BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,721,513	A→G	9.2%	N209D (AAT→GAT)	USA300HOU_2575 →	hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,721,513	0	A	G	9.2%	262.6 / 13.7	99	N209D (AAT→GAT)	USA300HOU_2575	hydrolase
Reads supporting (aligned to +/- strand): ref base A (51/38); new base G (3/6); total (55/44)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 105 total aligned reads displayed.

CATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGATATTCCAGG  >  CP000730/2721373‑2721652

cATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaa                                                                                                                                             >  2:616591/1‑141 (MQ=255)

cATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaa                                                                                                                                             >  2:826063/1‑141 (MQ=255)

 aTTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAACCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaaa                                                                                                                                            <  1:39094/141‑1 (MQ=255)

 aTTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCaaa                                                                                                                                            >  1:779737/1‑141 (MQ=255)

  tttGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAat                                                                                                                                           >  2:21451/1‑141 (MQ=255)

   ttGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAata                                                                                                                                          <  2:912212/141‑1 (MQ=255)

    tGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAatat                                                                                                                                         >  1:878320/1‑141 (MQ=255)

     ggtgAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATAtt                                                                                                                                        >  2:706548/4‑141 (MQ=255)

     ggCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATAtt                                                                                                                                        <  2:800633/141‑1 (MQ=255)

     ggCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATAtt                                                                                                                                        >  1:837035/1‑141 (MQ=255)

          aaCATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACttt                                                                                                                                   >  2:657482/1‑141 (MQ=255)

           aCATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTg                                                                                                                                  >  2:844075/1‑141 (MQ=255)

               tAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCATATATTACTTTGgatg                                                                                                                              >  2:378175/1‑141 (MQ=255)

                aaaTATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGgatga                                                                                                                             >  1:838853/1‑141 (MQ=255)

                 aaTATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGgatgat                                                                                                                            >  2:819809/1‑141 (MQ=255)

                 aaTATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGgatgat                                                                                                                            <  2:595421/141‑1 (MQ=255)

                    atCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTc                                                                                                                         >  2:363292/1‑141 (MQ=255)

                     tCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTca                                                                                                                        >  2:752031/1‑141 (MQ=255)

                     tCGCACCAATTGAGGCAAAAATGATGTCTCAACCTGGTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTGca                                                                                                                        <  2:122552/141‑1 (MQ=255)

                      cGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTcac                                                                                                                       <  2:1081226/141‑1 (MQ=255)

                       gCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTcaca                                                                                                                      >  1:59621/1‑141 (MQ=255)

                         aCCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaa                                                                                                                    >  1:304755/1‑141 (MQ=255)

                          ccAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTATGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaaa                                                                                                                   >  2:1015493/1‑141 (MQ=255)

                           cAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAAt                                                                                                                  >  2:783018/1‑141 (MQ=255)

                              ttGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACa                                                                                                               <  1:873715/141‑1 (MQ=255)

                                gATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGc                                                                                                             >  2:869755/1‑141 (MQ=255)

                                 aTGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaaa                                                                                                                   >  1:643313/1‑134 (MQ=255)

                                 aTGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACaaaa                                                                                                                   <  2:643313/134‑1 (MQ=255)

                                  tGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTCCAAACGTACGATGTTCTGGTCAGATTTAGAAATTAGACAATATACGCATTCAGATATTACTTTAGATGATATCACAAAATACAGCGa                                                                                                           >  2:411321/1‑141 (MQ=255)

                                  tGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGa                                                                                                           <  2:75528/141‑1 (MQ=255)

                                    cAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATa                                                                                                         <  2:763634/141‑1 (MQ=255)

                                          tgatgTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCa                                                                                                   <  2:1065958/141‑1 (MQ=255)

                                             tgTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCAccc                                                                                                <  2:555564/141‑1 (MQ=255)

                                               tctcAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCcta                                                                                              <  1:81137/141‑1 (MQ=255)

                                                ctcAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCctac                                                                                             <  1:965079/141‑1 (MQ=255)

                                                   aaCCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTaa                                                                                          >  1:75692/1‑141 (MQ=255)

                                                    aCCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTaaa                                                                                         >  1:771320/1‑141 (MQ=255)

                                                        gctgATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACGAAATggg                                                                                     >  1:1038353/1‑141 (MQ=255)

                                                           gATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGAAAAAAAAAAAAAAATACAGCGATAAAATCACCCTACTAAATGGGACa                                                                                  <  2:593154/141‑1 (MQ=255)

                                                           gATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACa                                                                                  <  2:386567/141‑1 (MQ=255)

                                                            aTACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAg                                                                                 <  2:96335/141‑1 (MQ=255)

                                                               ccgaagagGGACGTATAGAACAGTACAAACGTGCGGCGTTCTTGTTAGAATTCGATATTAGACAATATCCGCACTCCAATAATACTTTGGATGCTTTAACAATATACAGCGATAAAATCACCCTACTAAACGAGAcccagt                                                                              >  2:556513/3‑136 (MQ=255)

                                                                  aagaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTa                                                                           >  2:416878/1‑141 (MQ=255)

                                                                   agaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTag                                                                          >  1:25598/1‑141 (MQ=255)

                                                                   agaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTag                                                                          <  2:125603/141‑1 (MQ=255)

                                                                    gaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTaga                                                                         >  1:309292/1‑141 (MQ=255)

                                                                    gaagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTaga                                                                         >  1:846434/1‑141 (MQ=255)

                                                                     aagGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTagag                                                                        >  1:289087/1‑141 (MQ=255)

                                                                      agGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAgg                                                                       >  1:722942/1‑141 (MQ=255)

                                                                       ggACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGc                                                                      >  1:134109/1‑141 (MQ=255)

                                                                       ggACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGc                                                                      <  1:369191/141‑1 (MQ=255)

                                                                        gACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCt                                                                     <  2:171287/141‑1 (MQ=255)

                                                                         aCGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTc                                                                    >  2:2258/1‑141 (MQ=255)

                                                                          cGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTcc                                                                   >  2:351317/1‑141 (MQ=255)

                                                                          cGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTcc                                                                   <  1:797328/141‑1 (MQ=255)

                                                                             ataGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTc                                                                <  1:675065/141‑1 (MQ=255)

                                                                             ataGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTc                                                                >  2:880354/1‑141 (MQ=255)

                                                                                gAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCca                                                             >  1:243751/1‑141 (MQ=255)

                                                                                gAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCca                                                             <  2:967242/141‑1 (MQ=255)

                                                                                 aaCAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCcac                                                            <  1:646780/141‑1 (MQ=255)

                                                                                  aCAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCcaca                                                           <  2:304755/141‑1 (MQ=255)

                                                                                     gTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGa                                                        <  1:1052132/141‑1 (MQ=255)

                                                                                       aCAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATg                                                      >  2:286770/1‑141 (MQ=255)

                                                                                       aCAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATg                                                      <  2:366879/141‑1 (MQ=255)

                                                                                        cAAACGTACGATGTTCTGGTTATAATTTCAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGTACAGATTCTAGATGCTCCTTCCCACAAGATGt                                                     <  1:1015493/141‑1 (MQ=255)

                                                                                        cAAACGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGt                                                     <  1:476618/141‑1 (MQ=255)

                                                                                          aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                                                   <  1:142275/141‑1 (MQ=255)

                                                                                          aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                                                   >  2:62187/1‑141 (MQ=255)

                                                                                          aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                                                   >  1:14345/1‑141 (MQ=255)

                                                                                          aaCGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTa                                                   >  1:894221/1‑141 (MQ=255)

                                                                                            cGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAAc                                                 >  2:1063641/1‑141 (MQ=255)

                                                                                            cGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAAc                                                 >  2:109403/1‑141 (MQ=255)

                                                                                            cGTACGATGTTCTGGTCAGAATTTGAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCGACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAAc                                                 <  2:968187/141‑1 (MQ=255)

                                                                                             gTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACt                                                >  1:515096/1‑141 (MQ=255)

                                                                                               aCGATGTTCTGGTTAGAGTTTGAAATTCGACAATATACGCATTCAAATATTACTTTGGATGAATTCACAAAATACAGCGATAAAATCACCCTACTATATGGGACAGAATCTAGGGGCTCATTCCCACTAGATGTTAACttt                                              >  2:159364/1‑141 (MQ=255)

                                                                                               aCGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACttt                                              >  1:135728/1‑141 (MQ=255)

                                                                                                  aTGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCAAAAAATACAGCGATAAAATAACCCTACTAAATGGGACAGATTCTAGAGGCTCCGTTCCACCAGATGTTAACTTTtat                                           >  1:327636/1‑141 (MQ=255)

                                                                                                    gTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTtatat                                         >  1:171892/1‑141 (MQ=255)

                                                                                                      tCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATataa                                       <  2:425434/141‑1 (MQ=255)

                                                                                                             aGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGa                                >  1:597748/1‑141 (MQ=255)

                                                                                                                aTTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAAc                             >  2:315950/1‑141 (MQ=255)

                                                                                                                  ttGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTg                           >  2:246848/1‑141 (MQ=255)

                                                                                                                   tGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTgg                          >  1:1076737/1‑141 (MQ=255)

                                                                                                                    gAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGACACTACCCACAACATGTCAACTTCTATATAAATAAAGAAACTGGc                         >  1:762501/1‑141 (MQ=255)

                                                                                                                     aaaTTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCa                        <  1:886008/141‑1 (MQ=255)

                                                                                                                        ttatacacaataCGCAGTCAAATGTTACTTTGGATGATTTCACCAAATACAGCGATAAAACCACCCTACTAATTGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTCAACTTTTATATAAATCAAGAAACTGGCATAc                     <  2:938269/132‑1 (MQ=255)

                                                                                                                        ttAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATAc                     <  1:180816/141‑1 (MQ=255)

                                                                                                                        ttAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATAc                     <  1:920664/141‑1 (MQ=255)

                                                                                                                         tAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATCATATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATAcc                    <  1:1053306/141‑1 (MQ=255)

                                                                                                                          aGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCt                   >  2:516572/1‑141 (MQ=255)

                                                                                                                          aGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCt                   <  2:838853/141‑1 (MQ=255)

                                                                                                                            aCAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTAt                 >  1:704183/1‑141 (MQ=255)

                                                                                                                            aCAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTAt                 <  2:666045/141‑1 (MQ=255)

                                                                                                                             cAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTAtt                >  2:714794/1‑141 (MQ=255)

                                                                                                                                  taCGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGa           <  2:483989/141‑1 (MQ=255)

                                                                                                                                   aCGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGat          <  2:11714/141‑1 (MQ=255)

                                                                                                                                      cATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACACTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGATAtt       <  2:774102/141‑1 (MQ=255)

                                                                                                                                       aTTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGATATTc      >  1:683954/1‑141 (MQ=255)

                                                                                                                                          cAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGATATTCCAg   >  2:954835/1‑141 (MQ=255)

                                                                                                                                           aaaTATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGATATTCCAgg  <  1:21451/141‑1 (MQ=255)

CATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCTGCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTTAGAATTTGAAATTAGACAATATACGCATTCAAATATTACTTTGGATGATTTCACAAAATACAGCGATAAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTTTATATAAATAAAGAAACTGGCATACCTATTGTAGATATTCCAGG  >  CP000730/2721373‑2721652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`