Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 182,889 C→T 7.8% L318L (CTA→TTA)  cap5I → capsular polysaccharide biosynthesis protein Cap5I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730182,8890CT 7.8% 280.4 / 7.9 96L318L (CTA→TTA) cap5Icapsular polysaccharide biosynthesis protein Cap5I
Reads supporting (aligned to +/- strand):  ref base C (41/47);  new base T (5/3);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTTAG  >  CP000730/182750‑183027
                                                                                                                                           |                                                                                                                                          
gACGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCt                                                                                                                                           >  1:979823/1‑141 (MQ=255)
 aCGAAATCATTATTTATTCATTTTGAATATACAGTATCTAATAAAATTTTTGCATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTa                                                                                                                                          <  2:558629/141‑1 (MQ=255)
 aCGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTa                                                                                                                                          <  1:838280/141‑1 (MQ=255)
  cGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTaa                                                                                                                                         >  2:564055/1‑141 (MQ=255)
  cGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTaa                                                                                                                                         >  2:235867/1‑141 (MQ=255)
    aaaCCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAg                                                                                                                                       <  2:191729/141‑1 (MQ=255)
       ccAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGaa                                                                                                                                    >  2:446940/1‑141 (MQ=255)
        cAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAg                                                                                                                                   >  2:1033805/1‑141 (MQ=255)
           tATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTa                                                                                                                                <  1:989207/141‑1 (MQ=255)
                aTTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCg                                                                                                                           <  1:1078212/141‑1 (MQ=255)
                 ttAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGt                                                                                                                          >  1:557922/1‑141 (MQ=255)
                 ttAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGt                                                                                                                          >  1:810148/1‑141 (MQ=255)
                 ttAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGt                                                                                                                          >  1:985759/1‑141 (MQ=255)
                        tGAATATACAGTATCTAATAAAATTTTTTTTTTTTTTTTTGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAAt                                                                                                                   >  2:497371/1‑141 (MQ=255)
                         gAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAAtt                                                                                                                  >  1:601351/1‑141 (MQ=255)
                             ataCAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGaaa                                                                                                              >  1:519031/1‑141 (MQ=255)
                                cAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAgg                                                                                                           >  1:296628/1‑141 (MQ=255)
                                cAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAgg                                                                                                           >  1:296035/1‑141 (MQ=255)
                                 aGTATCTAATATAATTTTTGAATGTATACATGCTTGTTTACTAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTTAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGc                                                                                                          <  2:873063/141‑1 (MQ=255)
                                 aGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGc                                                                                                          <  2:424914/141‑1 (MQ=255)
                                    atataataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTAAAAAAAAAAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGt                                                                                                       <  2:676651/138‑1 (MQ=255)
                                    aTCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGt                                                                                                       <  2:726995/141‑1 (MQ=255)
                                      cTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTa                                                                                                     <  1:987712/141‑1 (MQ=255)
                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:605946/141‑1 (MQ=255)
                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:931321/141‑1 (MQ=255)
                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:1007875/141‑1 (MQ=255)
                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:484833/141‑1 (MQ=255)
                                       taataaAATTTTTGAATATATACATGCTTGTTTAACAGTAATTTTATCTCCTGTCAAAGAGTATATTTATCTCAATGAAAAATATAAATTTGGCATTGTGCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:630127/141‑1 (MQ=255)
                                        aataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGa                                                                                                   <  1:81554/141‑1 (MQ=255)
                                        aataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTAAAATAAATAAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGa                                                                                                   <  2:969118/141‑1 (MQ=255)
                                        aataaAATTCTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGa                                                                                                   >  1:955395/1‑141 (MQ=255)
                                         ataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGaa                                                                                                  <  1:864385/141‑1 (MQ=255)
                                         ataaAATTTTTGAATGTATACATGCTGGGTTACCAGTAATTTTATCTCCTGTTAAAGAGCATATTTATCTCAATGAAAAATATCAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAAAAATTAAAAAGTCGGTTAGaa                                                                                                  >  2:431513/1‑141 (MQ=255)
                                           aaaaTTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGaaaa                                                                                                <  2:663544/141‑1 (MQ=255)
                                                ttttGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAag                                                                                           >  1:558220/1‑141 (MQ=255)
                                                 tttGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAaga                                                                                          >  1:374652/1‑141 (MQ=255)
                                                   tGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAgaga                                                                                        >  2:610575/1‑141 (MQ=255)
                                                   tGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAgaga                                                                                        <  2:253201/141‑1 (MQ=255)
                                                     caTGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCAGTGTTCTAAGGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAACATTCAGAGATa                                                                                      >  2:754073/2‑141 (MQ=255)
                                                      aTGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATaa                                                                                     >  1:653362/1‑141 (MQ=255)
                                                        gTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATc                                                                                   <  1:135992/141‑1 (MQ=255)
                                                         tataCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCa                                                                                  <  1:435444/141‑1 (MQ=255)
                                                           taCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACg                                                                                <  2:954286/141‑1 (MQ=255)
                                                           taCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACg                                                                                >  1:233123/1‑141 (MQ=255)
                                                            aCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGa                                                                               <  2:557922/141‑1 (MQ=255)
                                                              aTGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGAtt                                                                             <  2:570136/141‑1 (MQ=255)
                                                                gCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTg                                                                           >  1:754598/1‑141 (MQ=255)
                                                                gCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATAAAGAGATAATCACGATTTg                                                                           >  1:554919/1‑141 (MQ=255)
                                                                   ggTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGttt                                                                        <  1:545610/141‑1 (MQ=255)
                                                                   ggTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGttt                                                                        <  1:170137/141‑1 (MQ=255)
                                                                    gTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTa                                                                       >  2:870187/1‑141 (MQ=255)
                                                                     tttACCAGTAATTTTCCCTCGTGTCAAAGAGCATATTTATCTCAATGAAAAAGATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTaa                                                                      <  1:332687/141‑1 (MQ=255)
                                                                          cAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCAtt                                                                 <  1:150739/141‑1 (MQ=255)
                                                                              aaTTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTgatccaatcgctttg                                                             >  1:805172/1‑127 (MQ=255)
                                                                               aTTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGt                                                            <  1:249517/141‑1 (MQ=255)
                                                                                 tttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCa                                                          >  1:251443/1‑141 (MQ=255)
                                                                                 tttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCa                                                          >  2:1072843/1‑141 (MQ=255)
                                                                                 tttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAACTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCa                                                          >  1:197783/1‑141 (MQ=255)
                                                                                  ttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCaa                                                         >  1:219806/1‑141 (MQ=255)
                                                                                       tcCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGc                                                    <  1:966946/141‑1 (MQ=255)
                                                                                         cTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGATCTAAAGGAGGTTACGCCGTTAGAAATTGAAAAGGAGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCaa                                                  >  1:593347/1‑141 (MQ=255)
                                                                                                agagCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGc                                           >  1:1047631/1‑141 (MQ=255)
                                                                                                  agCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGt                                         <  1:1033805/141‑1 (MQ=255)
                                                                                                  agCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGt                                         >  2:370504/1‑141 (MQ=255)
                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATTATATACGTCGAAATGCAATTAAGGCGTc                                        <  2:1087273/141‑1 (MQ=255)
                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTc                                        <  1:391460/141‑1 (MQ=255)
                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTc                                        >  2:444037/1‑141 (MQ=255)
                                                                                                     atatTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTa                                      >  1:493251/1‑141 (MQ=255)
                                                                                                     atatTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTa                                      >  1:569750/1‑141 (MQ=255)
                                                                                                       atTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTaaa                                    >  2:254673/1‑141 (MQ=255)
                                                                                                           aTCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAAttt                                <  2:558220/141‑1 (MQ=255)
                                                                                                              tcAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGa                             >  1:999432/1‑141 (MQ=255)
                                                                                                               cAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGaa                            >  2:129776/1‑141 (MQ=255)
                                                                                                               cAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGaa                            <  1:246728/141‑1 (MQ=255)
                                                                                                                aaTGANAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGNCAAAATGCAATTAAGGCGTCTAAAATTTTGAAt                           <  1:438461/141‑1 (MQ=255)
                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  1:546173/141‑1 (MQ=255)
                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  2:110085/141‑1 (MQ=255)
                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  1:799101/141‑1 (MQ=255)
                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  2:955395/141‑1 (MQ=255)
                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       >  2:641481/1‑141 (MQ=255)
                                                                                                                      aaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCaa                     >  2:88952/1‑141 (MQ=255)
                                                                                                                       aaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCaaa                    >  1:37739/1‑141 (MQ=255)
                                                                                                                        atatAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAAt                   <  1:824637/141‑1 (MQ=255)
                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  <  1:780755/141‑1 (MQ=255)
                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  >  1:1035794/1‑141 (MQ=255)
                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  >  2:411979/1‑141 (MQ=255)
                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTAAGACTAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  >  1:556032/1‑141 (MQ=255)
                                                                                                                             aaTTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATagaga              >  1:101218/1‑141 (MQ=255)
                                                                                                                              aTTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATagaaag             <  1:409908/141‑4 (MQ=255)
                                                                                                                                    cATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACg       <  1:450197/141‑1 (MQ=255)
                                                                                                                                     aTTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACgg      >  2:760839/1‑141 (MQ=255)
                                                                                                                                     aTTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACgg      <  2:979823/141‑1 (MQ=255)
                                                                                                                                      ttGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGt     <  2:1047631/141‑1 (MQ=255)
                                                                                                                                         ttCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTtag  <  1:873734/141‑1 (MQ=255)
                                                                                                                                         ttCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTtag  <  1:411979/141‑1 (MQ=255)
                                                                                                                                         ttCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTtag  <  2:61188/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GACGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTTAG  >  CP000730/182750‑183027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: