Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 922,800 T→A 6.0% L168I (TTA→ATA)  yunD → bifunctional 5‑nucleotidase/2,3‑cyclic phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730922,8000TA 6.0% 310.1 / 7.2 99L168I (TTA→ATA) yunDbifunctional 5‑nucleotidase/2,3‑cyclic phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (46/47);  new base A (3/3);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 102 total aligned reads displayed.

CATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACTGATGGCGGC  >  CP000730/922661‑922939
                                                                                                                                           |                                                                                                                                           
cATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTAtt                                                                                                                                            <  2:703012/141‑1 (MQ=255)
cATAGACGGTGTGAAAATACTATCCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTAtt                                                                                                                                            <  2:353983/141‑1 (MQ=255)
 aTAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTa                                                                                                                                           >  2:32212/1‑141 (MQ=255)
      cGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGt                                                                                                                                      >  2:367132/1‑141 (MQ=255)
        gtgtGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGc                                                                                                                                    >  1:444594/1‑141 (MQ=255)
         tgtgAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCt                                                                                                                                   >  2:760585/1‑141 (MQ=255)
          gtgAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTa                                                                                                                                  >  2:174950/1‑141 (MQ=255)
          gtgAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTa                                                                                                                                  <  1:70186/141‑1 (MQ=255)
                aTACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCAt                                                                                                                            <  1:277100/141‑1 (MQ=255)
                     aTTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTgg                                                                                                                       <  1:969876/141‑1 (MQ=255)
                      ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                                                      >  1:731334/1‑141 (MQ=255)
                      ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                                                      <  2:131222/141‑1 (MQ=255)
                      ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                                                      >  2:748499/1‑141 (MQ=255)
                      ttCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGc                                                                                                                      <  1:734139/141‑1 (MQ=255)
                       tCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGTATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCATCGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCa                                                                                                                     <  1:725965/141‑1 (MQ=255)
                       tCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCa                                                                                                                     >  2:963989/1‑141 (MQ=255)
                        cGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAt                                                                                                                    <  1:215160/141‑1 (MQ=255)
                         gTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAGCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAtt                                                                                                                   <  2:621339/141‑1 (MQ=255)
                          ttGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAttt                                                                                                                  >  2:404613/1‑141 (MQ=255)
                          ttGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAttt                                                                                                                  >  2:218510/1‑141 (MQ=255)
                            gCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTttc                                                                                                                <  2:80676/141‑1 (MQ=255)
                             cAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTttct                                                                                                               <  2:957309/141‑1 (MQ=255)
                             cAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTttct                                                                                                               <  2:652180/141‑1 (MQ=255)
                                   cAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATg                                                                                                         <  1:444168/141‑1 (MQ=255)
                                   cAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATg                                                                                                         <  1:444153/141‑1 (MQ=255)
                                       aCCTTTTACCCCATTTTATCGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGACATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAAc                                                                                                     >  2:795401/1‑141 (MQ=255)
                                       aCCTTTTACCCCATTTTATCGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAAc                                                                                                     <  2:896869/141‑1 (MQ=255)
                                       aCCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAAc                                                                                                     >  1:368830/1‑141 (MQ=255)
                                        ccTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACa                                                                                                    <  1:772879/141‑1 (MQ=255)
                                         cTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACAt                                                                                                   >  1:357150/1‑141 (MQ=255)
                                          ttttACCCCATTTTATCGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACAtt                                                                                                  >  1:210137/1‑141 (MQ=255)
                                           tttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTa                                                                                                 >  2:645334/1‑141 (MQ=255)
                                           tttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTa                                                                                                 >  1:309235/1‑141 (MQ=255)
                                           tttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACGTCAACGAGGTAAATTTGATGTATTCATCGTGTTAAGTCATTGTGGCATTTTCTTCGATGAAACATTa                                                                                                 <  1:935239/141‑1 (MQ=255)
                                            ttACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTAt                                                                                                <  2:586664/141‑1 (MQ=255)
                                              aCCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGc                                                                                              >  2:192483/1‑141 (MQ=255)
                                              aCCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGc                                                                                              >  2:395749/1‑141 (MQ=255)
                                                cccATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCa                                                                                            <  1:940991/141‑1 (MQ=255)
                                                  cATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATTAAACATTATGCCAAg                                                                                          <  2:22021/141‑1 (MQ=255)
                                                        aTCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGc                                                                                    <  1:47114/141‑1 (MQ=255)
                                                        aTCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGc                                                                                    <  2:218986/141‑1 (MQ=255)
                                                         tCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGcc                                                                                   >  1:859488/1‑141 (MQ=255)
                                                          cGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCt                                                                                  <  2:601760/141‑1 (MQ=255)
                                                           gTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTg                                                                                 >  1:317740/1‑141 (MQ=255)
                                                                    aTTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATg                                                                        >  1:476342/1‑141 (MQ=255)
                                                                     ttGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGt                                                                       <  2:609088/141‑1 (MQ=255)
                                                                     ttGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGt                                                                       <  2:2373/141‑1 (MQ=255)
                                                                      tGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTc                                                                      >  2:165305/1‑141 (MQ=255)
                                                                          ttGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCAttt                                                                  >  1:133935/1‑141 (MQ=255)
                                                                              tACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCAGTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTgg                                                              <  1:141611/141‑1 (MQ=255)
                                                                              tACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTgg                                                              >  2:462715/1‑141 (MQ=255)
                                                                                ccGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTa                                                            >  2:971138/1‑141 (MQ=255)
                                                                                ccGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTa                                                            >  2:264587/1‑141 (MQ=255)
                                                                                ccGAACCACTAGAATCTATAAAAGAAGAACTTGACCTTCAACGAGGAAAATTTGAAGTATTAATCGTGCTAAGACATTGTGGCATTTTCTTCGATGAAACCTTATGCCAAGAATTGTATGAAATTGATGACATTATTGGTa                                                            >  2:971025/1‑141 (MQ=255)
                                                                                      cACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATa                                                      <  2:117657/141‑1 (MQ=255)
                                                                                       aCTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATAc                                                     <  2:167063/141‑1 (MQ=255)
                                                                                        cTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACg                                                    >  2:514930/1‑141 (MQ=255)
                                                                                         ttGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGc                                                   >  1:127955/1‑141 (MQ=255)
                                                                                         ttGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGc                                                   <  2:920825/141‑1 (MQ=255)
                                                                                          tGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGca                                                  <  1:450703/141‑1 (MQ=255)
                                                                                          tGAATCTATAAAAGAAGAAATTAAACATCAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGca                                                  <  1:456339/141‑1 (MQ=255)
                                                                                             aTCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGcatca                                               <  1:26832/141‑1 (MQ=255)
                                                                                             aTCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGcatca                                               >  1:288237/1‑141 (MQ=255)
                                                                                             aTCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGcatca                                               >  1:448028/1‑141 (MQ=255)
                                                                                               cTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCatt                                             >  1:380536/1‑141 (MQ=255)
                                                                                               cTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCatt                                             >  1:258815/1‑141 (MQ=255)
                                                                                                   aaaaGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAttt                                         <  1:763981/141‑1 (MQ=255)
                                                                                                   aaaaGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAttt                                         <  1:367132/141‑1 (MQ=255)
                                                                                                    aaaGAAGAAATTGAACTTCAACGAGGTACATTTGATGTCTTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAtttt                                        >  1:846140/1‑141 (MQ=255)
                                                                                                    aaaGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAtttt                                        <  2:818506/141‑1 (MQ=255)
                                                                                                     aagaagAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTg                                       <  1:936579/141‑1 (MQ=255)
                                                                                                          gaaATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACAt                                  >  1:364453/1‑141 (MQ=255)
                                                                                                          gaaATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCACCATTATTTTGAACAt                                  >  2:956204/1‑141 (MQ=255)
                                                                                                            aaTTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACCTTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGACCATgg                                <  2:153921/141‑1 (MQ=255)
                                                                                                            aaTTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATgg                                <  1:501578/141‑1 (MQ=255)
                                                                                                             aTTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGt                               <  2:68034/141‑1 (MQ=255)
                                                                                                              ttGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTg                              <  2:344251/141‑1 (MQ=255)
                                                                                                               tGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATCGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGa                             <  1:202772/141‑1 (MQ=255)
                                                                                                                gAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaa                            >  1:695952/1‑141 (MQ=255)
                                                                                                                gAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaa                            <  1:645334/141‑1 (MQ=255)
                                                                                                                 aaCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaaa                           <  1:548236/141‑1 (MQ=255)
                                                                                                                 aaCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaaa                           <  1:444290/141‑1 (MQ=255)
                                                                                                                 aaCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaaa                           <  1:601741/141‑1 (MQ=255)
                                                                                                                 aaCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGaaa                           >  2:464827/1‑141 (MQ=255)
                                                                                                                  aCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAAt                          >  1:82963/1‑141 (MQ=255)
                                                                                                                  aCTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAAt                          >  2:856504/1‑141 (MQ=255)
                                                                                                                    ttCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCa                        >  2:330229/1‑141 (MQ=255)
                                                                                                                     tCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCaa                       >  1:903273/1‑141 (MQ=255)
                                                                                                                      cAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCaat                      >  1:922470/1‑141 (MQ=255)
                                                                                                                      cAACGAGGGAAAGTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATCCCAAGAATAGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATTATTATTATCAACATGGAGATATCtat                      >  2:894711/1‑141 (MQ=255)
                                                                                                                            ggTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGgt                <  1:856472/141‑1 (MQ=255)
                                                                                                                             gTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGgtg               >  2:949402/1‑141 (MQ=255)
                                                                                                                             gTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGgtg               >  2:912261/1‑141 (MQ=255)
                                                                                                                                aaTTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATACTgggttac            >  1:961210/1‑136 (MQ=255)
                                                                                                                                 aTTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACt           <  1:694834/141‑1 (MQ=255)
                                                                                                                                 aTTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACt           >  1:126107/1‑141 (MQ=255)
                                                                                                                                   ttGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACTGa         <  2:896935/141‑1 (MQ=255)
                                                                                                                                      aTGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACTGATgg      <  1:583638/141‑1 (MQ=255)
                                                                                                                                          tttATTCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACTGATggcggc  <  1:817183/140‑1 (MQ=255)
                                                                                                                                          aTTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACTGATggcggc  <  2:263189/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTATCGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTTCAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAAACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTATTTTGAACATGGTGAAATCAATAATGGTGTACTGATGGCGGC  >  CP000730/922661‑922939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: