Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,338,816 A→G 5.2% K194R (AAA→AGA)  hysA → hyaluronate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,338,8160AG 5.2% 349.7 / 5.9 114K194R (AAA→AGA) hysAhyaluronate lyase
Reads supporting (aligned to +/- strand):  ref base A (55/53);  new base G (4/2);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 116 total aligned reads displayed.

GATGCCCTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTA  >  CP000730/2338677‑2338953
                                                                                                                                           |                                                                                                                                         
gATGCCCTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTaaa                                                                                                                                          >  2:1079135/1‑141 (MQ=255)
    cccTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAACGAATCAATTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCAt                                                                                                                                      <  1:19125/141‑1 (MQ=255)
         gAGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACa                                                                                                                                 >  1:597006/1‑141 (MQ=255)
          aGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACaa                                                                                                                                >  2:85753/1‑141 (MQ=255)
          aGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACaa                                                                                                                                <  2:818357/141‑1 (MQ=255)
           gTGGCTGCATAAAAATGCATGTGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACaaa                                                                                                                               >  1:184405/1‑141 (MQ=255)
           gTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACaaa                                                                                                                               >  1:67989/1‑141 (MQ=255)
             ggCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATa                                                                                                                             >  1:994141/1‑141 (MQ=255)
              gCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATAc                                                                                                                            <  2:1028631/141‑1 (MQ=255)
                tGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGc                                                                                                                          <  1:80928/141‑1 (MQ=255)
                 gCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCt                                                                                                                         <  2:84146/141‑1 (MQ=255)
                         aTGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGc                                                                                                                 >  1:577004/1‑141 (MQ=255)
                         aTGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGc                                                                                                                 >  1:77169/1‑141 (MQ=255)
                           gCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTg                                                                                                               <  2:411005/141‑1 (MQ=255)
                              tatGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAAt                                                                                                            >  1:631718/1‑141 (MQ=255)
                              tatGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAAt                                                                                                            <  2:22095/141‑1 (MQ=255)
                              tatGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAAt                                                                                                            >  1:980770/1‑141 (MQ=255)
                                tGGAAAAAAAACAAATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGa                                                                                                          <  2:888162/141‑1 (MQ=255)
                                 ggAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGAt                                                                                                         >  1:856908/1‑141 (MQ=255)
                                      aGAATCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAAtt                                                                                                    >  1:1057734/1‑141 (MQ=255)
                                      aGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAAtt                                                                                                    <  2:273062/141‑1 (MQ=255)
                                      aGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAAtt                                                                                                    <  1:564690/141‑1 (MQ=255)
                                      aGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAAtt                                                                                                    >  2:956705/1‑141 (MQ=255)
                                       gAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAAttt                                                                                                   <  1:325264/141‑1 (MQ=255)
                                       gAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAAttt                                                                                                   <  1:255728/141‑1 (MQ=255)
                                         aCCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTc                                                                                                 >  2:990491/1‑141 (MQ=255)
                                         aCCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTc                                                                                                 >  2:317935/1‑141 (MQ=255)
                                         aCCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTc                                                                                                 >  1:316945/1‑141 (MQ=255)
                                          ccAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCa                                                                                                >  2:923247/1‑141 (MQ=255)
                                          ccAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCa                                                                                                >  1:131822/1‑141 (MQ=255)
                                            aGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCaaa                                                                                              <  1:260515/141‑1 (MQ=255)
                                             gATAAAACAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAt                                                                                             <  2:810606/141‑1 (MQ=255)
                                              aTAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATg                                                                                            <  1:962020/141‑1 (MQ=255)
                                                aaaaaaGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATgaa                                                                                          <  1:365023/141‑1 (MQ=255)
                                                  aaaaGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATgaaga                                                                                        >  1:1003113/1‑141 (MQ=255)
                                                   aaaGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATgaagaa                                                                                       >  1:740981/1‑141 (MQ=255)
                                                    aaGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGaagaaa                                                                                      >  1:1059126/1‑141 (MQ=255)
                                                      gTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAGCTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAAGTATCAATTTTCAAATTaagaaaat                                                                                    >  2:270289/1‑140 (MQ=255)
                                                      gTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGaagaaaag                                                                                    >  1:80639/1‑141 (MQ=255)
                                                      gTAAAAGAATTAAGGGAGAATTTGACTAAAACAACTGGCAAGAAGACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCGTATATTGCTGAATGATCAATTTTCAAAGGaagaaaag                                                                                    >  2:270276/1‑141 (MQ=255)
                                                       tAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGaagaaaaga                                                                                   <  1:1022014/141‑1 (MQ=255)
                                                         aaaGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAagaaaagaaa                                                                                 <  1:513251/141‑1 (MQ=255)
                                                         aaaGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAagaaaagaaa                                                                                 >  1:137440/1‑141 (MQ=255)
                                                          aaGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAgaaaagaaaa                                                                                >  2:752203/1‑141 (MQ=255)
                                                          aaGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAgaaaagaaaa                                                                                <  1:452788/141‑1 (MQ=255)
                                                                 aaGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCAc                                                                         >  2:1023171/1‑141 (MQ=255)
                                                                  aGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACt                                                                        <  1:652112/141‑1 (MQ=255)
                                                                   gTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTg                                                                       >  1:581438/1‑141 (MQ=255)
                                                                     gagaATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGcc                                                                     >  2:845553/1‑141 (MQ=255)
                                                                        aaTTTTACTGAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCt                                                                  >  1:954776/1‑141 (MQ=255)
                                                                             tACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTaa                                                             >  1:604825/1‑141 (MQ=255)
                                                                             tACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTaa                                                             <  2:429698/141‑1 (MQ=255)
                                                                               cTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTaaaa                                                           >  1:342694/1‑141 (MQ=255)
                                                                               cTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTaaaa                                                           >  2:287004/1‑141 (MQ=255)
                                                                               cTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGACCCTATTaaaa                                                           >  1:877618/1‑141 (MQ=255)
                                                                                tAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAAc                                                          <  2:641957/141‑1 (MQ=255)
                                                                                 aaaaCAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACt                                                         <  1:769648/141‑1 (MQ=255)
                                                                                 aaaaCAACTGGCAAGAATACCAACTAAAATCGGTGGGATTATGAAATGGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAGAAAAAAAAAAATTCACTGCCCCTATTAAAACt                                                         <  2:77169/141‑1 (MQ=255)
                                                                                   aacaacTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACttt                                                       >  1:362304/1‑141 (MQ=255)
                                                                                   aacaacTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACttt                                                       >  2:231540/1‑141 (MQ=255)
                                                                                     caacTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCg                                                     <  1:956705/141‑1 (MQ=255)
                                                                                     caacTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCg                                                     <  2:930412/141‑1 (MQ=255)
                                                                                      aacTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGc                                                    <  2:1006737/141‑1 (MQ=255)
                                                                                       acTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGcc                                                   <  2:1020120/141‑1 (MQ=255)
                                                                                        cTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGccc                                                  <  1:806819/141‑1 (MQ=255)
                                                                                        cTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGccc                                                  <  2:253794/141‑1 (MQ=255)
                                                                                         tGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGcccc                                                 >  2:1014252/1‑141 (MQ=255)
                                                                                          ggCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCa                                                <  1:345550/141‑1 (MQ=255)
                                                                                          ggCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCa                                                >  1:985436/1‑141 (MQ=255)
                                                                                          ggCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCa                                                <  1:795519/141‑1 (MQ=255)
                                                                                          ggCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCa                                                >  1:985502/1‑141 (MQ=255)
                                                                                           gCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAg                                               >  1:890996/1‑141 (MQ=255)
                                                                                           gCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAg                                               >  2:144514/1‑141 (MQ=255)
                                                                                            cAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATCGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGa                                              >  2:321351/1‑141 (MQ=255)
                                                                                               gAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAg                                           <  1:881713/141‑1 (MQ=255)
                                                                                               gAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAg                                           >  2:456540/1‑141 (MQ=255)
                                                                                                  tACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGa                                        <  1:822862/141‑1 (MQ=255)
                                                                                                     agctttAAATTGGTGGGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGatga                                     >  1:888332/5‑138 (MQ=255)
                                                                                                          tAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAatat                                <  1:1079072/141‑1 (MQ=255)
                                                                                                            aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                              <  2:1034856/141‑1 (MQ=255)
                                                                                                            aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                              <  1:406517/141‑1 (MQ=255)
                                                                                                            aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                              >  2:720292/1‑141 (MQ=255)
                                                                                                             aTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtattat                             <  1:522008/141‑1 (MQ=255)
                                                                                                               tggtggGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTAtct                           >  2:438495/1‑141 (MQ=255)
                                                                                                                 gtggGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTAtcttc                         >  1:751044/1‑141 (MQ=255)
                                                                                                                  tggGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTAtcttct                        <  1:101800/141‑1 (MQ=255)
                                                                                                                   gggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTg                       <  2:716206/141‑1 (MQ=255)
                                                                                                                    ggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGt                      >  2:728237/1‑141 (MQ=255)
                                                                                                                         aTGAAATTGGAACACCTAGATCATTAACAAATACGCTTGTATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGTAGGaa                 >  2:376482/1‑141 (MQ=255)
                                                                                                                            aaaTTGGAACACCTAAATCATTAACAAATGCGCTTATATTGCTGAATGATGTAGGTTCAAATGAATAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg              <  1:539855/141‑1 (MQ=255)
                                                                                                                            aaaTTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg              <  1:527606/141‑1 (MQ=255)
                                                                                                                            aaaTTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg              <  2:581438/141‑1 (MQ=255)
                                                                                                                                 ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa         <  1:88404/141‑1 (MQ=255)
                                                                                                                                 ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa         >  1:1076303/1‑141 (MQ=255)
                                                                                                                                 ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAAAAAAAAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa         <  2:631718/141‑1 (MQ=255)
                                                                                                                                  gAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAAc        >  2:228895/1‑141 (MQ=255)
                                                                                                                                     cacCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTg     >  2:281965/1‑141 (MQ=255)
                                                                                                                                      acCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGc    <  1:710183/141‑1 (MQ=255)
                                                                                                                                      acCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAAAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGc    <  2:160487/141‑1 (MQ=255)
                                                                                                                                        cTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTa  <  1:752203/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GATGCCCTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCAGATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTA  >  CP000730/2338677‑2338953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: