Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 181,798 T→G 6.5% G164G (GGT→GGG cap5H → capsular polysaccharide biosynthesis protein Cap5H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730181,7980TG 6.5% 221.2 / 6.4 76G164G (GGT→GGGcap5Hcapsular polysaccharide biosynthesis protein Cap5H
Reads supporting (aligned to +/- strand):  ref base T (36/35);  new base G (2/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

GCCGTACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAAAAAGGATACTTAATGAT  >  CP000730/181659‑181937
                                                                                                                                           |                                                                                                                                           
gCCGTACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGgtggtg                                                                                                                                            >  2:612565/1‑141 (MQ=255)
  cGTACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGtt                                                                                                                                          <  2:78176/141‑1 (MQ=255)
  cGTACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGATTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGGGtt                                                                                                                                          <  2:409042/141‑1 (MQ=255)
   gTACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTc                                                                                                                                         >  2:660270/1‑141 (MQ=255)
    tACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTcc                                                                                                                                        <  2:128574/141‑1 (MQ=255)
     acaacaATTAAAAATGATGTGTGGATTGGTGCAAATGTAGGTATTATGGAGGGGTTAACAAGAGAAACTGCTGCAGTCATTGCAGGCGGCGCGGTTTTTTCGAAAAATGTAGTAGCATATGAGGTTGTTGGGGGTGGTccg                                                                                                                                       >  1:542284/1‑140 (MQ=255)
     acaacaATTAAAAATGATGTGTGGATTGGTGAAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCt                                                                                                                                       <  1:834196/141‑1 (MQ=255)
      caacaaTTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTg                                                                                                                                      <  1:699498/141‑1 (MQ=255)
             tAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGt                                                                                                                               <  2:734859/141‑1 (MQ=255)
                aaaTGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGAt                                                                                                                            >  2:259826/1‑141 (MQ=255)
                aaaTGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGAATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGAt                                                                                                                            >  2:1021294/1‑141 (MQ=255)
                 aaTGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGAtt                                                                                                                           <  1:1059732/141‑1 (MQ=255)
                 aaTGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGAtt                                                                                                                           <  1:936980/141‑1 (MQ=255)
                    gatgTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTaag                                                                                                                        <  2:212354/141‑1 (MQ=255)
                     atgTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTaaga                                                                                                                       >  2:882069/1‑141 (MQ=255)
                     atgTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTaaga                                                                                                                       >  2:540238/1‑141 (MQ=255)
                      tgtgtgGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTaagaa                                                                                                                      <  2:485005/141‑1 (MQ=255)
                      tgtgtgGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTaagaa                                                                                                                      <  1:344924/141‑1 (MQ=255)
                      tgtgtgGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTaagaa                                                                                                                      >  2:421182/1‑141 (MQ=255)
                        tgtgGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGc                                                                                                                    >  1:773091/1‑141 (MQ=255)
                        tgtgGATTGGTGCAAATGTAATTATTATGGATGGATTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGGGTTCCTGCAAAAGTGATTAAGAAGc                                                                                                                    <  1:180991/141‑1 (MQ=255)
                         gtgGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCAAATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCg                                                                                                                   >  1:10435/1‑141 (MQ=255)
                             aTTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGAttt                                                                                                               <  2:984514/141‑1 (MQ=255)
                              ttGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTg                                                                                                              <  2:468224/141‑1 (MQ=255)
                                ggTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGAc                                                                                                            >  2:1073443/1‑141 (MQ=255)
                                     aaaTGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGCTTAAGAAGCGATTTGACAATaa                                                                                                       >  2:859940/1‑141 (MQ=255)
                                     aaaTGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATaa                                                                                                       >  1:597957/1‑141 (MQ=255)
                                         gTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACa                                                                                                   <  1:1015900/141‑1 (MQ=255)
                                              tattatGGATGGTTTAACCATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTACCAGTGTTGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGa                                                                                              <  1:376760/141‑1 (MQ=255)
                                              tattatGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGa                                                                                              <  1:434200/141‑1 (MQ=255)
                                              tattatGGATGGATTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGGGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGa                                                                                              <  1:90792/141‑1 (MQ=255)
                                                           ttAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGaa                                                                                 <  1:950850/141‑1 (MQ=255)
                                                               cAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCa                                                                             >  2:781509/1‑141 (MQ=255)
                                                               cAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCa                                                                             <  1:797897/141‑1 (MQ=255)
                                                                aataaataCTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCaa                                                                            <  1:656834/141‑1 (MQ=255)
                                                                    aataCTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAgtgg                                                                        >  1:850431/1‑141 (MQ=255)
                                                                    aataCTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTACCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAgtgg                                                                        <  1:294215/141‑1 (MQ=255)
                                                                        cTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTggg                                                                    <  2:290676/141‑1 (MQ=255)
                                                                        cTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTggg                                                                    <  1:178707/141‑1 (MQ=255)
                                                                        cTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGGGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTggg                                                                    >  1:564828/1‑141 (MQ=255)
                                                                        cTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGGGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTggg                                                                    >  1:564927/1‑141 (MQ=255)
                                                                         tGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGga                                                                   >  2:117289/1‑141 (MQ=255)
                                                                            tGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTCGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGaa                                                                <  1:801197/141‑1 (MQ=255)
                                                                             gCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGAGATTAAGAAGCGATTTCACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGaaa                                                               <  1:1030439/141‑1 (MQ=255)
                                                                                gTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACg                                                            <  2:435492/141‑1 (MQ=255)
                                                                                gTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACg                                                            >  2:239930/1‑141 (MQ=255)
                                                                                 tCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGc                                                           <  2:73746/141‑1 (MQ=255)
                                                                                 tCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGc                                                           <  1:170043/141‑1 (MQ=255)
                                                                                  cATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGcc                                                          <  2:617430/141‑1 (MQ=255)
                                                                                    tAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTg                                                        >  1:958352/1‑141 (MQ=255)
                                                                                      gcagcCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGAc                                                      <  1:941585/141‑1 (MQ=255)
                                                                                        agcCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACaa                                                    >  2:432816/1‑141 (MQ=255)
                                                                                        agcCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACaa                                                    >  1:67119/1‑141 (MQ=255)
                                                                                         gcCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACaaa                                                   >  2:160845/1‑141 (MQ=255)
                                                                                          ccGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAAc                                                  >  1:564397/1‑141 (MQ=255)
                                                                                            ggCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTa                                                >  2:17987/1‑141 (MQ=255)
                                                                                             gCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTaa                                               >  1:479545/1‑141 (MQ=255)
                                                                                             gCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTaa                                               >  1:563600/1‑141 (MQ=255)
                                                                                              cTCAGTTGTTGCTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTaaa                                              <  2:940283/141‑1 (MQ=255)
                                                                                               tCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCa                                                                                                                                    >  2:108169/1‑54 (MQ=255)
                                                                                               tCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCa                                                                                                                                    <  1:108169/54‑1 (MQ=255)
                                                                                                 aGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAgg                                           >  1:1064916/1‑141 (MQ=255)
                                                                                                   ttgttACTAACAATGTAGGAGAATCTGCGGTTGTTGGTGGTGTTCCTGCAAACGTGCTTAAGAAGAGATTTGACAATACAACACTTGACAAACTTTTGGCACGCAAGTGGTGGGAGAAAACGCCTGACACACTAACGGGAt                                         <  2:542284/141‑1 (MQ=255)
                                                                                                     gttACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGAttt                                       <  2:272305/141‑1 (MQ=255)
                                                                                                             aaaTGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGa                               >  2:61262/1‑141 (MQ=255)
                                                                                                              aaTGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGaa                              >  1:1071794/1‑141 (MQ=255)
                                                                                                               aTGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAat                             >  1:333410/1‑141 (MQ=255)
                                                                                                                 gTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAatat                           >  1:64657/1‑141 (MQ=255)
                                                                                                                  tAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATAtt                          >  1:935618/1‑141 (MQ=255)
                                                                                                                   aGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATAttt                         >  1:641539/1‑141 (MQ=255)
                                                                                                                   aGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATAttt                         >  1:870428/1‑141 (MQ=255)
                                                                                                                    ggAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTta                        <  1:794109/141‑1 (MQ=255)
                                                                                                                     gAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAGTATTtaa                       <  2:638995/141‑1 (MQ=255)
                                                                                                                     gAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTtaa                       >  2:563946/1‑141 (MQ=255)
                                                                                                                            tGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATaaaaa                >  2:105981/1‑141 (MQ=255)
                                                                                                                            tGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATaaaaa                <  2:392839/141‑1 (MQ=255)
                                                                                                                                    gtgggggTGATCCTGCAAAGGAGAGTAAGGGGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAACAAGGATACtt        >  2:133144/2‑141 (MQ=255)
                                                                                                                                        tggtgTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAAAAAGGATACTTAATg    >  2:188694/1‑141 (MQ=255)
                                                                                                                                         ggtgTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAAAAAGGATACTTAATGa   >  1:679380/1‑141 (MQ=255)
                                                                                                                                         ggtgTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATGTAAATAAAAAGGATACTTAATGa   >  2:705343/1‑141 (MQ=255)
                                                                                                                                          gtgtTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAAAAAGGATACTTAATGat  <  2:187043/141‑1 (MQ=255)
                                                                                                                                          gtgtTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAAAAAGGATACTTAATGat  >  2:610723/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCCGTACAACAATTAAAAATGATGTGTGGATTGGTGCAAATGTAATTATTATGGATGGTTTAACAATAAATACTGGTGCAGTCATAGCAGCCGGCTCAGTTGTTACTAAAAATGTAGGAGCATATGAGGTTGTTGGTGGTGTTCCTGCAAAAGTGATTAAGAAGCGATTTGACAATAAAACAATTGAAAAACTTTTGGAAAGCAAGTGGTGGGAGAAAACGCCTGACAAACTAAAAGGATTTTCGGTTGAATATTTAAATAAAAAGGATACTTAATGAT  >  CP000730/181659‑181937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: