Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,622,662 A→G 5.9% Y3Y (TAT→TAC USA300HOU_2485 ← MFS family major facilitator transporter, cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,622,6620AG 5.9% 253.4 / 5.0 85Y3Y (TAT→TACUSA300HOU_2485MFS family major facilitator transporter, cation symporter
Reads supporting (aligned to +/- strand):  ref base A (33/47);  new base G (3/2);  total (36/49)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GATAATTTTAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATA  >  CP000730/2622525‑2622802
                                                                                                                                         |                                                                                                                                             
aaTAATTTTAAATATGATTGGATTGTAAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATac                                                                                                                                            <  2:758549/140‑1 (MQ=255)
         aaaGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAttt                                                                                                                                   <  2:1005660/141‑1 (MQ=255)
          aaGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTa                                                                                                                                  <  1:913041/141‑1 (MQ=255)
           aGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTAt                                                                                                                                 >  1:271678/1‑141 (MQ=255)
           aGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTAt                                                                                                                                 >  1:500366/1‑141 (MQ=255)
            gatgatTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACACAACGACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATc                                                                                                                                <  1:565473/141‑1 (MQ=255)
               gatTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCAcc                                                                                                                             >  1:406134/1‑141 (MQ=255)
                    gTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCa                                                                                                                        >  2:347694/1‑141 (MQ=255)
                    gTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCa                                                                                                                        <  2:420490/141‑1 (MQ=255)
                        gTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATaaa                                                                                                                    >  2:681847/1‑141 (MQ=255)
                          cAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATaaaaa                                                                                                                  <  2:126841/141‑1 (MQ=255)
                          cAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCACTCATaaaaa                                                                                                                  >  1:1077455/1‑141 (MQ=255)
                             ttCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAaataa                                                                                                               >  1:670769/1‑141 (MQ=255)
                                aaCAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTg                                                                                                            <  1:335535/141‑1 (MQ=255)
                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGaa                                                                                                          >  2:297464/1‑141 (MQ=255)
                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGaa                                                                                                          <  1:462026/141‑1 (MQ=255)
                                  cAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGaa                                                                                                          <  2:807402/141‑1 (MQ=255)
                                   aGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAAt                                                                                                         <  1:272204/141‑1 (MQ=255)
                                    gTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTTTTATCTTCTAAATACAACATATTTATTACCTCTTATAAAAATAATTTAAta                                                                                                        >  2:749048/1‑140 (MQ=255)
                                     tAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGc                                                                                                       <  1:540153/141‑1 (MQ=255)
                                     tAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGc                                                                                                       >  1:816343/1‑141 (MQ=255)
                                           aaaTTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccac                                                                                                 <  1:319598/141‑1 (MQ=255)
                                           aaaTTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccac                                                                                                 <  1:98477/141‑1 (MQ=255)
                                            aaTTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccacc                                                                                                >  1:1015212/1‑141 (MQ=255)
                                             attattTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATccacca                                                                                               <  1:241445/141‑1 (MQ=255)
                                               tattTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGc                                                                                            <  2:579396/141‑1 (MQ=255)
                                                 tttGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCtt                                                                                          <  1:112257/141‑1 (MQ=255)
                                                    gTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCttttt                                                                                       >  1:176949/1‑141 (MQ=255)
                                                    gTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCttttt                                                                                       <  1:402624/141‑1 (MQ=255)
                                                    gTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCttttt                                                                                       <  1:476265/141‑1 (MQ=255)
                                                     tAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCtttttt                                                                                      >  1:694734/1‑141 (MQ=255)
                                                         gAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGAc                                                                                  >  2:703628/1‑141 (MQ=255)
                                                           aaaCAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACct                                                                                <  1:790486/141‑1 (MQ=255)
                                                            aaCAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACctt                                                                               <  1:533085/141‑1 (MQ=255)
                                                            aaCAGCACAAAACAACTCCGACGTAAGAAACTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACctt                                                                               >  1:201454/1‑141 (MQ=255)
                                                                gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCttcttc                                                                           >  2:396784/1‑141 (MQ=255)
                                                                gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCttcttc                                                                           <  2:371064/141‑1 (MQ=255)
                                                                  acaAAGCAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTtcttct                                                                          <  1:84166/141‑1 (MQ=255)
                                                                  acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTa                                                                         <  2:318480/141‑1 (MQ=255)
                                                                  acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTCAATGCATCCACCAGCTTTTTTAGACCTTCTTCTa                                                                         >  2:843895/1‑141 (MQ=255)
                                                                      agcaacTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAAc                                                                      >  1:440213/3‑141 (MQ=255)
                                                                          acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAActctct                                                                 >  2:10433/1‑141 (MQ=255)
                                                                          acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAActctct                                                                 <  1:208387/141‑1 (MQ=255)
                                                                          acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAActctct                                                                 >  2:81073/1‑141 (MQ=255)
                                                                                 cGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCa                                                          >  1:808307/1‑141 (MQ=255)
                                                                                    aaGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAg                                                       <  2:680749/141‑1 (MQ=255)
                                                                                      aaaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcg                                                     <  1:569508/140‑1 (MQ=255)
                                                                                       aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                    <  1:683980/141‑1 (MQ=255)
                                                                                       aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                    <  2:408240/141‑1 (MQ=255)
                                                                                       aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAGTTATCACCTCTCATAAGAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                    <  2:459379/141‑1 (MQ=255)
                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaa                                                  >  1:124994/1‑141 (MQ=255)
                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                 <  1:946265/141‑1 (MQ=255)
                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                 <  1:324452/141‑1 (MQ=255)
                                                                                          ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                 <  1:297852/141‑1 (MQ=255)
                                                                                               aatcaatAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaattaatt                                            >  1:79376/1‑141 (MQ=255)
                                                                                                 tcaatAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCt                                          <  2:592932/141‑1 (MQ=255)
                                                                                                   aataaataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTaa                                        <  2:787519/141‑1 (MQ=255)
                                                                                                     taaataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAata                                      <  1:746963/141‑1 (MQ=255)
                                                                                                       aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAataa                                     >  1:740635/1‑140 (MQ=255)
                                                                                                       aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAAtata                                    >  2:453281/1‑141 (MQ=255)
                                                                                                       aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAAtata                                    <  2:591771/141‑1 (MQ=255)
                                                                                                          atGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAAtt                                 <  1:437884/141‑1 (MQ=255)
                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCTACCACCTATCCTCGACATTCTTCTACACTCTCTTTATCAAAAACGCCATTAATTCTAATATAATTTaa                              >  2:672031/1‑140 (MQ=255)
                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                              >  2:872120/1‑141 (MQ=255)
                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                              <  1:87416/141‑1 (MQ=255)
                                                                                                             tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                              <  1:703628/141‑1 (MQ=255)
                                                                                                                    tCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTa                       <  1:966559/141‑1 (MQ=255)
                                                                                                                      tATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATAAAATTAAGTCAGTTaa                      <  1:7775/141‑1 (MQ=255)
                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                     <  1:438497/141‑1 (MQ=255)
                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                     >  1:471751/1‑141 (MQ=255)
                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                     >  1:706897/1‑141 (MQ=255)
                                                                                                                      tATTTTTCAAAACTTCTAAATACAACATATTTATCACCGCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATAAACTTTAGTCAGTTaaa                     >  2:236559/1‑141 (MQ=255)
                                                                                                                       aTTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTTTTTTTTTTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAat                    >  2:402296/1‑141 (MQ=255)
                                                                                                                        tttttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGCCATTTAGGTGAACTCTCTTTATCCAAAGCGCACTTAATTCTAATATAATTTAGTCATTTAAata                   >  1:836145/1‑141 (MQ=255)
                                                                                                                        tttttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAata                   >  1:176987/1‑141 (MQ=255)
                                                                                                                          tttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATc                 <  1:599164/141‑1 (MQ=255)
                                                                                                                          tttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATc                 <  2:694734/141‑1 (MQ=255)
                                                                                                                             cAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAat              <  1:317751/141‑1 (MQ=255)
                                                                                                                             cAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAat              <  2:454798/141‑1 (MQ=255)
                                                                                                                                aaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattat           >  1:72357/1‑141 (MQ=255)
                                                                                                                                aaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattat           >  1:72368/1‑141 (MQ=255)
                                                                                                                                 aCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattatt          >  1:1079574/1‑141 (MQ=255)
                                                                                                                                    tCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCg       <  1:579539/141‑1 (MQ=255)
                                                                                                                                     cTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGa      <  2:68913/141‑1 (MQ=255)
                                                                                                                                     cTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGa      <  2:375442/141‑1 (MQ=255)
                                                                                                                                         aTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAata  >  2:808325/1‑141 (MQ=255)
                                                                                                                                         aTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAata  >  1:1029786/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                             
GATAATTTTAAAGATGATTGGTTTGTCAATTCAACAGTAAACCAAATTATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATA  >  CP000730/2622525‑2622802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: