Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,292,077 G→A 12.4% intergenic (+63/‑73) USA300HOU_2155 → / → USA300HOU_2156 transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,292,0770GA12.4% 322.2 / 26.1 120intergenic (+63/‑73)USA300HOU_2155/USA300HOU_2156transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (54/51);  new base A (8/7);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 121 total aligned reads displayed.

ATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCA  >  CP000730/2291941‑2292217
                                                                                                                                        |                                                                                                                                             
aTACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACa                                                                                                                                           >  1:69336/1‑141 (MQ=32)
aTACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACa                                                                                                                                           <  2:486747/141‑1 (MQ=32)
  aCAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACaaa                                                                                                                                         >  2:741799/1‑141 (MQ=32)
    aaTTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAgg                                                                                                                                       <  1:403014/141‑1 (MQ=32)
      tttATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGgag                                                                                                                                     <  2:917102/141‑1 (MQ=32)
      tgtatgaatcatcATTTAATGATGGGCCTATAGAAGTAATTAATAATAGGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAAGTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGgag                                                                                                                                     <  2:638630/129‑1 (MQ=11)
       ttattaCCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGgaga                                                                                                                                    >  1:57637/1‑141 (MQ=32)
       ttattaCCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGgaga                                                                                                                                    >  1:711/1‑141 (MQ=32)
                aGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAaagaa                                                                                                                           <  1:447131/141‑1 (MQ=35)
                       aataatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAAcc                                                                                                                    >  1:509326/1‑141 (MQ=38)
                       aataatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAAcc                                                                                                                    >  2:735314/1‑141 (MQ=38)
                        ataatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCa                                                                                                                   >  1:739304/1‑141 (MQ=38)
                         taatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCaa                                                                                                                  <  2:400721/141‑1 (MQ=37)
                          aatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAg                                                                                                                 <  2:419514/141‑1 (MQ=37)
                          aatGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAg                                                                                                                 <  2:1034724/141‑1 (MQ=37)
                           atGGTGCTATAGAAGGAATTAATAATAAGATAGAATTAATCAAGAGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGATTATACAAAGGAGAAAAAAAGAAGACAACCATGc                                                                                                                <  2:839479/141‑1 (MQ=11)
                             ggTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGccc                                                                                                              >  2:896474/1‑141 (MQ=38)
                             ggTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGccc                                                                                                              <  1:777653/141‑1 (MQ=38)
                                 cTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCaata                                                                                                          <  2:50700/141‑1 (MQ=38)
                                  tataGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCaataa                                                                                                         <  2:26340/141‑1 (MQ=38)
                                     aGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATgg                                                                                                      <  1:834350/141‑1 (MQ=38)
                                      gAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGa                                                                                                     >  1:690739/1‑141 (MQ=38)
                                       aaGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGAc                                                                                                    >  1:185379/1‑141 (MQ=38)
                                         ggAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTAAAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTg                                                                                                  <  1:16162/141‑1 (MQ=37)
                                            aTTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGgcc                                                                                               >  1:888344/1‑141 (MQ=38)
                                            aTTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTAGTACAAAGGAGAAAAAAAGAAGACAACCAAGCACAATACTGGACTGgca                                                                                               >  2:1024194/1‑140 (MQ=255)
                                             ttAATAATAAGATAAAATTAATCAAGTGAATTTCTTTGGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGgccg                                                                                              >  1:857437/1‑141 (MQ=37)
                                              taataataaGATAAAATTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGgccgc                                                                                             >  1:480932/1‑141 (MQ=9)
                                                ataataaGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCt                                                                                           >  1:460609/1‑141 (MQ=38)
                                                ataataaGATAAAATTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGATTGGCCGCCt                                                                                           <  1:816025/141‑1 (MQ=12)
                                                   ataaGATAAAATTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCtaat                                                                                        >  2:419402/1‑141 (MQ=9)
                                                    aaaGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCtaata                                                                                       <  2:603779/140‑1 (MQ=37)
                                                      aGATAAAATTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCtaataat                                                                                     >  1:1005605/1‑141 (MQ=17)
                                                          aaaaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATaaaa                                                                                 <  2:201443/141‑1 (MQ=37)
                                                           aaaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAg                                                                                <  1:1023405/141‑1 (MQ=38)
                                                           aaaTTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCtaataa                                                                                      <  1:177376/135‑1 (MQ=12)
                                                           aaaTTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCtaataa                                                                                      >  2:177376/1‑135 (MQ=9)
                                                           aaaTTAATCAAGCGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATaaaaa                                                                                >  1:674568/1‑140 (MQ=2)
                                                            aaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGc                                                                               >  1:880023/1‑141 (MQ=39)
                                                            aaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGc                                                                               <  1:602292/141‑1 (MQ=39)
                                                             atttaccAAGTGAATTTCTTTTGGTTACCCACATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGCGAAAAACAGAAGCCACCCCACCCCAATAATGGACTGGCCGCCTAATAATAAAAGct                                                                              <  2:270318/135‑1 (MQ=11)
                                                             aTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGct                                                                              <  2:21701/141‑1 (MQ=40)
                                                                aaTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTa                                                                           <  2:557344/141‑1 (MQ=40)
                                                                aaTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACCTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTa                                                                           <  1:301327/141‑1 (MQ=255)
                                                                 aTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTaa                                                                          >  1:111452/1‑141 (MQ=40)
                                                                 aTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTaa                                                                          <  2:1070811/141‑1 (MQ=40)
                                                                   cAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTaaaa                                                                        >  2:68875/1‑141 (MQ=40)
                                                                      gTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGtt                                                                     >  1:870118/1‑141 (MQ=39)
                                                                      gTGAAGTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGtt                                                                     <  1:513/141‑1 (MQ=255)
                                                                       tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                    <  1:458787/141‑1 (MQ=38)
                                                                       tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                    <  1:1056287/141‑1 (MQ=38)
                                                                       tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                    >  1:35081/1‑141 (MQ=38)
                                                                       cgAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTg                                                                    >  1:212360/2‑141 (MQ=2)
                                                                        gAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGt                                                                   <  1:13767/141‑1 (MQ=38)
                                                                          aTTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTAt                                                                 <  2:42462/141‑1 (MQ=38)
                                                                           tttCTCTTGGTTACAGAAATTTCAACAAATTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAACGCTCTCAAAGTTGTAtt                                                                >  2:247740/1‑141 (MQ=255)
                                                                             tctcTTGGTTAGAGAAATTGCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGCTGTAtttt                                                              >  2:496492/1‑141 (MQ=255)
                                                                              cTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTa                                                            >  1:686434/1‑141 (MQ=38)
                                                                               ttttGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaa                                                           >  1:192292/1‑141 (MQ=38)
                                                                               ttttGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaa                                                           >  1:493358/1‑141 (MQ=37)
                                                                                tgtGGTTACAGAAATTTCAACAACTATAAAGCACGTATAATGATGATGTTCAGCATGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaaa                                                          <  2:756584/139‑1 (MQ=255)
                                                                                 ttGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaaaa                                                         >  1:866925/1‑141 (MQ=38)
                                                                                    gTTACAGAAATTTCAACAACTTTAAAGCATGTATCATAATGATTTTCAGCTTATACAAAGAAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTTaaaatag                                                      <  2:442948/141‑4 (MQ=11)
                                                                                     ttACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAg                                                     <  2:473054/141‑1 (MQ=38)
                                                                                      tACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGt                                                    <  1:393222/141‑1 (MQ=38)
                                                                                       aCAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGtt                                                   >  1:1042477/1‑141 (MQ=38)
                                                                                           aaaTTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCttt                                               >  1:249910/1‑141 (MQ=38)
                                                                                              tttCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTaaa                                            >  1:762042/1‑141 (MQ=38)
                                                                                               ttCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAAt                                           <  1:495051/141‑1 (MQ=38)
                                                                                                tCAACAACTGTAAAGCACGTATAATGATGATTGTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAAtt                                          <  2:216970/141‑1 (MQ=255)
                                                                                                  aacaacTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATCAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATtat                                        >  1:853438/1‑141 (MQ=38)
                                                                                                  aacaacTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATtat                                        >  1:315480/1‑141 (MQ=38)
                                                                                                  aacaacTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATtat                                        >  2:748289/1‑141 (MQ=38)
                                                                                                      acTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAATAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCACTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATAc                                    >  2:795363/1‑141 (MQ=255)
                                                                                                       cTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGCGAAAAAAAGAAGACAACCACGCCCAATAATGGACTGGCAGCCTAATAATAAAAGCACTAAAAGTCGTATTCTAAAAATAGTTCTTTAAATTATATAcc                                   >  2:742428/1‑141 (MQ=11)
                                                                                                          tAAAGCACGTATAATGATGATATTCAGCTAGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccac                                <  2:802396/141‑1 (MQ=255)
                                                                                                           aaaGCACTTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAATCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccacc                               <  2:644204/141‑1 (MQ=21)
                                                                                                           aaaGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTTAAAATAGTTCTTTAAATTATATACccacca                              >  2:825318/1‑141 (MQ=11)
                                                                                                            aaGCACGTATAATGATGATTTTCAGCTTGTACATATGAGATAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccacca                              >  1:158739/1‑141 (MQ=255)
                                                                                                            aaGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccacca                              <  1:637987/141‑1 (MQ=38)
                                                                                                            aaGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATGTTAAAAATAGTTCTTTAAATTATATACccacca                              >  2:636617/1‑141 (MQ=38)
                                                                                                             aGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTTAAAATAGTTCTTTAAATTATATACCCACcaca                            <  1:544077/141‑1 (MQ=11)
                                                                                                              gCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACcaca                            >  2:892724/1‑141 (MQ=38)
                                                                                                               cACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccaccaaat                           <  2:249910/141‑4 (MQ=38)
                                                                                                                aCGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTTAAAATAGTTCTTTAAATTATATACCCACCACAttt                         <  1:419402/141‑1 (MQ=11)
                                                                                                                 cGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACAttt                         >  2:940025/1‑141 (MQ=38)
                                                                                                                     taATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGTACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTtggtg                     >  1:56717/1‑141 (MQ=38)
                                                                                                                       atgatgATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGga                   >  2:809215/1‑141 (MQ=38)
                                                                                                                       ataatgatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCAAATGtggtggg                   <  2:435789/138‑2 (MQ=255)
                                                                                                                            gatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAAcc              <  1:478981/141‑1 (MQ=38)
                                                                                                                            gatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAAcc              >  2:62465/1‑141 (MQ=38)
                                                                                                                            gatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAAcc              <  1:635745/141‑1 (MQ=37)
                                                                                                                             atTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCt             <  1:741407/141‑1 (MQ=38)
                                                                                                                             atTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCt             >  2:281514/1‑141 (MQ=38)
                                                                                                                               tttCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTaa           >  2:594578/1‑141 (MQ=40)
                                                                                                                                  cAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTaaaaa        >  1:896229/1‑141 (MQ=40)
                                                                                                                                     cTTGTACAAAGGAGACAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATCGTTCTCTAAATTATATACCAACCACCTTTGGTGGAGAACCTaaaaaaaa     >  2:553972/1‑141 (MQ=14)
                                                                                                                                     cTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAATGATCTAAAAGTTGTATTTTAAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGAaaaaaaaaaaaaaa      <  2:1049696/141‑1 (MQ=11)
                                                                                                                                      ttGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAg    >  2:1071178/1‑141 (MQ=255)
                                                                                                                                        gTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCa  <  1:825648/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                             
ATACAATTTATTACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCA  >  CP000730/2291941‑2292217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: