Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,614,104	C→T	5.2%	K189K (AAG→AAA)	USA300HOU_2478 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,614,104	0	C	T	5.2%	315.5 / 4.1	96	K189K (AAG→AAA)	USA300HOU_2478	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (54/37);  new base T (2/3);  total (56/40)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

ACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTT  >  CP000730/2613965‑2614243                                                                                                                                            |                                                                                                                                            aCTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACt                                                                                                                                            <  2:610952/141‑1 (MQ=255)     tagattttttgacccTACAGATGAGCCGTTTAAATCACCATCACCTTTTATTAATAATTTCGGAGCTTTGTTGGTTGGAATATTATATCTTTTTCGAAGTTGCTTCACATTGTAGTCATCGTTTTTCAATTGATATTTTGc                                                                                                                                        <  2:901543/128‑1 (MQ=255)     tAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGc                                                                                                                                        <  2:64079/141‑1 (MQ=255)      aaaTTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCt                                                                                                                                       <  1:974359/141‑1 (MQ=255)      aaaTTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCt                                                                                                                                       <  2:872945/141‑1 (MQ=255)            ttGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATaa                                                                                                                                 >  2:911426/1‑141 (MQ=255)             tgtgTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAAc                                                                                                                                <  1:709886/141‑1 (MQ=255)                 tCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTgg                                                                                                                            <  2:968325/141‑1 (MQ=255)                        gatgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTc                                                                                                                     <  1:843430/141‑1 (MQ=255)                          tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                                   >  2:1018226/1‑141 (MQ=255)                          tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                                   >  1:969398/1‑141 (MQ=255)                          tgatCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCgg                                                                                                                   >  1:969394/1‑141 (MQ=255)                             tCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGAtt                                                                                                                >  2:719570/1‑141 (MQ=255)                             tCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGAtt                                                                                                                >  1:414551/1‑141 (MQ=255)                              ccTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATta                                                                                                               >  2:23878/1‑141 (MQ=255)                                 tttAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTatat                                                                                                            >  1:1082763/1‑141 (MQ=255)                                  ttAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATg                                                                                                           >  1:534818/1‑141 (MQ=255)                                   tAAATCACCATCGCCTTTTATTAATAATTTATGTGCTTTCTTAGTTTGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGa                                                                                                          <  2:1050642/141‑1 (MQ=255)                                    aaaTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGaa                                                                                                         >  2:984254/1‑141 (MQ=255)                                      aTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAAt                                                                                                       >  2:416811/1‑141 (MQ=255)                                       tCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATg                                                                                                      >  2:199428/1‑141 (MQ=255)                                         aCCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGTACATTCGGATTATATGAAATGTc                                                                                                    >  1:54353/1‑141 (MQ=255)                                          ccATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCa                                                                                                   >  1:720590/1‑141 (MQ=255)                                             tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                                >  2:329677/1‑141 (MQ=255)                                             tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                                <  1:394550/141‑1 (MQ=255)                                               gCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATc                                                                                              <  2:1035806/141‑1 (MQ=255)                                                ccTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCt                                                                                             >  1:1017792/1‑141 (MQ=255)                                                 cTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCtt                                                                                            <  1:1023358/141‑1 (MQ=255)                                                  ttttATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCttt                                                                                           <  1:567498/141‑1 (MQ=255)                                                      attaATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATaa                                                                                       >  2:334074/1‑141 (MQ=255)                                                         aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                                    <  2:351777/141‑1 (MQ=255)                                                         aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                                    <  1:418361/141‑1 (MQ=255)                                                         aataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCa                                                                                    <  2:846648/141‑1 (MQ=255)                                                          ataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCAt                                                                                   <  2:862139/141‑1 (MQ=255)                                                            aatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTa                                                                                 <  1:479228/141‑1 (MQ=255)                                                             atTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTaa                                                                                >  2:244782/1‑141 (MQ=255)                                                               ttAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAata                                                                              >  1:95091/1‑141 (MQ=255)                                                               ttAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAata                                                                              >  1:136658/1‑141 (MQ=255)                                                                  ggTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCt                                                                           >  2:854498/1‑141 (MQ=255)                                                                   gTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCtt                                                                          >  1:306952/1‑141 (MQ=255)                                                                   gTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCtt                                                                          >  2:1043921/1‑141 (MQ=255)                                                                    agCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCttt                                                                         <  2:402280/140‑1 (MQ=21)                                                                      cTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTaa                                                                       <  2:376506/141‑1 (MQ=255)                                                                         tCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTaaatt                                                                    <  1:603127/141‑3 (MQ=255)                                                                           ttAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCa                                                                  <  2:725275/141‑1 (MQ=255)                                                                            tAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCAc                                                                 >  1:124029/1‑141 (MQ=255)                                                                             aGTTGGTATATTATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCAcc                                                                >  1:1050600/1‑141 (MQ=255)                                                                                tGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTa                                                             >  2:60576/1‑141 (MQ=255)                                                                                   tataTCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTg                                                          <  1:592453/141‑1 (MQ=255)                                                                                    atattatatctttttCGAAGTTGNTTCACATTGTAATCATCGTTTTTCAATTGATATTTTGCGGAATAACTTGGAACAANAGTATTANATGAAATGNCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                         >  1:565595/13‑141 (MQ=9)                                                                                    atatCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                         <  1:105602/141‑1 (MQ=255)                                                                                    atatCATACCTCTTTCTAAGTTGCTTCACAGTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGt                                                         >  2:975288/1‑141 (MQ=255)                                                                                       tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                                      >  2:66626/1‑141 (MQ=255)                                                                                       tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                                      >  2:948541/1‑141 (MQ=255)                                                                                       tCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACa                                                      >  1:159155/1‑141 (MQ=255)                                                                                        cATACCTCTGTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaa                                                     >  1:955571/1‑141 (MQ=255)                                                                                         aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                                    <  2:1030856/141‑1 (MQ=255)                                                                                         aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                                    <  1:137892/141‑1 (MQ=255)                                                                                             ctctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAt                                                >  2:443322/1‑141 (MQ=255)                                                                                             atctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAt                                                >  1:1029689/2‑141 (MQ=255)                                                                                                 ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATCATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                            <  1:520836/141‑1 (MQ=255)                                                                                                 ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                            >  1:518574/1‑141 (MQ=255)                                                                                                 ttCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaa                                            <  2:24184/141‑1 (MQ=255)                                                                                                  tCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaaa                                           >  2:33927/1‑141 (MQ=255)                                                                                                   cTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAg                                          >  1:147351/1‑141 (MQ=255)                                                                                                      aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                                       <  2:206335/141‑1 (MQ=255)                                                                                                      aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                                       >  1:796993/1‑141 (MQ=255)                                                                                                         tGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCg                                    <  2:2320/141‑1 (MQ=255)                                                                                                          gCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGa                                   <  1:296031/141‑1 (MQ=255)                                                                                                             tCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAttt                                >  2:822990/1‑141 (MQ=255)                                                                                                             tCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAttt                                >  1:95081/1‑141 (MQ=255)                                                                                                               acaTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttct                              >  2:343694/1‑141 (MQ=255)                                                                                                                 aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                            >  2:462576/1‑141 (MQ=255)                                                                                                                 aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                            <  1:909243/141‑1 (MQ=255)                                                                                                                   tGTAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAAtttcttttc                          <  1:614534/141‑1 (MQ=11)                                                                                                                    gTAGTCATCATTACTTTATTGCTACTGTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCTCCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                         <  2:860307/141‑1 (MQ=255)                                                                                                                    gTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                         >  2:602475/1‑141 (MQ=255)                                                                                                                    gTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                         <  2:492826/141‑1 (MQ=255)                                                                                                                    gTAGTCATCATTACTTAATTGATACTATGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttct                         <  2:860585/141‑1 (MQ=255)                                                                                                                     tAGTCATTGTTACTCAATTGATATTTTGCGGAATAACTTGGAACATTAGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAAAACTTAAAGTCTTCAATttcttttctt                        >  1:350718/1‑141 (MQ=11)                                                                                                                       gTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTaa                      <  1:522233/141‑1 (MQ=255)                                                                                                                         catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                    >  1:409970/1‑141 (MQ=255)                                                                                                                         catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                    <  1:911426/141‑1 (MQ=255)                                                                                                                         catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                    >  2:1035091/1‑141 (MQ=255)                                                                                                                          atcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAttt                   >  2:683757/1‑141 (MQ=255)                                                                                                                          atcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAttt                   >  1:890444/1‑141 (MQ=255)                                                                                                                               tACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTatcat              >  2:361499/1‑141 (MQ=255)                                                                                                                                 cTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTAtcatca            >  2:1012124/1‑141 (MQ=255)                                                                                                                                   tAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGc          <  1:1078515/141‑1 (MQ=255)                                                                                                                                    aaTTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCt         >  2:12694/1‑141 (MQ=255)                                                                                                                                    aaTTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCt         >  1:658002/1‑141 (MQ=255)                                                                                                                                         aTACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCgg    >  1:584108/1‑141 (MQ=255)                                                                                                                                          tACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGt   >  1:804759/1‑141 (MQ=255)                                                                                                                                           aCTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGtt  >  2:355830/1‑141 (MQ=255)                                                                                                                                           aCTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGtt  >  2:355918/1‑141 (MQ=255)                                                                                                                                           aCTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGtt  <  2:534818/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                            ACTCTAAATTTTTGTGTCCTATAGATGATCCTTTTAAATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTT  >  CP000730/2613965‑2614243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑