Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,614,095	T→C	5.2%	L192L (TTA→TTG)	USA300HOU_2478 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,614,095	0	T	C	5.2%	295.4 / 5.1	95	L192L (TTA→TTG)	USA300HOU_2478	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (54/36);  new base C (2/3);  total (56/39)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

AAATGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGC  >  CP000730/2613957‑2614235                                                                                                                                           |                                                                                                                                             aaaTGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTaa                                                                                                                                            >  1:92546/1‑141 (MQ=255) aaaTGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTAGTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCGCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTaa                                                                                                                                            >  1:648860/1‑141 (MQ=255)  aaTGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATTATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAAt                                                                                                                                           >  1:860307/1‑141 (MQ=255)  aaTGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAAt                                                                                                                                           >  1:152067/1‑141 (MQ=255)  aaTGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAAt                                                                                                                                           >  1:298357/1‑141 (MQ=255)  aaTGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTGCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAAt                                                                                                                                           >  1:860585/1‑141 (MQ=255)     gTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCCTCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGa                                                                                                                                        <  2:914447/141‑1 (MQ=255)         aCTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACt                                                                                                                                    <  2:610952/141‑1 (MQ=255)             tagattttttgacccTACAGATGAGCCGTTTAAATCACCATCACCTTTTATTAATAATTTCGGAGCTTTGTTGGTTGGAATATTATATCTTTTTCGAAGTTGCTTCACATTGTAGTCATCGTTTTTCAATTGATATTTTGc                                                                                                                                <  2:901543/128‑1 (MQ=255)             tAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGc                                                                                                                                <  2:64079/141‑1 (MQ=255)              aaaTTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCt                                                                                                                               <  1:974359/141‑1 (MQ=255)              aaaTTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCt                                                                                                                               <  2:872945/141‑1 (MQ=255)                    ttGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATaa                                                                                                                         >  2:911426/1‑141 (MQ=255)                     tgtgTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAAc                                                                                                                        <  1:709886/141‑1 (MQ=255)                         tCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTgg                                                                                                                    <  2:968325/141‑1 (MQ=255)                                gatgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTc                                                                                                             <  1:843430/141‑1 (MQ=255)                                  tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                           >  1:969394/1‑141 (MQ=255)                                  tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                           >  2:1018226/1‑141 (MQ=255)                                  tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                           >  1:969398/1‑141 (MQ=255)                                     tCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGAtt                                                                                                        >  1:414551/1‑141 (MQ=255)                                     tCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGAtt                                                                                                        >  2:719570/1‑141 (MQ=255)                                      ccTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATta                                                                                                       >  2:23878/1‑141 (MQ=255)                                         tttAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTatat                                                                                                    >  1:1082763/1‑141 (MQ=255)                                          ttAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATg                                                                                                   >  1:534818/1‑141 (MQ=255)                                           tAAATCACCATCGCCTTTTATTAATAATTTATGTGCTTTCTTAGTTTGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGa                                                                                                  <  2:1050642/141‑1 (MQ=255)                                            aaaTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGaa                                                                                                 >  2:984254/1‑141 (MQ=255)                                              aTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAAt                                                                                               >  2:416811/1‑141 (MQ=255)                                               tCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATg                                                                                              >  2:199428/1‑141 (MQ=255)                                                 aCCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGTACATTCGGATTATATGAAATGTc                                                                                            >  1:54353/1‑141 (MQ=255)                                                  ccATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCa                                                                                           >  1:720590/1‑141 (MQ=255)                                                     tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                        >  2:329677/1‑141 (MQ=255)                                                     tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                        <  1:394550/141‑1 (MQ=255)                                                       gCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATc                                                                                      <  2:1035806/141‑1 (MQ=255)                                                        ccTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCt                                                                                     >  1:1017792/1‑141 (MQ=255)                                                         cTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCtt                                                                                    <  1:1023358/141‑1 (MQ=255)                                                          ttttATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCttt                                                                                   <  1:567498/141‑1 (MQ=255)                                                              attaATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATaa                                                                               >  2:334074/1‑141 (MQ=255)                                                                 aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                            <  1:418361/141‑1 (MQ=255)                                                                 aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                            <  2:351777/141‑1 (MQ=255)                                                                 aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                            <  2:846648/141‑1 (MQ=255)                                                                  ataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCAt                                                                           <  2:862139/141‑1 (MQ=255)                                                                    aatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTa                                                                         <  1:479228/141‑1 (MQ=255)                                                                     atTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTaa                                                                        >  2:244782/1‑141 (MQ=255)                                                                       ttAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAata                                                                      >  1:136658/1‑141 (MQ=255)                                                                       ttAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAata                                                                      >  1:95091/1‑141 (MQ=255)                                                                          ggTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCt                                                                   >  2:854498/1‑141 (MQ=255)                                                                           gTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCtt                                                                  >  1:306952/1‑141 (MQ=255)                                                                           gTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCtt                                                                  >  2:1043921/1‑141 (MQ=255)                                                                            agCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCttt                                                                 <  2:402280/140‑1 (MQ=21)                                                                              cTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTaa                                                               <  2:376506/141‑1 (MQ=255)                                                                                 tCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTaaatt                                                            <  1:603127/141‑3 (MQ=255)                                                                                   ttAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCa                                                          <  2:725275/141‑1 (MQ=255)                                                                                    tAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCAc                                                         >  1:124029/1‑141 (MQ=255)                                                                                     aGTTGGTATATTATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCAcc                                                        >  1:1050600/1‑141 (MQ=255)                                                                                        tGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTa                                                     >  2:60576/1‑141 (MQ=255)                                                                                           tataTCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTg                                                  <  1:592453/141‑1 (MQ=255)                                                                                            atattatatctttttCGAAGTTGNTTCACATTGTAATCATCGTTTTTCAATTGATATTTTGCGGAATAACTTGGAACAANAGTATTANATGAAATGNCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                 >  1:565595/13‑141 (MQ=9)                                                                                            atatCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                 <  1:105602/141‑1 (MQ=255)                                                                                            atatCATACCTCTTTCTAAGTTGCTTCACAGTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                 >  2:975288/1‑141 (MQ=255)                                                                                               tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                              >  2:948541/1‑141 (MQ=255)                                                                                               tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                              >  2:66626/1‑141 (MQ=255)                                                                                               tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                              >  1:159155/1‑141 (MQ=255)                                                                                                cATACCTCTGTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaa                                             >  1:955571/1‑141 (MQ=255)                                                                                                 aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                            <  2:1030856/141‑1 (MQ=255)                                                                                                 aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                            <  1:137892/141‑1 (MQ=255)                                                                                                     ctctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAt                                        >  2:443322/1‑141 (MQ=255)                                                                                                     atctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAt                                        >  1:1029689/2‑141 (MQ=255)                                                                                                         ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATCATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                    <  1:520836/141‑1 (MQ=255)                                                                                                         ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                    >  1:518574/1‑141 (MQ=255)                                                                                                         ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                    <  2:24184/141‑1 (MQ=255)                                                                                                          tCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaaa                                   >  2:33927/1‑141 (MQ=255)                                                                                                           cTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAg                                  >  1:147351/1‑141 (MQ=255)                                                                                                              aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                               <  2:206335/141‑1 (MQ=255)                                                                                                              aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                               >  1:796993/1‑141 (MQ=255)                                                                                                                 tGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCg                            <  2:2320/141‑1 (MQ=255)                                                                                                                  gCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGa                           <  1:296031/141‑1 (MQ=255)                                                                                                                     tCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAttt                        >  2:822990/1‑141 (MQ=255)                                                                                                                     tCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAttt                        >  1:95081/1‑141 (MQ=255)                                                                                                                       acaTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttct                      >  2:343694/1‑141 (MQ=255)                                                                                                                         aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                    >  2:462576/1‑141 (MQ=255)                                                                                                                         aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                    <  1:909243/141‑1 (MQ=255)                                                                                                                           tGTAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAAtttcttttc                  <  1:614534/141‑1 (MQ=11)                                                                                                                            gTAGTCATCATTACTTTATTGCTACTGTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCTCCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                 <  2:860307/141‑1 (MQ=255)                                                                                                                            gTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                 <  2:492826/141‑1 (MQ=255)                                                                                                                            gTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                 >  2:602475/1‑141 (MQ=255)                                                                                                                            gTAGTCATCATTACTTAATTGATACTATGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                 <  2:860585/141‑1 (MQ=255)                                                                                                                             tAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAATttcttttctt                >  1:350718/1‑141 (MQ=11)                                                                                                                               gTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTaa              <  1:522233/141‑1 (MQ=255)                                                                                                                                 catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt            >  2:1035091/1‑141 (MQ=255)                                                                                                                                 catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt            >  1:409970/1‑141 (MQ=255)                                                                                                                                 catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt            <  1:911426/141‑1 (MQ=255)                                                                                                                                  atcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAttt           >  2:683757/1‑141 (MQ=255)                                                                                                                                  atcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAttt           >  1:890444/1‑141 (MQ=255)                                                                                                                                       tACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTatcat      >  2:361499/1‑141 (MQ=255)                                                                                                                                         cTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTAtcatca    >  2:1012124/1‑141 (MQ=255)                                                                                                                                           tAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGc  <  1:1078515/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                             AAATGTAAACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGC  >  CP000730/2613957‑2614235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑