Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,091,517 A→G 5.9% N28N (AAT→AAC USA300HOU_1954 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,091,5170AG 5.9% 292.9 / 7.4 102N28N (AAT→AACUSA300HOU_1954hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base A (38/57);  new base G (2/4);  total (41/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 104 total aligned reads displayed.

CACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAGCTTT  >  CP000730/2091377‑2091657
                                                                                                                                            |                                                                                                                                            
cacaTTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCa                                                                                                                                              >  2:756291/1‑141 (MQ=255)
 acaTTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCAt                                                                                                                                             >  1:566419/1‑141 (MQ=255)
    ttttCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGTTTTTTTGACCTAATTTAATTTCATTGa                                                                                                                                          <  1:880916/141‑1 (MQ=255)
    ttttCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGa                                                                                                                                          <  1:1066537/141‑1 (MQ=255)
    ttttCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATCTCATTGa                                                                                                                                          <  1:858411/141‑1 (MQ=255)
        cATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAgtgt                                                                                                                                      >  2:888648/1‑141 (MQ=255)
        cATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAgtgt                                                                                                                                      >  1:681005/1‑141 (MQ=255)
          tatCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTCATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGc                                                                                                                                    <  2:376568/141‑1 (MQ=255)
          tatCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGc                                                                                                                                    >  2:671357/1‑141 (MQ=255)
               cGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGc                                                                                                                               <  2:74857/141‑1 (MQ=255)
               cGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGc                                                                                                                               <  1:113746/141‑1 (MQ=255)
               cGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGc                                                                                                                               <  2:645106/141‑1 (MQ=255)
                 tATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCAt                                                                                                                             <  1:146872/141‑1 (MQ=255)
                 tATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCAt                                                                                                                             <  2:218947/141‑1 (MQ=255)
                 tATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCAt                                                                                                                             <  1:665262/141‑1 (MQ=255)
                 tATGTTCTTATCATTTTCTTTCTTATTCTGTTCATCTATCTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCAt                                                                                                                             <  1:232078/141‑1 (MQ=255)
                      tCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGttt                                                                                                                        <  2:703101/141‑1 (MQ=255)
                       cTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGtttt                                                                                                                       >  2:105708/1‑141 (MQ=255)
                        ttATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTa                                                                                                                      <  1:410375/141‑1 (MQ=255)
                        ttATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTa                                                                                                                      <  1:715691/141‑1 (MQ=255)
                          aTCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTAtt                                                                                                                    >  2:442316/1‑140 (MQ=255)
                             tttttCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCAtt                                                                                                                 <  1:987531/140‑1 (MQ=255)
                             aTTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCAtt                                                                                                                 <  1:719064/141‑1 (MQ=255)
                              ttttCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATtc                                                                                                                <  1:353180/141‑1 (MQ=255)
                               tttctttcTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATtct                                                                                                               >  2:284458/1‑141 (MQ=255)
                                 tctttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTctct                                                                                                             <  1:671357/141‑1 (MQ=255)
                                  ctttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCtctt                                                                                                            >  1:351707/1‑141 (MQ=255)
                                  ctttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCtctt                                                                                                            <  2:579354/141‑1 (MQ=255)
                                     tcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTa                                                                                                         <  1:937157/141‑1 (MQ=255)
                                      cttATTCTTTTCATCTATTTCTCTTTCTTTTTTATTTTCTTTTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGATCTAATTTAATTTCATTGAGTGTGCTTTGTATTGTTTTATTATTCTCTTTTaa                                                                                                        >  2:448365/1‑141 (MQ=255)
                                      cttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCCTTCTCTTCTaa                                                                                                        >  2:448432/1‑141 (MQ=255)
                                         aTTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCt                                                                                                     <  2:16487/141‑1 (MQ=255)
                                           tCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTc                                                                                                   >  2:1085898/1‑141 (MQ=255)
                                            cTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTTTTTTATTTTTATTTCATTGAGTGTGCTGTGCATTGTTTTATCCTTCTCTTCTCACCTTCt                                                                                                  >  2:249030/1‑141 (MQ=255)
                                            cTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCt                                                                                                  >  1:958251/1‑141 (MQ=255)
                                               ttCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGAt                                                                                               <  2:664700/141‑1 (MQ=255)
                                                tCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTTTTTTATCATTCTCTTCTAACCTTCTGATg                                                                                              >  2:170599/1‑141 (MQ=255)
                                                 cATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATgc                                                                                             >  1:892369/1‑141 (MQ=255)
                                                   tCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATgcgc                                                                                           <  2:1025094/141‑1 (MQ=255)
                                                   tCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATgcgc                                                                                           >  1:674997/1‑141 (MQ=255)
                                                   tCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATgcgc                                                                                           <  2:10268/141‑1 (MQ=255)
                                                    cTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGcc                                                                                          <  2:402938/141‑1 (MQ=255)
                                                     tATTTCCCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCa                                                                                         <  1:331349/141‑1 (MQ=255)
                                                      aTTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTTTCTTCTAACCTTCTGATGCGCCAt                                                                                        >  2:642796/1‑141 (MQ=255)
                                                      aTTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCAt                                                                                        <  1:851007/141‑1 (MQ=255)
                                                      aTTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCAt                                                                                        >  1:550899/1‑141 (MQ=255)
                                                       tttCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCAtt                                                                                       >  1:444599/1‑141 (MQ=255)
                                                       tttCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCAtt                                                                                       >  1:871406/1‑141 (MQ=255)
                                                               tctttTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCg                                                                               <  2:398333/141‑1 (MQ=255)
                                                                ctttTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCgt                                                                              <  2:427376/141‑1 (MQ=255)
                                                                      gAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGttttt                                                                        >  2:515137/1‑141 (MQ=255)
                                                                       aaTAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGtttttt                                                                       <  1:58083/141‑1 (MQ=255)
                                                                       aaTAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGtttttt                                                                       <  1:674581/141‑1 (MQ=255)
                                                                         ttttttttAAGGTTTGATATAATTTAATGTTAACTTGCTCTTGGGTTTTTTGTCCTAATTTAATTTCCTTGAGTTTGTTTTGCTTTGTTTTTTCATTCTCTTCTAACCTTCTGATGCTTAATTCATTTTCGTGTTTTTTGa                                                                     >  2:333067/8‑141 (MQ=255)
                                                                          aGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTTTTTTTTTTTTATTTCATTGAGTGTGCTTAGCATTGTTTTATCTTTCTCTTCTGACCTTCTGATGCGCCATTCCTGTTCGTTTTTTTTGat                                                                    >  2:912707/1‑140 (MQ=255)
                                                                          aGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGaa                                                                    <  2:979575/141‑1 (MQ=255)
                                                                           ttttttAAGGTTTTATCGAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTACTTTCATTGTGTTTTTTTTTTTTTGTTTTTTCATTTTCTTCTAACCTTCTGATTCGCCTTTCATGTTCGTGTTTCTTGatc                                                                   >  2:885961/6‑139 (MQ=255)
                                                                            cATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAacc                                                                  <  2:324806/141‑1 (MQ=255)
                                                                            cATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAacc                                                                  <  1:596245/141‑1 (MQ=255)
                                                                                tAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCAccc                                                              <  1:118401/141‑1 (MQ=255)
                                                                                   ggTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCaa                                                           >  2:521910/1‑141 (MQ=255)
                                                                                     ttttATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAAtt                                                         >  1:959880/1‑141 (MQ=255)
                                                                                       ttATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCa                                                       >  2:674259/1‑141 (MQ=255)
                                                                                        tATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTTTTTTTTTTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACTCAAATTCAg                                                      >  2:217017/1‑141 (MQ=255)
                                                                                        tATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAg                                                      >  1:638182/1‑141 (MQ=255)
                                                                                         aTCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGt                                                     >  2:688731/1‑141 (MQ=255)
                                                                                         aTCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCAATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGt                                                     >  1:927796/1‑141 (MQ=255)
                                                                                             aaTTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTacac                                                 >  1:197467/1‑141 (MQ=255)
                                                                                             aaTTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTacac                                                 <  1:668694/141‑1 (MQ=255)
                                                                                              aTTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACAcc                                                >  1:912891/1‑141 (MQ=255)
                                                                                                 tAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGc                                             <  2:696756/141‑1 (MQ=255)
                                                                                                  aaTGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATATTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCt                                            >  1:803134/1‑141 (MQ=255)
                                                                                                    tGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCttt                                          <  2:444599/141‑1 (MQ=255)
                                                                                                    tGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCttt                                          <  2:871406/141‑1 (MQ=255)
                                                                                                    tGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCttt                                          <  1:284458/141‑1 (MQ=255)
                                                                                                     gTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTc                                         <  2:170260/141‑1 (MQ=255)
                                                                                                      ttAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCt                                        <  2:111325/141‑1 (MQ=255)
                                                                                                        aaCTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTaa                                      <  2:768706/141‑1 (MQ=255)
                                                                                                        aaCTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTaa                                      <  2:237813/141‑1 (MQ=255)
                                                                                                             gCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGaa                                 <  1:415020/141‑1 (MQ=255)
                                                                                                                ctTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATaa                              <  2:630700/141‑1 (MQ=255)
                                                                                                                ctTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATaa                              <  1:756817/141‑1 (MQ=255)
                                                                                                                    ggTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGAt                          >  1:370228/1‑141 (MQ=255)
                                                                                                                    ggTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGAt                          <  1:473165/141‑1 (MQ=255)
                                                                                                                         tttGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTGCGTGGTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGa                     >  1:836208/1‑141 (MQ=255)
                                                                                                                              ccTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATc                <  1:599661/141‑1 (MQ=255)
                                                                                                                              ccTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATc                <  2:139983/141‑1 (MQ=255)
                                                                                                                               cTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCt               <  1:521910/141‑1 (MQ=255)
                                                                                                                                   tttaatttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGttagatact           <  1:685329/141‑9 (MQ=255)
                                                                                                                                    ttaatttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTc          <  2:452611/141‑1 (MQ=255)
                                                                                                                                      aatttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCAt        <  1:472800/141‑1 (MQ=255)
                                                                                                                                       atttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATa       >  1:439517/1‑141 (MQ=255)
                                                                                                                                        tttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTTGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAGAAGAATAAAGATTATGAGTATCTAACTCATAg      >  2:843380/1‑141 (MQ=255)
                                                                                                                                        tttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAg      <  2:566419/141‑1 (MQ=255)
                                                                                                                                        tttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAg      <  1:533136/141‑1 (MQ=255)
                                                                                                                                        tttCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAg      >  2:967432/1‑141 (MQ=255)
                                                                                                                                         ttCATTGAGTGTGATGATCAGTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCCTGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAGc     <  1:333067/141‑1 (MQ=255)
                                                                                                                                          tCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAGCt    >  2:927024/1‑141 (MQ=255)
                                                                                                                                           cATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAGCtt   <  1:870129/141‑1 (MQ=255)
                                                                                                                                            aTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAGCttt  >  2:904877/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTATCTAACTCATAGCTTT  >  CP000730/2091377‑2091657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: