Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 51.6% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC51.6% 42.2 / inf 595intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (160/128);  new base C (179/127);  total (339/256)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 597 total aligned reads displayed.

AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGG  >  CP000730/76158‑76432
                                                                                                                                         |                                                                                                                                          
                    atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                     >  1:322675/4‑141 (MQ=2)
                    agcgcaaccataACTTGAACAAAAAGGACTCTTCTGTATGAACTATTTCCGCTATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt                                                                                                                     >  2:762733/10‑141 (MQ=11)
                     tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                                                    >  2:92869/3‑141 (MQ=14)
                     gtagaccgttgaatgatgaacgtttacaccacgttcccttaaTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                                                    >  1:563615‑M1/43‑141 (MQ=255)
                      ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                   <  1:339964/140‑1 (MQ=12)
                      cTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa                                                                                                                   <  1:6748/141‑1 (MQ=33)
                        ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTACCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                                                 <  1:478514/141‑2 (MQ=255)
                         taatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgag                                                                                                                <  2:780225/138‑3 (MQ=12)
                            tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAc                                                                                                             <  1:723763/141‑1 (MQ=17)
                            tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAc                                                                                                             <  1:948603/141‑1 (MQ=12)
                             ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACg                                                                                                            >  1:843379/1‑141 (MQ=17)
                              taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                                           <  2:361993/141‑1 (MQ=17)
                              taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                                           >  1:819627/1‑141 (MQ=12)
                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                                         >  1:1084970/1‑141 (MQ=17)
                                     aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                                                    >  1:89305/1‑141 (MQ=17)
                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                                   <  1:499248/141‑1 (MQ=12)
                                        aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                                                 <  2:113646/141‑1 (MQ=12)
                                        aaaaaGGAGTCTTCAGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACTTGGTGTAAACg                                                                                                 >  2:983305/1‑141 (MQ=255)
                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                              <  2:850129/141‑1 (MQ=17)
                                            aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtca                                                                                             <  2:537805/141‑1 (MQ=12)
                                               aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                          <  2:916957/141‑1 (MQ=17)
                                               aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                          >  2:336226/1‑141 (MQ=12)
                                                 tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                        >  2:148112/1‑141 (MQ=17)
                                                  cttctGTATGAACTATTTCAGATATAACCAATTTAACACGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                                                       <  1:1006306/141‑1 (MQ=255)
                                                   ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCa                                                                                      <  2:830111/141‑1 (MQ=17)
                                                    tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCaa                                                                                     <  1:610107/141‑1 (MQ=17)
                                                    tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCaa                                                                                     <  2:1006266/141‑1 (MQ=12)
                                                      tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                   <  2:668827/141‑1 (MQ=12)
                                                       gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                  >  2:318159/1‑141 (MQ=17)
                                                        tATGAACTATTTCAGATATAAACACTTTAACAAGGATGTTATCACTGTAGCAGTTGGCTACTCTCTAAGATACGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgat                                                                                 <  1:381658/141‑3 (MQ=11)
                                                          tgaaaTATTTCAGAGATAAACAAGTTAAAAAGGATGTTATCACTGTAGCCGTTGGCTACTATATAAGATATGCATTGAGCTGTCGTGATATATCTGAAATAGTAAGAGAAGGAGGTGTCAACGTTCATCATTCAACGATCt                                                                               <  2:598983‑M1/136‑1 (MQ=255)
                                                            aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGtagac                                                                             <  1:150050/141‑5 (MQ=17)
                                                            aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                             >  2:431609/1‑141 (MQ=12)
                                                             aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGATCGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                            <  2:103237/141‑1 (MQ=17)
                                                             aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                            <  1:980083/141‑1 (MQ=255)
                                                             aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATACTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                            <  1:935125/141‑1 (MQ=11)
                                                                ATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                         <  1:421005‑M1/141‑1 (MQ=255)
                                                                ATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                         <  2:223475‑M1/141‑1 (MQ=255)
                                                                 TTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                                                        <  1:791983‑M1/141‑1 (MQ=255)
                                                                 TTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                                                        <  1:657518‑M1/141‑1 (MQ=255)
                                                                 TTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATATACCGTTg                                                                        >  1:292322‑M1/1‑141 (MQ=255)
                                                                   TCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                      <  1:755238‑M1/141‑1 (MQ=255)
                                                                       ATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                  >  1:25600‑M1/1‑141 (MQ=255)
                                                                       ATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                  <  2:170502‑M1/141‑1 (MQ=255)
                                                                       ATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                  <  1:131594‑M1/141‑1 (MQ=255)
                                                                         ATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                >  2:351052‑M1/1‑141 (MQ=255)
                                                                         ATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                >  2:962013‑M1/1‑141 (MQ=255)
                                                                          TAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                               <  2:633715‑M1/141‑1 (MQ=255)
                                                                            aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                             >  1:926144/1‑141 (MQ=12)
                                                                             aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAata                                                            >  1:1037283/1‑141 (MQ=17)
                                                                                aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGGACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                         >  2:789721/1‑141 (MQ=17)
                                                                                  ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                       >  1:706695/1‑141 (MQ=255)
                                                                                    taCAAGGATGTTATTACTGTAGCCGTTGGCTATTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTTAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                     >  2:482403/2‑141 (MQ=11)
                                                                                    aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                     >  2:120838/1‑141 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                    >  2:510334/1‑141 (MQ=17)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                    >  2:803747/1‑141 (MQ=32)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                    >  1:920581/1‑141 (MQ=32)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                    >  1:167291/1‑141 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                    >  2:282042/1‑141 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGCTGGGTTCAAGAATATGCCCCaa                                                    >  2:414225/1‑141 (MQ=255)
                                                                                      cAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGGTCAAGAATATGCCCCAAt                                                   >  1:585658/1‑141 (MQ=17)
                                                                                      cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                   >  2:754793/1‑141 (MQ=255)
                                                                                        aGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                 <  1:399812/141‑1 (MQ=17)
                                                                                         ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                <  1:538292/141‑1 (MQ=255)
                                                                                          gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                                              <  2:735696/62‑1 (MQ=255)
                                                                                          gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                               >  1:360019/1‑141 (MQ=18)
                                                                                            tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGGAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAATAATATGCCCCAATTTtata                                             <  1:526528/141‑1 (MQ=255)
                                                                                            tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                             <  1:229982/141‑1 (MQ=25)
                                                                                            tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                             <  2:953364/141‑1 (MQ=21)
                                                                                            tGTTATCACTGTAGCCGTTTGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                             <  1:772909/141‑1 (MQ=255)
                                                                                             gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                            <  2:1058300/141‑1 (MQ=25)
                                                                                             gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                            <  1:308038/141‑1 (MQ=25)
                                                                                             gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                            >  1:212099/1‑141 (MQ=255)
                                                                                             gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                            >  1:957243/1‑141 (MQ=255)
                                                                                              ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                           <  2:868066/141‑1 (MQ=255)
                                                                                              ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                           <  1:1021586/141‑1 (MQ=255)
                                                                                               tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                          <  2:684117/141‑1 (MQ=255)
                                                                                                   aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt                                      >  2:400212/1‑141 (MQ=255)
                                                                                                      gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                   >  2:569739/1‑141 (MQ=35)
                                                                                                        aGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATTTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATCTTATATCAAATTTGgaa                                 >  1:878825/1‑141 (MQ=255)
                                                                                                         gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                >  1:573853/1‑141 (MQ=255)
                                                                                                         gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACGATCTACCGGTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                >  1:648754/1‑141 (MQ=11)
                                                                                                            gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTAGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                             >  1:457167/1‑141 (MQ=11)
                                                                                                            aacggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                             >  1:6306/4‑141 (MQ=25)
                                                                                                             ttGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaa                            >  1:186953/1‑141 (MQ=255)
                                                                                                              tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATAAAAATAAGAAGaaaaa                           >  2:856481/1‑141 (MQ=255)
                                                                                                               ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                          >  1:1072747/1‑141 (MQ=255)
                                                                                                               ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                          >  1:693273/1‑141 (MQ=255)
                                                                                                               ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                          >  1:1046116/1‑141 (MQ=255)
                                                                                                                  tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACTTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAACGCAt                       >  2:1073684/1‑141 (MQ=21)
                                                                                                                     taTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                   >  2:837321/1‑141 (MQ=35)
                                                                                                                     taTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                   <  1:438001/141‑1 (MQ=35)
                                                                                                                     taTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                   >  2:650801/1‑141 (MQ=255)
                                                                                                                       tCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                 <  1:740187/141‑1 (MQ=35)
                                                                                                                           aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct             >  2:238449/1‑137 (MQ=11)
                                                                                                                            gATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctt            <  2:893386/141‑6 (MQ=11)
                                                                                                                              tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat          >  2:183244/1‑134 (MQ=11)
                                                                                                                                   aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACaaa     <  2:499035/141‑1 (MQ=39)
                                                                                                                                   aTTGAGTTATCGTGATATAACTGAAATATTAAGGGAACGTGGAGTAAACGTTCATCATACAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATAACAAATTTGGAAGAAAAAGCATAAAAAAGATAATTACaa      >  2:376037/1‑141 (MQ=1)
                                                                                                                                      gAGTTATCGTGATATATCTGAAATATTAATGGAGCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATgg  <  2:589499/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGG  >  CP000730/76158‑76432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: