Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,398,692 T→C 5.3% I188I (ATT→ATC fdhD → formate dehydrogenase accessory protein FdhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,398,6920TC 5.3% 277.8 / 2.2 93I188I (ATT→ATCfdhDformate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  ref base T (48/40);  new base C (3/2);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GATTACAAAGTGCAAGTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCTTAGC  >  CP000730/2398553‑2398832
                                                                                                                                           |                                                                                                                                            
gATTACAAAGTGCAAGTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTc                                                                                                                                             >  1:130293/1‑141 (MQ=255)
       aaGTGCAAGTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTCTTCAGCGACa                                                                                                                                      >  1:110911/1‑141 (MQ=255)
             aaGTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATCAGCGCTGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAACTTATATGGATTTTGTATTCAGCGACATATTAc                                                                                                                                <  2:557610/141‑1 (MQ=255)
              aGTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACt                                                                                                                               <  2:898308/141‑1 (MQ=255)
               gTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTTTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTg                                                                                                                              >  2:310204/1‑141 (MQ=255)
               gTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTg                                                                                                                              >  1:390383/1‑141 (MQ=255)
               gTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATCCAGCGACATATTACTg                                                                                                                              >  2:375073/1‑141 (MQ=255)
                 cATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTc                                                                                                                            >  1:877251/1‑141 (MQ=255)
                 cATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTc                                                                                                                            <  1:408118/141‑1 (MQ=255)
                 cATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTc                                                                                                                            <  1:672560/141‑1 (MQ=255)
                 cATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTc                                                                                                                            <  2:1039929/141‑1 (MQ=255)
                      ttACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGGGGGTTGGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCgaga                                                                                                                       >  1:792504/1‑141 (MQ=255)
                      ttACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCgaga                                                                                                                       >  1:729440/1‑141 (MQ=255)
                      ttACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGGATTCAGCGACATATTACTGTCCgaga                                                                                                                       >  2:860740/1‑141 (MQ=255)
                         ccAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATaa                                                                                                                    >  2:567193/1‑141 (MQ=255)
                         ccAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATaa                                                                                                                    >  1:214888/1‑141 (MQ=255)
                            ataaaCAGGGGGCTTTCATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATGTGTAGACATAACGCTCTATATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGt                                                                                                                 <  2:1001750/139‑1 (MQ=255)
                              aaaCAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTAt                                                                                                               <  2:132679/141‑1 (MQ=255)
                              aaaCAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTAt                                                                                                               <  1:523197/141‑1 (MQ=255)
                               aaCAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTAtt                                                                                                              <  2:19144/141‑1 (MQ=255)
                                   ggTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAAtt                                                                                                          >  2:821773/1‑141 (MQ=255)
                                   ggTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAAtt                                                                                                          >  1:768638/1‑141 (MQ=255)
                                   ggTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAAtt                                                                                                          >  2:12168/1‑141 (MQ=255)
                                   ggTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAAtt                                                                                                          <  1:970664/141‑1 (MQ=255)
                                    gTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAAttt                                                                                                         >  1:361886/1‑141 (MQ=255)
                                        cTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAg                                                                                                     >  1:379566/1‑141 (MQ=255)
                                         ttACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATaaa                                                                                                                   >  2:824925/1‑126 (MQ=255)
                                         ttACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATaaa                                                                                                                   <  1:824925/126‑1 (MQ=255)
                                          tACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTg                                                                                                   <  1:90164/141‑1 (MQ=255)
                                           aCATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATACATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTgg                                                                                                  >  2:532916/1‑141 (MQ=255)
                                           aCATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTgg                                                                                                  >  1:966967/1‑141 (MQ=255)
                                                  gctgctATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTAtt                                                                                           >  2:284981/1‑141 (MQ=255)
                                                  gctgctATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTAtt                                                                                           >  1:804919/1‑141 (MQ=255)
                                                  gctgctATAAGCGATGGCTGGACATTTTTTGTACATCGACAAGACATTGGTAGACATACCGCTCTAGATAAATTATACGGCTTTTGTATCCAGCGACATATTACTGTCAGAGATAAAGTATTAATGTTTAGTGGACGTAtt                                                                                           >  2:1045368/1‑141 (MQ=255)
                                                     gctATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTtca                                                                                        >  1:387022/1‑141 (MQ=255)
                                                     gctATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGAATGTAGATCCAGCGACAGATTACTGTCCGAGATAAAGGATTAATTTTTAGTGGACGTATTtca                                                                                        >  1:317961/1‑141 (MQ=255)
                                                      ctATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTtcat                                                                                       >  2:76117/1‑141 (MQ=255)
                                                      cgaaaaGCGATGGTTTCACATTTTTTGTAAATCGACAAAACATTGGTAGACATAACGCACTAGAAAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTATAGTGGACGTATTtcat                                                                                       <  2:566430/137‑1 (MQ=255)
                                                        ataAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTtcatca                                                                                     <  2:362642/141‑1 (MQ=255)
                                                         aaaGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAg                                                                                    <  1:1056189/140‑1 (MQ=255)
                                                          atgcantGGTTTGACATTTTTTGTACATAGACAAGACATTGGTAAACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGANATANAGTATTAATTTTTAGTGGACGTATTTCATCAGa                                                                                   <  1:326571/135‑1 (MQ=255)
                                                           aGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGaa                                                                                  <  1:1005567/141‑1 (MQ=255)
                                                           aGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGaa                                                                                  >  1:90998/1‑141 (MQ=255)
                                                           aGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGaa                                                                                  >  1:91116/1‑141 (MQ=255)
                                                              gATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAAtt                                                                               <  1:152054/141‑1 (MQ=255)
                                                               aTGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAAttt                                                                              <  1:533587/141‑1 (MQ=255)
                                                                  gTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTttaa                                                                           <  2:531154/141‑1 (MQ=255)
                                                                     tGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTttaatta                                                                        >  2:1025225/1‑141 (MQ=255)
                                                                          ttttttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGcc                                                                   >  2:275048/1‑141 (MQ=255)
                                                                           tttttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCATAAATTTTAATTAAAGCCg                                                                  <  1:527207/141‑1 (MQ=255)
                                                                           tttttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATCCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAgaca                                                                  <  2:358373/141‑4 (MQ=255)
                                                                            ttttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGc                                                                 <  2:247453/141‑1 (MQ=255)
                                                                            ttttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGc                                                                 <  2:758810/141‑1 (MQ=255)
                                                                            ttttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGc                                                                 <  2:1000297/141‑1 (MQ=255)
                                                                             tttGTACATCGACAAGACATTGTTAGACATAACTCTCTAAATAAATTATATAAATTTTGTATTCATCGACATGTTACTGTCCGAGATAAAGTATTAATTTTTAGTTGACGTATTTCATCAGAAATTttaaaaaaaaaaaaa                                                                <  2:295227/141‑12 (MQ=255)
                                                                             tttGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCa                                                                <  1:368028/141‑1 (MQ=255)
                                                                               tGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCaaa                                                              <  1:825177/141‑1 (MQ=255)
                                                                                gTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCaaaa                                                             <  1:808429/141‑1 (MQ=255)
                                                                                gTACATAGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGTAACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGGATTTCATCAGAAAATTTAATTAAAGACGAaaaa                                                             <  2:130293/141‑1 (MQ=255)
                                                                                 tACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCaaaaa                                                            <  1:745058/141‑1 (MQ=255)
                                                                                  aCATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAAt                                                           >  2:699120/1‑141 (MQ=255)
                                                                                   cATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAAtt                                                          >  1:695046/1‑141 (MQ=255)
                                                                                     tCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTgg                                                        >  1:1047399/1‑141 (MQ=255)
                                                                                        aCAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGgtgt                                                     <  2:835504/141‑1 (MQ=255)
                                                                                            gACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGc                                                 <  1:172630/141‑1 (MQ=255)
                                                                                             aCATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCg                                                <  1:624060/141‑1 (MQ=255)
                                                                                             aCATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTCCATCAGAAATTTTAATTAAAGACGCAAAGATTGGTGTTGGCg                                                >  2:946775/1‑141 (MQ=255)
                                                                                                ttGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCa                                             >  2:294302/1‑141 (MQ=255)
                                                                                                 tGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCAt                                            >  1:1067409/1‑141 (MQ=255)
                                                                                                  ggTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCAtt                                           >  1:255962/1‑141 (MQ=255)
                                                                                                   gTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCAttt                                          >  1:694639/1‑141 (MQ=255)
                                                                                                    aaGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAAAAAAAAAAAAAAAAATTGGTGTTGGCGTCAtttt                                         <  2:346883/140‑1 (MQ=255)
                                                                                                        cATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCt                                     >  1:664809/1‑141 (MQ=255)
                                                                                                        cATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCt                                     >  2:594920/1‑141 (MQ=255)
                                                                                                        cATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCt                                     >  1:392984/1‑141 (MQ=255)
                                                                                                          tAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAAGTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTaa                                   >  1:801779/1‑141 (MQ=255)
                                                                                                             cGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATc                                >  2:136565/1‑141 (MQ=255)
                                                                                                              gCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCCTGAGGAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCa                               >  1:884679/1‑141 (MQ=255)
                                                                                                                 ctAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCt                            <  2:1075942/141‑1 (MQ=255)
                                                                                                                    gATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAAAAAAAGAAAAAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCg                         <  2:85664/141‑1 (MQ=255)
                                                                                                                     aTAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGACGAAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGa                        <  2:829732/141‑1 (MQ=255)
                                                                                                                       aaaTTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACa                      >  1:1057948/1‑141 (MQ=255)
                                                                                                                        aaTTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACaa                     >  1:360674/1‑141 (MQ=255)
                                                                                                                            atatGGATTTTGTATCCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCGAAAATCGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCt                 <  2:874394/141‑1 (MQ=255)
                                                                                                                             tatGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCtt                >  2:686256/1‑141 (MQ=255)
                                                                                                                                ggATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCt             >  1:832133/1‑141 (MQ=255)
                                                                                                                                ggATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCt             >  1:615700/1‑141 (MQ=255)
                                                                                                                                  aTTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGt           <  2:324603/141‑1 (MQ=255)
                                                                                                                                   ttttGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTTTTTGTCGTTTTTCATCAGAAATTTTAATTAAAGCTGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTCTa          >  2:252304/1‑141 (MQ=255)
                                                                                                                                   ttttGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTa          >  1:521084/1‑141 (MQ=255)
                                                                                                                                       gTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCt      >  2:339010/1‑141 (MQ=255)
                                                                                                                                         aTTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAAATAAAGACGAAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCTTa    <  2:829779/141‑1 (MQ=255)
                                                                                                                                         aTTCAGCGACAACTAACCGTCCCAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATAAAAACCACAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCTTa    <  2:317745/141‑1 (MQ=255)
                                                                                                                                          ttCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCTTAg   >  2:880834/1‑141 (MQ=255)
                                                                                                                                           tCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCTTAGc  <  2:729440/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GATTACAAAGTGCAAGTCATATTTACCAAGAAACAGGTGGCTTACATAATGCTGCTATAAGCGATGGTTTGACATTTTTTGTACATCGACAAGACATTGGTAGACATAACGCTCTAGATAAATTATATGGATTTTGTATTCAGCGACATATTACTGTCCGAGATAAAGTATTAATTTTTAGTGGACGTATTTCATCAGAAATTTTAATTAAAGCCGCAAAAATTGGTGTTGGCGTCATTTTATCTAAATCAGCTCCGACAACGCTTGCTGTAACCTTAGC  >  CP000730/2398553‑2398832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: