGc                                                          <  1:47114/141‑1 (MQ=255)
                                                                                   aTCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGc                                                          <  1:943093/141‑1 (MQ=255)
                                                                                    tCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGcc                                                         >  1:859488/1‑141 (MQ=255)
                                                                                     cGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCt                                                        <  2:601760/141‑1 (MQ=255)
                                                                                      gTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTg                                                       >  1:317740/1‑141 (MQ=255)
                                                                                               aTTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATg                                              >  1:476342/1‑141 (MQ=255)
                                                                                                ttGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGt                                             <  2:609088/141‑1 (MQ=255)
                                                                                                ttGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGt                                             <  2:2373/141‑1 (MQ=255)
                                                                                                 tGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTc                                            >  2:165305/1‑141 (MQ=255)
                                                                                                     ttGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCAttt                                        >  1:133935/1‑141 (MQ=255)
                                                                                                         tACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAGTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTgg                                    <  1:141611/141‑1 (MQ=255)
                                                                                                         tACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTgg                                    >  2:462715/1‑141 (MQ=255)
                                                                                                           ccGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTa                                  >  2:971138/1‑141 (MQ=255)
                                                                                                           ccGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTa                                  >  2:264587/1‑141 (MQ=255)
                                                                                                           ccGAACCACTAGAATCTATAAAAGAAGAACTTGACCTTCAACGAGGAAAATTTGAAGTATTAATCGTGCTAAGACATTGTGGCATTTTCTTCGATGAAACCTTATGCCAAGAATTGTATGAAATTGATGACATTATTGGTa                                  >  2:971025/1‑141 (MQ=255)
                                                                                                                 cACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATa                            <  2:117657/141‑1 (MQ=255)
                                                                                                                  aCTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATAc                           <  2:167063/141‑1 (MQ=255)
                                                                                                                   cTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACg                          >  2:514930/1‑141 (MQ=255)
                                                                                                                    ttGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGc                         <  2:920825/141‑1 (MQ=255)
                                                                                                                    ttGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGc                         >  1:127955/1‑141 (MQ=255)
                                                                                                                     tGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGca                        <  1:450703/141‑1 (MQ=255)
                                                                                                                     tGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGca                        <  1:456339/141‑1 (MQ=255)
                                                                                                                        aTCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGcatca                     >  1:448028/1‑141 (MQ=255)
                                                                                                                        aTCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGcatca                     >  1:288237/1‑141 (MQ=255)
                                                                                                                        aTCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGcatca                     <  1:26832/141‑1 (MQ=255)
                                                                                                                          cTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCatt                   >  1:258815/1‑141 (MQ=255)
                                                                                                                          cTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCatt                   >  1:380536/1‑141 (MQ=255)
                                                                                                                              aaaaGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAttt               <  1:763981/141‑1 (MQ=255)
                                                                                                                              aaaaGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAttt               <  1:367132/141‑1 (MQ=255)
                                                                                                                               aaaGAAGAAATTGAACTTCAACGAGGTACATTTGATGTCTTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAtttt              >  1:846140/1‑141 (MQ=255)
                                                                                                                               aaaGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAtttt              <  2:818506/141‑1 (MQ=255)
                                                                                                                                aagaagAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTg             <  1:936579/141‑1 (MQ=255)
                                                                                                                                     gaaATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACAt        >  1:364453/1‑141 (MQ=255)
                                                                                                                                     gaaATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCACCATTATTTTGAACAt        >  2:956204/1‑141 (MQ=255)
                                                                                                                                       aaTTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACCTTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGACCATgg      <  2:153921/141‑1 (MQ=255)
                                                                                                                                       aaTTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATgg      <  1:501578/141‑1 (MQ=255)
                                                                                                                                        aTTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGt     <  2:68034/141‑1 (MQ=255)
                                                                                                                                         ttGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTg    <  2:344251/141‑1 (MQ=255)
                                                                                                                                          tGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATCGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGa   <  1:202772/141‑1 (MQ=255)
                                                                                                                                           gAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaa  >  1:695952/1‑141 (MQ=255)
                                                                                                                                           gAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaa  <  1:645334/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAATATCGTTTCTTCTTATATTAAGGACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAA  >  CP000730/922634‑922913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: