Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,039,416 A→C 5.8% intergenic (‑5833/‑1873) USA300HOU_0983 ← / → USA300HOU_0984 competence protein/glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,039,4160AC 5.8% 267.6 / 5.7 85intergenic (‑5833/‑1873)USA300HOU_0983/USA300HOU_0984competence protein/glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (34/46);  new base C (3/2);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AATGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAAG  >  CP000730/1039276‑1039556
                                                                                                                                            |                                                                                                                                            
aaTGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAata                                                                                                                                              <  1:279684/141‑1 (MQ=255)
aaTGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAata                                                                                                                                              >  2:351692/1‑141 (MQ=255)
 atgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAatat                                                                                                                                             <  1:773658/141‑1 (MQ=255)
 atgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAatat                                                                                                                                             <  1:612692/141‑1 (MQ=255)
  tgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGCTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGCTTTAACTATGAGATTTATCATTGCAATATTATTAGGATTAAtatt                                                                                                                                            >  1:584285/1‑141 (MQ=255)
   gatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATGTGCAGCCTTTACTATCATGGCAGACGTTTAATTAAGTTATCATGCAGTGTTGACTAGGAAATTTATCATTGCAATATTATTAGGATTAAtattat                                                                                                                                          <  2:1084355/141‑1 (MQ=255)
      gTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATcc                                                                                                                                        >  2:183343/1‑141 (MQ=255)
      gTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATcc                                                                                                                                        >  2:885849/1‑141 (MQ=255)
         aTTTGCAACGATTCTTGTCGTTCACATATTATTGCAGATTTGCAGCCTATACTATCATGGCAGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCAtt                                                                                                                                     >  2:432441/1‑141 (MQ=255)
             gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                 <  2:570505/141‑1 (MQ=255)
             gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                 <  2:707719/141‑1 (MQ=255)
                aCGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTg                                                                                                                              <  1:256569/141‑1 (MQ=255)
                   aTTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCtt                                                                                                                           <  1:639685/141‑1 (MQ=255)
                    ttCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGCTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCttt                                                                                                                          <  1:103628/141‑1 (MQ=255)
                     tCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCtttt                                                                                                                         <  2:590551/141‑1 (MQ=255)
                         gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCa                                                                                                                                       >  1:400903/1‑123 (MQ=255)
                         gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCa                                                                                                                                       <  2:400903/123‑1 (MQ=255)
                         gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACaa                                                                                                                     <  2:488342/141‑1 (MQ=255)
                            gTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGATGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCa                                                                                                                  <  1:688402/141‑1 (MQ=255)
                            gTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCa                                                                                                                  <  2:151539/141‑1 (MQ=255)
                                aaaTATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAca                                                                                                              <  1:1073468/141‑1 (MQ=255)
                                   tattatTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAcatca                                                                                                           <  1:630110/141‑1 (MQ=255)
                                       attGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCt                                                                                                       <  2:561602/141‑1 (MQ=255)
                                           cAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATAc                                                                                                   <  1:303419/141‑1 (MQ=255)
                                             gATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTCTACtt                                                                                                 >  2:420101/1‑141 (MQ=255)
                                              aTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTg                                                                                                <  1:595067/141‑1 (MQ=255)
                                               tttGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGa                                                                                               <  2:198638/141‑1 (MQ=255)
                                                 tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACCATCATACATCATCCTATACTTGATc                                                                                             <  2:565167/141‑1 (MQ=255)
                                                 tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATc                                                                                             <  2:91428/141‑1 (MQ=255)
                                                 tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATc                                                                                             <  1:18220/141‑1 (MQ=255)
                                                  gCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCg                                                                                            >  1:873927/1‑141 (MQ=255)
                                                     gCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGttt                                                                                         >  2:346644/1‑141 (MQ=255)
                                                      ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                        <  1:876357/141‑1 (MQ=255)
                                                      ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                        <  1:519879/141‑1 (MQ=255)
                                                         ataCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAAt                                                                                     >  1:465623/1‑141 (MQ=255)
                                                          taCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATc                                                                                    <  1:863350/141‑1 (MQ=255)
                                                           aCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATcc                                                                                   <  2:202306/141‑1 (MQ=255)
                                                              aTCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCttt                                                                                >  1:79394/1‑141 (MQ=255)
                                                               tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                               >  1:271725/1‑141 (MQ=255)
                                                               tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                               >  1:202464/1‑141 (MQ=255)
                                                                 aTGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCtt                                                                             <  1:502845/141‑1 (MQ=255)
                                                                 aTGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCtt                                                                             >  2:941669/1‑141 (MQ=255)
                                                                    gCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAACTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaa                                                                          <  1:854621/141‑1 (MQ=255)
                                                                      cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATTCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                        <  1:562442/141‑1 (MQ=255)
                                                                      cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                        >  2:1017362/1‑141 (MQ=255)
                                                                      cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATCAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                        >  1:785696/1‑141 (MQ=255)
                                                                       gACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAAc                                                                       <  1:470197/141‑1 (MQ=255)
                                                                       gACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAAc                                                                       <  2:395554/141‑1 (MQ=255)
                                                                           ttttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAg                                                                   <  1:719049/141‑1 (MQ=255)
                                                                            tttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGt                                                                  <  2:7675/141‑1 (MQ=255)
                                                                             ttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGta                                                                 >  2:583650/1‑141 (MQ=255)
                                                                             ttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACGTGATCGTTTTAATCCTTTCTTAAAAACGGAGta                                                                 <  1:562102/141‑1 (MQ=255)
                                                                              tAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtat                                                                >  2:147628/1‑141 (MQ=255)
                                                                               aaggAAGTTATCAGTAATTTTAAACTATTAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACAGCTTCCTATGCTTGAGCGTTTTAAACCTTTCTAAAAAACGGAGtata                                                               <  2:52248/138‑1 (MQ=255)
                                                                               aaTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                               >  2:997527/1‑141 (MQ=255)
                                                                               aaTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                               >  1:351098/1‑141 (MQ=255)
                                                                               aaTGAAGTTATCAAGGAGTTTTAAATATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAAATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                               <  2:130734/141‑1 (MQ=255)
                                                                                aTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAg                                                              >  1:75232/1‑141 (MQ=255)
                                                                                 tGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                             <  2:183943/141‑1 (MQ=255)
                                                                                 tGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                             <  1:608637/141‑1 (MQ=255)
                                                                                 tGAAGTTATCAAGGAGTTCTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTCTACATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                             <  1:214460/141‑1 (MQ=255)
                                                                                   aaGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTa                                                           <  1:727815/141‑1 (MQ=255)
                                                                                      ttATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGc                                                        <  2:860193/141‑1 (MQ=255)
                                                                                        aTCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCa                                                      >  2:885037/1‑141 (MQ=255)
                                                                                           aaGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAg                                                   <  2:912639/141‑1 (MQ=255)
                                                                                              gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                <  1:96402/141‑1 (MQ=255)
                                                                                              gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                >  2:865938/1‑141 (MQ=255)
                                                                                                     aaCTATGAAATTTATCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAgg                                         >  1:603459/1‑141 (MQ=255)
                                                                                                      aCTATGAAATTTATCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGACAAAGAGTCAAAAGGt                                        >  2:585257/1‑141 (MQ=255)
                                                                                                       cTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTa                                       <  1:226629/141‑1 (MQ=255)
                                                                                                          tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                    >  2:897149/1‑141 (MQ=255)
                                                                                                          tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                    >  2:889776/1‑141 (MQ=255)
                                                                                                            aaaTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                  >  1:649713/1‑141 (MQ=255)
                                                                                                            aaaTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                  >  1:844124/1‑141 (MQ=255)
                                                                                                              aTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaaca                                <  2:236143/141‑1 (MQ=255)
                                                                                                                 tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAata                             >  1:973939/1‑141 (MQ=255)
                                                                                                                 tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAata                             >  2:57710/1‑141 (MQ=255)
                                                                                                                 tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCCTAAAAACGGAGTCTACTTATGCCCAAGTGCCAAAAGGCACGCAACAata                             >  2:893334/1‑141 (MQ=255)
                                                                                                                  aTCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAatat                            >  1:716976/1‑141 (MQ=255)
                                                                                                                   tCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATg                           >  1:52775/1‑141 (MQ=255)
                                                                                                                   tCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATg                           >  2:265847/1‑141 (MQ=255)
                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCGAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  <  1:997527/141‑1 (MQ=255)
                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  <  2:85226/141‑1 (MQ=255)
                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  >  2:687107/1‑141 (MQ=255)
                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  <  2:452618/141‑1 (MQ=255)
                                                                                                                               tattaGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACgg               >  2:726165/1‑141 (MQ=255)
                                                                                                                                 ttaGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCt             <  2:489147/141‑1 (MQ=255)
                                                                                                                                 ttaGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCt             <  2:130656/141‑1 (MQ=255)
                                                                                                                                   aGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCtct           >  1:703180/1‑139 (MQ=255)
                                                                                                                                     gATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTtata         <  1:383730/141‑1 (MQ=255)
                                                                                                                                     gATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTtata         >  1:443713/1‑141 (MQ=255)
                                                                                                                                            attatCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAag  <  2:896466/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AATGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAAG  >  CP000730/1039276‑1039556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: