Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,690,960 T→C 5.8% T559T (ACA→ACG USA300HOU_2546 ← RND superfamily resistance‑nodulation‑cell division:proton (H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,690,9600TC 5.8% 304.5 / 6.8 103T559T (ACA→ACGUSA300HOU_2546RND superfamily resistance‑nodulation‑cell division:proton (H+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (45/52);  new base C (4/2);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 105 total aligned reads displayed.

GACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAGA  >  CP000730/2690822‑2691100
                                                                                                                                          |                                                                                                                                            
gACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGt                                                                                                                                            >  1:626388/1‑141 (MQ=255)
 aCTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGtt                                                                                                                                           <  1:328118/141‑1 (MQ=255)
 aCTTCATAAGCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGtt                                                                                                                                           <  1:1055927/141‑1 (MQ=255)
   ttCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGc                                                                                                                                         <  1:736075/141‑1 (MQ=255)
    tCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGcc                                                                                                                                        >  1:517558/1‑141 (MQ=255)
    tCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGcc                                                                                                                                        >  1:43743/1‑141 (MQ=255)
           ccATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATg                                                                                                                                 >  1:177699/1‑141 (MQ=255)
            cATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGa                                                                                                                                >  1:732773/1‑141 (MQ=255)
             aTCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGAc                                                                                                                               <  2:601342/141‑1 (MQ=255)
              tCGCTAATCCAAATAAGATACCTCTAGTAATAANCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACAAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTANTGACa                                                                                                                              >  1:355329/1‑141 (MQ=255)
              tCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACa                                                                                                                              >  2:637438/1‑141 (MQ=255)
              tCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACa                                                                                                                              <  1:778815/141‑1 (MQ=255)
                   aaTCCAAATAATTTACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTCATGTTGCCATTCATGACCAGACg                                                                                                                         <  2:753604/141‑1 (MQ=255)
                   aaTCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACg                                                                                                                         >  1:579502/1‑141 (MQ=255)
                   aaTCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACg                                                                                                                         >  1:603381/1‑141 (MQ=255)
                   aaTCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACg                                                                                                                         <  2:337359/141‑1 (MQ=255)
                         aaTAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCt                                                                                                                   >  1:744828/1‑141 (MQ=255)
                           tAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTaa                                                                                                                 <  2:139547/141‑1 (MQ=255)
                             aGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGAAAAGACAAATCCTaaaa                                                                                                               >  2:58441/1‑141 (MQ=255)
                               aTACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACt                                                                                                             <  2:439936/141‑1 (MQ=255)
                                tACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTg                                                                                                            <  2:515518/141‑1 (MQ=255)
                                 aCCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGc                                                                                                           >  1:456032/1‑141 (MQ=255)
                                 aCCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGc                                                                                                           <  2:188297/141‑1 (MQ=255)
                                  ccTATATCAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCt                                                                                                          <  2:753798/141‑1 (MQ=255)
                                   cTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCtt                                                                                                         <  2:609341/141‑1 (MQ=255)
                                      taGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTa                                                                                                      <  1:822625/141‑1 (MQ=255)
                                      taGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTa                                                                                                      <  2:738966/141‑1 (MQ=255)
                                          aataaCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTaacgg                                                                                                  >  1:426287/1‑138 (MQ=255)
                                          aataaCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATgg                                                                                                  <  2:1686/141‑1 (MQ=255)
                                             aaCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTCAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTAt                                                                                               >  1:431667/1‑141 (MQ=255)
                                              aCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTAtt                                                                                              >  2:833942/1‑141 (MQ=255)
                                                cGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCACTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAg                                                                                            <  1:1081698/141‑1 (MQ=255)
                                                 ggTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGa                                                                                           <  1:696975/141‑1 (MQ=255)
                                                  gTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGaa                                                                                          >  2:1009333/1‑141 (MQ=255)
                                                   tAAAAATGCTAGCATTGGTCATGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTAACGGTATTAGAAt                                                                                         <  2:804331/141‑1 (MQ=255)
                                                        aTGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGaaa                                                                                    >  1:144154/1‑140 (MQ=255)
                                                         tGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCGAGAACTGCTTTTAATGGTATTAGAATTGAACg                                                                                   <  1:14675/141‑1 (MQ=255)
                                                           cTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGa                                                                                 >  2:424065/1‑141 (MQ=255)
                                                           cTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTGTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGa                                                                                 >  1:844048/1‑141 (MQ=255)
                                                            tAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGaa                                                                                >  2:925548/1‑141 (MQ=255)
                                                             aGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAg                                                                               >  2:179083/1‑141 (MQ=255)
                                                                 ttGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACaa                                                                           <  1:912975/141‑1 (MQ=255)
                                                                  tGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAAt                                                                          <  2:1015976/141‑1 (MQ=255)
                                                                    gTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAACACAATCa                                                                        <  1:861009/141‑1 (MQ=255)
                                                                     tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTAACGGTATTAGAATTGAACGGAAGACAATCAt                                                                       >  2:746384/1‑141 (MQ=255)
                                                                     tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                       <  1:78064/141‑1 (MQ=255)
                                                                     tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                       >  1:730468/1‑141 (MQ=255)
                                                                     tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                       >  1:190950/1‑141 (MQ=255)
                                                                     tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                       >  1:986970/1‑141 (MQ=255)
                                                                     tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAAAGGAAGACAATCAt                                                                       >  2:864061/1‑141 (MQ=255)
                                                                        tGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATtaa                                                                    <  2:171477/141‑1 (MQ=255)
                                                                         gTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATtaat                                                                   <  1:822036/141‑1 (MQ=255)
                                                                           cGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAAtaaaaaaaaa                                                                 <  2:704001/141‑10 (MQ=255)
                                                                               tCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGaaa                                                             >  2:183996/1‑141 (MQ=255)
                                                                               tCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGaaa                                                             >  2:716799/1‑141 (MQ=255)
                                                                                cAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGaaaa                                                            >  2:821779/1‑141 (MQ=255)
                                                                                cAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATAAATAAGaaaa                                                            <  1:458343/141‑1 (MQ=255)
                                                                                cAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAATAATAAGaaaa                                                            <  2:779100/141‑1 (MQ=255)
                                                                                  aTACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTCTTAGAATTGAACGGAAGACAATCATTAATAAGAAAAAt                                                          >  1:751242/1‑141 (MQ=255)
                                                                                    aCCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGc                                                        <  2:107695/141‑1 (MQ=255)
                                                                                     ccAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCt                                                       >  1:820008/1‑141 (MQ=255)
                                                                                     ccAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTCTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCt                                                       >  1:962804/1‑141 (MQ=255)
                                                                                      cAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTa                                                      <  1:449654/141‑1 (MQ=255)
                                                                                       aaaCAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTaa                                                     <  2:656057/141‑1 (MQ=255)
                                                                                         aCAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATa                                                   <  1:1015115/141‑1 (MQ=255)
                                                                                         aCAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATa                                                   <  1:115847/141‑1 (MQ=255)
                                                                                             aCCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATacaac                                               <  2:1035629/141‑1 (MQ=255)
                                                                                              ccTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATacaaca                                              >  1:681903/1‑141 (MQ=255)
                                                                                               ttttCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATaccacac                                             <  2:435721/140‑2 (MQ=255)
                                                                                               cTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATAcaacaa                                             >  2:911842/1‑141 (MQ=255)
                                                                                                 ttCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATg                                           >  1:100213/1‑141 (MQ=255)
                                                                                                      aaaaCCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTAACGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCCAATACAACAATTACTgt                                      >  1:860561/1‑140 (MQ=255)
                                                                                                      aaaaCCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATAAAAAAGAAAAATGCTAATACAACAATGACTGa                                      <  2:581089/141‑1 (MQ=255)
                                                                                                         aCCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGc                                   >  1:531912/1‑141 (MQ=255)
                                                                                                          ccATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCa                                  <  1:51467/141‑1 (MQ=255)
                                                                                                             tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                               >  1:185778/1‑141 (MQ=255)
                                                                                                             tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                               <  1:575656/141‑1 (MQ=255)
                                                                                                             tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                               >  2:191632/1‑141 (MQ=255)
                                                                                                             tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                               >  1:1182/1‑141 (MQ=255)
                                                                                                              cTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACACCAATGACTGAGGccacta                              >  2:482145/1‑136 (MQ=255)
                                                                                                              cTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATa                              <  1:779988/141‑1 (MQ=255)
                                                                                                               ttGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATaa                             <  1:34799/141‑1 (MQ=255)
                                                                                                                  cATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAgg                          <  2:1047332/141‑1 (MQ=255)
                                                                                                                    ttACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTa                        >  1:662725/1‑141 (MQ=255)
                                                                                                                      aCTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATc                      <  1:940324/141‑1 (MQ=255)
                                                                                                                       cTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGcgatacctttatttg                     <  1:807918/141‑15 (MQ=255)
                                                                                                                         aaTGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGcc                   <  1:1067786/141‑1 (MQ=255)
                                                                                                                           tgttgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTc                 >  2:785179/1‑141 (MQ=255)
                                                                                                                            gttgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCa                <  1:652032/141‑1 (MQ=255)
                                                                                                                            gttgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCa                >  2:712040/1‑141 (MQ=255)
                                                                                                                              tgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCAtt              <  1:730267/141‑1 (MQ=255)
                                                                                                                              tgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCAtt              <  1:183996/141‑1 (MQ=255)
                                                                                                                              tgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCAtt              <  1:235402/141‑1 (MQ=255)
                                                                                                                                 aaaTCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTaa           <  1:1380/141‑1 (MQ=255)
                                                                                                                                    tCCTAATGTTGCCAATAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACtt        <  2:431667/141‑1 (MQ=255)
                                                                                                                                     ccTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACttt       <  2:533603/141‑1 (MQ=255)
                                                                                                                                      cTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACtttt      >  1:26752/1‑141 (MQ=255)
                                                                                                                                        aaTGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTa    <  2:681903/141‑1 (MQ=255)
                                                                                                                                          tGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAGa  <  1:79170/141‑1 (MQ=255)
                                                                                                                                          tGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAGa  <  1:323397/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAGA  >  CP000730/2690822‑2691100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: