Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 323,222 G→A 6.5% intergenic (+34/+18) lytM → / ← USA300HOU_0290 M23 family lysostaphin/ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730323,2220GA 6.5% 288.1 / 4.2 95intergenic (+34/+18)lytM/USA300HOU_0290M23 family lysostaphin/ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base G (44/44);  new base A (4/3);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTACGGGTAATTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAATGATGGGTAGTTAAT  >  CP000730/323084‑323360
                                                                                                                                           |                                                                                                                                          
agtaCGGGTAATTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCCAAGTAGAGAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCt                                                                                                                                          <  1:248084/141‑1 (MQ=255)
 gtaCGGGTAATTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCtt                                                                                                                                         <  2:211033/141‑1 (MQ=255)
   aCGGGTAATTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCCTACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTgg                                                                                                                                       <  1:959023/141‑1 (MQ=255)
     gggTAATTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGa                                                                                                                                     <  1:256779/141‑1 (MQ=255)
          aTTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGAttttt                                                                                                                                <  1:2327/141‑1 (MQ=255)
          aTTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGAttttt                                                                                                                                <  2:385308/141‑1 (MQ=255)
           ttCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTTATACTTGCAAAGTAGATAATACAGAATATCCCTAGCTGCGTATGATATCGCAACTTGGGAttttct                                                                                                                               <  2:179678/141‑3 (MQ=37)
            tCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTc                                                                                                                              >  1:53744/1‑141 (MQ=255)
              aaCAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGt                                                                                                                            >  2:104888/1‑141 (MQ=255)
               aCAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGGTAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGtt                                                                                                                           <  1:233094/141‑1 (MQ=255)
               aCAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGtt                                                                                                                           <  1:347429/141‑1 (MQ=255)
               aCAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGtt                                                                                                                           >  2:144297/1‑141 (MQ=255)
               aCAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGtt                                                                                                                           >  1:27924/1‑141 (MQ=255)
                cAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGttt                                                                                                                          <  2:992464/141‑1 (MQ=255)
                  gcgcCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTta                                                                                                                        <  2:622308/141‑1 (MQ=255)
                      cTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGAGCCAACGTCATACTTGCAAAGTAGATAATACAGTAAATCCCAAGTTACAATATCATACGCAACTTGGGATTTTTTCGTTTtaataa                                                                                                                    <  2:334576/141‑1 (MQ=255)
                      cTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTtaataa                                                                                                                    <  2:76142/141‑1 (MQ=255)
                       tCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTaataat                                                                                                                   >  2:305028/1‑141 (MQ=255)
                         aCGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAg                                                                                                                 >  2:96263/1‑141 (MQ=255)
                           gTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGt                                                                                                               >  1:875432/1‑141 (MQ=255)
                           gTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGt                                                                                                               >  1:928979/1‑141 (MQ=255)
                             acacTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGtat                                                                                                             <  2:637229/141‑1 (MQ=255)
                              cacTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATAGTTGCAAAGTAGATAATACAGAAAACCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGtata                                                                                                            <  1:246374/141‑1 (MQ=255)
                                cTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAg                                                                                                          <  2:955170/141‑1 (MQ=255)
                                 ttCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGt                                                                                                         <  1:530425/141‑1 (MQ=255)
                                  tCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTc                                                                                                        >  2:445890/1‑141 (MQ=255)
                                    cAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCa                                                                                                      <  1:163515/141‑1 (MQ=255)
                                    cAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCa                                                                                                      <  1:769326/141‑1 (MQ=255)
                                      aCGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCAtt                                                                                                    >  2:195395/1‑141 (MQ=255)
                                       cGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCAttg                                                                                                   <  2:828566/141‑1 (MQ=255)
                                          aTGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCAttgttt                                                                                                >  1:412178/1‑141 (MQ=255)
                                             tCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTc                                                                                             >  1:2150/1‑141 (MQ=255)
                                                  tggCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTa                                                                                        <  1:324421/141‑1 (MQ=255)
                                                     cATCGGTAATCAATATGCAGTAGACCCACCGTCATACTTGCAAAGTAGATAATACAGAAAAGCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTaaaa                                                                                     <  2:547146/141‑1 (MQ=255)
                                                     cATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTaaaa                                                                                     >  1:750458/1‑141 (MQ=255)
                                                     cATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTaaaa                                                                                     >  1:817942/1‑141 (MQ=255)
                                                         ggTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATg                                                                                 >  1:313126/1‑141 (MQ=255)
                                                         gattaTCAATATGCGGTTGTCACAACGTCATACTTGCAATGTTGATAATATTGAAAATCCCAAATAGCGATATCATACGCAGCTTGGCATTTTTTCGTTTTAATAATAGGTATACGTCCATTTTTTGTCCTCTAAAAAATg                                                                                 <  2:977531/137‑1 (MQ=255)
                                                          gTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGt                                                                                >  2:739224/1‑141 (MQ=255)
                                                          gTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGt                                                                                >  2:45260/1‑141 (MQ=255)
                                                            aaTCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGCTGCGAAATAATACGCAACTTGGGATTTTTTTTTTTTTTTTATAGGTATAATTCCATTGTTTTTGTTCTTAAACATAttt                                                                              >  2:995612/1‑140 (MQ=255)
                                                             aTCAATATGCAGTAGCCCGGCCGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGa                                                                             <  1:283614/141‑1 (MQ=255)
                                                             aTCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCGTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGa                                                                             <  2:963654/141‑1 (MQ=255)
                                                              tCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGAt                                                                            <  1:581568/141‑1 (MQ=255)
                                                              tCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGAt                                                                            <  1:641760/141‑1 (MQ=255)
                                                               cAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATa                                                                           >  2:840659/1‑141 (MQ=255)
                                                               cAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATa                                                                           >  1:459485/1‑141 (MQ=255)
                                                               cAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATa                                                                           >  2:242038/1‑141 (MQ=255)
                                                                   atGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATaaaaa                                                                       <  1:129383/141‑1 (MQ=255)
                                                                      cAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATaaaccgca                                                                    >  1:817561/1‑136 (MQ=255)
                                                                      cAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGa                                                                    >  2:735954/1‑141 (MQ=255)
                                                                        ggaGACCCAACGTCATACGTGCAAAGTAGATAATACAGAAAAGCCCAAGTTGCGATATCATACGCTGCTTGGGATTTTTGCGTTTTAATAATATTTATAAGTCCATTGTTTGTCCTCTAAAACATGGTGATAAAAAGGATc                                                                  <  2:974411/139‑1 (MQ=255)
                                                                        gTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAGATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATc                                                                  >  2:895076/1‑141 (MQ=255)
                                                                        gTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATc                                                                  >  1:661884/1‑141 (MQ=255)
                                                                           gACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTCTTAATAATAGGTATAAGTCCATTGTTTCTCCTCTAAAACATCTTGATAAAAAGGATCTTg                                                               >  1:937443/1‑141 (MQ=255)
                                                                              ccAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGcc                                                            >  1:90017/1‑141 (MQ=255)
                                                                              ccAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGcc                                                            >  1:89970/1‑141 (MQ=255)
                                                                              ccAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGcc                                                            <  2:866761/141‑1 (MQ=255)
                                                                                 aCGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCGTACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAAt                                                         <  2:27428/141‑1 (MQ=255)
                                                                                 aCGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAAt                                                         <  1:592469/141‑1 (MQ=255)
                                                                                 aCGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAAt                                                         >  1:1016897/1‑141 (MQ=255)
                                                                                     cATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAAc                                                     <  1:656979/141‑1 (MQ=255)
                                                                                     cATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAAc                                                     >  2:1078541/1‑141 (MQ=255)
                                                                                     cATACTTGCAAAGTAGATAATACAGAAAATCCAAAGTTGCGCTATCATACGCGGCTTGGGATTTTTTCGTTTTAATGCTAGGTATAAGTCCATTGTTTGTCCTGTAAAACATGTTGATAAAAAGGTTCTTGGCCAGTTatc                                                     >  2:1078543/1‑139 (MQ=255)
                                                                                       tACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACtt                                                   >  1:534828/1‑141 (MQ=255)
                                                                                       tACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACtt                                                   >  1:458201/1‑141 (MQ=255)
                                                                                        aCTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTg                                                  >  2:516688/1‑141 (MQ=255)
                                                                                               aaGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATatcat                                           >  2:413178/1‑141 (MQ=255)
                                                                                               aaGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATatcat                                           >  1:480387/1‑141 (MQ=255)
                                                                                                 gTAGATAATACAGAAAATCTCAAGTTGCGATATCATACGCAGCTAGGGAGTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAGAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCt                                         >  2:1006681/1‑141 (MQ=255)
                                                                                                   aGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGc                                       <  1:751837/141‑1 (MQ=255)
                                                                                                   aGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGc                                       <  2:473545/141‑1 (MQ=255)
                                                                                                   aGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGc                                       <  1:823395/141‑1 (MQ=255)
                                                                                                   aGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAAAAAATTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGc                                       <  2:364846/141‑1 (MQ=255)
                                                                                                     ataataCAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCaa                                     <  1:953404/141‑1 (MQ=255)
                                                                                                      taataCAGAATATCCCATGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAg                                    <  2:1053610/141‑1 (MQ=255)
                                                                                                         taCAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGc                                 <  2:797988/141‑1 (MQ=255)
                                                                                                          aCAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCt                                <  2:361335/141‑1 (MQ=255)
                                                                                                          aCAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTGGATATCATCTGCAAGTGCt                                <  1:89695/141‑1 (MQ=255)
                                                                                                             gAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCa                             >  2:272958/1‑141 (MQ=255)
                                                                                                                   cccAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCa                       >  1:84542/1‑141 (MQ=255)
                                                                                                                    ccAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCAt                      <  1:681378/141‑1 (MQ=255)
                                                                                                                    ccAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGGTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCAt                      >  1:514660/1‑141 (MQ=255)
                                                                                                                    ccAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACAAGTTAAAAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCAt                      <  2:140075/141‑1 (MQ=255)
                                                                                                                     cAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATc                     >  1:711894/1‑141 (MQ=255)
                                                                                                                      aaGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCACCTGCAAGTGCTTCAACTTCATCt                    >  2:137294/1‑141 (MQ=255)
                                                                                                                          ctgcgtatgatatcGCAACTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTaaa                 >  1:216786/14‑141 (MQ=255)
                                                                                                                           gCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAatg               <  1:242038/141‑1 (MQ=255)
                                                                                                                            gcgtatgatatcGCAACTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAatg               >  1:231306/12‑141 (MQ=255)
                                                                                                                              atatCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAatgatg            <  1:43873/141‑1 (MQ=255)
                                                                                                                               tatgatatcGCAACTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAatgatg            >  2:1074431/9‑141 (MQ=255)
                                                                                                                                  cATACGCAACTTGGGATTTTTTCGTTTTAATAATAGGTATAAGCCCATTGCTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCCACTTCATCTAAATGATGGGTa        <  1:269349/141‑1 (MQ=255)
                                                                                                                                       cGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAATGACGGGAAGTTa    >  1:154119/1‑141 (MQ=255)
                                                                                                                                         cAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAATGATGGGTAGTTAat  >  1:867723/1‑141 (MQ=255)
                                                                                                                                         cAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGAACTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAATGATGGGTAGTTAat  >  2:448261/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGTACGGGTAATTCAACAGCGCCTCACGTACACTTCCAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAAAGTAGATAATACAGAAAATCCCAAGTTGCGATATCATACGCAGCTTGGGATTTTTTCGTTTTAATAATAGGTATAAGTCCATTGTTTGTCCTCTAAAACATGTTGATAAAAAGGATCTTGGCCAATTAACTTGATATCATCTGCAAGTGCTTCAACTTCATCTAAATGATGGGTAGTTAAT  >  CP000730/323084‑323360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: