Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,319,376 A→G 7.5% I504V (ATA→GTA)  miaB → 2‑methylthioadenine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,319,3760AG 7.5% 223.4 / 7.9 80I504V (ATA→GTA) miaB2‑methylthioadenine synthetase
Reads supporting (aligned to +/- strand):  ref base A (40/34);  new base G (2/4);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGCACAGATT  >  CP000730/1319236‑1319513
                                                                                                                                            |                                                                                                                                         
gtaAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTta                                                                                                                                           <  2:80523/141‑1 (MQ=255)
gtaAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTta                                                                                                                                           >  2:804174/1‑141 (MQ=255)
 taAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTtat                                                                                                                                          >  2:353031/1‑141 (MQ=255)
 taAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTtat                                                                                                                                          <  1:682623/141‑1 (MQ=255)
   aaaaaGATGATCAGGTTCTTACTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATaa                                                                                                                                        >  1:193474/1‑141 (MQ=255)
    aaaaGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATaaa                                                                                                                                       <  1:244927/141‑1 (MQ=255)
      aaGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAgg                                                                                                                                     <  1:980298/141‑1 (MQ=255)
       aGATTATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGa                                                                                                                                    <  1:761694/141‑1 (MQ=255)
       aGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGa                                                                                                                                    <  2:1008511/141‑1 (MQ=255)
       aGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGa                                                                                                                                    <  2:600727/141‑1 (MQ=255)
         atgatCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAg                                                                                                                                  <  1:360342/141‑1 (MQ=255)
            atgaGGTTCTTGCTGGATACACTGATAAAAATACGCTAGTTAATTTCAAAGCGCCTAAAGAAATGCTTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTACGTGGCAGTTTTATAAAGGAAGTag                                                                                                                               >  2:539793/4‑141 (MQ=255)
             tCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTATTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTaga                                                                                                                              >  2:951486/1‑141 (MQ=255)
             tCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTaga                                                                                                                              >  2:762771/1‑141 (MQ=255)
              cAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTagag                                                                                                                             <  1:79350/141‑1 (MQ=255)
                 gTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCg                                                                                                                          >  1:412653/1‑141 (MQ=255)
                 gTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCg                                                                                                                          <  1:714684/141‑1 (MQ=255)
                 gTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCg                                                                                                                          <  2:510407/141‑1 (MQ=255)
                  tacTTGCTGGCTACCCTGATAAAAATAAGCTAGTTAATTGCAACGCGCCTAAAGGAATGATTGGTAAACCAGTGGAAGTACGAAGAGATGAAGCTAATCAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCgg                                                                                                                         <  1:586059/139‑1 (MQ=255)
                    cTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTAGAGCCGGaa                                                                                                                       <  1:370962/141‑1 (MQ=255)
                        cTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATgg                                                                                                                   >  1:49060/1‑141 (MQ=255)
                          ggatACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTg                                                                                                                 <  2:956104/138‑1 (MQ=255)
                          ggCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTg                                                                                                                 >  2:496520/1‑141 (MQ=255)
                          ggCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTg                                                                                                                 >  2:154607/1‑141 (MQ=255)
                           gCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTAGAGCCGGAAATGGTGa                                                                                                                <  1:350889/141‑1 (MQ=255)
                            cTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGAt                                                                                                               <  2:400854/141‑1 (MQ=255)
                              acacTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTc                                                                                                             >  2:407773/1‑141 (MQ=255)
                                           tAAGCTTGTTAATTGCAAAGCGCCTAACGAAATGATTGGTAAACTAGTGGAAGTACAAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataa                                                                                                <  1:309551/141‑1 (MQ=255)
                                           tAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataa                                                                                                >  2:544610/1‑141 (MQ=255)
                                             aGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataata                                                                                              >  1:767455/1‑141 (MQ=255)
                                             aGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataata                                                                                              >  1:181428/1‑141 (MQ=255)
                                                 aGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGa                                                                                          >  1:620435/1‑141 (MQ=255)
                                                 aGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGa                                                                                          >  1:14245/1‑141 (MQ=255)
                                                    tAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGa                                                                                       <  1:936790/141‑1 (MQ=255)
                                                     aaTTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGCACCTAAACAGTATGCATTAAATGGCAGTCTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGAc                                                                                      <  1:757945/141‑1 (MQ=255)
                                                         tccaaGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATACAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACgtgt                                                                                  <  2:505966/138‑1 (MQ=255)
                                                         tCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACgtgt                                                                                  <  2:498977/141‑1 (MQ=255)
                                                          cAAAGCGACTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGTAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGtt                                                                                 <  2:998486/141‑1 (MQ=255)
                                                           aaaGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTg                                                                                >  2:109726/1‑141 (MQ=255)
                                                            aaGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGa                                                                               >  2:1039715/1‑141 (MQ=255)
                                                            aaGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGa                                                                               >  2:421261/1‑141 (MQ=255)
                                                              gcaccTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGaaa                                                                             <  2:315119/138‑2 (MQ=255)
                                                               cgcCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAAcc                                                                            >  2:461850/1‑141 (MQ=255)
                                                                gcCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCa                                                                           >  2:363625/1‑141 (MQ=255)
                                                                  cTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAg                                                                         <  1:464863/141‑1 (MQ=255)
                                                                  cTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAg                                                                         >  1:60790/1‑141 (MQ=255)
                                                                   tAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGc                                                                        <  1:965944/141‑1 (MQ=255)
                                                                    aaaGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCg                                                                       >  1:822711/1‑141 (MQ=255)
                                                                      aGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGa                                                                     <  2:520086/141‑1 (MQ=255)
                                                                       gAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGat                                                                    <  1:1013493/141‑1 (MQ=255)
                                                                           tGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGataata                                                                >  2:491875/1‑141 (MQ=255)
                                                                            gATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAatat                                                               >  1:735240/1‑141 (MQ=255)
                                                                             aTTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAAACAAGCGGATAATAtt                                                              >  1:418326/1‑141 (MQ=255)
                                                                                  tAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCaaa                                                         <  2:761034/141‑1 (MQ=255)
                                                                                        aGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAAt                                                   <  1:501735/141‑1 (MQ=255)
                                                                                          tGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTa                                                 >  2:349476/1‑141 (MQ=255)
                                                                                           ggAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTaa                                                >  2:371557/1‑141 (MQ=255)
                                                                                              aGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTaaaaa                                             >  2:1022211/1‑141 (MQ=255)
                                                                                               gTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAAt                                            <  1:1071865/141‑1 (MQ=255)
                                                                                                 aCGAATAGATGAAGCTAAACAGTATTAATAAAAAGAAAGTATTATAAAGGAAGTAGAGCCGGAAAGGGGGATTCAATAAATGTATAATAAAGATGACGTGTTGAGCCAAGCGTATAATATTGAAAAAAAAAAAAAAAAttt                                          <  2:842821/141‑1 (MQ=255)
                                                                                                  cGAATAGATTAAGCTAAAAAGTATTCATTAAATGGCAGGTTTAGAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTAAACCAAGCGGATAATAATGCAAATAAAATTAAAAATTTg                                         >  2:298358/1‑141 (MQ=255)
                                                                                                    aaTAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAATTAAAAATTTGGAt                                      >  2:478574/1‑141 (MQ=255)
                                                                                                     aTAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAAAAAAAATAAAAATTTGGAt                                      <  2:84533/141‑1 (MQ=255)
                                                                                                      tAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATa                                     >  2:516028/1‑141 (MQ=255)
                                                                                                        gATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACt                                   >  2:530657/1‑141 (MQ=255)
                                                                                                         aTGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTa                                  <  2:757790/141‑1 (MQ=255)
                                                                                                            aaGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCa                               >  1:198634/1‑141 (MQ=255)
                                                                                                                    cAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAAACAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACatat                       >  2:755838/1‑141 (MQ=255)
                                                                                                                    cAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACatat                       >  2:391919/1‑141 (MQ=255)
                                                                                                                    cAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACataa                       >  1:513138/1‑140 (MQ=255)
                                                                                                                       tATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATcaa                    <  1:849460/141‑1 (MQ=255)
                                                                                                                       gaTTCATTAGATGGCAGTTTTATGAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAGCCAAGCGGATAATATTGCAAATAAAATTAAAAAGTTGGATACTATCAAAACATATcaa                    <  2:756427/140‑1 (MQ=255)
                                                                                                                        aTTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATcaac                   <  2:904103/141‑1 (MQ=255)
                                                                                                                         ttCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATcaaca                  >  2:22550/1‑141 (MQ=255)
                                                                                                                          tCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATcaacaa                 >  1:420083/1‑141 (MQ=255)
                                                                                                                            aTTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAAt               <  1:851220/141‑1 (MQ=255)
                                                                                                                             ttAAATGGCAGGTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTAGAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAAtt              >  2:377869/1‑141 (MQ=255)
                                                                                                                                 aTGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAg          <  1:459968/141‑1 (MQ=255)
                                                                                                                                  tGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGc         >  2:163650/1‑141 (MQ=255)
                                                                                                                                  tGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGc         <  2:205590/141‑1 (MQ=255)
                                                                                                                                     cAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGcaca      >  1:303952/1‑141 (MQ=255)
                                                                                                                                      aGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGCACAg     >  2:327915/1‑141 (MQ=255)
                                                                                                                                      aGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGCACAg     >  2:717502/1‑141 (MQ=255)
                                                                                                                                         tttATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGCACAGAtt  <  2:487096/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
GTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATATCAACAAATTGAAGCACAGATT  >  CP000730/1319236‑1319513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: