Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,797,788 T→C 5.6% intergenic (+76/+191) acmB → / ← USA300HOU_2645 possible N‑acetylmuramoyl‑L‑alanine amidase/possible isochorismatase hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,797,7880TC 5.6% 306.7 / 5.4 105intergenic (+76/+191)acmB/USA300HOU_2645possible N‑acetylmuramoyl‑L‑alanine amidase/possible isochorismatase hydrolase
Reads supporting (aligned to +/- strand):  ref base T (51/48);  new base C (2/4);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

ATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATAAATATGAGCAA  >  CP000730/2797650‑2797927
                                                                                                                                          |                                                                                                                                           
aTTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGa                                                                                                                                           >  2:594478/1‑141 (MQ=255)
   tctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACTGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGAttt                                                                                                                                        <  2:685109/141‑1 (MQ=255)
   tctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGAttt                                                                                                                                        <  2:227442/141‑1 (MQ=255)
    ctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTc                                                                                                                                       >  2:904272/1‑141 (MQ=255)
      cATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCtt                                                                                                                                     >  2:579474/1‑141 (MQ=255)
        tAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATGAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAt                                                                                                                                   <  2:124595/141‑1 (MQ=255)
        tAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAt                                                                                                                                   <  1:141695/141‑1 (MQ=255)
         aGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAtt                                                                                                                                  >  2:957835/1‑141 (MQ=255)
         aGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAtt                                                                                                                                  >  1:311401/1‑141 (MQ=255)
         aGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCCTAtt                                                                                                                                  >  2:560023/1‑141 (MQ=255)
          gAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTc                                                                                                                                 >  1:1082785/1‑141 (MQ=255)
              tATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAAtt                                                                                                                             <  2:910010/141‑1 (MQ=255)
                tCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTGTAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaa                                                                                                                           >  2:294387/1‑141 (MQ=255)
                 cAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaaa                                                                                                                          <  2:379573/141‑1 (MQ=255)
                 cAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaaa                                                                                                                          >  2:444094/1‑141 (MQ=255)
                 cAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaaa                                                                                                                          >  2:35904/1‑141 (MQ=255)
                  aaCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAc                                                                                                                         >  1:930865/1‑141 (MQ=255)
                   aCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAcc                                                                                                                        >  2:495835/1‑141 (MQ=255)
                   aCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAcc                                                                                                                        <  1:148916/141‑1 (MQ=255)
                   aCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTAATAAGTCTGATTTCTTATTCAATTAAAcc                                                                                                                        <  1:472895/141‑1 (MQ=255)
                    cGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAccc                                                                                                                       >  2:722438/1‑141 (MQ=255)
                    cGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAccc                                                                                                                       <  1:673431/141‑1 (MQ=255)
                       agcggcagCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAg                                                                                                                    >  2:515319/8‑141 (MQ=255)
                        gCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAGCCCGAgg                                                                                                                   <  2:991487/141‑1 (MQ=255)
                        gCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAgg                                                                                                                   <  1:518453/141‑1 (MQ=255)
                          tGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGTAtt                                                                                                               >  1:835417/1‑141 (MQ=39)
                           gCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCAt                                                                                                                >  2:1026604/1‑141 (MQ=255)
                            ccGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCAtt                                                                                                               >  2:477989/1‑141 (MQ=255)
                             cGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTc                                                                                                              <  1:286579/141‑1 (MQ=255)
                             cGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTc                                                                                                              >  2:461619/1‑141 (MQ=255)
                                 gaagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGtt                                                                                                          >  1:1018698/1‑141 (MQ=255)
                                 gaagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGtt                                                                                                          >  1:1018120/1‑141 (MQ=255)
                                  aagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTc                                                                                                         <  2:930281/141‑1 (MQ=255)
                                  aagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTc                                                                                                         <  2:871908/141‑1 (MQ=255)
                                  aagaaTTATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactga                                                                                                       <  1:623925/141‑8 (MQ=255)
                                    gaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactga                                                                                                       <  2:971120/141‑8 (MQ=255)
                                    gaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactga                                                                                                       <  1:1048199/141‑8 (MQ=255)
                                    gaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGa                                                                                                       <  2:978982/141‑1 (MQ=255)
                                     aaTTATCATATATTACAGGTAAATAAGTATTATATTAGACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGaa                                                                                                      >  1:783070/1‑141 (MQ=255)
                                      aTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactgaat                                                                                                     <  1:673422/141‑10 (MQ=255)
                                       ttATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGTATTCAGTTCGAACGcc                                                                                                  <  2:1060832/141‑1 (MQ=39)
                                        tATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTAAAAGTATAAACAAGGAGTTCGGTCTTAAACATATTTCTGTTCATAAGTCCGAGTTCTTATTCAATTAAACCCGAGGCGTTcgaactgaatacct                                                                                                 <  1:718075/141‑14 (MQ=255)
                                         aTCATATATCACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGcc                                                                                                  >  1:953284/1‑141 (MQ=255)
                                              atatTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCggg                                                                                             <  1:1005938/141‑1 (MQ=255)
                                              atatTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCggg                                                                                             >  2:459866/1‑141 (MQ=255)
                                              atatTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACACGAGGCATTCAGTTCGAACGCCTCggg                                                                                             >  2:1057006/1‑141 (MQ=255)
                                                     aGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGtttt                                                                                      >  1:148743/1‑141 (MQ=255)
                                                     aGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGtttt                                                                                      >  1:379595/1‑141 (MQ=255)
                                                         aaaTAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTtatat                                                                                  >  1:745337/1‑141 (MQ=255)
                                                         aaaTAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTtatat                                                                                  >  2:826793/1‑141 (MQ=255)
                                                          aaTAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATATGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGTGTCGTTTtatata                                                                                 >  1:758663/1‑141 (MQ=255)
                                                              aGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAata                                                                             >  1:622519/1‑141 (MQ=255)
                                                                tattatattaAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAatata                                                                           >  2:385297/1‑141 (MQ=255)
                                                                    atattaAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATAtattat                                                                       <  2:287517/141‑1 (MQ=255)
                                                                      attaAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTAttt                                                                     >  1:761767/1‑141 (MQ=255)
                                                                      attaAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTAttt                                                                     >  1:589994/1‑141 (MQ=255)
                                                                        taAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTa                                                                   <  2:632181/141‑1 (MQ=255)
                                                                        taAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTa                                                                   <  2:735825/141‑1 (MQ=255)
                                                                         aaaCCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTAt                                                                  >  2:1086024/1‑141 (MQ=255)
                                                                           aCCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGTATTCAGTTCGAACGCCTCGGGTCATTTTATATAAATATATTATTTTATGt                                                                <  1:354592/141‑1 (MQ=255)
                                                                            cccGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGtt                                                               <  2:904993/141‑1 (MQ=255)
                                                                            cccGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGtt                                                               <  2:795384/141‑1 (MQ=255)
                                                                              cGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCa                                                             <  1:949658/141‑1 (MQ=255)
                                                                                tAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCaaa                                                           >  1:615312/1‑141 (MQ=255)
                                                                                tAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCaaa                                                           <  2:912822/141‑1 (MQ=255)
                                                                                  aaaTTTATAAGTATAAACAAGGAGTTCGGACGTAAACACATTTCTGATCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATg                                                         >  2:48755/1‑141 (MQ=255)
                                                                                   aaTTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGt                                                        <  2:398927/141‑1 (MQ=255)
                                                                                   aaTTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGt                                                        >  2:1014439/1‑141 (MQ=255)
                                                                                    aTTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGtt                                                       >  2:326617/1‑141 (MQ=255)
                                                                                     tttATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTc                                                      >  2:989734/1‑141 (MQ=255)
                                                                                       tgtaAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCCTTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCt                                                    <  1:294387/139‑1 (MQ=255)
                                                                                         taAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCGATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAACCATATCTTTTTATGTTCAAATGTTCCtca                                                  >  1:505252/1‑141 (MQ=255)
                                                                                          aaGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCtcat                                                 <  2:661736/141‑1 (MQ=255)
                                                                                            gTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCtcatca                                               <  1:940179/141‑1 (MQ=255)
                                                                                            gTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCtcatca                                               <  2:410807/141‑1 (MQ=255)
                                                                                             tataAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGt                                                                                                           >  2:178876/1‑80 (MQ=255)
                                                                                             tataAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGt                                                                                                           <  1:178876/80‑1 (MQ=255)
                                                                                                 aaCAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATcc                                          <  2:842284/141‑1 (MQ=255)
                                                                                                   cAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGt                                        <  1:538960/141‑1 (MQ=255)
                                                                                                    aaGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGtt                                       <  1:1016047/141‑1 (MQ=255)
                                                                                                         gTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAAt                                  >  2:84532/1‑141 (MQ=255)
                                                                                                             ggACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGcc                              >  1:203038/1‑141 (MQ=255)
                                                                                                              gACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCa                             >  2:1056568/1‑141 (MQ=255)
                                                                                                               aCTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATTTTCCTCATCATATACGTTTCAATTGCCAt                            >  2:570449/1‑141 (MQ=255)
                                                                                                                     aCATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTcaca                      >  2:421453/1‑141 (MQ=255)
                                                                                                                     aCATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTcaca                      >  2:551249/1‑141 (MQ=255)
                                                                                                                     aCATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTcaca                      <  1:336396/141‑1 (MQ=255)
                                                                                                                      cATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTcacac                     <  1:996020/141‑1 (MQ=255)
                                                                                                                      cATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTcacac                     <  2:939755/141‑1 (MQ=255)
                                                                                                                          tgtCTGTTCATAAGTCTGATTTCTTATTCAATTAAGCCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATAGTATTTCATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACAttt                 <  2:612386/139‑1 (MQ=255)
                                                                                                                           ttCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACAtttt                <  2:34906/141‑1 (MQ=255)
                                                                                                                           ttCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACAtttt                <  2:35180/141‑1 (MQ=255)
                                                                                                                             cTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTtat              <  1:716330/141‑1 (MQ=255)
                                                                                                                                ttCATAGGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATaaa           >  2:847048/1‑141 (MQ=255)
                                                                                                                                ttCATAAGTCTGATTTCTTATCCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTtataca           >  2:323190/1‑139 (MQ=255)
                                                                                                                                      aGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCTGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATAAATATGAg     <  1:157380/141‑1 (MQ=255)
                                                                                                                                       gTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATAAATATGAGc    <  2:696669/141‑1 (MQ=255)
                                                                                                                                        tCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATAAATATGAGCa   <  1:817769/141‑1 (MQ=255)
                                                                                                                                         cTGATTTCTTATTCATTTAAACCCTAGTCACTCAGTCCGAACGCCTCGGACCGTTTTATATAAATATATTATTTTAGGTTCAAATGTTCCGCATCATATTCGTTTCAATTGCCATCTCACACATTTTATCAATATGAGCaa  >  2:106741/1‑141 (MQ=255)
                                                                                                                                         cTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATAAATATGAGCaa  >  2:586597/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTATTTTATGTTCAAATGTTCCTCATCATATCCGTTTCAATTGCCATCTCACACATTTTATAAATATGAGCAA  >  CP000730/2797650‑2797927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: