Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,119,322 T→A 5.3% V308V (GTA→GTT USA300HOU_1992 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,119,3220TA 5.3% 336.8 / 7.0 106V308V (GTA→GTTUSA300HOU_1992hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/58);  new base A (3/3);  total (45/61)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCTTACC  >  CP000730/2119182‑2119462
                                                                                                                                            |                                                                                                                                            
tcaAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCt                                                                                                                                              >  1:494269/1‑141 (MQ=255)
 caAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTTATGTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTa                                                                                                                                             >  1:475871/1‑141 (MQ=255)
  aaGATATTCAAATTTTTGATTTTTAGCTTTTTCTTAATCACTTTTTAATCCTTTATGATTTTCTAATGATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAc                                                                                                                                            >  2:385820/1‑141 (MQ=255)
   aGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAcc                                                                                                                                           <  2:4350/141‑1 (MQ=255)
   aGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAcc                                                                                                                                           >  1:42230/1‑141 (MQ=255)
    gATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCg                                                                                                                                          <  1:705834/141‑1 (MQ=255)
     atatTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGt                                                                                                                                         <  2:1035694/141‑1 (MQ=255)
        ttCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                      <  2:612590/141‑1 (MQ=255)
        ttCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                      <  2:773443/141‑1 (MQ=255)
        ttCAAATTTTTGATTTTTAGCTTTTTCTAAATAACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                      <  2:460475/141‑1 (MQ=255)
         tCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCa                                                                                                                                     >  1:890972/1‑141 (MQ=255)
           aaaTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAtt                                                                                                                                   >  2:679596/1‑141 (MQ=255)
             aTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAtttt                                                                                                                                 >  1:993157/1‑141 (MQ=255)
                  tGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACg                                                                                                                            >  1:231097/1‑141 (MQ=255)
                   gATTTTTTTTTTTTTTTTAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCTTGATTTTTTGTTTTTTTTGTTTTTTTTTTTTTTTAAGTTTTGCTACCGTTCCATTTTCAACgt                                                                                                                           >  2:190178/1‑141 (MQ=255)
                   gATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                                           <  1:761685/141‑1 (MQ=255)
                     tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgtgt                                                                                                                         <  2:404610/141‑1 (MQ=255)
                         tAGCTTTTTCTAAATCACTTTTTAATCCTTTATGGTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAAtt                                                                                                                     >  1:959434/1‑141 (MQ=255)
                         tAGCTTTTTCTAAAGCACTTTTTAATCCTTTATGATTGTCTAGTGAGGGATTACTTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCATCGGGTaagt                                                                                                                     >  2:648129/1‑139 (MQ=255)
                           gCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCa                                                                                                                   <  1:991182/141‑1 (MQ=255)
                             tttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAtt                                                                                                                 >  2:558833/1‑141 (MQ=255)
                               tttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTg                                                                                                               <  1:989453/141‑1 (MQ=255)
                                      aTCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGc                                                                                                        >  1:342366/1‑141 (MQ=255)
                                      aTCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGc                                                                                                        <  2:144585/141‑1 (MQ=255)
                                       tCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCa                                                                                                       <  1:515907/141‑1 (MQ=255)
                                         acgttttATTCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                                     <  2:684777/138‑1 (MQ=255)
                                         aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                                     <  2:1040893/141‑1 (MQ=255)
                                         aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                                     <  2:1040930/141‑1 (MQ=255)
                                               tAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAtttt                                                                                               <  2:1017113/141‑1 (MQ=255)
                                                tttttttttttaTTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAATTTTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAATCATGTAttttt                                                                                              >  2:520088/12‑140 (MQ=255)
                                                aaTCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTg                                                                                              >  2:450697/1‑141 (MQ=255)
                                                 aTCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGt                                                                                             <  2:37001/141‑1 (MQ=255)
                                                   ccTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTtc                                                                                           <  1:358278/141‑1 (MQ=255)
                                                    cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                                                                          >  1:532003/1‑141 (MQ=255)
                                                    cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATCTGTTGTTTGTTTTGCTAGAATCTGGTTTTAACGTGTGCTACCGTGCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                                                                          >  1:44367/1‑141 (MQ=255)
                                                       tATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCAt                                                                                       <  2:562083/141‑1 (MQ=255)
                                                           aTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACt                                                                                   <  1:307713/141‑1 (MQ=255)
                                                            ttttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACtt                                                                                  <  2:759539/141‑1 (MQ=255)
                                                             tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                                                                 >  2:756916/1‑141 (MQ=255)
                                                             tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                                                                 >  1:635075/1‑141 (MQ=255)
                                                              ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                                                                <  1:575016/141‑1 (MQ=255)
                                                              ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                                                                <  1:513899/141‑1 (MQ=255)
                                                              ttCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACtggc                                                                                <  1:779690/141‑4 (MQ=255)
                                                                   ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                                           >  1:212107/1‑141 (MQ=255)
                                                                        tGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTg                                                                      <  2:311452/141‑1 (MQ=255)
                                                                        tGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTg                                                                      <  2:386621/141‑1 (MQ=255)
                                                                          aTTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtc                                                                    <  1:105298/141‑1 (MQ=255)
                                                                           ttACGTCTATTTTCTTCATGTGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                                                                   <  2:139559/141‑1 (MQ=255)
                                                                           ttACGTCTATTTTCTTCATGTGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                                                                   <  2:139581/141‑1 (MQ=255)
                                                                           ttACGTCTATTTTCTTCATGTGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTAGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                                                                   <  2:575017/141‑1 (MQ=255)
                                                                           ttACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                                                                   >  1:285571/1‑141 (MQ=255)
                                                                           ttACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                                                                   <  2:638909/141‑1 (MQ=255)
                                                                                   aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg                                                           <  1:735695/141‑1 (MQ=255)
                                                                                   aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg                                                           >  2:797661/1‑141 (MQ=255)
                                                                                    ttttCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGt                                                          <  2:184876/141‑1 (MQ=255)
                                                                                     tttCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCgct                                                         <  2:89043/141‑3 (MQ=255)
                                                                                     tttCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGtt                                                         <  1:730200/141‑1 (MQ=255)
                                                                                        tttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCa                                                      <  2:450862/140‑1 (MQ=255)
                                                                                        cttcATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCa                                                      >  2:126603/1‑141 (MQ=255)
                                                                                        cttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCa                                                      >  2:1044024/1‑141 (MQ=255)
                                                                                        cttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCa                                                      <  1:705960/141‑1 (MQ=255)
                                                                                          tcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAt                                                    >  2:613865/1‑141 (MQ=255)
                                                                                          tcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAt                                                    <  1:1011187/141‑1 (MQ=255)
                                                                                           cATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAtt                                                   <  2:890972/141‑1 (MQ=255)
                                                                                             tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTTTAATTCATTTGTTTAAGCATGGATTTTGTTCTCATTACTTACTCTGTTCTTGTTTTCTATTTGTTTCAAtttt                                                 >  2:487311/1‑139 (MQ=255)
                                                                                             tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCt                                                 <  2:1081236/141‑1 (MQ=255)
                                                                                             tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCt                                                 <  1:196663/141‑1 (MQ=255)
                                                                                               cgttaTTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGa                                               <  1:421213/137‑1 (MQ=255)
                                                                                               cGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGa                                               <  1:207649/141‑1 (MQ=255)
                                                                                               cGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGa                                               <  1:207681/141‑1 (MQ=255)
                                                                                                gTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGAt                                              <  1:6555/141‑1 (MQ=255)
                                                                                                   aTTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGt                                           <  1:132188/141‑1 (MQ=255)
                                                                                                       gttgttTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTAt                                       <  2:1046794/141‑1 (MQ=255)
                                                                                                       gttgttTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTACATTTTCAACGTGTAATTCATTTGTTAAAGAATGGCTTTTGTTGTCATTTCTTGCGCAGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTAt                                       >  1:157947/1‑141 (MQ=255)
                                                                                                            ttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTTATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCt                                  >  2:172862/1‑141 (MQ=255)
                                                                                                            ttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCt                                  <  2:793125/141‑1 (MQ=255)
                                                                                                             tgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTa                                 >  1:606299/1‑141 (MQ=255)
                                                                                                               ttttGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAAt                               <  2:189716/141‑1 (MQ=255)
                                                                                                                   gCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGAt                           >  1:780429/1‑141 (MQ=255)
                                                                                                                       tAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACg                       <  1:290545/141‑1 (MQ=255)
                                                                                                                            tcGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaat                  >  2:296440/1‑141 (MQ=255)
                                                                                                                            tcGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaat                  <  2:331390/141‑1 (MQ=255)
                                                                                                                              gTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatta                >  1:911137/1‑141 (MQ=255)
                                                                                                                              gTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatta                >  1:464016/1‑141 (MQ=255)
                                                                                                                              gTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatta                >  2:621379/1‑141 (MQ=255)
                                                                                                                              gTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTGGTTACGCAaatta                >  2:865330/1‑141 (MQ=255)
                                                                                                                               ttttAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaattaa               <  2:863366/141‑1 (MQ=255)
                                                                                                                                tttAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaattaat              >  2:608895/1‑141 (MQ=255)
                                                                                                                                 ttAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAcattaatt             <  2:286099/141‑1 (MQ=255)
                                                                                                                                  tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt            >  1:957547/1‑141 (MQ=255)
                                                                                                                                   aaGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttc           <  2:570654/141‑1 (MQ=255)
                                                                                                                                   aaGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttc           >  2:180615/1‑141 (MQ=255)
                                                                                                                                   aaGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttc           >  1:176252/1‑141 (MQ=255)
                                                                                                                                   aaGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttc           >  2:463436/1‑141 (MQ=255)
                                                                                                                                        ttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGTTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCt      >  2:633348/1‑141 (MQ=255)
                                                                                                                                        ttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCt      <  1:766900/141‑1 (MQ=255)
                                                                                                                                        ttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCt      <  2:212107/141‑1 (MQ=255)
                                                                                                                                          gCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCTTa    <  1:356409/141‑1 (MQ=255)
                                                                                                                                          gCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCTTa    <  2:823906/141‑1 (MQ=255)
                                                                                                                                            tACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCTTAcc  >  2:532056/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCTTACC  >  CP000730/2119182‑2119462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: