BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	326,758	A→T	5.6%	F277Y (TTT→TAT)	USA300HOU_0293 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	326,758	0	A	T	5.6%	253.6 / 3.6	93	F277Y (TTT→TAT)	USA300HOU_0293	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (41/43); new base T (4/2); total (47/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTATTTTCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTACTTTT  >  CP000730/326619‑326896

attattTTCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCaaa                                                                                                                                           <  1:811578/141‑1 (MQ=255)

attattTTCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCaaa                                                                                                                                           >  1:993118/1‑141 (MQ=255)

 ttattTTCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCtaat                                                                                                                                          >  2:661040/1‑140 (MQ=255)

      ttCTTTGTCTGTAATAGTAATTTCTCGTGTTACAGTCGTATGTTAATGGGTATAAGTTTTAGATGGAGTTTTAGCAAATTCATCATCTTTATTTTCTTTGACGTCGTTAATTGCTTGTGTGATGAGTTTTTCAGAGTCtgt                                                                                                                                     >  2:521729/1‑139 (MQ=255)

       tCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGTTGTCTTTTTCAAAGTCtttt                                                                                                                                    >  1:692206/1‑141 (MQ=255)

       tCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCtttt                                                                                                                                    >  1:439677/1‑141 (MQ=255)

         tttGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGt                                                                                                                                  <  1:717682/141‑1 (MQ=255)

          ttGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTATTTTGTa                                                                                                                                 >  2:1062575/1‑141 (MQ=255)

           tGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAg                                                                                                                                >  2:255663/1‑141 (MQ=255)

           tGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAg                                                                                                                                <  2:466581/141‑1 (MQ=255)

               tGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCAcc                                                                                                                            <  1:502931/141‑1 (MQ=255)

                gTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTCCTTTGACATTGTTCGTTGTTACTACGTTGTCTTTCTCAACCTCTTTTGTatcctct                                                                                                                           >  1:762709/1‑135 (MQ=255)

                  aaTAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTg                                                                                                                         >  2:146710/1‑141 (MQ=255)

                    tAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGtt                                                                                                                       <  2:119480/141‑1 (MQ=255)

                    tAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGtt                                                                                                                       >  1:395768/1‑141 (MQ=255)

                        aaTTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTNGttcttc                                                                                                                   >  2:815483/1‑141 (MQ=255)

                         aTTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTcc                                                                                                                  >  2:697566/1‑141 (MQ=255)

                           ttCTCGTTTTACAATCGTATGTTTTTTTTTTTTTTTTTTAGATTGAATTTTAGCAAATTCATCTTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATTTCCTTATCATATTCTTTTTTAGCACCTTGttcttcttt                                                                                                                >  2:805551/1‑138 (MQ=255)

                              tcGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTaa                                                                                                             >  1:807279/1‑141 (MQ=255)

                               cGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCATAGTCTTTTGTAGCACCTTGTTCTTCCATTAAt                                                                                                            >  2:983596/1‑141 (MQ=255)

                                gTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAAtt                                                                                                           >  1:996906/1‑141 (MQ=255)

                                  tttACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAAtttt                                                                                                         <  2:499183/141‑1 (MQ=255)

                                  tttACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAAtttt                                                                                                         >  1:886327/1‑141 (MQ=255)

                                    tACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAAtttttt                                                                                                       <  1:600984/141‑1 (MQ=255)

                                    tACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAAtttttt                                                                                                       <  2:366393/141‑1 (MQ=255)

                                     aCAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTATTTTCTTTTTTATTGTCAATTTCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAAttttttt                                                                                                      >  2:446435/1‑140 (MQ=255)

                                     aCAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTa                                                                                                      >  2:236871/1‑141 (MQ=255)

                                      cAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTaa                                                                                                     >  1:953896/1‑141 (MQ=255)

                                       aaTCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAg                                                                                                    <  2:903951/141‑1 (MQ=255)

                                       aaTCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAg                                                                                                    <  2:856700/141‑1 (MQ=255)

                                        aTCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGGTGCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTGTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAgg                                                                                                   <  1:782340/141‑1 (MQ=255)

                                        aTCGTATGTTTTTCGGTATAAATTTTAGATCGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAgg                                                                                                   >  2:977687/1‑141 (MQ=255)

                                         tCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGt                                                                                                  >  1:527094/1‑141 (MQ=255)

                                          cGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTTAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTc                                                                                                 >  1:309322/1‑141 (MQ=255)

                                          cGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCACAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTGAGGTc                                                                                                 <  2:241663/141‑1 (MQ=255)

                                          cGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTc                                                                                                 <  1:580681/141‑1 (MQ=255)

                                              tgtgTTTCGGTATATATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTa                                                                                             <  1:251295/141‑1 (MQ=255)

                                              tGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTa                                                                                             <  1:251320/141‑1 (MQ=255)

                                                 ttttCGGTATAACTTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATcc                                                                                          <  2:1037076/141‑1 (MQ=255)

                                                  tttCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCt                                                                                         <  2:719834/141‑1 (MQ=255)

                                                   ttCGGTATAAATTTTCGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCCTTATCATAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCtt                                                                                        <  1:898558/141‑1 (MQ=255)

                                                   ttCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCtt                                                                                        <  1:677182/141‑1 (MQ=255)

                                                   ttCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCtt                                                                                        <  2:788039/141‑1 (MQ=255)

                                                     cGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCtttt                                                                                      >  1:258267/1‑141 (MQ=255)

                                                       gTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTa                                                                                    <  1:203649/141‑1 (MQ=255)

                                                            aaTTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGcttc                                                                               <  2:913625/141‑1 (MQ=255)

                                                                ttAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTTTTTATCCTTTTTAGCTtcttct                                                                           >  2:46343/1‑141 (MQ=255)

                                                                ttAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTtcttct                                                                           >  2:839797/1‑141 (MQ=255)

                                                                 tAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTTATTTCTTTTTTAAAGTCTTTTGTAGCACCTTGTTCTTCCATTTATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTa                                                                          >  2:176655/1‑141 (MQ=255)

                                                                 tAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTa                                                                          >  1:372010/1‑141 (MQ=255)

                                                                  aGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTaa                                                                         <  1:394925/141‑1 (MQ=255)

                                                                  aGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCCTTATCATAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTaa                                                                         >  1:1636/1‑141 (MQ=255)

                                                                       gAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAg                                                                    <  2:434118/141‑1 (MQ=255)

                                                                         aTTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACTGCt                                                                  >  2:969204/1‑141 (MQ=255)

                                                                           tttAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTaa                                                                <  2:61951/141‑1 (MQ=255)

                                                                            ttAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTTCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAAt                                                               <  1:1061900/141‑1 (MQ=255)

                                                                               gCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTa                                                            >  1:883297/1‑141 (MQ=255)

                                                                               gCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTa                                                            <  1:709401/141‑1 (MQ=255)

                                                                               gCAAATTCATCCTTTTTAGTTTCTATGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTGTTCCATTAATTTTTTAAGGTGTTTATCCTTTTTGGCTTCTTCTAGTACCGCTAATGTa                                                            <  2:915087/141‑1 (MQ=255)

                                                                                 aaaTTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTTTTTTTTTTTTTAAAGTCTTTTGTAGCACCTTGTTTTTCCATTTATTTTTTAAGGTCTTTATCCTTTTTATCTTCTTCTATTATAGCTAATGTAAt                                                          >  2:78160/1‑141 (MQ=255)

                                                                                 aaaTTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAAt                                                          <  2:302182/141‑1 (MQ=255)

                                                                                 aaaTTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTAACCTTTTTAGCTTCTTCTAATACAGCTAATGTAAt                                                          >  1:1006577/1‑141 (MQ=255)

                                                                                    ttCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTATCTTCTTCTAATACAGCTAATGTACtttt                                                       >  2:35316/1‑141 (MQ=255)

                                                                                    ttCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAAtttt                                                       >  1:599233/1‑141 (MQ=255)

                                                                                        ttcTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTa                                                   <  2:654883/139‑1 (MQ=255)

                                                                                         ccTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAg                                                  >  1:686139/1‑141 (MQ=255)

                                                                                               tAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAg                                            <  1:150694/141‑1 (MQ=255)

                                                                                               tAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAg                                            <  1:419835/141‑1 (MQ=255)

                                                                                                aGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGc                                           <  2:627866/141‑1 (MQ=255)

                                                                                                 gTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGct                                          <  1:842023/141‑1 (MQ=255)

                                                                                                 gTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTGCTAATACAGCTAATGTAATTTTTTTAGTGTCAGct                                          <  1:842022/141‑1 (MQ=255)

                                                                                                    tCTTTGACATCGTCAATTGCTTTTTTGATTTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGctctt                                       >  2:971395/1‑140 (MQ=255)

                                                                                                    tCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCTTTGTTTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTg                                       >  2:298680/1‑141 (MQ=255)

                                                                                                    tCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCACAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACCGCTAATGTAAttttttttttttcttctctt                                       >  2:859442/1‑128 (MQ=255)

                                                                                                         gACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAg                                  <  1:291279/141‑1 (MQ=255)

                                                                                                          aCATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGcttctt                                                                             >  2:1016640/1‑97 (MQ=255)

                                                                                                          aCATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGcttctt                                                                             <  1:1016640/97‑1 (MQ=255)

                                                                                                             tCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGtt                              >  1:624645/1‑141 (MQ=255)

                                                                                                              cGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTa                             <  1:375529/141‑1 (MQ=255)

                                                                                                                   aTTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTg                        >  1:926147/1‑141 (MQ=255)

                                                                                                                     tGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGAc                      >  1:416969/1‑141 (MQ=255)

                                                                                                                     tGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGAc                      <  2:707493/141‑1 (MQ=255)

                                                                                                                      gCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACa                     >  2:479975/1‑141 (MQ=255)

                                                                                                                        ttttttGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAgg                   <  1:839797/141‑1 (MQ=255)

                                                                                                                        ttttttGATGTCCTTATCATAGTCTTTTGTAGCACCTTCTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAgg                   <  1:314125/141‑1 (MQ=255)

                                                                                                                           tttGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCt                <  2:47289/141‑1 (MQ=255)

                                                                                                                            ttGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTa               >  1:579886/1‑141 (MQ=255)

                                                                                                                                 gTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGTTCTAACttt          >  2:535662/1‑141 (MQ=255)

                                                                                                                                  tCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTg         <  2:920439/141‑1 (MQ=255)

                                                                                                                                  tCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTg         <  1:941153/141‑1 (MQ=255)

                                                                                                                                    tttttCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTa       <  1:925547/141‑1 (MQ=255)

                                                                                                                                       ttCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGTTCTAACTTTGTACtt    >  2:970994/1‑141 (MQ=255)

                                                                                                                                       ttCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTACtt    >  1:261990/1‑141 (MQ=255)

                                                                                                                                        tCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTACttt   >  2:982925/1‑141 (MQ=255)

                                                                                                                                         cAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTACtttt  <  1:221827/141‑1 (MQ=255)

                                                                                                                                         cAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTACtttt  <  2:949033/141‑1 (MQ=255)

ATTATTTTCTTTGTCTGTAATAGTAATTTCTCGTTTTACAATCGTATGTTTTTCGGTATAAATTTTAGATTGAATTTTAGCAAATTCATCCTTTTTAGTTTCTTTGACATCGTCAATTGCTTTTTTGATGTCTTTTTCAAAGTCTTTTGTAGCACCTTGTTCTTCCATTAATTTTTTAAGGTCTTTATCCTTTTTAGCTTCTTCTAATACAGCTAATGTAATTTTTTTAGTGTCAGCTCTGCTAAGTGTTAACGTGACAGGTCTAACTTTGTACTTTT  >  CP000730/326619‑326896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`