Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,726,571 C→T 5.4% M1M (ATG→ATA) † USA300HOU_2581 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,726,5710CT 5.4% 291.9 / 3.2 93M1M (ATG→ATA) †USA300HOU_2581hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (48/39);  new base T (2/3);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

TATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTATCATT  >  CP000730/2726432‑2726709
                                                                                                                                           |                                                                                                                                          
tATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTCATAGATGATGACGCACTATTGGTCTTAAAAATATCACTTTGAACTTTACtcatca                                                                                                                                           <  1:953477/141‑1 (MQ=255)
tATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACtcatca                                                                                                                                           <  1:204226/141‑1 (MQ=255)
 aTGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTcatcat                                                                                                                                          >  1:516322/1‑141 (MQ=255)
  tGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCAtt                                                                                                                                         >  2:171934/1‑141 (MQ=255)
   gAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCAttt                                                                                                                                        >  2:317760/1‑141 (MQ=255)
     gCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTac                                                                                                                                      >  2:432795/1‑141 (MQ=255)
      cATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTaca                                                                                                                                     <  2:259587/141‑1 (MQ=255)
        ttattattTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACAcc                                                                                                                                   <  1:925282/141‑1 (MQ=255)
         tattattTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACct                                                                                                                                  >  2:94047/1‑141 (MQ=255)
             attTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACctctct                                                                                                                              <  1:92684/141‑1 (MQ=255)
             attTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACctctct                                                                                                                              <  1:96501/141‑1 (MQ=255)
                   aCTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCAttt                                                                                                                        <  2:185015/141‑1 (MQ=255)
                    cTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTa                                                                                                                       >  2:423088/1‑141 (MQ=255)
                     ttACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTaa                                                                                                                      >  2:948496/1‑141 (MQ=255)
                      tACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGACCTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTaaa                                                                                                                     <  1:270291/141‑1 (MQ=255)
                         tgttgtACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATTGATTTTGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAatta                                                                                                                  >  2:433006/1‑141 (MQ=255)
                          gttgtACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAattat                                                                                                                 <  1:424631/141‑1 (MQ=255)
                           ttgtACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAattatt                                                                                                                <  1:931270/141‑1 (MQ=255)
                           ttgtACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAattatt                                                                                                                >  2:293963/1‑141 (MQ=255)
                             gtACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTAtttt                                                                                                              >  2:244932/1‑141 (MQ=255)
                             gtACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTAtttt                                                                                                              <  1:205588/141‑1 (MQ=255)
                              tACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTa                                                                                                             <  1:54395/141‑1 (MQ=255)
                                  ttCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAat                                                                                                         >  1:520616/1‑141 (MQ=255)
                                  ttCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAat                                                                                                         <  1:479527/141‑1 (MQ=255)
                                    cATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACGGCACTTTTAATAGATGATGATGCACTATTAGTCTTGAAAATATCACTTTGAACTGTACTCATTATTTACACCTCTTCCCATTTAAATTATTTTAAAatta                                                                                                       <  2:153674/141‑1 (MQ=255)
                                    cATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAatta                                                                                                       >  2:21149/1‑141 (MQ=255)
                                     aTCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAattat                                                                                                      <  2:434397/141‑1 (MQ=255)
                                     aTCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAattat                                                                                                      <  2:434357/141‑1 (MQ=255)
                                        tttATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAg                                                                                                   <  1:896349/141‑1 (MQ=255)
                                          tATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAAAAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGaa                                                                                                 >  2:1050067/1‑141 (MQ=255)
                                            tCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAAc                                                                                               <  2:521327/141‑1 (MQ=255)
                                               ggTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGt                                                                                            >  2:203655/1‑141 (MQ=255)
                                                gTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTa                                                                                           <  2:279056/141‑1 (MQ=255)
                                                gTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTa                                                                                           <  2:942921/141‑1 (MQ=255)
                                                gTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTa                                                                                           >  2:917189/1‑141 (MQ=255)
                                                 tttCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTAt                                                                                          <  2:878411/141‑1 (MQ=255)
                                                   tCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCa                                                                                        >  2:994410/1‑141 (MQ=255)
                                                      acacATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAata                                                                                     <  1:428333/141‑1 (MQ=255)
                                                       cacaTTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTa                                                                                           >  1:320141/1‑134 (MQ=255)
                                                       cacaTTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTa                                                                                           <  2:320141/134‑1 (MQ=255)
                                                       cacaTTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAatat                                                                                    >  1:72195/1‑141 (MQ=255)
                                                       cacaTTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAatat                                                                                    <  1:254762/141‑1 (MQ=255)
                                                        acaTTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAatata                                                                                   <  1:672608/141‑1 (MQ=255)
                                                          attattACATGTTTCAACGGCACTTTTAATAGATGATGATGCACTATTAGTCTTGAAAATATCACTTTGAACTGTACTCATTATTTACACCTCTTCCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATg                                                                                 <  1:212378/141‑1 (MQ=255)
                                                            tattaCATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAatatcatat                                                                               <  1:509892/141‑6 (MQ=255)
                                                              ttaCATGTTTCAACAGCGCTTTTAATAGATGATGATGCGCTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGatat                                                                             >  2:28179/1‑141 (MQ=255)
                                                              ttaCATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGatat                                                                             >  2:513331/1‑141 (MQ=255)
                                                                  aTGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCaa                                                                         <  1:956473/141‑1 (MQ=255)
                                                                   tGTTTCAACGGCACTTTTAATAGATGATGATGCACTATTAGTCTTGAAAATATCACTTTGAACTGTACTCATTATTTACACCTCTTCCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCaaa                                                                        >  2:964946/1‑141 (MQ=255)
                                                                   tGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCaaa                                                                        >  1:585444/1‑141 (MQ=255)
                                                                   tGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCaaa                                                                        >  2:966708/1‑141 (MQ=255)
                                                                       tCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCAATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACAtt                                                                    >  1:701880/1‑141 (MQ=255)
                                                                            aGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAAtt                                                               >  1:180074/1‑141 (MQ=255)
                                                                                 ttttAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTCAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACt                                                          >  2:947550/1‑141 (MQ=255)
                                                                                 ttttAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCACCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACt                                                          <  2:25504/141‑1 (MQ=255)
                                                                                 ttttAATAGATGATGATGCACTATTAGTCTTGAAAATATCACTTTGAACTGTACTCTTTATTTACACCTCTTCCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATAATACt                                                          <  1:884672/141‑1 (MQ=255)
                                                                                   ttAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTa                                                        <  1:124957/141‑1 (MQ=255)
                                                                                   ttAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTa                                                        <  1:773080/141‑1 (MQ=255)
                                                                                   ttAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAAAATTCCATtatattgt                                                        >  2:39928/1‑137 (MQ=255)
                                                                                   ttAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATAATTCTTTCCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTa                                                        <  1:293963/141‑1 (MQ=255)
                                                                                    tAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTaa                                                       >  2:954442/1‑141 (MQ=255)
                                                                                     aaTAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTaaa                                                      <  2:1012577/141‑1 (MQ=255)
                                                                                     aaTAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTAACAATCTATTATATTCAAACATTCAATTATACTTaaa                                                      <  1:252926/141‑1 (MQ=255)
                                                                                      aTAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAat                                                     >  1:807245/1‑141 (MQ=255)
                                                                                      aTAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAat                                                     >  2:52868/1‑141 (MQ=255)
                                                                                      aTAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTAATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAat                                                     <  2:686392/141‑1 (MQ=255)
                                                                                        aGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAatat                                                   <  2:433997/141‑1 (MQ=255)
                                                                                          atgatgatgCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTg                                                 >  2:607622/1‑141 (MQ=255)
                                                                                           tgatgatgCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATCCTTAAATATTGt                                                >  2:157874/1‑141 (MQ=255)
                                                                                           tgatgatgCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGt                                                >  1:194638/1‑141 (MQ=255)
                                                                                              tgatgCCCTATAGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTATCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCCATATATGATCTTCAACCATTCAATCATACTAAAATATAGtatt                                             >  2:1078735/1‑141 (MQ=255)
                                                                                                atgCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTAt                                           >  2:609074/1‑141 (MQ=255)
                                                                                                 tgCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATg                                          >  2:958644/1‑141 (MQ=255)
                                                                                                 tgCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATg                                          >  2:66604/1‑141 (MQ=255)
                                                                                                     cTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATta                                      >  1:344346/1‑141 (MQ=255)
                                                                                                       aTTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATtata                                    >  1:217342/1‑141 (MQ=255)
                                                                                                         tGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATAtt                                  <  2:846956/141‑1 (MQ=255)
                                                                                                          ggTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTc                                 <  1:949644/141‑1 (MQ=255)
                                                                                                            tCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCaa                               <  2:1082080/141‑1 (MQ=255)
                                                                                                              ttAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCaaaa                             <  1:625996/141‑1 (MQ=255)
                                                                                                               tAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAat                            >  1:294412/1‑141 (MQ=255)
                                                                                                                aaaaaTATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAatt                           <  1:977941/141‑1 (MQ=255)
                                                                                                                 aaaaTATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAatta                          >  1:46011/1‑141 (MQ=255)
                                                                                                                  aaaTATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAattat                         <  2:600043/141‑1 (MQ=255)
                                                                                                                    atatCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTg                       >  1:21780/1‑141 (MQ=255)
                                                                                                                    ataaCACTTTGAACTGTACTCATCATCTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCTATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTg                       <  2:971898/141‑1 (MQ=255)
                                                                                                                       tCACTTTGAACTGTACTCATTATTTACACCTCTTCCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATAATACTTAAATATTGTTTTGTGATTATATTCAAAATTATTGTAt                    >  2:680371/1‑141 (MQ=255)
                                                                                                                       tCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTAt                    >  2:893711/1‑141 (MQ=255)
                                                                                                                       tCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTAt                    >  2:796014/1‑141 (MQ=255)
                                                                                                                        cACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATc                   <  2:187259/141‑1 (MQ=255)
                                                                                                                            ttGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCaata               >  2:322068/1‑141 (MQ=255)
                                                                                                                                aCTGTACTCATCATTTACACCTCTCTCCATTTACATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCaataaatt           >  2:480838/1‑140 (MQ=255)
                                                                                                                                 cTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAatat          >  1:796810/1‑141 (MQ=255)
                                                                                                                                    tACTCATCATTTACACCTCTCTCCCTTTAAAATATTTGGAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTa       >  1:575537/1‑141 (MQ=255)
                                                                                                                                    tACTCATCATTTACACCTCTCTCCAGTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTa       >  2:57444/1‑141 (MQ=255)
                                                                                                                                      cTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTATc     >  2:999730/1‑141 (MQ=255)
                                                                                                                                       tcatcaTTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTATCa    <  1:941220/141‑1 (MQ=255)
                                                                                                                                         atcatTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGAAATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTATCAtt  <  2:516322/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGATGATGCACTATTGGTCTTAAAAATATCACTTTGAACTGTACTCATCATTTACACCTCTCTCCATTTAAATTATTTTAAAATTATTAGAAACTGTATCAATATATGATATTCAAACATTCAATTATACTTAAATATTGTTTTATGATTATATTCAAAATTATTGTATCAATAAATATGTATCATT  >  CP000730/2726432‑2726709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: