Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,997,013 C→G 5.5% noncoding (61/93 nt) USA300HOU_t0037 ← tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,997,0130CG 5.5% 255.5 / 2.9 83noncoding (61/93 nt)USA300HOU_t0037tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base C (29/49);  new base G (2/3);  total (31/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGCG  >  CP000730/1996874‑1997150
                                                                                                                                           |                                                                                                                                         
cTACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGCGCCCGACGAGCTACCGACCTGCTCCATCCCGCGATAATAAAAACTAATGGCGGAGGAAGAGGCATTCGAACCCCCGCGGcc                                                                                                                                          >  2:94524/1‑141 (MQ=255)
cTACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGcc                                                                                                                                          >  1:311047/1‑141 (MQ=255)
 tACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGccc                                                                                                                                         <  2:413923/141‑1 (MQ=255)
 tACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGccc                                                                                                                                         <  1:79857/141‑1 (MQ=255)
 tACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCACCGCGGccc                                                                                                                                         <  1:255176/141‑1 (MQ=255)
  aCGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCg                                                                                                                                        >  1:235313/1‑141 (MQ=255)
    attaaaaactatgtatTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGtt                                                                                                                                      >  2:652048/14‑141 (MQ=255)
     agaCCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTa                                                                                                                                     >  2:552174/1‑141 (MQ=255)
         cTATAAAATATTGCGGGAGGCGGATTTGAACCCCCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGc                                                                                                                                 <  2:788675/141‑1 (MQ=255)
         cTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGc                                                                                                                                 <  1:247355/141‑1 (MQ=255)
         cTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGc                                                                                                                                 <  2:607808/141‑1 (MQ=255)
          tataAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGcc                                                                                                                                >  1:743008/1‑141 (MQ=255)
          tataAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGcc                                                                                                                                >  2:836417/1‑141 (MQ=255)
            taAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTCAAGGCCCt                                                                                                                              >  2:1032454/1‑141 (MQ=255)
             aaaaTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTg                                                                                                                             <  1:326345/141‑1 (MQ=255)
             aaaaTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTg                                                                                                                             >  1:611747/1‑141 (MQ=255)
                   ttGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtt                                                                                                                       <  1:72584/141‑1 (MQ=255)
                    tGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGttt                                                                                                                      >  1:60018/1‑141 (MQ=255)
                    tGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGACGGctt                                                                                                                      >  1:80145/1‑141 (MQ=255)
                     gCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtttt                                                                                                                     >  1:103548/1‑141 (MQ=255)
                     gCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGtttt                                                                                                                     >  2:435653/1‑141 (MQ=255)
                       gggAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCa                                                                                                                   <  2:931510/141‑1 (MQ=255)
                         gAGGCGGAGTTGAACCACCGGCCTTCGGGTTATGAGCCCGACGAGCCACCGGGCTGCTCCATCCCGCTATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTGAGGCCCTGTCGGTTGTCAAg                                                                                                                 <  2:752298/141‑1 (MQ=37)
                           ggCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGAc                                                                                                               >  2:447394/1‑141 (MQ=255)
                           ggCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCAGCGATACTCCAAAATAATGGCGGAGGCAGAGGGATTCGAACCCCAGCGGCAAGTTAAGGCCCTGTCGGTTTTCAAGAc                                                                                                               <  1:432395/141‑1 (MQ=25)
                                 tttGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                         <  1:810456/141‑1 (MQ=255)
                                 tttGAACCACCGACCTTCATCGGGTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                         <  1:826289/141‑1 (MQ=37)
                                 ttggAACCACCCACCTTCGGGTTAGGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                         <  2:650327/138‑1 (MQ=255)
                                   tGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCt                                                                                                       <  1:654525/141‑1 (MQ=255)
                                    gAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGCCCATGTCGGTTTTCAAGACCGATCCCtt                                                                                                      >  2:1018390/1‑141 (MQ=255)
                                        caccGCCCTTCGGTTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCTCGATAATAAAAAATAATGGCGGAGGAAGTGGTGTTCGAACCCCCGCTGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGc                                                                                                  <  2:38021/141‑1 (MQ=37)
                                        caccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                         <  1:116370/134‑1 (MQ=255)
                                        caccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATcc                                                                                                         >  2:116370/1‑134 (MQ=255)
                                        caccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGc                                                                                                  <  2:878613/141‑1 (MQ=255)
                                        caccGACCCCCGGGTTACGAGCCCGACGGGCTACCGAACTGCCCCATCCCGCGATAGTACAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGc                                                                                                  <  2:60018/141‑1 (MQ=18)
                                           cGACCGTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTACAAACAGCAATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCgg                                                                                               <  2:493725/141‑1 (MQ=25)
                                             aCCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGAc                                                                                             <  1:250867/141‑1 (MQ=255)
                                             aCCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGAc                                                                                             >  2:558100/1‑141 (MQ=255)
                                              ccTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACt                                                                                            <  1:327367/141‑1 (MQ=255)
                                                ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCTATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTg                                                                                          <  1:556841/141‑1 (MQ=255)
                                                ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATACAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTg                                                                                          >  2:826282/1‑141 (MQ=255)
                                                ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTg                                                                                          <  2:288749/141‑1 (MQ=255)
                                                  cGGGGTATGAGCCCGACGAGCTACCGAACTGCGCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCACGACCGATCCCTTCAGCCGGACTTggg                                                                                        <  2:159204/141‑1 (MQ=255)
                                                    ggTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTa                                                                                      <  2:178279/141‑1 (MQ=255)
                                                       tATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtc                                                                                   <  2:819647/141‑1 (MQ=255)
                                                        aTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCCGCCGGACTTGGGTATtcc                                                                                  >  1:1045828/1‑141 (MQ=255)
                                                        aTGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcc                                                                                  <  2:1030110/141‑1 (MQ=255)
                                                         tGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcct                                                                                 >  2:933038/1‑141 (MQ=255)
                                                                gacgaGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCattat                                                                          <  1:755577/141‑1 (MQ=255)
                                                                   gaGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGAAAtccgacgagactcg                                                                       >  2:741056/1‑130 (MQ=21)
                                                                         ccGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATtatatggac                                                                 <  1:558100/141‑6 (MQ=14)
                                                                           gAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAAt                                                               <  1:829734/141‑1 (MQ=255)
                                                                                gCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTc                                                                                                                           <  2:605526/76‑1 (MQ=17)
                                                                                gCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTc                                                                                                                           >  1:605526/1‑76 (MQ=17)
                                                                                 cTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATATGTACCTCGcagg                                                         >  2:647338/1‑138 (MQ=255)
                                                                                 cTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTat                                                         <  1:737741/141‑1 (MQ=255)
                                                                                   ccttCCCGCGATAAGAAAAAGTGTTGGCGGAGCGTGAGGGGTGGGAGGCCGTGGTGCCGGTGAATGGCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTatta                                                       <  1:99129/138‑1 (MQ=12)
                                                                                   ccATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTatca                                                       <  2:763342/141‑3 (MQ=255)
                                                                                     aTCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTattaat                                                     <  1:771383/141‑1 (MQ=255)
                                                                                     aTCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTattaat                                                     >  1:152218/1‑141 (MQ=255)
                                                                                          gcgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaa                                                >  1:568578/1‑141 (MQ=255)
                                                                                          gcgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaa                                                >  1:568700/1‑141 (MQ=255)
                                                                                          gcgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATAATTATATGTAACTCGCTattcactaacc                                                >  2:488436/1‑133 (MQ=18)
                                                                                           cgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaaa                                               <  2:72924/141‑1 (MQ=255)
                                                                                           cgATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATaaa                                               >  1:258015/1‑141 (MQ=255)
                                                                                             ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                             <  2:139947/141‑1 (MQ=255)
                                                                                             ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                             <  1:720807/141‑1 (MQ=255)
                                                                                             ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                             <  2:856823/141‑1 (MQ=255)
                                                                                             ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                             <  2:743008/141‑1 (MQ=255)
                                                                                             ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                             <  2:638053/141‑1 (MQ=255)
                                                                                             ataataAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAAt                                             <  2:960054/141‑1 (MQ=255)
                                                                                                 taaAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTaaa                                         <  1:622072/141‑1 (MQ=255)
                                                                                                      aactaaggCGGTGGAAGAGCGATTCGAGCCACGGCGGCCCGGTAAGGCCCTGTCGGGTTGCAAGACCGATCCCATCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAGATTAAATGGCg                                    <  2:40795/135‑1 (MQ=21)
                                                                                                       ataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCgg                                   <  1:892095/141‑1 (MQ=255)
                                                                                                       ataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCgg                                   <  1:547303/141‑1 (MQ=255)
                                                                                                       ataatGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGCTTTTTTTTACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTGTAGGTAAATCGCTATTAATTATAAAATTAAATGGCgg                                   <  1:782614/141‑1 (MQ=25)
                                                                                                           tGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGtctc                               <  1:230136/141‑1 (MQ=255)
                                                                                                           ggtcGGAGGAAGAGGGATTCGACCGCCCGCGGCCCGTTAAGGCCCTGGCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGtctc                               <  1:274238/138‑1 (MQ=255)
                                                                                                            ggCGGAGGAAGAGGGATTCGAGCCCCCGCGGCCCGGTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCg                              <  1:524359/141‑1 (MQ=255)
                                                                                                                gaggaAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACgg                          <  1:382673/141‑1 (MQ=255)
                                                                                                                           aTTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTAATATAGGTAAATAGACCCTAAGAATAAAATTAAATGGCGGTCTCGACGGGAATCGAAccc               <  1:520611/141‑1 (MQ=21)
                                                                                                                            ttCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGTCGGGAATCGAACCcg              >  1:899916/1‑141 (MQ=255)
                                                                                                                                aaCCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGAt          >  1:622405/1‑141 (MQ=255)
                                                                                                                                  cccccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAATATTATATGTAAATCGCTATTCTCGATCCAGCTACATGACGGTCTCCACGGGAATCGCACCCACGAtca        >  2:962950/1‑140 (MQ=17)
                                                                                                                                    cccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTcc      >  1:425512/1‑141 (MQ=255)
                                                                                                                                    cccGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTcc      <  2:611747/141‑1 (MQ=255)
                                                                                                                                       gcgGCCCGTTAAGGCCCTATCGTGTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGc   <  1:826282/141‑1 (MQ=255)
                                                                                                                                        cgGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGCg  >  1:590995/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CTACGAGACCTATAAAATATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGATAATAAAAAATAATGGCGGAGGAAGAGGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCATTATTATAGGTAAATCGCTATTAATTATAAAATTAAATGGCGGTCTCGACGGGAATCGAACCCGCGATCTCCTGCG  >  CP000730/1996874‑1997150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: