BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,653,181	A→G	5.5%	L584L (CTA→CTG) ‡	fbp →	fructose‑bisphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,653,181	0	A	G	5.5%	312.4 / 7.1	109	L584L (CTA→CTG) ‡	fbp	fructose‑bisphosphatase
Reads supporting (aligned to +/- strand): ref base A (55/46); new base G (2/4); total (59/50)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 110 total aligned reads displayed.

ACGCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATAC  >  CP000730/2653043‑2653321

aCGCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTAtt                                                                                                                                            >  2:635563/1‑141 (MQ=255)

  gCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGt                                                                                                                                          <  1:917188/141‑1 (MQ=255)

    attattAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGtat                                                                                                                                        >  1:150079/1‑141 (MQ=255)

       attaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAat                                                                                                                                     <  2:532245/141‑1 (MQ=255)

        ttaATGGTCACACACCAGTGAACGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                    >  2:545756/1‑141 (MQ=255)

        ttaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAATCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                    <  1:679876/141‑1 (MQ=255)

        ttaATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                    >  1:1065637/1‑141 (MQ=255)

        ttaATGGTCACACACCAGAGAAAGCAAGCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCCACAACTGTCATTGCAGGCTATACACTATTGTATAatt                                                                                                                                    >  2:545794/1‑141 (MQ=255)

           aTGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATAAACTATTGTATAattcat                                                                                                                                 >  2:281124/1‑141 (MQ=255)

            tGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAattcatt                                                                                                                                >  1:93817/1‑141 (MQ=255)

                 acacacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTa                                                                                                                           >  1:716817/1‑141 (MQ=255)

                   acacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATg                                                                                                                         <  2:277370/141‑1 (MQ=255)

                    cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                        <  1:1087215/141‑1 (MQ=255)

                    cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                        <  2:494143/141‑1 (MQ=255)

                    cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                        <  1:452626/141‑1 (MQ=255)

                    cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTAAAATTCATTCGGTATGc                                                                                                                        >  1:739652/1‑141 (MQ=255)

                        aGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACt                                                                                                                    >  1:383810/1‑141 (MQ=255)

                         gTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATAGCAGGCTATACACTCTTGTATAATTCATTCGGTATGCAACTc                                                                                                                   >  2:357386/1‑141 (MQ=255)

                          tGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTGTGCAAcgag                                                                                                                  <  1:922079/141‑4 (MQ=255)

                          tGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCg                                                                                                                  >  2:253318/1‑141 (MQ=255)

                          tGAAAGAAAGCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTGTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTGTGCAAcgag                                                                                                                  <  1:675484/141‑4 (MQ=255)

                            aaagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGtt                                                                                                                >  1:527532/1‑141 (MQ=255)

                             aagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACCCTATCGTATAATTCATTCGGTGTGCAAcgagttg                                                                                                               <  2:322833/141‑7 (MQ=255)

                              agaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGc                                                                                                              >  1:430943/1‑141 (MQ=255)

                               gaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGca                                                                                                             >  1:477644/1‑141 (MQ=255)

                                aaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcac                                                                                                            <  2:841308/141‑1 (MQ=255)

                                aaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcac                                                                                                            <  2:841181/141‑1 (MQ=255)

                                 aaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCATCTCGTTGcaca                                                                                                           <  2:1065244/141‑1 (MQ=255)

                                 aaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcaca                                                                                                           <  2:506950/141‑1 (MQ=255)

                                  aTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCacac                                                                                                          >  1:821402/1‑141 (MQ=255)

                                     aaTGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACcaa                                                                                                       >  2:511657/1‑141 (MQ=255)

                                     aaTGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCAGTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACcaa                                                                                                       <  1:855332/141‑1 (MQ=255)

                                              gATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATCCGGTATGCAACTCGTTGCACACCAACAATTTAAc                                                                                              >  1:1007621/1‑141 (MQ=255)

                                                tCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGc                                                                                            <  1:396524/141‑1 (MQ=255)

                                                tCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGc                                                                                            <  1:184240/141‑1 (MQ=255)

                                                 ccTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCt                                                                                           >  1:650702/1‑141 (MQ=255)

                                                 ccTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGCATGCAACTCGTTGCACACCAACAATTTAACGCt                                                                                           <  1:563831/141‑1 (MQ=255)

                                                  cTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTa                                                                                          >  1:112038/1‑141 (MQ=255)

                                                    aTCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTaaa                                                                                        >  1:841541/1‑141 (MQ=255)

                                                      cAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGa                                                                                      >  2:878024/1‑141 (MQ=255)

                                                        aGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTAAAAAAAAAAAAAATCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACTCCAACTATTTaaaaaaaaaaaaa                                                                                    <  2:965732/141‑12 (MQ=255)

                                                          gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                  >  1:139449/1‑141 (MQ=255)

                                                          gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                  >  2:1073480/1‑141 (MQ=255)

                                                          gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                  >  1:994893/1‑141 (MQ=255)

                                                           cTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGATAAAGaaaaaa                                                                                 <  2:183470/141‑1 (MQ=255)

                                                             gATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATa                                                                               >  1:389700/1‑141 (MQ=255)

                                                             gATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCAGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATa                                                                               <  2:739869/141‑1 (MQ=255)

                                                              aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                              <  1:607764/141‑1 (MQ=255)

                                                              aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                              <  1:598439/141‑1 (MQ=255)

                                                              aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                              >  2:401083/1‑141 (MQ=255)

                                                               tGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACt                                                                             <  2:783879/141‑1 (MQ=255)

                                                                  aaaGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTc                                                                          <  1:295965/141‑1 (MQ=255)

                                                                     gATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGa                                                                       >  1:1069693/1‑141 (MQ=255)

                                                                      aTGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGaa                                                                      <  1:893134/141‑1 (MQ=255)

                                                                            gTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATc                                                                >  1:914229/1‑141 (MQ=255)

                                                                              cATTGATGATGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGa                                                              <  2:1024374/141‑1 (MQ=255)

                                                                               attgccggtggCTTTTCAAAAGCTTATCAGGCAACAACTGGCATGGCAGGCTATACACGATTGTATAATTCATTCGGTAGGCAACTCGTTGCACACAAACAATTTAACGCTAATGAAAAATTACTTTCCGAAGATAACGAt                                                             >  2:317162/7‑141 (MQ=255)

                                                                                   aTGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAAc                                                         >  2:123607/1‑141 (MQ=255)

                                                                                    tggtggTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGGACGCAACTCGTTGCACACCAACAATTTAACGCTAAAGGAAAAATACTTTCCGAAGGTATCGATGAACt                                                        <  1:289496/141‑1 (MQ=255)

                                                                                    tggtggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTCAAGAAAAAATACTTTCCGAAGGTATCGATGAACt                                                        >  1:1005230/1‑141 (MQ=255)

                                                                                        ggtttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                    >  1:718876/4‑141 (MQ=255)

                                                                                        ggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                    >  2:974611/1‑141 (MQ=255)

                                                                                        ggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                    >  2:700321/1‑141 (MQ=255)

                                                                                           ttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCtata                                                 >  2:197991/1‑141 (MQ=255)

                                                                                           ttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCtata                                                 >  2:198053/1‑141 (MQ=255)

                                                                                              tCAAAAGCTTATCAGTCAGCAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAg                                              <  2:540347/141‑1 (MQ=255)

                                                                                               cAAAAGCTTATCAGTCAACAACTGACATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgc                                             >  2:896439/1‑141 (MQ=255)

                                                                                                aaaaGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                            <  2:210399/141‑1 (MQ=255)

                                                                                                   aGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgt                                         >  2:478865/1‑141 (MQ=255)

                                                                                                     cTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcg                                       >  1:961169/1‑141 (MQ=255)

                                                                                                     cTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcg                                       <  1:527124/141‑1 (MQ=255)

                                                                                                      ttATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcgt                                      >  2:431235/1‑141 (MQ=255)

                                                                                                       tATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTa                                     <  2:841541/141‑1 (MQ=255)

                                                                                                        aTCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAg                                    >  1:359924/1‑141 (MQ=255)

                                                                                                          cAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAATTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGAt                                  <  1:520614/141‑1 (MQ=255)

                                                                                                           aGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATa                                 <  1:368805/141‑1 (MQ=255)

                                                                                                           aGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATa                                 >  1:469566/1‑141 (MQ=255)

                                                                                                              caacaaCTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAg                              <  1:253318/141‑1 (MQ=255)

                                                                                                              caacaaCTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAg                              >  2:875976/1‑141 (MQ=255)

                                                                                                               aacaacTGGCATTGCAGGCTATACACTCTTGTATAATTCATTCGGTATGCAACTCGTTGACCACCAACACTACCACGCTAATGATAAAATCCTTCCCGAAGGTATCGAGGAACTTTCTATAAAGCGCGTCGTAGATAAAGa                             >  1:317664/1‑141 (MQ=255)

                                                                                                                acaacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGaa                            <  2:1047793/141‑1 (MQ=255)

                                                                                                                 caacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAAt                           <  2:118045/141‑1 (MQ=255)

                                                                                                                 caacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCATCGTAGATAAAGAAt                           >  1:900350/1‑141 (MQ=255)

                                                                                                                      ggCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACaa                      >  1:689036/1‑141 (MQ=255)

                                                                                                                           tGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaa                 >  1:63631/1‑141 (MQ=255)

                                                                                                                           tGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaa                 <  2:199961/141‑1 (MQ=255)

                                                                                                                             caagCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTTGTAGACAAAGAATTACAACGTaaga               <  2:450374/138‑1 (MQ=255)

                                                                                                                             aaGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTTGTAGACAAAGAATTACAACGTaaga               <  2:450102/140‑1 (MQ=255)

                                                                                                                              aGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaagaa              <  2:506605/141‑1 (MQ=255)

                                                                                                                               ggCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaagaag             >  2:485697/1‑141 (MQ=255)

                                                                                                                               ggCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaagaag             <  1:351205/141‑1 (MQ=255)

                                                                                                                                gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga            >  2:639607/1‑141 (MQ=255)

                                                                                                                                gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga            >  2:886529/1‑141 (MQ=255)

                                                                                                                                   ataCACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTc         >  2:1041701/1‑141 (MQ=255)

                                                                                                                                      cacTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                            <  1:779960/103‑1 (MQ=255)

                                                                                                                                        cTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGAt    <  2:590331/141‑1 (MQ=255)

                                                                                                                                         tATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATa   <  1:804171/141‑1 (MQ=255)

                                                                                                                                         tATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATa   <  2:177683/141‑1 (MQ=255)

                                                                                                                                         tATTGTATAATTCATCCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATa   <  2:430943/141‑1 (MQ=255)

                                                                                                                                          aTTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAACTTAACGCTAAAGAAAAAATACTTTCCGAAGGGATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATAc  <  1:101993/141‑1 (MQ=255)

ACGCATTATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATAC  >  CP000730/2653043‑2653321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`