Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	967,498	C→G	5.3%	V522V (GTC→GTG)	rexA →	ATP‑dependent nuclease Rex subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	967,498	0	C	G	5.3%	292.6 / 6.5	91	V522V (GTC→GTG)	rexA	ATP‑dependent nuclease Rex subunit A
Reads supporting (aligned to +/- strand):  ref base C (51/34);  new base G (3/2);  total (54/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

AAGAAGTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAGAAGTGCGACAT  >  CP000730/967359‑967637                                                                                                                                            |                                                                                                                                            aagaagTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTcc                                                                                                                                            <  1:311422/141‑1 (MQ=255) aagaagTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTcc                                                                                                                                            <  1:426403/141‑1 (MQ=255) aagaagTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTcc                                                                                                                                            >  2:928861/1‑141 (MQ=255)  agaagTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCt                                                                                                                                           <  1:441559/141‑1 (MQ=255)    aagTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCttg                                                                                                                                         >  1:719985/1‑141 (MQ=255)    aagTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCttg                                                                                                                                         >  1:711316/1‑141 (MQ=255)     agTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCttgt                                                                                                                                        >  2:256278/1‑141 (MQ=255)     agTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCttgt                                                                                                                                        >  1:514846/1‑141 (MQ=255)       tACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATACAGTAAACTTAAAAGTCCttgttg                                                                                                                                      >  2:20996/1‑141 (MQ=255)        aCTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGa                                                                                                                                     <  1:603320/141‑1 (MQ=255)        aCTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGa                                                                                                                                     >  2:621097/1‑141 (MQ=255)         cTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGaa                                                                                                                                    >  1:534221/1‑141 (MQ=255)            tCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCg                                                                                                                                 <  2:119167/141‑1 (MQ=255)              aaCGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGa                                                                                                                               <  1:638765/141‑1 (MQ=255)              aaCGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGa                                                                                                                               <  1:129849/141‑1 (MQ=255)                cGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATc                                                                                                                             <  1:728252/141‑1 (MQ=255)                       ctaTATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACa                                                                                                                      >  2:405242/1‑141 (MQ=255)                           atatTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGt                                                                                                                  <  1:868112/141‑1 (MQ=255)                           atatTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGt                                                                                                                  <  2:964370/141‑1 (MQ=255)                               tCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGAtt                                                                                                              <  1:977212/141‑1 (MQ=255)                                  aaCATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTaa                                                                                                           >  2:1011376/1‑141 (MQ=255)                                   aCATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAAc                                                                                                          >  1:407288/1‑141 (MQ=255)                                    cATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACt                                                                                                         >  2:515939/1‑141 (MQ=255)                                    cATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACt                                                                                                         <  2:992059/141‑1 (MQ=255)                                     atatGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTg                                                                                                        <  1:36343/141‑1 (MQ=255)                                     atatGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTg                                                                                                        <  1:36278/141‑1 (MQ=255)                                      tatGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTgg                                                                                                       >  1:931311/1‑141 (MQ=255)                                       atgatgGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGt                                                                                                      <  1:1009124/141‑1 (MQ=255)                                       atgatgGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGt                                                                                                      >  2:417116/1‑141 (MQ=255)                                       atgatgGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGCGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAACGAATCAAGAAAATCGTTATTTaaagggt                                                                                                      >  2:981960/1‑136 (MQ=255)                                        tgatgGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTa                                                                                                     <  2:584607/141‑1 (MQ=255)                                          atggatgAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGt                                                                                                   <  2:784267/141‑1 (MQ=255)                                                  aCAAGGCGGTGAAGTAAAATATGATGAAGCGGCAAAGTTGTATTATGGTGCACCATATGATGAAACGGACCATCCAGTAAACTTAAAAGACCTTGTTGAAGCGGATCAAGCACATAGAGATTTAACTGGTAGTGAACaaga                                                                                           >  2:710870/1‑141 (MQ=255)                                                     aGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgc                                                                                        >  2:495058/1‑141 (MQ=255)                                                      gTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgcg                                                                                       <  2:730494/141‑1 (MQ=255)                                                      gTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgcg                                                                                       >  2:279527/1‑141 (MQ=255)                                                      gTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgcg                                                                                       <  2:96745/141‑1 (MQ=255)                                                       tCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgcgc                                                                                      >  1:17890/1‑141 (MQ=255)                                                            gAAGTAAAATATGATGACGCGGCACAGTTGTATTCTACTGCAACCCACGATATATCGCGCCTTCCCGTAGACCTCAACCTACCTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCAtttt                                                                                 <  1:918599/141‑1 (MQ=255)                                                            gAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCAtttt                                                                                 >  2:735688/1‑141 (MQ=255)                                                              aGTAAAATATGATGACGCGGCACAGAGGTATTAAAGTGCACCCTATGATGAATCGGACGAACCAGGAAACTTAAAAGTGCTCGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTAAACAAGAAGCGCATTTtat                                                                               >  2:769429/1‑141 (MQ=255)                                                              aGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTtat                                                                               >  2:769657/1‑141 (MQ=255)                                                               gTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTtata                                                                              >  1:1070452/1‑141 (MQ=255)                                                               gTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTtata                                                                              >  1:1024199/1‑141 (MQ=255)                                                               gTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTtata                                                                              >  1:411790/1‑141 (MQ=255)                                                                tAAAATATGATGGAGCGGCACAGTGGTATTATGGTTCACCATATGATGAATCGCACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTGTAtag                                                                             <  1:917464/141‑1 (MQ=255)                                                                tAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTAtag                                                                             >  2:689543/1‑141 (MQ=255)                                                                        gatgaAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACaa                                                                     >  1:1068935/1‑141 (MQ=255)                                                                         atgaAGCGGCACAGTTGTAGTATGGTGCAACATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACACAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAAAAAg                                                                    >  2:291402/1‑141 (MQ=255)                                                                          tgaAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGt                                                                   >  1:623783/1‑141 (MQ=255)                                                                             aGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTaa                                                                >  2:767925/1‑141 (MQ=255)                                                                                ggCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGa                                                             >  1:116079/1‑141 (MQ=255)                                                                                  cacaGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGata                                                           >  1:980160/1‑141 (MQ=255)                                                                                  cacaGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGata                                                           >  1:858257/1‑141 (MQ=255)                                                                                     aGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCt                                                        <  2:992517/141‑1 (MQ=255)                                                                                     aGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCt                                                        <  2:992499/141‑1 (MQ=255)                                                                                        tGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAg                                                     >  1:1012082/1‑141 (MQ=255)                                                                                          tattatGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGaa                                                   <  2:1029085/141‑1 (MQ=255)                                                                                          tattatGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGaa                                                   >  1:307283/1‑141 (MQ=255)                                                                                           attatGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAAc                                                  >  1:524271/1‑141 (MQ=255)                                                                                            ttatGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACa                                                 >  2:379487/1‑141 (MQ=255)                                                                                            ttatGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACa                                                 >  1:731087/1‑141 (MQ=255)                                                                                               tGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCa                                              <  2:890451/141‑1 (MQ=255)                                                                                                ggTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCaa                                             <  1:1040373/141‑1 (MQ=255)                                                                                                ggTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCaa                                             <  1:268397/141‑1 (MQ=255)                                                                                                   gCACCATATGATGAATCGGACCATCCAGTAAACTTAAAGGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCaaaaa                                          >  1:574772/1‑141 (MQ=255)                                                                                                   gCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCaaaaa                                          >  1:678324/1‑141 (MQ=255)                                                                                                   gCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCaaaaa                                          >  1:207906/1‑141 (MQ=255)                                                                                                   gCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCaaaaa                                          >  2:990423/1‑141 (MQ=255)                                                                                                     aCCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGt                                        <  1:444845/141‑1 (MQ=255)                                                                                                           tgatgaATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGCACATAGGGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTtatga                                  <  1:43728/141‑1 (MQ=255)                                                                                                           tgatgaATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTtatga                                  >  2:742780/1‑141 (MQ=255)                                                                                                               gaATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAAAAAAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTtatgatatg                              >  1:520342/1‑141 (MQ=255)                                                                                                                aaTCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgcg                                                                                       <  2:901794/83‑1 (MQ=255)                                                                                                                aaTCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAgcg                                                                                       >  1:901794/1‑83 (MQ=255)                                                                                                                 aTCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGaa                            <  2:904888/141‑1 (MQ=255)                                                                                                                     gACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACa                        >  2:115335/1‑141 (MQ=255)                                                                                                                      aCCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAg                       >  2:633372/1‑141 (MQ=255)                                                                                                                       ccATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAgg                      >  2:951957/1‑141 (MQ=255)                                                                                                                       ccATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAgg                      <  1:654251/141‑1 (MQ=255)                                                                                                                          tCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAg                   <  2:86813/141‑1 (MQ=255)                                                                                                                           ccAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGaa                            <  2:837275/131‑1 (MQ=255)                                                                                                                           ccAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGaa                            >  1:837275/1‑131 (MQ=255)                                                                                                                           ccAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATATTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGc                  >  1:10970/1‑141 (MQ=255)                                                                                                                              gTAAACTTAAAAGACCTTGTTGAAGCGGAGCAAGAACATAGTGATTTAACTGGCAGTGAACAAGAAGCGCATTTTATAGCAGAACAAGTCAAAGATATCCTAGAACCTCAAACACATCATGATATGAAAAAAGGAAgacac               >  2:712356/1‑137 (MQ=255)                                                                                                                               tAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCtata              <  1:790180/141‑1 (MQ=255)                                                                                                                               tAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCtata              >  2:83442/1‑141 (MQ=255)                                                                                                                                aaaCTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCta                <  1:807437/138‑1 (MQ=255)                                                                                                                                aaaCTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCta                >  2:807437/1‑138 (MQ=255)                                                                                                                                aaaCTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAg             <  2:660003/141‑1 (MQ=255)                                                                                                                                aaaCTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCACATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAg             <  1:549754/141‑1 (MQ=255)                                                                                                                                      aaaaGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAGAAGTGc       >  1:180071/1‑141 (MQ=255)                                                                                                                                      aaaaGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAGAAGTGc       <  1:31050/141‑1 (MQ=255)                                                                                                                                           tCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAGAAGTGCGACAt  >  2:956095/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                            AAGAAGTACTGTCAACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAAACAGGAAGCTATAGAAGTGCGACAT  >  CP000730/967359‑967637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑