Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,970,932 A→T 5.2% intergenic (+121/‑612) USA300HOU_1831 → / → USA300HOU_1832 hypothetical protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,970,9320AT 5.2% 319.0 / 5.2 105intergenic (+121/‑612)USA300HOU_1831/USA300HOU_1832hypothetical protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (45/53);  new base T (4/3);  total (49/56)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACTTA  >  CP000730/1970792‑1971072
                                                                                                                                             |                                                                                                                                            
gAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTaa                                                                                                                                              >  2:1033358/1‑141 (MQ=255)
 aaaCGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTaaa                                                                                                                                             >  2:186313/1‑141 (MQ=255)
   aCGAAATTTCTAAGTAGCAGGTTACGTTCTCGTTCACAACTGTTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAAc                                                                                                                                           <  2:180234/141‑1 (MQ=255)
   aCGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAAc                                                                                                                                           <  1:427019/141‑1 (MQ=255)
   aCGAAACGTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAAc                                                                                                                                           <  2:365511/141‑1 (MQ=255)
    cGAAATTTCTAAGTAGCACGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTTAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTATAACt                                                                                                                                          >  1:338895/1‑141 (MQ=255)
      aaaTTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCa                                                                                                                                        >  1:339394/1‑141 (MQ=255)
      aaaTTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCa                                                                                                                                        <  1:1018265/141‑1 (MQ=255)
       aaTTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTGTTTTTTAAAACTCaa                                                                                                                                       >  1:900274/1‑141 (MQ=255)
             tAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAAc                                                                                                                                 >  2:1025130/1‑141 (MQ=255)
               aGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACtt                                                                                                                               >  2:341567/1‑141 (MQ=255)
                     aGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGttt                                                                                                                         <  1:1027473/141‑1 (MQ=255)
                      ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa                                                                                                                        >  1:922924/1‑141 (MQ=255)
                      ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa                                                                                                                        >  1:176913/1‑141 (MQ=255)
                      ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa                                                                                                                        <  2:642984/141‑1 (MQ=255)
                      ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa                                                                                                                        >  1:109371/1‑141 (MQ=255)
                                tcGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAg                                                                                                              >  2:496999/1‑141 (MQ=255)
                                tcGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAg                                                                                                              >  1:22044/1‑141 (MQ=255)
                                 cGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTGTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAgg                                                                                                             >  2:934687/1‑141 (MQ=255)
                                  gATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGa                                                                                                            <  1:767512/141‑1 (MQ=255)
                                     cacaACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGa                                                                                                         >  1:599830/1‑141 (MQ=255)
                                     cacaACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGa                                                                                                         <  2:474114/141‑1 (MQ=255)
                                                 tattaGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAg                                                                                             >  1:400938/1‑141 (MQ=255)
                                                 tattaGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAg                                                                                             <  1:384344/141‑1 (MQ=255)
                                                  attaGTAACAGCATATTTATTTTTTAAAATTAAATACCAAATGAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGt                                                                                            >  1:933982/1‑141 (MQ=255)
                                                    taGTAACAGCATATTTATTTTTTAATATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTTTATTTTTTATGAATTTATTTTTACAAATTATAGTAAGCCATTTTTTGTAGttt                                                                                          >  2:788507/1‑139 (MQ=255)
                                                     aGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGAt                                                                                         >  2:33357/1‑141 (MQ=255)
                                                     aGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGAt                                                                                         >  1:392263/1‑141 (MQ=255)
                                                       tAACAGCATATTTATTTTTTTAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTATAAATCAAATGAACTTATGTTTACAAATTACAGGAATACATCGTTTGTAGTGAttt                                                                                       <  2:340905/141‑1 (MQ=255)
                                                        aaCAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGAttt                                                                                       <  1:326690/141‑1 (MQ=255)
                                                         aCAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTc                                                                                     <  1:186313/141‑1 (MQ=255)
                                                          cAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGAtttaag                                                                                    <  1:1002034/141‑4 (MQ=255)
                                                            gCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCt                                                                                  <  2:139427/141‑1 (MQ=255)
                                                             cATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATCTTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAATCAGACATTGATTGTAGTGAttttgggtt                                                                                 >  2:698522/1‑136 (MQ=255)
                                                                 tttaattTTAAAAAATAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAATTTAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAAt                                                                             >  2:735437/1‑141 (MQ=255)
                                                                     ttttttAAAATTAAATGCCAAATAAACGAGATGACATCAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGcatgaaaaaga                                                                         >  1:784703/1‑131 (MQ=255)
                                                                      tttttAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCatat                                                                        >  1:172642/1‑141 (MQ=255)
                                                                      tttttAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCatat                                                                        >  1:854454/1‑141 (MQ=255)
                                                                         ttttattttaaTGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTa                                                                     >  2:871208/10‑141 (MQ=255)
                                                                          tAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTAt                                                                    >  1:21615/1‑141 (MQ=255)
                                                                           aaaaTTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATg                                                                   >  1:645379/1‑141 (MQ=255)
                                                                           aaaaTTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATg                                                                   >  2:108270/1‑141 (MQ=255)
                                                                             aaTTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAGATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGaa                                                                 <  2:1056976/141‑1 (MQ=255)
                                                                               ttAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAatt                                                               <  1:398694/141‑1 (MQ=255)
                                                                                  aaTGCCGAATAAACGAGATGATATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAAAGTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAattgat                                                            <  2:890869/141‑1 (MQ=255)
                                                                                  aaTGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAattgat                                                            <  2:24432/141‑1 (MQ=255)
                                                                                   aTGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAattgatt                                                           >  1:1074531/1‑141 (MQ=255)
                                                                                   aTGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAattgatt                                                           <  1:723497/141‑1 (MQ=255)
                                                                                        aaaTAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGaaaa                                                      >  1:621703/1‑141 (MQ=255)
                                                                                        aaaTAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGaaaa                                                      <  2:942411/141‑1 (MQ=255)
                                                                                          aTAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACa                                                    <  2:777644/141‑1 (MQ=255)
                                                                                               cGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCt                                               <  2:31345/141‑1 (MQ=255)
                                                                                                gagaTGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCtt                                              >  2:202208/1‑141 (MQ=255)
                                                                                                gagaTGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCtt                                              >  1:793155/1‑141 (MQ=255)
                                                                                                 agaTGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTa                                             <  1:288350/141‑1 (MQ=255)
                                                                                                    tGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGa                                          <  2:176993/141‑1 (MQ=255)
                                                                                                    tGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATAAACTTATTTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGa                                          <  1:972611/141‑1 (MQ=255)
                                                                                                     gACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAACCATTGCTTAGGAt                                         >  2:706796/1‑141 (MQ=255)
                                                                                                     gACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGAt                                         <  2:474383/141‑1 (MQ=255)
                                                                                                     gACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGAt                                         <  2:724858/141‑1 (MQ=255)
                                                                                                      aCATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGAtt                                        <  1:257006/141‑1 (MQ=255)
                                                                                                          tAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCAtt                                    <  1:447110/141‑1 (MQ=255)
                                                                                                             aaaTTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACGTTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATtgtg                                 >  2:699080/1‑141 (MQ=255)
                                                                                                              aaTTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTgtgt                                <  1:322084/141‑1 (MQ=255)
                                                                                                               aTTAGATATTTCTTGTCATCTCTTTTTTTTTTTTTAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGtt                               >  2:216341/1‑141 (MQ=255)
                                                                                                               aTTAGATATTTCTTGTCATCTCTTTTTTATATCTTAAATTAACTTATGATGGCAAATTCTATGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTAAAAACATTACTTAGGATTCATTGTCtt                               >  2:216327/1‑141 (MQ=255)
                                                                                                               aTTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGtt                               <  1:260302/141‑1 (MQ=255)
                                                                                                               aTTAGATATTTCTTGTCAACTCGTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGtt                               <  1:555437/141‑1 (MQ=255)
                                                                                                                     atatTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCt                          <  1:47646/141‑1 (MQ=255)
                                                                                                                       atTTCTTGTCATCTCTTTTTTAAAACTCTAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTg                        >  2:697677/1‑141 (MQ=255)
                                                                                                                        tttCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGc                       <  2:259415/141‑1 (MQ=255)
                                                                                                                        tttCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGc                       <  1:135080/141‑1 (MQ=255)
                                                                                                                         ttCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCa                      <  1:1033358/141‑1 (MQ=255)
                                                                                                                          tCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCAc                     <  2:841068/141‑1 (MQ=255)
                                                                                                                          tCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCAc                     <  2:340717/141‑1 (MQ=255)
                                                                                                                          tCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCAc                     <  2:896368/141‑1 (MQ=255)
                                                                                                                             tGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTCTCCTTGCACttt                  >  1:685821/1‑141 (MQ=255)
                                                                                                                             tGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACttt                  >  1:829549/1‑141 (MQ=255)
                                                                                                                              gTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTg                 <  2:513951/141‑1 (MQ=255)
                                                                                                                              gTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTg                 <  2:514422/141‑1 (MQ=255)
                                                                                                                              gTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTg                 >  1:798408/1‑141 (MQ=255)
                                                                                                                               tCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGa                >  1:659615/1‑141 (MQ=255)
                                                                                                                                cATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGAt               <  2:616233/141‑1 (MQ=255)
                                                                                                                                cATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGAt               <  1:1034021/141‑1 (MQ=255)
                                                                                                                                cATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGAt               <  1:1011298/141‑1 (MQ=255)
                                                                                                                                cATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGAt               >  1:178460/1‑141 (MQ=255)
                                                                                                                                 aTCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGAtt              >  1:378266/1‑141 (MQ=255)
                                                                                                                                     ctTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGc          <  1:1086600/141‑1 (MQ=255)
                                                                                                                                     ctTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGc          <  1:318007/141‑1 (MQ=255)
                                                                                                                                       tttttAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCtt        <  2:359929/141‑1 (MQ=255)
                                                                                                                                       tttttAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCtt        >  1:6037/1‑141 (MQ=255)
                                                                                                                                       tttttAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCtt        >  2:60702/1‑141 (MQ=255)
                                                                                                                                        ttttATAACTCAAATGAACTTATGTTTACAAATTACAGGAATACATCGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCttt       <  2:338895/141‑1 (MQ=255)
                                                                                                                                        ttttAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCttt       >  2:578231/1‑141 (MQ=255)
                                                                                                                                         tttATAACTCAAATGAACTTATGTTTACAAATTACAGGAATACATCGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTa      <  2:94253/141‑1 (MQ=255)
                                                                                                                                           tAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCAGATTTAGTAAGTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACt    >  1:214356/1‑141 (MQ=255)
                                                                                                                                            aaaaCTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACtt   >  1:734637/1‑141 (MQ=255)
                                                                                                                                             aaaCTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACTTa  >  1:595994/1‑141 (MQ=255)
                                                                                                                                             aaaCTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACTTa  <  1:344872/141‑1 (MQ=255)
                                                                                                                                             aaaCTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACTTa  <  2:959343/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTACAAATTATAGGAAGACATTGTTTGTAGTGATTTTCGCTTAAATCATATTTATGAATTGATTGAAAACATTGCTTAGGATTCATTGTGTTATCCTTGCACTTTGATTACGCTTTACTTA  >  CP000730/1970792‑1971072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: