BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	950,093	T→A	6.8%	intergenic (+880/‑275)	prsA1 → / → yqiG	peptidylprolyl isomerase/flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	950,093	0	T	A	6.8%	309.5 / 9.1	99	intergenic (+880/‑275)	prsA1/yqiG	peptidylprolyl isomerase/flavin reductase
Reads supporting (aligned to +/- strand): ref base T (49/43); new base A (3/4); total (52/47)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGT  >  CP000730/949953‑950233

gAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAt                                                                                                                                                <  2:770514/141‑1 (MQ=255)

  ccGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt                                                                                                                                              >  2:520309/2‑141 (MQ=255)

  aCGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAGACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt                                                                                                                                              <  2:745321/141‑1 (MQ=255)

    gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta                                                                                                                                            >  1:143668/1‑141 (MQ=255)

    gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta                                                                                                                                            >  2:91140/1‑141 (MQ=255)

    gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta                                                                                                                                            >  2:21981/1‑141 (MQ=255)

     cTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttat                                                                                                                                           <  1:987291/141‑1 (MQ=255)

      ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                          >  1:1012550/1‑141 (MQ=255)

       tACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAGAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatta                                                                                                                                         >  1:692197/1‑141 (MQ=255)

             tCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACt                                                                                                                                   <  2:290182/141‑1 (MQ=255)

               tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTaa                                                                                                                                 <  1:439062/141‑1 (MQ=255)

               tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTaa                                                                                                                                 <  1:418807/141‑1 (MQ=255)

                  aTAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtct                                                                                                                              <  1:327005/141‑1 (MQ=255)

                   tAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtctc                                                                                                                             >  2:219861/1‑141 (MQ=255)

                    aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            <  2:390146/141‑1 (MQ=255)

                    aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            <  1:122163/141‑1 (MQ=255)

                     aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCtt                                                                                                                           <  2:433058/141‑1 (MQ=255)

                     aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTGCTTATACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCtt                                                                                                                           <  2:533323/141‑1 (MQ=38)

                      aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                          <  2:34584/141‑1 (MQ=255)

                       aCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCtttt                                                                                                                         <  2:769704/141‑1 (MQ=255)

                         attCAAAGCGAGCAATGCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                     <  2:124506/139‑1 (MQ=255)

                         aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                     >  1:656900/1‑141 (MQ=255)

                         aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                     <  1:712250/141‑1 (MQ=255)

                          gTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑Gt                                                                                                                    >  2:594774/1‑141 (MQ=255)

                           tCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTc                                                                                                                   <  1:33392/141‑1 (MQ=255)

                             aaaGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGACTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTa                                                                                                                 >  2:443723/1‑141 (MQ=255)

                                  gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa                                                                                                            >  1:287068/1‑141 (MQ=255)

                                  gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa                                                                                                            <  2:870245/141‑1 (MQ=255)

                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          >  1:303422/1‑141 (MQ=255)

                                    gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                          <  2:219773/141‑1 (MQ=255)

                                      aaTTCGAAACGACTAAAGGACCAGATAAATCGTACCGAAAAACATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                        <  1:597274/141‑1 (MQ=255)

                                      aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                        <  1:91140/141‑1 (MQ=255)

                                        ttCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTaa                                                                                                      >  2:238286/1‑141 (MQ=255)

                                         tCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAat                                                                                                     >  2:621314/1‑141 (MQ=255)

                                          cGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAata                                                                                                    >  2:878168/1‑141 (MQ=255)

                                            aaaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtg                                                                                                  <  1:104037/141‑1 (MQ=255)

                                             aaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtga                                                                                                 >  2:503699/1‑141 (MQ=255)

                                              aCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtgat                                                                                                <  1:182811/141‑1 (MQ=255)

                                               cGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtgatt                                                                                               <  2:956170/141‑1 (MQ=255)

                                                   tAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATgattgatt                                                                                           <  1:643000/141‑1 (MQ=255)

                                                    aaaGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTc                                                                                          <  1:808900/141‑1 (MQ=255)

                                                       ggAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTAt                                                                                       >  1:712956/1‑141 (MQ=255)

                                                          aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTTTACGATAACGTAATATGATTGATTCTATTTa                                                                                    >  2:44694/1‑141 (MQ=255)

                                                          aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑G‑ATCTACGATAACGTAATATGATTGATTCTATTTa                                                                                    >  2:602685/1‑141 (MQ=255)

                                                           cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                   <  1:411100/141‑1 (MQ=255)

                                                           cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                   <  1:206173/141‑1 (MQ=255)

                                                            aGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTaca                                                                                  >  2:1025903/1‑141 (MQ=255)

                                                            aGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTaca                                                                                  >  1:210534/1‑141 (MQ=255)

                                                              aTAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACg                                                                                >  1:10211/1‑141 (MQ=255)

                                                                      tACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                        >  2:1030898/1‑141 (MQ=255)

                                                                       aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAACTgg                                                                       >  2:807678/1‑141 (MQ=255)

                                                                       aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                       >  1:994858/1‑141 (MQ=255)

                                                                        ccGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGt                                                                      <  2:333389/141‑1 (MQ=255)

                                                                         cGAAAATCATACAAAGGGTGTGAAATGAAAGTTTCTTAGACTATAAAAGGGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt                                                                     <  2:935344/141‑1 (MQ=255)

                                                                           aaaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTa                                                                   >  1:301794/1‑141 (MQ=255)

                                                                            aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa                                                                  >  2:843095/1‑141 (MQ=255)

                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            <  1:845946/79‑1 (MQ=255)

                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                            >  2:845946/1‑79 (MQ=255)

                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                            >  2:1054256/1‑141 (MQ=255)

                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                            <  1:883703/141‑1 (MQ=255)

                                                                                  tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                            >  2:774569/1‑141 (MQ=255)

                                                                                   aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACa                                                           >  2:63508/1‑141 (MQ=255)

                                                                                   aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATCTACACGTACAAATGGTTTAAGGTGACa                                                           >  2:1011674/1‑141 (MQ=255)

                                                                                    cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                          >  2:560165/1‑141 (MQ=255)

                                                                                    cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                          <  2:1041038/141‑1 (MQ=255)

                                                                                       aGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATc                                                       <  2:261969/141‑1 (MQ=255)

                                                                                        gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                      <  1:519489/141‑1 (MQ=255)

                                                                                        gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                      >  1:389866/1‑141 (MQ=255)

                                                                                        gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                      <  2:484411/141‑1 (MQ=255)

                                                                                           tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTGGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                   >  1:655431/1‑141 (MQ=255)

                                                                                           tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                   >  1:571585/1‑141 (MQ=255)

                                                                                           tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                   >  2:290685/1‑141 (MQ=255)

                                                                                            cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa                                                  >  1:249847/1‑141 (MQ=255)

                                                                                            cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa                                                  >  2:626708/1‑141 (MQ=255)

                                                                                            cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa                                                  <  1:1056896/141‑1 (MQ=255)

                                                                                               aaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCt                                               >  2:824612/1‑141 (MQ=255)

                                                                                                    aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                          <  1:624837/141‑1 (MQ=255)

                                                                                                    aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                          <  1:634127/141‑1 (MQ=255)

                                                                                                       gTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTGTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtaga                                       <  1:427531/141‑1 (MQ=255)

                                                                                                        tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat                                      <  1:594878/141‑1 (MQ=255)

                                                                                                        tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat                                      >  2:951778/1‑141 (MQ=255)

                                                                                                         ttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagata                                     >  2:90030/1‑141 (MQ=255)

                                                                                                               gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGGCATATCCATTATCTTTGTTAGATAGAATCg                               <  1:432801/141‑1 (MQ=255)

                                                                                                                aCTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGt                              <  2:934756/141‑1 (MQ=255)

                                                                                                                 cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATTCATTATCTTTGTTAGATAGAATCGtt                             >  2:278320/1‑141 (MQ=255)

                                                                                                                 cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGtt                             <  2:272938/141‑1 (MQ=255)

                                                                                                                  acaaaaaGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCTTTTATAGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGt                              >  1:81670/4‑141 (MQ=25)

                                                                                                                     aaaaGAGATTAGTAACTTTTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTCGTTAGATAGAATCGTTGAtt                         >  1:800992/1‑141 (MQ=255)

                                                                                                                        agagATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGc                      <  2:217245/141‑1 (MQ=255)

                                                                                                                           gaTTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAat                   <  2:143668/141‑1 (MQ=255)

                                                                                                                             ttAGTATCTAATAAAATTTAATAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAatat                 >  2:490241/1‑141 (MQ=255)

                                                                                                                              tAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTTTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAATGTGACATATCCATTATCTTTGTTAGTTAGAATCGTTGAGTTGCAATAtt                >  2:158456/1‑141 (MQ=255)

                                                                                                                               aGTATCTAATAAAATTTAATAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg               >  1:289595/1‑141 (MQ=255)

                                                                                                                                gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt              >  1:192089/1‑141 (MQ=255)

                                                                                                                                    cTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTAtgt          <  2:598500/141‑1 (MQ=255)

                                                                                                                                      aTTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTgg        <  1:33427/141‑1 (MQ=255)

                                                                                                                                       ttAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGa       <  2:684360/141‑1 (MQ=255)

                                                                                                                                         aaaTTTTATTAGATACTAATCTCTTTT‑T‑TTTTTTTTTTTTATAATATGATTGATTTTATTTATAAGTACAATTGGTTTAGGGTAACATATCTATTATTTTGGTTAGATAGAATCTTTGATTTTCATTATTGAATGTGTAtt     >  2:837673/1‑141 (MQ=255)

                                                                                                                                         aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt     >  2:189212/1‑141 (MQ=255)

                                                                                                                                            ttttATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGt  >  1:661310/1‑141 (MQ=255)

GAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGT  >  CP000730/949953‑950233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`