BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,292,140	G→A	5.8%	intergenic (+126/‑10)	USA300HOU_2155 → / → USA300HOU_2156	transposase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,292,140	0	G	A	5.8%	373.9 / 7.8	121	intergenic (+126/‑10)	USA300HOU_2155/USA300HOU_2156	transposase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (61/52); new base A (4/3); total (65/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 124 total aligned reads displayed.

AAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCCTCTGAT  >  CP000730/2292000‑2292280

aaaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATGATAAAAg                                                                                                                                              <  1:858881/141‑1 (MQ=38)

aaaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAg                                                                                                                                              <  1:1023405/141‑1 (MQ=38)

 aaTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGc                                                                                                                                             <  1:602292/141‑1 (MQ=39)

  atttaccAAGTGAATTTCTTTTGGTTACCCACATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGCGAAAAACAGAAGCCACCCCACCCCAATAATGGACTGGCCGCCTAATAATAAAAGct                                                                                                                                            <  2:270318/135‑1 (MQ=11)

  aTTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGct                                                                                                                                            <  2:21701/141‑1 (MQ=40)

     aaTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTa                                                                                                                                         <  2:557344/141‑1 (MQ=40)

     aaTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACCTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTa                                                                                                                                         <  1:301327/141‑1 (MQ=255)

      aTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTaa                                                                                                                                        <  2:1070811/141‑1 (MQ=40)

      aTCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTaa                                                                                                                                        >  1:111452/1‑141 (MQ=40)

        cAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTaaaa                                                                                                                                      >  2:68875/1‑141 (MQ=40)

           gTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGtt                                                                                                                                   >  1:870118/1‑141 (MQ=39)

            tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                                                                                  <  1:458787/141‑1 (MQ=38)

            tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                                                                                  <  1:1056287/141‑1 (MQ=38)

            tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                                                                                  <  1:554746/141‑1 (MQ=38)

            tGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTg                                                                                                                                  >  1:35081/1‑141 (MQ=38)

            cgAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTg                                                                                                                                  >  1:212360/2‑141 (MQ=2)

             gAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGt                                                                                                                                 <  1:13767/141‑1 (MQ=38)

               aTTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTAt                                                                                                                               <  2:42462/141‑1 (MQ=38)

                tttCTCTTGGTTACAGAAATTTCAACAAATTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAACGCTCTCAAAGTTGTAtt                                                                                                                              >  2:247740/1‑141 (MQ=255)

                  tctcTTGGTTAGAGAAATTGCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGCTGTAtttt                                                                                                                            >  2:496492/1‑141 (MQ=255)

                   cTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTa                                                                                                                           >  1:686434/1‑141 (MQ=38)

                    ttttGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaa                                                                                                                          >  1:493358/1‑141 (MQ=37)

                    ttttGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaa                                                                                                                          >  1:192292/1‑141 (MQ=38)

                     tgtGGTTACAGAAATTTCAACAACTATAAAGCACGTATAATGATGATGTTCAGCATGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaaa                                                                                                                         <  2:756584/139‑1 (MQ=255)

                      ttGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTaaaa                                                                                                                        >  1:866925/1‑141 (MQ=38)

                          ttACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAg                                                                                                                    <  2:473054/141‑1 (MQ=38)

                           tACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGt                                                                                                                   <  1:393222/141‑1 (MQ=38)

                            aCAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGtt                                                                                                                  >  1:1042477/1‑141 (MQ=38)

                                aaaTTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCttt                                                                                                              >  2:96611/1‑141 (MQ=38)

                                aaaTTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCttt                                                                                                              >  1:249910/1‑141 (MQ=38)

                                    ttCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAAt                                                                                                          <  1:495051/141‑1 (MQ=38)

                                     tCAACAACTGTAAAGCACGTATAATGATGATTGTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAAtt                                                                                                         <  2:216970/141‑1 (MQ=255)

                                       aacaacTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATtat                                                                                                       >  2:748289/1‑141 (MQ=38)

                                       aacaacTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATtat                                                                                                       >  1:315480/1‑141 (MQ=38)

                                           acTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAATAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCACTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATAc                                                                                                   >  2:795363/1‑141 (MQ=255)

                                            cTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGCGAAAAAAAGAAGACAACCACGCCCAATAATGGACTGGCAGCCTAATAATAAAAGCACTAAAAGTCGTATTCTAAAAATAGTTCTTTAAATTATATAcc                                                                                                  >  2:742428/1‑141 (MQ=11)

                                               tAAAGCACGTATAATGATGATATTCAGCTAGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccac                                                                                               <  2:802396/141‑1 (MQ=255)

                                                aaaGCACTTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAATCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccacc                                                                                              <  2:644204/141‑1 (MQ=21)

                                                aaaGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTT‑AAAATAGTTCTTTAAATTATATACccacca                                                                                             >  2:825318/1‑141 (MQ=11)

                                                 aaGCACGTATAATGATGATTTTCAGCTTGTACATATGAGATAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccacca                                                                                             >  1:158739/1‑141 (MQ=255)

                                                 aaGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccacca                                                                                             <  1:637987/141‑1 (MQ=38)

                                                 aaGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATGTTAAAAATAGTTCTTTAAATTATATACccacca                                                                                             >  2:636617/1‑141 (MQ=38)

                                                  aGCACGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTT‑AAAATAGTTCTTTAAATTATATACCCACcaca                                                                                           <  1:544077/141‑1 (MQ=11)

                                                   gCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACcaca                                                                                           >  2:892724/1‑141 (MQ=38)

                                                    cACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccaccaaat                                                                                          <  2:249910/141‑4 (MQ=38)

                                                     aCGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACccaccaaatg                                                                                         <  1:793069/141‑5 (MQ=38)

                                                     aCGTATAATGATGATTTTCAGCTTATACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTT‑AAAATAGTTCTTTAAATTATATACCCACCACAttt                                                                                        <  1:419402/141‑1 (MQ=11)

                                                      cGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACAttt                                                                                        >  2:940025/1‑141 (MQ=38)

                                                          taATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGTACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTtggtg                                                                                    >  1:56717/1‑141 (MQ=38)

                                                            ataatgatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCAAATGtggtggg                                                                                  <  2:435789/138‑2 (MQ=255)

                                                                 gatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAAcc                                                                             <  1:478981/141‑1 (MQ=38)

                                                                 gatTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAAcc                                                                             >  2:62465/1‑141 (MQ=38)

                                                                  atTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCt                                                                            >  2:281514/1‑141 (MQ=38)

                                                                  atTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCt                                                                            <  1:741407/141‑1 (MQ=38)

                                                                    tttCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTaa                                                                          >  2:594578/1‑141 (MQ=40)

                                                                       cAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTaaaaa                                                                       >  1:896229/1‑141 (MQ=40)

                                                                          cTTGTACAAAGGAGACAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATCGTTCTCTAAATTATATACCAACCACCTTTGGTGGAGAACCTaaaaaaaa                                                                    >  2:553972/1‑141 (MQ=14)

                                                                           ttGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAg                                                                   >  2:1071178/1‑141 (MQ=255)

                                                                             gTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCa                                                                 <  1:825648/141‑1 (MQ=255)

                                                                               aCAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACt                                                               <  2:690739/141‑1 (MQ=255)

                                                                                cAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACtt                                                              <  1:306359/141‑1 (MQ=255)

                                                                                   aGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTccc                                                           <  2:505564/141‑1 (MQ=255)

                                                                                    ggAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCa                                                          <  2:596515/141‑1 (MQ=255)

                                                                                     gagaAAAAAAGAAGACAACCATGCCCATTATTGGACTGGCCGCCTAATAATAAAAGCTCTAAGAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCaa                                                         <  2:726068/141‑1 (MQ=255)

                                                                                     gagaAAAAAAGAAGACAAACAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGAAAAAACTAAAAAAAAGCACTTCCCaa                                                         <  2:841014/141‑1 (MQ=255)

                                                                                     gaaaaaaaaaGAAGACAAACAGGCCAAATAGAGTACTGGCAGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTAAGTGGAGAACCTAAAAAAAAGCACTTCCCaa                                                         <  2:397960/138‑1 (MQ=17)

                                                                                       gaAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAACTCTAAAAGTTGTAATTT‑AAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCaaaaa                                                      >  1:757313/1‑141 (MQ=255)

                                                                                        aaaaaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCaaaaa                                                      <  1:568106/141‑1 (MQ=255)

                                                                                        aaaaaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCaaaaa                                                      <  1:330944/141‑1 (MQ=255)

                                                                                          aaaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATg                                                    >  2:485452/1‑141 (MQ=255)

                                                                                          aaaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATg                                                    >  1:83784/1‑141 (MQ=255)

                                                                                          aaaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATg                                                    >  1:685559/1‑141 (MQ=255)

                                                                                          aaaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATg                                                    >  1:820926/1‑141 (MQ=255)

                                                                                           aaaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATgg                                                   <  1:759556/141‑1 (MQ=255)

                                                                                            aaaGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGa                                                  <  1:1062537/141‑1 (MQ=255)

                                                                                                   aCAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCa                                           >  1:407491/1‑141 (MQ=255)

                                                                                                      aCCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAagt                                        <  2:345215/141‑1 (MQ=255)

                                                                                                        cAAGCCCAATAATGGACTGGCCGCCTAATAATGAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAagtag                                      >  2:322971/1‑141 (MQ=255)

                                                                                                          aGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTg                                    >  2:851324/1‑141 (MQ=255)

                                                                                                          aGCCAAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACAACATTTGGTGGAGAACCTAAAAAAAAAAAAAAAAAAAAAATGGAAAGTGCAAGTAGTg                                    <  2:27592/141‑1 (MQ=14)

                                                                                                           gCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGa                                   >  1:987590/1‑141 (MQ=255)

                                                                                                                ataatGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCAt                              <  1:138654/141‑1 (MQ=255)

                                                                                                                 gaatTGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGTTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATa                             <  2:662479/140‑1 (MQ=255)

                                                                                                                        cTGGCCGCCTAATAATAAAAGCTCTAAAAGGTGTATTGTAAGAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGagt                      >  2:103594/1‑139 (MQ=255)

                                                                                                                             cgccTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAAc                 >  2:231110/1‑141 (MQ=255)

                                                                                                                              gccTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAAcc                >  1:440039/1‑141 (MQ=255)

                                                                                                                               ccTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACct               <  1:735314/141‑1 (MQ=255)

                                                                                                                                 taatcataaAAGCTCTAAAAGTCGTATTTTAAAAAGAGTTCTTTAAATTATATCCCCACCACATTTGGTGAAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACctct             <  2:803529/141‑1 (MQ=255)

                                                                                                                                  aataataaAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTg            >  2:820097/1‑141 (MQ=255)

                                                                                                                                  aataataaAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTg            <  2:739304/141‑1 (MQ=255)

                                                                                                                                   ataataaAAGCTCTAAGAGTTGTATTGTAAAAATAGTTCTTTAAATTATATACCCACCACAGTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGa           <  2:617325/141‑1 (MQ=255)

                                                                                                                                      ataaAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGc                                 >  1:711292/1‑116 (MQ=255)

                                                                                                                                      ataaAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGc                                 <  2:711292/116‑1 (MQ=255)

                                                                                                                                       taaAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCct       >  2:797349/1‑141 (MQ=255)

                                                                                                                                       taaAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCct       >  2:942195/1‑141 (MQ=255)

                                                                                                                                        aaaaGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCctc      <  1:862363/141‑1 (MQ=255)

                                                                                                                                          aaGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCCTCTg    >  2:225083/1‑141 (MQ=255)

                                                                                                                                           aGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCCTCTGa   <  1:154461/141‑1 (MQ=255)

                                                                                                                                           aGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCCTCTGa   >  1:985526/1‑141 (MQ=255)

                                                                                                                                            gCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCCTCTGAt  >  2:109003/1‑141 (MQ=255)

AAATTAATCAAGTGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTCAGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAATAATAAAAGCTCTAAAAGTTGTATTTTAAAAATAGTTCTTTAAATTATATACCCACCACATTTGGTGGAGAACCTAAAAAAAAGCACTTCCCAAAAATGGAAAGTGCAAGTAGTGAGCCATAGAGGATTCGAACCTCTGACCCTCTGAT  >  CP000730/2292000‑2292280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`