Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,341,749 G→T 5.6% intergenic (+157/‑355) USA300HOU_1245 → / → USA300HOU_1246 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,341,7490GT 5.6% 265.2 / 3.7 81intergenic (+157/‑355)USA300HOU_1245/USA300HOU_1246hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (44/31);  new base T (3/2);  total (47/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAATCAAGCTATGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGT  >  CP000730/1341609‑1341888
                                                                                                                                            |                                                                                                                                           
aTTAATCAAGCTATGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACg                                                                                                                                             >  2:94463/1‑141 (MQ=255)
    aTCAAGCTATGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATTGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAAt                                                                                                                                         >  2:919603/1‑141 (MQ=255)
    aTCAAGCTATGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAAt                                                                                                                                         >  1:468720/1‑141 (MQ=255)
     tCAAGCTNTGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTACAATTTATTGCACGAAAtt                                                                                                                                        <  2:816902/141‑1 (MQ=255)
     tCAAGCTATGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAAtt                                                                                                                                        >  2:20806/1‑141 (MQ=255)
                  aTTTGATTTAACTATTAATAAAAATCATATGGTAAATGGATATTATAATGATTAAAATACAAAAATAGTACATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCGTTTAGAATGTATTACACTAAATTAGCATCGAATAAg                                                                                                                           >  1:483140/1‑141 (MQ=255)
                      gATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGaa                                                                                                                       >  2:646146/1‑141 (MQ=255)
                      gATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGaa                                                                                                                       >  1:147156/1‑141 (MQ=255)
                      gATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGaa                                                                                                                       >  1:1065953/1‑141 (MQ=255)
                        tttAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGaaaa                                                                                                                     >  1:42629/1‑141 (MQ=255)
                         ttAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAAt                                                                                                                    <  1:806064/141‑1 (MQ=255)
                         ttAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAAGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAAt                                                                                                                    <  1:806062/141‑1 (MQ=255)
                            aCTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATaat                                                                                                                 >  2:207381/1‑140 (MQ=255)
                               aTTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAgtgt                                                                                                              >  1:424719/1‑141 (MQ=255)
                                 taataaAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTa                                                                                                            <  2:45171/141‑1 (MQ=255)
                                 taataaAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTa                                                                                                            <  2:147070/141‑1 (MQ=255)
                                 taacaaAAATAATATGATGAATGTATATTATAATAAAAAAAAAAAAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTa                                                                                                            <  2:50182/141‑1 (MQ=255)
                                      aaaaTCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGttt                                                                                                       <  1:927111/141‑1 (MQ=255)
                                       aaaTCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGtttt                                                                                                      >  2:328185/1‑141 (MQ=255)
                                       aaaTCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGtttt                                                                                                      >  1:491687/1‑141 (MQ=255)
                                       aaaTCATATGGTAAATGGATATTATAATGATTAAAATACAAAAATAGTACATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTACACTAAATTAGCATCGAATAAGGGAAAATGAGTGTTAAGtttt                                                                                                      >  2:190675/1‑141 (MQ=255)
                                        aaTCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTa                                                                                                     >  1:236199/1‑141 (MQ=255)
                                         aTCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTaa                                                                                                    <  2:908175/141‑1 (MQ=255)
                                            atatGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATg                                                                                                 >  2:82241/1‑141 (MQ=255)
                                            atatGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATg                                                                                                 >  1:586868/1‑141 (MQ=255)
                                            atatGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATg                                                                                                 >  1:134415/1‑141 (MQ=255)
                                                    aaTGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATaaaaaa                                                                                         <  2:502957/141‑1 (MQ=255)
                                                     aTGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAg                                                                                        <  2:937316/141‑1 (MQ=255)
                                                      tGGATATTATAATGATTAAGATTTAAAAATAGTACATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTACACTTTATTAGCATCGAATAAGGGAAAATGAGTGTTAAGTTTTAAATGATAAAATAGa                                                                                       <  2:357959/141‑1 (MQ=255)
                                                         atatTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTg                                                                                    <  2:481946/141‑1 (MQ=255)
                                                          tattatAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTgg                                                                                   >  1:45979/1‑141 (MQ=255)
                                                              ataataATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATg                                                                               <  2:346053/141‑1 (MQ=255)
                                                                aataatTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGa                                                                             <  1:948725/141‑1 (MQ=255)
                                                                  taatTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATc                                                                           >  1:596637/1‑141 (MQ=255)
                                                                    atTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGCCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTGTTAAATGAGAAAAAAGATTGGAATGGATCGt                                                                         >  2:454950/1‑141 (MQ=255)
                                                                      tAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCt                                                                       >  1:335370/1‑141 (MQ=255)
                                                                        aaaTACAAAAATAGTAGATTCCAATTTGTGAGGATGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTg                                                                     <  1:782133/141‑1 (MQ=255)
                                                                         aaTACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGa                                                                    <  1:1000885/141‑1 (MQ=255)
                                                                         aaTACAAAAATAGTACATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTACACTAAATTAGCATCGAATAAGGGAAAATGAGTGTTAAGTTTTAACTGCTAAACTAGATTGGAATGGATCGTCTTGa                                                                    >  1:762120/1‑141 (MQ=255)
                                                                              aaaaaTAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATTGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGc                                                               <  1:399802/141‑1 (MQ=255)
                                                                               aaaaTAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCt                                                              <  1:736399/141‑1 (MQ=255)
                                                                                aaaTAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTc                                                             >  2:886013/1‑141 (MQ=255)
                                                                                  aTAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTccc                                                           >  1:591297/1‑141 (MQ=255)
                                                                                    agtagATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCtt                                                         >  2:738103/1‑141 (MQ=255)
                                                                                    agtagATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCtt                                                         >  2:694239/1‑141 (MQ=255)
                                                                                    agtagATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCtt                                                         >  2:384034/1‑141 (MQ=255)
                                                                                      taaaTTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCa                                                       <  2:762920/138‑1 (MQ=255)
                                                                                       agATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCaa                                                      >  1:601768/1‑141 (MQ=255)
                                                                                            ccAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGttt                                                 <  2:52379/141‑1 (MQ=255)
                                                                                             cAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGtttt                                                >  2:237116/1‑141 (MQ=255)
                                                                                               aTTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCa                                              >  2:238312/1‑141 (MQ=255)
                                                                                                 ttGTGAAATAGGAGTATATTGCTGTATATAGAATTTATTGCACGAAATTAGCATCGAATAAGGGACAATAAGTGTTAAGTTATAAATGCAAAAAAAGATTGGAATGGATCGTCTAGAAAGGCTCCCtacgcagtgtgcaca                                            <  2:688575/141‑15 (MQ=255)
                                                                                                  tgtgAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGAACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCAttt                                           >  1:1063354/1‑141 (MQ=255)
                                                                                                   gtgAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCAtttt                                          >  1:353862/1‑141 (MQ=255)
                                                                                                   gtgAAAAAAGAGTATGTCGTTTCATTTAAAATTTATTGCACAAAATTAGCATAAAAAAAAAAAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCAtttt                                          <  2:231793/141‑1 (MQ=255)
                                                                                                     gAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCAtttttt                                        <  1:194976/141‑1 (MQ=255)
                                                                                                        aaaGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCaa                                     <  1:827868/141‑1 (MQ=255)
                                                                                                          aGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATg                                   <  2:143200/141‑1 (MQ=255)
                                                                                                              gTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGa                               >  2:667070/1‑141 (MQ=255)
                                                                                                               tATGTTGTTTCGTTTAGAATTTTTTTCTCGAGACTATCGTCTTATACGGGTAAATTAGTGTTATGTTTTCATTGATTATTAACCTTTGTATGGTTCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTCTGAATgacgac                              >  2:114822/1‑141 (MQ=255)
                                                                                                               tATGTCGTTTCGTTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGACCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGAc                              >  2:618988/1‑141 (MQ=255)
                                                                                                               tATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGAc                              >  2:244204/1‑141 (MQ=255)
                                                                                                                aTGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACt                             <  2:132368/141‑1 (MQ=255)
                                                                                                                 tGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACtt                            >  2:599711/1‑141 (MQ=255)
                                                                                                                 tGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACtt                            >  2:843585/1‑141 (MQ=255)
                                                                                                                 tGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACtt                            >  2:308495/1‑141 (MQ=255)
                                                                                                                   tCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCtt                                                                      <  2:254192/97‑1 (MQ=255)
                                                                                                                   tCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCtt                                                                      >  1:254192/1‑97 (MQ=255)
                                                                                                                     gTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGaa                        >  1:799529/1‑141 (MQ=255)
                                                                                                                          aTTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAggggg                   >  2:1070687/1‑141 (MQ=255)
                                                                                                                          aTTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAggggg                   >  2:1070724/1‑141 (MQ=255)
                                                                                                                           tttAGAATTTATTACACTAAATTAGCATCGAATAAGGGAAAATGAGTGTTAAGTTTTAAATGATAAAATAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCTACTTCGAAggggca                  <  2:385995/141‑3 (MQ=38)
                                                                                                                            ttAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCa                 >  2:941684/1‑141 (MQ=255)
                                                                                                                             tAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCAt                >  2:684333/1‑141 (MQ=255)
                                                                                                                              aGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCAtt               <  2:30632/141‑1 (MQ=255)
                                                                                                                               gAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCAttt              <  1:616532/141‑1 (MQ=255)
                                                                                                                               gAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCAttt              <  1:837716/141‑1 (MQ=255)
                                                                                                                                 aTTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTc            >  2:315297/1‑141 (MQ=255)
                                                                                                                                  tttATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCa           >  2:885126/1‑141 (MQ=255)
                                                                                                                                   ttATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCAt          <  1:394025/141‑1 (MQ=255)
                                                                                                                                   ttATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAACGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCAt          <  2:450591/141‑1 (MQ=255)
                                                                                                                                           cGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGt  <  1:94463/141‑1 (MQ=255)
                                                                                                                                           agAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGt  <  2:716367/140‑1 (MQ=255)
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ATTAATCAAGCTATGTTTATTTGATTTAACTATTAATAAAAATCATATGGTGAATGGATATTATAATAATTAAAATACAAAAATAGTAGATTCCAATTTGTGAAAAAGGAGTATGTCGTTTCATTTAGAATTTATTGCACGAAATTAGCATCGAATAAGGGAAAATAAGTGTTAAGTTTTAAATGATAAAAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGT  >  CP000730/1341609‑1341888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: