Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 211,512 T→A 5.0% intergenic (+70/+258) USA300HOU_0193 → / ← USA300HOU_0194 possible holo‑[acyl‑carrier‑protein] synthase/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730211,5120TA 5.0% 242.7 / 4.0 80intergenic (+70/+258)USA300HOU_0193/USA300HOU_0194possible holo‑[acyl‑carrier‑protein] synthase/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base T (40/35);  new base A (2/2);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GGTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTT  >  CP000730/211373‑211648
                                                                                                                                           |                                                                                                                                        
ggTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttt                                                                                                                                         >  1:491675/1‑141 (MQ=255)
  tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt                                                                                                                                       <  1:995148/141‑1 (MQ=255)
  tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt                                                                                                                                       >  2:12389/1‑141 (MQ=255)
   aTGTTTCTTAGGACAGGCACCCTGGGTTTATAAAAAAGTGTCCGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGtttttt                                                                                                                                      >  1:253561/1‑141 (MQ=255)
    tGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttttt                                                                                                                                     >  1:690320/1‑141 (MQ=255)
    tGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttttt                                                                                                                                     >  1:781349/1‑141 (MQ=255)
      tttCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTAt                                                                                                                                   <  2:257153/141‑1 (MQ=255)
        tCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTAttt                                                                                                                                 >  2:290173/1‑140 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                               <  1:167336/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                               <  1:504852/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGACTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                               >  1:1005696/1‑141 (MQ=255)
           tAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGaa                                                                                                                              <  1:906213/141‑1 (MQ=255)
            aGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTGGaaa                                                                                                                             >  2:977820/1‑141 (MQ=255)
               aCAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAAt                                                                                                                          <  1:542999/141‑1 (MQ=255)
                cAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATg                                                                                                                         <  1:677946/141‑1 (MQ=255)
                  ggCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGaa                                                                                                                       >  1:445470/1‑141 (MQ=255)
                   gCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGaaa                                                                                                                      >  1:992495/1‑141 (MQ=255)
                     aCCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTTGAAAAATGAAAgg                                                                                                                    >  2:656250/1‑141 (MQ=255)
                      cccTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGt                                                                                                                   >  1:461777/1‑141 (MQ=255)
                         tGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGaa                                                                                                                >  1:580792/1‑141 (MQ=255)
                           gggTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATa                                                                                                              <  1:290132/141‑1 (MQ=255)
                               tataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGAAGTCTGACAGTATCATAGCGCAGTGATTGTACCCTATACTGCCTAGAGGGTTACTGTTAGATGTGTTTTTATTGTAAAAGTGAAAGGTGATTACACg                                                                                                          >  1:269829/1‑141 (MQ=255)
                                ataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGa                                                                                                         >  2:126042/1‑141 (MQ=255)
                                ataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGa                                                                                                         >  2:327990/1‑141 (MQ=255)
                                ataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGa                                                                                                         <  2:751925/141‑1 (MQ=255)
                                 taAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGAt                                                                                                        >  2:889478/1‑141 (MQ=255)
                                     aaaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGAGCTCGTAGTTTAAACGATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGca                                                                                                    <  1:19467/141‑1 (MQ=255)
                                      aaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGcat                                                                                                   >  2:887993/1‑141 (MQ=255)
                                       aGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGcatc                                                                                                  >  1:694643/1‑141 (MQ=255)
                                           tCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTc                                                                                              >  1:160454/1‑141 (MQ=255)
                                            cTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCa                                                                                             >  2:665581/1‑141 (MQ=255)
                                               tATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCaaaa                                                                                          <  2:420927/141‑1 (MQ=255)
                                                   tCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAATCTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAt                                                                                      >  2:635252/1‑141 (MQ=255)
                                                   tCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAt                                                                                      <  2:34476/141‑1 (MQ=255)
                                                      gTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTGGATATATGAAAGGTGAATACACGAAGGCATCATTCAAAATTATgtc                                                                                   >  2:29732/1‑139 (MQ=255)
                                                      gTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAAGGAAAGGTGAATACACGATGGCATCATTCAAAATCATGAc                                                                                   >  2:4810/1‑141 (MQ=255)
                                                          ttGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACgaga                                                                               >  1:114237/1‑141 (MQ=255)
                                                          ttGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACgaga                                                                               >  2:239549/1‑141 (MQ=255)
                                                            gAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAtt                                                                             >  1:968945/1‑141 (MQ=255)
                                                                  ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta                                                                       <  1:223769/141‑1 (MQ=255)
                                                                  ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta                                                                       <  1:309267/141‑1 (MQ=255)
                                                                  ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTATAAAAATAGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta                                                                       <  2:965626/141‑1 (MQ=255)
                                                                   tAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagtaa                                                                      >  1:62110/1‑141 (MQ=255)
                                                                    aaTATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTCCTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagtaag                                                                     <  1:919779/141‑1 (MQ=255)
                                                                          aCGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATaa                                                               <  2:1049285/141‑1 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                              >  1:816030/1‑141 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                              >  1:425209/1‑141 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGATTACTGGTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                              >  1:170285/1‑141 (MQ=255)
                                                                              cttaCAGTATCATGGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATTTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTa                                                           <  1:61687/138‑1 (MQ=255)
                                                                              cTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTa                                                           >  2:788859/1‑141 (MQ=255)
                                                                                gACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAATGTaaa                                                         <  1:18093/141‑1 (MQ=255)
                                                                                gACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaa                                                         >  1:1080643/1‑141 (MQ=255)
                                                                                 aCAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaaa                                                        <  2:107404/141‑1 (MQ=255)
                                                                                  cAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaaaa                                                       <  1:363270/141‑1 (MQ=255)
                                                                                      aTCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAAtata                                                   >  1:989982/1‑141 (MQ=255)
                                                                                          ttGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGa                                               <  1:931249/141‑1 (MQ=255)
                                                                                                 gTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAatat                                        <  1:600461/141‑1 (MQ=255)
                                                                                                  tGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATAtt                                       <  2:9040/141‑1 (MQ=255)
                                                                                                       ttAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTGTTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAAt                                  >  1:894670/1‑141 (MQ=255)
                                                                                                       ttAAACTATACTGCATAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGAATCATTCAAAAACATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATAATGgaaa                                  >  2:526498/1‑140 (MQ=255)
                                                                                                           aCTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTAcc                              >  1:392787/1‑141 (MQ=255)
                                                                                                               taCTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTTTTTTAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaa                          >  2:513333/1‑141 (MQ=255)
                                                                                                                tttttcTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                         <  2:40122/136‑1 (MQ=255)
                                                                                                                aCTGGCTAAATATTTACTGTTAGATGTTTTTATATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                         <  2:178701/141‑1 (MQ=255)
                                                                                                                aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                         <  2:375055/141‑1 (MQ=255)
                                                                                                                aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                         <  2:142109/141‑1 (MQ=255)
                                                                                                                 cTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaat                        <  2:939609/141‑1 (MQ=255)
                                                                                                                   gCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaataa                      <  2:1010476/141‑1 (MQ=255)
                                                                                                                    ccTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaataat                     >  1:295492/1‑141 (MQ=255)
                                                                                                                        aaTGTTTTCTGTAAGAAGATTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACAGAGAAATAAACAAAAAAACAAATATAATAGtt                 <  2:766875/141‑1 (MQ=255)
                                                                                                                          tGTTTACTGTTAGATGTTTTTTTTTTTTTTTTTTTAAAGGAGAATACACGCTGGCTTTATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTAAGCAATATTGAAATTACCGATTTTATTAGtttt               >  2:703580/1‑140 (MQ=255)
                                                                                                                          tGTTTACTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAataatatcttg               <  2:696612/141‑6 (MQ=255)
                                                                                                                            tttACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGtt             >  1:939728/1‑141 (MQ=255)
                                                                                                                             ttACTGTTAGATGTTTTTTTTTTTTTTTTATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATGAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTa            >  2:309878/1‑141 (MQ=255)
                                                                                                                             ttACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTa            >  1:115623/1‑141 (MQ=255)
                                                                                                                              tACTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACATGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTac           <  1:415987/141‑1 (MQ=255)
                                                                                                                               aCTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTacg          >  1:352992/1‑141 (MQ=255)
                                                                                                                                cTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacga         <  1:711128/141‑1 (MQ=255)
                                                                                                                                cTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacga         <  2:955671/141‑1 (MQ=255)
                                                                                                                                 tGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTacgac        >  1:485488/1‑141 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATCTACTGCGCAATAGTGAAATTACCGAAAATAATAGTTTGTTacgacg       >  2:555386/1‑141 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg       <  1:338589/141‑1 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg       <  1:75742/141‑1 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGAAAAAAAAAAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg       <  2:445470/141‑1 (MQ=255)
                                                                                                                                       aTGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCAttt  >  1:682973/1‑141 (MQ=255)
                                                                                                                                       aTGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCAttt  >  2:875502/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTT  >  CP000730/211373‑211648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: