Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,250,301 C→G 6.8% T24R (ACA→AGA)  ftsY → Sec family Type I general secretory pathway protein signal recognition particle protein FtsY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,250,3010CG 6.8% 293.5 / 11.7 88T24R (ACA→AGA) ftsYSec family Type I general secretory pathway protein signal recognition particle protein FtsY
Reads supporting (aligned to +/- strand):  ref base C (47/35);  new base G (4/2);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCAGGTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCT  >  CP000730/1250163‑1250438
                                                                                                                                          |                                                                                                                                         
tCAGGTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACag                                                                                                                                         <  1:917421/141‑1 (MQ=255)
acatccattACTAAACGTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACag                                                                                                                                         <  2:585071/134‑1 (MQ=255)
   ggTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTAAAGGAGGCGCAATTATGCCCTTCTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGagaag                                                                                                                                      <  1:646914/141‑1 (MQ=255)
    gtgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGagaaga                                                                                                                                     >  1:286303/1‑141 (MQ=255)
    gtgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACagaaga                                                                                                                                     >  2:431105/1‑141 (MQ=255)
    gtgtTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACagaaga                                                                                                                                     <  1:162303/141‑1 (MQ=255)
       ttACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACa                                                                                                                                  >  1:592631/1‑141 (MQ=255)
           tAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGt                                                                                                                              <  1:576897/141‑1 (MQ=255)
            aaaCTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTc                                                                                                                             >  1:140161/1‑141 (MQ=255)
             aaCTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCa                                                                                                                            >  1:74406/1‑141 (MQ=255)
                ttGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGa                                                                                                                         <  2:790890/141‑1 (MQ=255)
                ttGTGAGTGTGAATTTAAATAAAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGa                                                                                                                         <  2:486619/141‑1 (MQ=255)
                   tgAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACaa                                                                                                                      >  2:379318/1‑141 (MQ=255)
                   tgAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaa                                                                                                                      <  1:224218/141‑1 (MQ=255)
                    gAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaaa                                                                                                                     >  2:740644/1‑141 (MQ=255)
                    gAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaaa                                                                                                                     >  2:740372/1‑141 (MQ=255)
                    gAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACaaa                                                                                                                     >  1:595924/1‑141 (MQ=255)
                     aGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAAt                                                                                                                    <  2:628504/141‑1 (MQ=255)
                        gtgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTag                                                                                                                 >  2:620678/1‑141 (MQ=255)
                         tgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTaga                                                                                                                >  2:367081/1‑141 (MQ=255)
                         tgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTaga                                                                                                                >  2:312921/1‑141 (MQ=255)
                         tgAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTaga                                                                                                                >  1:590254/1‑141 (MQ=255)
                              ttAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATa                                                                                                           <  2:1063992/141‑1 (MQ=255)
                                aaaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATaca                                                                                                         >  1:1077313/1‑141 (MQ=255)
                                 aaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTATACGTTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCACGACAAATTAGAAGATacac                                                                                                        >  2:83657/1‑141 (MQ=255)
                                 aaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATacac                                                                                                        >  2:613876/1‑141 (MQ=255)
                                 aaTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATacac                                                                                                        <  1:122861/141‑1 (MQ=255)
                                  aTACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATAcaca                                                                                                       <  1:168292/141‑1 (MQ=255)
                                    aCAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACAtt                                                                                                     >  2:331309/1‑141 (MQ=255)
                                     cAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTc                                                                                                    <  2:747232/141‑1 (MQ=255)
                                      aaTAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCt                                                                                                   >  2:418131/1‑141 (MQ=255)
                                         aGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGaa                                                                                                <  1:140512/141‑1 (MQ=255)
                                           atgatgTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAgg                                                                                              <  2:446294/141‑1 (MQ=255)
                                              atgTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTc                                                                                           <  1:578794/141‑1 (MQ=255)
                                               tgtgTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGATTAAAAGATAAGTTTGCAAGAAATAAAGAAAATGAAGAAGTTAAATACTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCa                                                                                          >  2:947898/1‑141 (MQ=255)
                                                  gtTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACg                                                                                       >  2:61578/1‑141 (MQ=255)
                                                  gtTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACg                                                                                       >  1:402224/1‑141 (MQ=255)
                                                    tGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCa                                                                                     <  1:485813/141‑1 (MQ=255)
                                                     gAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAg                                                                                    <  1:1006921/141‑1 (MQ=255)
                                                     gAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAg                                                                                    <  1:1007089/141‑1 (MQ=255)
                                                      aaGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAgg                                                                                   >  2:847475/1‑141 (MQ=255)
                                                       aggaggAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGa                                                                                  <  1:944713/141‑1 (MQ=255)
                                                          aggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGc                                                                               <  1:888442/141‑1 (MQ=255)
                                                          aggagCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGc                                                                               <  2:172001/141‑1 (MQ=255)
                                                               cAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATg                                                                          >  2:1012213/1‑141 (MQ=255)
                                                               cAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATg                                                                          <  2:636150/141‑1 (MQ=255)
                                                                   aatGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGAttt                                                                      <  1:329207/141‑1 (MQ=255)
                                                                   aatGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGAttt                                                                      <  1:1023821/141‑1 (MQ=255)
                                                                     tGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAg                                                                    <  1:778274/141‑1 (MQ=255)
                                                                         cTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGa                                                                >  1:435170/1‑141 (MQ=255)
                                                                           tttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaa                                                              <  2:246601/141‑1 (MQ=255)
                                                                            ttttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaaa                                                             <  1:151863/141‑1 (MQ=255)
                                                                             tttAAACGCTTAAATGATATTTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAAt                                                            >  2:767331/1‑141 (MQ=255)
                                                                              ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg                                                           >  2:599893/1‑141 (MQ=255)
                                                                              ttAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATg                                                           >  1:719583/1‑141 (MQ=255)
                                                                               tAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGc                                                          <  2:104817/141‑1 (MQ=255)
                                                                                aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt                                                         >  1:287988/1‑141 (MQ=255)
                                                                                aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt                                                         >  2:951133/1‑141 (MQ=255)
                                                                                aaaCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCt                                                         >  1:853086/1‑141 (MQ=255)
                                                                                  aCGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGaaaaaaaaaa                                                       <  2:658128/141‑7 (MQ=255)
                                                                                   cGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGaa                                                      >  1:637603/1‑141 (MQ=255)
                                                                                    tcTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCAGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAg                                                     >  2:249573/2‑141 (MQ=255)
                                                                                     cTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACGAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGt                                                    <  2:571867/141‑1 (MQ=255)
                                                                                     cTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGGCGCAAATGATTTAGCAGAAAATGCTGAAGt                                                    <  1:869607/141‑1 (MQ=255)
                                                                                          aaGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTAAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaa                                               >  1:10705/1‑141 (MQ=255)
                                                                                           aGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaaa                                              >  1:470079/1‑141 (MQ=255)
                                                                                           aGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGaaaaa                                              >  1:339607/1‑141 (MQ=255)
                                                                                               aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag                                          >  1:447427/1‑141 (MQ=255)
                                                                                               aaGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAaagaag                                          <  1:64085/141‑1 (MQ=255)
                                                                                                 gTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGcc                                        >  1:152161/1‑141 (MQ=255)
                                                                                                  tttGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCa                                       >  1:28576/1‑141 (MQ=255)
                                                                                                    tGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGCGAAAAAGAAGCCACg                                     >  2:670190/1‑141 (MQ=255)
                                                                                                      caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa                                   >  2:723980/1‑141 (MQ=255)
                                                                                                      caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa                                   >  2:1007214/1‑141 (MQ=255)
                                                                                                      caacaaATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCa                                   >  2:803000/1‑141 (MQ=255)
                                                                                                           aaTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTg                              >  2:986809/1‑141 (MQ=255)
                                                                                                            aTAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGa                             >  1:620032/1‑141 (MQ=255)
                                                                                                                     aTGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGACGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCgg                    >  1:1027991/1‑141 (MQ=255)
                                                                                                                     aTGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCgg                    >  1:479417/1‑141 (MQ=255)
                                                                                                                      tGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGa                   >  2:161609/1‑141 (MQ=255)
                                                                                                                         agaagTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACAATCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGAttt                <  2:731156/141‑1 (MQ=255)
                                                                                                                                aaaTCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGAc         >  1:377706/1‑141 (MQ=255)
                                                                                                                                 aaTCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACg        >  1:1042885/1‑141 (MQ=255)
                                                                                                                                    ccTTAAGCGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg     >  1:859345/1‑141 (MQ=255)
                                                                                                                                    ccTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTGCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATg     <  2:190379/141‑1 (MQ=255)
                                                                                                                                       tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt  <  1:117502/141‑1 (MQ=255)
                                                                                                                                       tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt  <  1:133090/141‑1 (MQ=255)
                                                                                                                                       tAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCt  >  1:27715/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TCAGGTGTTACTAAACTTGTGAGTGTGAATTTAAATACAATAGATGATGTGTTGAAGGAGGAGCAATAATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTTAAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCAACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTGAGTGAAGCGGATTTTGATGACGATGGCT  >  CP000730/1250163‑1250438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: