Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 321,417 T→C 5.0% N166N (AAT→AAC bsh3 → C59 family penicillin amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730321,4170TC 5.0% 271.8 / 3.0 93N166N (AAT→AACbsh3C59 family penicillin amidase
Reads supporting (aligned to +/- strand):  ref base T (32/55);  new base C (3/2);  total (35/58)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CAAGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGA  >  CP000730/321277‑321551
                                                                                                                                             |                                                                                                                                      
caaGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAAt                                                                                                                                        >  2:352811/1‑141 (MQ=255)
caaGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAAt                                                                                                                                        >  2:610267/1‑141 (MQ=255)
caaGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAAt                                                                                                                                        <  1:854714/141‑1 (MQ=255)
caaGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGAGAAGTGGTTATAAAAGATAAt                                                                                                                                        >  2:305924/1‑141 (MQ=255)
caaGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTGCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAAt                                                                                                                                        >  1:819779/1‑141 (MQ=255)
caaGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACACCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAAt                                                                                                                                        <  1:854680/141‑1 (MQ=255)
   gCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCt                                                                                                                                     <  1:101333/141‑1 (MQ=255)
   gCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCt                                                                                                                                     >  1:394229/1‑141 (MQ=255)
      tCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTAtt                                                                                                                                  >  1:35153/1‑141 (MQ=255)
       cccAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTg                                                                                                                                 <  2:251564/141‑1 (MQ=255)
       cccAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTg                                                                                                                                 >  1:398354/1‑141 (MQ=255)
       cccAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTg                                                                                                                                 >  2:334161/1‑141 (MQ=255)
          aaaTACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGgtg                                                                                                                              >  2:1008418/1‑141 (MQ=255)
                aTGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTaa                                                                                                                        >  2:1046069/1‑141 (MQ=255)
                aTGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTaa                                                                                                                        <  1:733111/141‑1 (MQ=255)
                  gttgtAGCTGTATATTTAAATGTCATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACa                                                                                                                      <  1:85267/141‑1 (MQ=255)
                  gttgtAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACa                                                                                                                      <  1:995819/141‑1 (MQ=255)
                  gttgtAGCTGTATATTTAAATGACATCGGCGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAGGTGGTGATTACCGATAATCCTATTGGTGTCTTAACa                                                                                                                      <  2:107376/141‑1 (MQ=255)
                    tgtATCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACaaa                                                                                                                    <  1:818262/141‑1 (MQ=255)
                    tgtAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACaaa                                                                                                                    <  1:1075255/141‑1 (MQ=255)
                    tgtAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACaaa                                                                                                                    <  2:938475/141‑1 (MQ=255)
                     gtAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATCGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAat                                                                                                                   <  1:294317/141‑1 (MQ=255)
                         cTGTAGATTTAAATGACATCGGTGACGTTCCGCCAGTGCATTATCCTGTTTGCGCTGCAACTGGACATAAAGTCGAAGTTTCAAATAAAGCGGATGAAGGGGTTGTAGAAGATAATGCTGATGATGTCTTAACAAatcgtt                                                                                                               >  2:263579/1‑138 (MQ=255)
                             atatTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGa                                                                                                           <  2:791239/141‑1 (MQ=255)
                                    aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAACTCATCCAGACTTAAAt                                                                                                    <  2:1079244/141‑1 (MQ=255)
                                    aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAt                                                                                                    >  1:597684/1‑141 (MQ=255)
                                    aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAt                                                                                                    >  1:289148/1‑141 (MQ=255)
                                    aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAt                                                                                                    >  1:630230/1‑141 (MQ=255)
                                     aTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATTCAGTCGAAGTTTCATTTAAAGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGAtt                                                                                                   <  2:706443/141‑1 (MQ=255)
                                     aTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAtt                                                                                                   <  2:398092/141‑1 (MQ=255)
                                     aTGACATCGGAGAAGTTACGCCATTGCATAATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAtt                                                                                                   <  2:214411/141‑1 (MQ=255)
                                      tGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTg                                                                                                  >  1:850052/1‑141 (MQ=255)
                                         cATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCa                                                                                               >  2:529683/1‑141 (MQ=255)
                                              gtcatgTTCCGCCATTGCTATATCATGTTTCCGTTCTAACTGGACATACAGTCGAAGTTTCATTTAAAGAGCGTATACTGCTTATAAAAGATAATCTTCTTGGTGTCTTAACAAAGCTTCTGGACTTAATTTGGCATtata                                                                                          <  2:402794/136‑1 (MQ=255)
                                              gTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATTCAGTCGAAGTTTCATTTAAAGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATtata                                                                                          <  2:447146/141‑1 (MQ=255)
                                              gTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATtata                                                                                          <  1:170938/141‑1 (MQ=255)
                                              gTGAAGTTCCGCCATTGCATTATCATGTTTCCGAGGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCACtata                                                                                          <  1:1005488/141‑1 (MQ=255)
                                               tGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAg                                                                                         >  1:1071414/1‑141 (MQ=255)
                                                gAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGt                                                                                        >  2:522109/1‑141 (MQ=255)
                                                gAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGt                                                                                        <  1:302432/141‑1 (MQ=255)
                                                    ttCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAAtt                                                                                    <  1:239453/141‑1 (MQ=255)
                                                    ttCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAAtt                                                                                    <  2:650593/141‑1 (MQ=255)
                                                        gCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAg                                                                                <  1:440907/141‑1 (MQ=255)
                                                               cATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatat                                                                         >  1:841352/1‑141 (MQ=255)
                                                                aTTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatata                                                                        <  2:796724/141‑1 (MQ=255)
                                                                aTTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatata                                                                        >  1:541446/1‑141 (MQ=255)
                                                                 ttATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatatat                                                                       >  2:622054/1‑141 (MQ=255)
                                                                  tATCATGTTTCCGATGCAACTGGACATTCAGTCGAAGTTTCATTTAAAGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATTATAGTAATTTAAGACAATATATc                                                                      >  1:324399/1‑141 (MQ=255)
                                                                  tATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATc                                                                      <  1:241958/141‑1 (MQ=255)
                                                                   aTCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCa                                                                     <  2:133307/141‑1 (MQ=255)
                                                                    tCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCaa                                                                    <  1:464298/141‑1 (MQ=255)
                                                                     cATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAat                                                                   >  1:586773/1‑141 (MQ=255)
                                                                     cATGTTTCCGATGCAACTGGACATACAGGCGAAGTTTCAATTCATTAGGGCGTAGTAGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGTCATTATAGTAATTTAAGAGAATATATCaaa                                                                   >  2:500195/1‑140 (MQ=255)
                                                                        tattCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCGATAtt                                                                <  2:337014/139‑1 (MQ=255)
                                                                        gTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGAATTATAGTAATTTAAGACAATATATCAATAtt                                                                >  1:1018340/1‑141 (MQ=255)
                                                                         tttCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATAttt                                                               <  1:364442/141‑1 (MQ=255)
                                                                         tttCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATAttt                                                               <  2:817283/141‑1 (MQ=255)
                                                                         tttCCGAGTCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGCTATAAAAGATAATGCTAGTGGTGTCGTAACAAGTCGTCCAGACTTAAATTTTCATTATAGTAATTGAAGACAATATATCAATAttt                                                               <  2:864343/141‑1 (MQ=255)
                                                                            ccGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTtctc                                                            <  1:864768/141‑1 (MQ=255)
                                                                               aTGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCtt                                                         >  2:707474/1‑141 (MQ=255)
                                                                                tGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTa                                                        >  2:656700/1‑141 (MQ=255)
                                                                                 gCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTAt                                                       <  2:773245/141‑1 (MQ=255)
                                                                                     cTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAAGCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcag                                                   <  2:423394/141‑1 (MQ=255)
                                                                                      tGGACATACAGTCGAAGTTTCAATTAAAGAGGGTGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATAcaga                                                  >  2:1064795/1‑140 (MQ=255)
                                                                                        gACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaa                                                >  2:544271/1‑141 (MQ=255)
                                                                                            tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                            <  2:551644/141‑1 (MQ=255)
                                                                                            tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                            <  2:244160/141‑1 (MQ=255)
                                                                                            tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                            >  1:263063/1‑141 (MQ=255)
                                                                                            tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                            <  2:228973/141‑1 (MQ=255)
                                                                                            tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                            <  1:533233/141‑1 (MQ=255)
                                                                                            tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                            <  1:188334/141‑1 (MQ=255)
                                                                                             aCAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagca                                           <  1:498209/141‑1 (MQ=255)
                                                                                              cAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagcaa                                          >  1:981682/1‑141 (MQ=255)
                                                                                                gTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAACAGATAATCCTATTGGTGTCTTAACCAATCATCCAGACTTCAATTGGCACTATAGTCATCTAAGAAAATATATCAATATTTCTCCTGATCCAGCAACAGCAAAt                                        <  1:158475/141‑1 (MQ=255)
                                                                                                gTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAAt                                        >  2:174107/1‑141 (MQ=255)
                                                                                                   gAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTa                                     <  1:649390/141‑1 (MQ=255)
                                                                                                    aaGTTTAATTTAGAAGAGGGTGAGGTGGTTATACAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAGTATTTTGCCTTATCCGGCAACAGCAAATTTa                                     <  2:322961/141‑1 (MQ=255)
                                                                                                     aGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTAtt                                   >  2:86211/1‑141 (MQ=255)
                                                                                                      ggttCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTCTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACACTATATCAATATTTCTCCTTATCCAGCAACAGAAAAATTATTg                                  >  2:915218/3‑141 (MQ=255)
                                                                                                      gTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATCGGTGACTTAACAAATCATCAAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATATATTg                                  >  2:359320/1‑141 (MQ=255)
                                                                                                           aTTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAgg                             <  2:324880/141‑1 (MQ=255)
                                                                                                           aTTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAgg                             <  2:128276/141‑1 (MQ=255)
                                                                                                               aaaGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGCTCTCCTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTa                         >  1:1062830/1‑141 (MQ=255)
                                                                                                                 aaGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAAGTTATTGGAAGGTGTaa                        >  2:825696/1‑141 (MQ=255)
                                                                                                                    agGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGa                     <  2:817242/141‑1 (MQ=255)
                                                                                                                    agGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGa                     <  2:204125/141‑1 (MQ=255)
                                                                                                                    agGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGa                     <  1:328849/141‑1 (MQ=255)
                                                                                                                      ggTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGAtt                   <  2:730995/141‑1 (MQ=255)
                                                                                                                      ggTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGAtt                   <  2:819779/141‑1 (MQ=255)
                                                                                                                      ggTGAAGTGGTTATAAAAGATAATCCTATTGGTGTATTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGAtt                   <  2:948928/141‑1 (MQ=255)
                                                                                                                       gTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTg                  <  2:904691/141‑1 (MQ=255)
                                                                                                                       gTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAAAGATTg                  >  1:454/1‑141 (MQ=255)
                                                                                                                       gTGAAGTGGTTATAAAAGATAAGCCTATTGGTGTCTTAACAAGTCATCCAGACTTGAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCGGCAACAGCAAATTTATTGGAAGGTGTAACGATTg                  <  1:1008019/141‑1 (MQ=255)
                                                                                                                        tGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGa                 <  1:431074/141‑1 (MQ=255)
                                                                                                                                 tataAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAgg        >  1:760875/1‑141 (MQ=255)
                                                                                                                                  ataAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTCACGATTGAACCTTTAGGc       <  2:495300/141‑1 (MQ=255)
                                                                                                                                  ataAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGc       <  1:975895/141‑1 (MQ=255)
                                                                                                                                    aaaaGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCaa     <  1:619137/141‑1 (MQ=255)
                                                                                                                                       aGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGa  <  1:825959/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                      
CAAGCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGA  >  CP000730/321277‑321551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: