Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,559,840 T→G 9.1% intergenic (+34/+89) bcr → / ← USA300HOU_2421 MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,559,8400TG 9.1% 239.5 / 13.6 88intergenic (+34/+89)bcr/USA300HOU_2421MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/43);  new base G (5/3);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

TTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTA  >  CP000730/2559702‑2559977
                                                                                                                                          |                                                                                                                                         
tttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatct                                                                                                                                         <  2:35980/141‑1 (MQ=255)
 ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                        >  1:31850/1‑141 (MQ=255)
 ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                        <  1:939555/141‑1 (MQ=255)
  tAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctat                                                                                                                                       <  2:244572/141‑1 (MQ=255)
   aaTACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctatc                                                                                                                                      >  2:339998/1‑141 (MQ=255)
      aCATCACCATATATGATTATTATCTTCATTACAGCCTTTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAATAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTATCAAAGATATCTATCttt                                                                                                                                   >  1:1014644/1‑141 (MQ=255)
       cATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTg                                                                                                                                  <  1:627455/141‑1 (MQ=255)
          cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                               >  2:933653/1‑141 (MQ=255)
          cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                               >  1:507135/1‑141 (MQ=255)
             cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTgctaggg                                                                                                                            <  1:43213/141‑7 (MQ=39)
             cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                            >  2:990098/1‑141 (MQ=255)
             cATATATGATTATTATCGTCATTACAGCCATTCTATTAGTCAGTCTAAAAATGATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                            <  2:821461/141‑1 (MQ=255)
               tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                          <  2:221980/141‑1 (MQ=255)
               tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                          <  1:889107/141‑1 (MQ=255)
                atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttc                                                                                                                         <  2:150905/141‑1 (MQ=38)
                atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttc                                                                                                                         <  1:882930/141‑1 (MQ=255)
                 tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATCTCTAACTTTGTCAGGGttct                                                                                                                        >  1:58897/1‑141 (MQ=255)
                 tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                        <  2:792960/141‑1 (MQ=255)
                 tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                        <  2:351839/141‑1 (MQ=255)
                  atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAATACGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                       <  1:293486/141‑1 (MQ=255)
                  atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                       >  2:710753/1‑141 (MQ=255)
                    gATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTtcttct                                                                                                                     <  2:966186/141‑1 (MQ=255)
                             cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                            >  2:232756/1‑141 (MQ=255)
                             cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                            >  1:1049215/1‑141 (MQ=255)
                               tCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTAt                                                                                                          >  2:754954/1‑141 (MQ=255)
                                   tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga                                                                                                      <  2:494361/141‑1 (MQ=255)
                                        ccATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGaa                                                                                                 >  1:706415/1‑141 (MQ=255)
                                           ttCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCt                                                                                              >  1:194481/1‑141 (MQ=255)
                                              tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                           <  1:164267/141‑1 (MQ=255)
                                              tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                           >  2:944259/1‑141 (MQ=255)
                                               aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                          <  2:435504/141‑1 (MQ=255)
                                               aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                          >  1:155902/1‑141 (MQ=255)
                                                ttAGTCCGTCTACAAAACATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                         <  2:130118/141‑1 (MQ=255)
                                                ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                         <  1:1057831/141‑1 (MQ=255)
                                                   gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                      >  2:532994/1‑141 (MQ=255)
                                                   gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                      >  1:535117/1‑141 (MQ=255)
                                                    tcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaaa                                                                                     >  1:714611/1‑141 (MQ=255)
                                                       gtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAAtt                                                                                  <  1:685775/141‑1 (MQ=255)
                                                        tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                 <  1:390632/141‑1 (MQ=255)
                                                        tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                 >  2:223839/1‑141 (MQ=255)
                                                           aCAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGc                                                                              <  1:245040/141‑1 (MQ=255)
                                                            cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                             >  2:838504/1‑141 (MQ=255)
                                                            cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                             >  2:881869/1‑141 (MQ=255)
                                                             aaaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCt                                                                            >  1:543814/1‑141 (MQ=255)
                                                              aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                           <  2:836358/141‑1 (MQ=255)
                                                              aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                           <  1:419774/141‑1 (MQ=255)
                                                              aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                           <  2:175299/141‑1 (MQ=255)
                                                               aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTc                                                                          <  2:108461/141‑1 (MQ=255)
                                                               aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTc                                                                          <  1:295063/141‑1 (MQ=255)
                                                                 cattttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCAt                                                                        >  1:734045/12‑141 (MQ=255)
                                                                  attttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATa                                                                       >  1:703316/11‑141 (MQ=255)
                                                                    ttACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGc                                                                     >  2:565000/1‑141 (MQ=255)
                                                                        tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg                                                                 <  1:842232/141‑1 (MQ=255)
                                                                        tatAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAAATAGAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAACGAATCTTCAACTAAAATTACGCCTACAAAACaaag                                                                 <  2:23727/139‑3 (MQ=255)
                                                                           aaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACa                                                              >  1:789052/1‑141 (MQ=255)
                                                                            aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                             >  1:463095/1‑141 (MQ=255)
                                                                            aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                             <  2:716485/141‑1 (MQ=255)
                                                                            aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGACATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                             <  1:1063207/141‑1 (MQ=255)
                                                                             aaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCATCCAAAATTACGCCTTCATAGCAAGGACAtt                                                            <  2:321431/141‑1 (MQ=255)
                                                                                    aaaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTc                                                     <  1:452345/141‑1 (MQ=255)
                                                                                     aaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCa                                                    >  2:1003600/1‑141 (MQ=255)
                                                                                     aaaaGCAACATGTCGCATAACACTTCAACATAACTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTGCTTCTTTATGAATTAAGAGAGCGAATCTTCAACTAAAATTACGCCTTCATGGTGAGGACATTTCTATTCa                                                    <  2:965093/141‑1 (MQ=255)
                                                                                     aaaaACAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGACTTCATAGCAAGGACATTTCTATTCa                                                    <  2:652164/141‑1 (MQ=255)
                                                                                           aaCATGTCGCATAACACTTCAACATAATTAGTACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCTAGGACATTTCTATTCAATCAcg                                              <  2:1083175/141‑2 (MQ=255)
                                                                                            aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                             >  1:305915/1‑141 (MQ=255)
                                                                                            aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                             >  1:729393/1‑141 (MQ=255)
                                                                                            aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                             >  2:976977/1‑141 (MQ=255)
                                                                                                    cATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAg                                     <  2:1010271/141‑1 (MQ=255)
                                                                                                        acacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTCATATCAAGTACATTTCTATTCAATCACCCTTTAACAGGCAt                                 >  1:924064/1‑141 (MQ=255)
                                                                                                         cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                                <  1:628843/141‑1 (MQ=255)
                                                                                                         cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                                <  2:585800/141‑1 (MQ=255)
                                                                                                          acTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATcc                               <  1:607460/141‑1 (MQ=255)
                                                                                                            ttCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaa                             >  1:320962/1‑141 (MQ=255)
                                                                                                             tCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaaa                            <  1:55108/141‑1 (MQ=255)
                                                                                                                cataaagaaGAACCCTGACAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAAttt                         >  1:484418/9‑141 (MQ=37)
                                                                                                                  taaagaaGAACCCTGACAAAGATAGATATCTTTGCTAGGGTTC‑TAATTATGAATTATGAGGTCGCATCTTCAACTAAAATTACGCCTTGATAGCAAGGACATATCTATTCAAGCACCCTTTAACAGGCATCCAAACTTCTg                      >  2:801674/7‑141 (MQ=16)
                                                                                                                    aaTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGt                     <  2:285802/141‑1 (MQ=255)
                                                                                                                     aTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTACTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCAACCAAATTTCtata                    >  2:723263/1‑141 (MQ=255)
                                                                                                                      ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa                   >  2:710280/1‑141 (MQ=255)
                                                                                                                        ggAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACGAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata                 <  2:656745/140‑1 (MQ=255)
                                                                                                                        aGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata                 <  1:657919/141‑1 (MQ=255)
                                                                                                                           aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat              >  1:631342/1‑141 (MQ=255)
                                                                                                                           aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat              <  2:466930/141‑1 (MQ=255)
                                                                                                                            cccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtt             <  1:982570/141‑1 (MQ=255)
                                                                                                                             cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatat                >  1:765276/6‑137 (MQ=38)
                                                                                                                             cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatat                <  2:765276/132‑1 (MQ=38)
                                                                                                                                 gCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCa        >  2:768959/1‑141 (MQ=255)
                                                                                                                                  cAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCAc       >  2:618831/1‑141 (MQ=255)
                                                                                                                                  cAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCAc       >  1:56543/1‑141 (MQ=255)
                                                                                                                                   aaaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACt      >  1:453501/1‑141 (MQ=255)
                                                                                                                                    aaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACtt     <  2:1049215/141‑1 (MQ=255)
                                                                                                                                     aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACttt    >  2:228279/1‑140 (MQ=255)
                                                                                                                                      gATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgt   <  2:162065/141‑1 (MQ=255)
                                                                                                                                       atatCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgta  >  2:324544/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTA  >  CP000730/2559702‑2559977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑