Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,814,162 G→A 6.2% V360V (GTC→GTT dnaE2 ← DNA‑directed DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,814,1620GA 6.2% 239.3 / 2.3 79V360V (GTC→GTTdnaE2DNA‑directed DNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (32/42);  new base A (3/2);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CTTGAAATTTCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCAATAAATAACTGACCAG  >  CP000730/1814022‑1814299
                                                                                                                                            |                                                                                                                                          
cTTGAAATTTCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATg                                                                                                                                            >  1:249197/1‑141 (MQ=255)
cTTGAAATTTCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATg                                                                                                                                            >  1:2861/1‑141 (MQ=255)
cTTGAAATTTCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATg                                                                                                                                            >  1:426315/1‑141 (MQ=255)
 atgaagttacATTAAATGAAATTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGa                                                                                                                                           >  1:569706/10‑141 (MQ=255)
   gAAATTTCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGAcc                                                                                                                                        >  1:688430/1‑141 (MQ=255)
       tttCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCttt                                                                                                                                    >  2:727792/1‑141 (MQ=255)
                 tGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCgtgt                                                                                                                          <  1:988160/141‑1 (MQ=255)
                    aaCTTCATCAAACCCCATAATTCTGCCAACTTCTCGATCAACTGCTCTTTCAAGCATATGAGCGAAATTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTGGGACGTACTGAATGACCCTTTCTCGGCGTGTATc                                                                                                                       <  1:593596/141‑1 (MQ=255)
                     aCTTCATCAAACCCCATAATTCGTCCAACATCCCTAGCAACCGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATAACCCTTTCTCGGCGTGTATCt                                                                                                                      >  2:1058827/1‑141 (MQ=255)
                      cTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCACCTGCTCTTGCAAGCAGATGACCGATAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCtt                                                                                                                     <  1:376044/141‑1 (MQ=255)
                            caAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGt                                                                                                               <  1:396196/141‑1 (MQ=255)
                            caAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGt                                                                                                               >  1:397952/1‑141 (MQ=255)
                            caAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTATCTTGGACATAATCAATGACTCTTTCTCAGCGAGTATCTTCAAAGt                                                                                                               >  1:856428/1‑141 (MQ=255)
                             aaaCCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGtc                                                                                                              >  2:988804/1‑141 (MQ=255)
                             aaaCCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGtc                                                                                                              >  2:95306/1‑141 (MQ=255)
                             aaaCCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGtc                                                                                                              >  2:95279/1‑141 (MQ=255)
                             aaaCCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGtc                                                                                                              <  1:803211/141‑1 (MQ=255)
                                ccccATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGtcaat                                                                                                           <  1:682154/141‑1 (MQ=255)
                                ccccATAATTCGTCCAACATCCCTAGCAACCGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATAACCCTTTCTCGGCGTGTATCTTCAAAGtcaat                                                                                                           >  1:448320/1‑141 (MQ=255)
                                           ttcCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAg                                                                                                >  1:757682/1‑141 (MQ=255)
                                           ttcCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAg                                                                                                <  1:95306/141‑1 (MQ=255)
                                           ttcCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAg                                                                                                <  1:95279/141‑1 (MQ=255)
                                            tcCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAgg                                                                                               >  1:904911/1‑141 (MQ=255)
                                                aCATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCAtt                                                                                           >  2:218851/1‑141 (MQ=255)
                                                 cATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTg                                                                                          >  1:1041187/1‑141 (MQ=255)
                                                     tctGGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTac                                                                                      <  2:88802/141‑1 (MQ=255)
                                                     tctAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTac                                                                                      <  1:363185/141‑1 (MQ=255)
                                                     tctAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTac                                                                                      <  2:512583/141‑1 (MQ=255)
                                                     tctAGCAACTGCTATTGCAAGCAGATGACCGAAAGTCATAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTac                                                                                      <  1:17836/141‑1 (MQ=255)
                                                       tAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTacac                                                                                    <  1:981616/141‑1 (MQ=255)
                                                          catctgcttgcaagaGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGtt                                                                                 >  2:347456/15‑141 (MQ=255)
                                                            aCTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCt                                                                               >  2:519054/1‑141 (MQ=255)
                                                                ttctTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTTGCTCGCCTTATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTTTATCTTCAAAGTCAATATCAATATCAGGCATTGTTATTCGTTCTGGGt                                                                           <  2:1005600/140‑1 (MQ=255)
                                                                ctctTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGt                                                                           <  1:788552/141‑1 (MQ=255)
                                                                ctctTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGt                                                                           <  1:653014/141‑1 (MQ=255)
                                                                ctctTGAAAGCAGATAACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATAACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCTATATCAGGCATTGTTACACGTTCTGGGt                                                                           <  2:511909/141‑1 (MQ=255)
                                                                  ctTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGttt                                                                         >  2:791490/1‑141 (MQ=255)
                                                                   ttGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTa                                                                        <  2:789973/141‑1 (MQ=255)
                                                                    tGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTaa                                                                       <  2:426045/141‑1 (MQ=255)
                                                                     gCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTaaa                                                                      <  1:544031/141‑1 (MQ=255)
                                                                     gCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATAACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCTATATCAGGCATTGTTACACGTTCTGGGTTTaaa                                                                      >  1:966765/1‑141 (MQ=255)
                                                                        aGCAGTTGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTaaaaaa                                                                   >  1:606097/1‑141 (MQ=255)
                                                                        aGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTGTCGCGGCGTGTATCTTCAAAGGCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTaaaaaa                                                                   >  1:813310/1‑141 (MQ=255)
                                                                         gCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAAc                                                                  >  1:708253/1‑141 (MQ=255)
                                                                          cAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACg                                                                 <  1:257001/141‑1 (MQ=255)
                                                                            gATGACTGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCCATATCAATATCAGGCATTGTTACCCGTTCTGCGTTTAAATAACGtt                                                               <  2:918893/141‑1 (MQ=255)
                                                                              tGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCa                                                             >  1:1045899/1‑141 (MQ=255)
                                                                                aCCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCaaa                                                           >  2:825998/1‑141 (MQ=255)
                                                                                  cgttatacacatatCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTTTTACACGTTCTGGGTTTAAAAAACGTTCAaata                                                         <  2:856428/128‑1 (MQ=255)
                                                                                        tCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGAt                                                   <  1:200928/141‑1 (MQ=255)
                                                                                        tCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTAAAAAAAAAAAAAAACGTTCAAATAATAGAt                                                   <  2:757682/141‑1 (MQ=255)
                                                                                           caATTCCAGATACATGTAGCTCGCCATATTTTTCTTTGTTTTTCTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGa                                                >  2:797535/1‑141 (MQ=255)
                                                                                           caATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCATTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACTCGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGa                                                <  1:783046/141‑1 (MQ=255)
                                                                                            aaTTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGaa                                               <  2:397952/141‑1 (MQ=255)
                                                                                            aaTTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGaa                                               <  1:236727/141‑1 (MQ=255)
                                                                                             aTTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAAt                                              <  2:588820/141‑1 (MQ=255)
                                                                                               tccatgtACATGTATTTCGCCATATTTTTATTAGACGTACTGACTAACCCTTTCTTGGCGTCTATCTTCAAAGTCAATATCTATATCAGGCATTGTTTCTTGTAAAAAAATAAAAAAACGTTCAAATAATAGATTGAAttt                                            <  2:516565/135‑1 (MQ=255)
                                                                                                  aGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAAt                                         <  2:156206/141‑1 (MQ=255)
                                                                                                   gATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATa                                        >  2:897692/1‑141 (MQ=255)
                                                                                                    aTACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAg                                       >  2:441819/1‑141 (MQ=255)
                                                                                                     tACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAg                                       >  2:255923/1‑141 (MQ=255)
                                                                                                      aCATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGa                                     <  2:688430/141‑1 (MQ=255)
                                                                                                      aCATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGa                                     <  1:755001/141‑1 (MQ=255)
                                                                                                       cATGTAGCTCGCCATATTTTTCTTGGACGTAATGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGNATTNTNACACGTTCTGGGTGTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGAt                                    >  2:1016443/1‑141 (MQ=255)
                                                                                                              cTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGTATCAATCGt                             <  1:953582/141‑1 (MQ=255)
                                                                                                               tCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGtt                            <  2:928970/141‑1 (MQ=255)
                                                                                                                 gCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGt                          >  1:933877/1‑141 (MQ=255)
                                                                                                                   cATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTaa                        >  1:5756/1‑141 (MQ=255)
                                                                                                                   cATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTaa                        >  2:914089/1‑141 (MQ=255)
                                                                                                                   cATATTTTTATTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTaa                        <  2:274935/141‑1 (MQ=255)
                                                                                                                        ttttCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTccc                   >  1:138285/1‑141 (MQ=255)
                                                                                                                        ttttCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTccc                   <  1:629036/141‑1 (MQ=255)
                                                                                                                          ttCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaa                 <  1:347456/141‑1 (MQ=255)
                                                                                                                          ttCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaa                 <  1:650313/141‑1 (MQ=255)
                                                                                                                          ttCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaa                 <  2:56620/141‑1 (MQ=255)
                                                                                                                          ttCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaa                 <  2:569706/141‑1 (MQ=255)
                                                                                                                           tCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaat                >  2:238070/1‑141 (MQ=255)
                                                                                                                           tCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaat                >  1:502160/1‑141 (MQ=255)
                                                                                                                               ggACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCaataaat            <  1:418568/141‑1 (MQ=255)
                                                                                                                                    tACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCAATAAATAACTg       <  1:559707/141‑1 (MQ=255)
                                                                                                                                      cTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCAATAAATCACTAAc     >  2:753300/1‑141 (MQ=255)
                                                                                                                                         aaTGACCCGTGCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCAATAAATAACTGACCAg  <  2:971540/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CTTGAAATTTCATTTAATGTAACTTCATCAAACCCCATAATTCTTCCAACATCTCTAGCAACTGCTCTTGCAAGCAGATGACCGAAAGTCACAATTCCAGATACATGTAGCTCGCCATATTTTTCTTGGACGTACTGAATGACCCTTTCTCGGCGTGTATCTTCAAAGTCAATATCAATATCAGGCATTGTTACACGTTCTGGGTTTAAAAAACGTTCAAATAATAGATTGAATTTAATAGGATCAATCGTTGTAATTCCCAATAAATAACTGACCAG  >  CP000730/1814022‑1814299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: