Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,436,255 T→A 6.5% T250T (ACT→ACA)  hipO → M20D subfamily hippurate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,436,2550TA 6.5% 277.6 / 7.4 92T250T (ACT→ACA) hipOM20D subfamily hippurate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base A (3/3);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

ACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAA  >  CP000730/1436117‑1436395
                                                                                                                                          |                                                                                                                                            
acaacaTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTAt                                                                                                                                            >  1:155820/1‑141 (MQ=255)
 caacaTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTAtt                                                                                                                                           <  2:793556/141‑1 (MQ=255)
  aacaTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTc                                                                                                                                          >  2:968527/1‑141 (MQ=255)
     aTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTa                                                                                                                                       >  1:300612/1‑141 (MQ=255)
     aTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTCTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTa                                                                                                                                       <  1:205058/141‑1 (MQ=255)
       gTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACa                                                                                                                                     >  1:285447/1‑141 (MQ=255)
        tATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACat                                                                                                                                    <  2:971303/141‑1 (MQ=255)
         aTTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACata                                                                                                                                   <  1:1040134/141‑1 (MQ=255)
          ttAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACatat                                                                                                                                  >  1:496337/1‑141 (MQ=255)
           tAACTCAAATATCACAAATTCGACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATg                                                                                                                                 >  2:944628/1‑141 (MQ=255)
           tAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACATATg                                                                                                                                 <  2:894346/141‑1 (MQ=255)
           tAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATg                                                                                                                                 <  2:76249/141‑1 (MQ=255)
             aCTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGat                                                                                                                               >  2:625836/1‑141 (MQ=255)
              cTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGata                                                                                                                              >  2:851958/1‑141 (MQ=255)
              cTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGCACAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGata                                                                                                                              >  1:19776/1‑141 (MQ=255)
              cTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCCGCTCAGGAGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGata                                                                                                                              >  1:19661/1‑141 (MQ=255)
                  aaTATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATtga                                                                                                                          <  1:191962/141‑1 (MQ=255)
                     atCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATtgatga                                                                                                                       <  2:990768/141‑1 (MQ=255)
                      tCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTgatgat                                                                                                                      <  2:754760/141‑1 (MQ=255)
                        acaAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGAGATTAAAAATCTGTTAAGTAATCGCTATTTCGAACGTACTATTCGTCAATATGATATTGATGAttt                                                                                                                    >  1:643961/1‑141 (MQ=255)
                          aaaTTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCACGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTaa                                                                                                                  >  2:338148/1‑141 (MQ=255)
                            aTTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTTATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACa                                                                                                                >  2:902999/1‑141 (MQ=255)
                            aTTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACa                                                                                                                >  2:921319/1‑141 (MQ=255)
                             ttCAACAATTTCACCTTATCGGTGTTAAACGAAATATTGTTCATATTGGTCATTTTAAAGGTGGTGAAGCGATTATCACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACaa                                                                                                               <  1:958179/141‑1 (MQ=255)
                             ttCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTTATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAGGGTACTATTCGTACATATGATATTGATGATTTAACaa                                                                                                               <  1:10729/141‑1 (MQ=255)
                                 acaaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACAATCGt                                                                                                           >  2:274506/1‑141 (MQ=255)
                                 acaaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGt                                                                                                           >  2:805951/1‑141 (MQ=255)
                                   aaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTa                                                                                                         <  1:659385/141‑1 (MQ=255)
                                      ttCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTaaaa                                                                                                      >  2:286008/1‑141 (MQ=255)
                                       tCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTaaggt                                                                                                     >  1:835042/1‑138 (MQ=255)
                                         aCCTTAACGGTTTGAATTGCTATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATc                                                                                                   <  1:283491/141‑1 (MQ=255)
                                         aCCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATc                                                                                                   >  2:260595/1‑141 (MQ=255)
                                         aCCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATc                                                                                                   >  2:605263/1‑141 (MQ=255)
                                            ttAGCGGTTTGAAACGAAATATAGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTCTCTAGCAGATACTACTCGTACAAATGACATTGATCATTTAACAATCGTTAAACATCaaa                                                                                                <  2:918431/141‑1 (MQ=255)
                                               aCGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTGGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGc                                                                                             <  2:153198/141‑1 (MQ=255)
                                                cGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGca                                                                                            <  1:802186/141‑1 (MQ=255)
                                                 ggTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGAT‑TTTTAACGATTGTTaaatcatcaatatcat                                                                                          <  2:453481/141‑14 (MQ=38)
                                                    ttGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAg                                                                                        >  1:211814/1‑141 (MQ=255)
                                                     tGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGa                                                                                       >  1:229359/1‑141 (MQ=255)
                                                     tGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGa                                                                                       <  2:964341/141‑1 (MQ=255)
                                                     tGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGAGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAAGCGTTAAAAATCAAATGCACAAGa                                                                                       <  2:89245/141‑1 (MQ=255)
                                                       aaaCGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATa                                                                                     <  1:775335/141‑1 (MQ=255)
                                                       aaaCGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATa                                                                                     <  1:610617/141‑1 (MQ=255)
                                                           gAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAg                                                                                 >  1:291901/1‑141 (MQ=255)
                                                            aaaTATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGa                                                                                <  1:835973/141‑1 (MQ=255)
                                                               tatTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAg                                                                             >  2:478465/1‑141 (MQ=255)
                                                                atTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgt                                                                            <  1:668398/141‑1 (MQ=255)
                                                                atTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgt                                                                            >  2:525647/1‑141 (MQ=255)
                                                                 ttGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATTATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCATAAAgtg                                                                           >  2:430060/1‑141 (MQ=255)
                                                                 ttGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgtg                                                                           >  2:430042/1‑141 (MQ=255)
                                                                  tGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgtgt                                                                          <  1:1025779/141‑1 (MQ=255)
                                                                   gTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTc                                                                         >  1:680291/1‑141 (MQ=255)
                                                                     tCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCaa                                                                       >  1:834242/1‑141 (MQ=255)
                                                                           tGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCt                                                                 <  1:308371/141‑1 (MQ=255)
                                                                            ggTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTg                                                                >  2:1078274/1‑141 (MQ=255)
                                                                             gTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGt                                                               >  2:943557/1‑141 (MQ=255)
                                                                               cATTTTAAAGCTGGTGAAGTGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGttt                                                             >  2:326184/1‑141 (MQ=255)
                                                                               cATTTTAAAGCTGGTGAAGTGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGttt                                                             >  2:326224/1‑141 (MQ=255)
                                                                               cATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGttt                                                             <  2:786392/141‑1 (MQ=255)
                                                                                aTTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTa                                                            >  2:983752/1‑141 (MQ=255)
                                                                                     agaGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTa                                                       <  2:706234/139‑1 (MQ=255)
                                                                                     aaaGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTa                                                       <  1:1041322/141‑1 (MQ=255)
                                                                                       aGCTGGTGAAGCGATTAACACTGTACGAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACACTCGTTAAAACTCAAATGCACCAGATAGCAGCAAGTGTCAAGCTTCCGTTTAATGTAGa                                                     >  1:48165/1‑141 (MQ=255)
                                                                                         cTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAtt                                                   >  1:1036529/1‑141 (MQ=255)
                                                                                           ggTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATtgt                                                 >  1:11613/1‑141 (MQ=255)
                                                                                            gTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATtgtg                                                <  2:234703/141‑1 (MQ=255)
                                                                                             tGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGa                                               <  2:760327/141‑1 (MQ=255)
                                                                                               aaGCGAGTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGGACATATGATATTGATGATTTAACAATAGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGGTTGTGGAg                                             >  1:835701/1‑141 (MQ=255)
                                                                                                aGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGt                                            <  2:823899/141‑1 (MQ=255)
                                                                                                 gCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGtt                                           <  1:779010/141‑1 (MQ=255)
                                                                                                    aTTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTaaa                                        <  1:388166/141‑1 (MQ=255)
                                                                                                     ttAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAt                                       <  2:997806/141‑1 (MQ=255)
                                                                                                     ttAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAt                                       <  1:776066/141‑1 (MQ=255)
                                                                                                       aaCACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAttt                                     >  1:408057/1‑141 (MQ=255)
                                                                                                        acacTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTg                                    >  1:852333/1‑141 (MQ=255)
                                                                                                        acacTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTg                                    <  2:442112/141‑1 (MQ=255)
                                                                                                              tACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAg                              <  1:128220/141‑1 (MQ=255)
                                                                                                                   aGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTAt                         >  1:27931/1‑141 (MQ=255)
                                                                                                                    gttattgCTATGGAGAAGGTACTATTCGTACATATTATATTGATGATTTAACTATCGGTAAAAATCAAATGCACAAGATAGCAGAAAGTGGCAAGCTTCTGTTTAATGTGTATTGTGTTGTTAAATTTGCAGAAGGTTATc                        <  2:281279/135‑1 (MQ=255)
                                                                                                                    gTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATc                        >  1:653993/1‑141 (MQ=255)
                                                                                                                        tGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATccccc                    <  1:861881/141‑1 (MQ=255)
                                                                                                                         ggCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCt                   >  2:550571/1‑141 (MQ=255)
                                                                                                                          gttATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAATGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  <  2:1050070/139‑1 (MQ=255)
                                                                                                                          gttATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAATGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  <  2:1049995/139‑1 (MQ=255)
                                                                                                                          gCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  <  1:377787/141‑1 (MQ=255)
                                                                                                                          gCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  >  2:293582/1‑141 (MQ=255)
                                                                                                                           cTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAc                 >  1:562012/1‑141 (MQ=255)
                                                                                                                                tAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatca            >  1:848934/1‑141 (MQ=255)
                                                                                                                                 aGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatcaa           >  1:305693/1‑141 (MQ=255)
                                                                                                                                  gAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatcaat          >  2:676146/1‑141 (MQ=255)
                                                                                                                                    aGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAGGCTTCTGTTTAATTGAGATTGTGTAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAg        >  1:758322/1‑141 (MQ=255)
                                                                                                                                       tACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTcc     <  1:6384/141‑1 (MQ=255)
                                                                                                                                          tATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGaa  >  1:278777/1‑141 (MQ=255)
                                                                                                                                          tATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGaa  >  2:1062279/1‑141 (MQ=255)
                                                                                                                                          tATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGaa  >  2:312639/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAA  >  CP000730/1436117‑1436395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑