Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	340,266	A→G	7.2%	A1468A (GCA→GCG)	essC →	virulence protein EssC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	340,266	0	A	G	7.2%	263.7 / 6.7	93	A1468A (GCA→GCG)	essC	virulence protein EssC
Reads supporting (aligned to +/- strand):  ref base A (43/43);  new base G (3/4);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAG  >  CP000730/340126‑340400                                                                                                                                             |                                                                                                                                       tATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCa                                                                                                                                        >  2:656304/1‑141 (MQ=255)   tGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCaaa                                                                                                                                      >  2:293261/1‑141 (MQ=255)       aaaCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCaa                                                                                                                                  >  2:934604/1‑141 (MQ=255)         aCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGc                                                                                                                                <  1:681864/141‑1 (MQ=255)         aCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGc                                                                                                                                <  1:604849/141‑1 (MQ=255)           aGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCtt                                                                                                                              <  1:885382/141‑1 (MQ=255)            gATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTa                                                                                                                             >  1:346508/1‑141 (MQ=255)              ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGTCCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                                           <  1:646306/141‑1 (MQ=255)              ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                                           <  1:759011/141‑1 (MQ=255)              ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                                           >  1:330797/1‑141 (MQ=255)               tGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCa                                                                                                                          >  2:1050256/1‑141 (MQ=255)                gATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCaa                                                                                                                         <  1:1048936/141‑1 (MQ=255)                   tttGCACNTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTCAAATTTAGATTAATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATAGTCGCAAACCAAGCTTATCAAAAg                                                                                                                      <  2:814439/140‑1 (MQ=255)                   gTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAg                                                                                                                      <  2:630391/141‑1 (MQ=255)                      gCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGAtt                                                                                                                   <  2:172934/141‑1 (MQ=255)                      gCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGAtt                                                                                                                   <  2:1037444/141‑1 (MQ=255)                         cGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGa                                                                                                                <  1:532827/141‑1 (MQ=255)                             aaaTGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGt                                                                                                            <  2:629606/141‑1 (MQ=255)                             aaaTGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGt                                                                                                            >  2:1012616/1‑141 (MQ=255)                                 gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                                        >  2:120142/1‑141 (MQ=255)                                 gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                                        <  2:781604/141‑1 (MQ=255)                                 gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                                        >  1:585893/1‑141 (MQ=255)                                  aTTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaaa                                                                                                       >  1:189720/1‑141 (MQ=255)                                   ttAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAAt                                                                                                      <  2:745538/141‑1 (MQ=255)                                   ttAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAAt                                                                                                      <  2:332311/141‑1 (MQ=255)                                     aaCCAATTTAGTATATGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCTTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAg                                                                                                    <  1:411277/141‑1 (MQ=255)                                     aaCCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAg                                                                                                    <  1:411365/141‑1 (MQ=255)                                        cAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCaa                                                                                                 >  1:148899/1‑141 (MQ=255)                                          aTTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATg                                                                                               <  2:413284/141‑1 (MQ=255)                                           tttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGa                                                                                              >  1:435798/1‑141 (MQ=255)                                            ttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGaa                                                                                             >  2:866594/1‑141 (MQ=255)                                            ttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGaa                                                                                             >  2:32314/1‑141 (MQ=255)                                              aGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAAtt                                                                                           <  2:31327/141‑1 (MQ=255)                                              aGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAAtt                                                                                           <  2:31376/141‑1 (MQ=255)                                                  taGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAAt                                                                                       <  2:504951/141‑1 (MQ=255)                                                    ggTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATag                                                                                     <  2:23317/141‑1 (MQ=255)                                                       aTTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATaggag                                                                                  >  2:239750/1‑141 (MQ=255)                                                         tCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggg                                                                                >  2:426875/1‑141 (MQ=255)                                                         tCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggg                                                                                <  2:124162/141‑1 (MQ=255)                                                          cGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggga                                                                               <  2:614027/141‑1 (MQ=255)                                                           gTATTTCAGACCACCAATTCTTTCAATTTAGATTTATTCAACGGCAAGCTTTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggagggag                                                                              <  1:585598/141‑1 (MQ=255)                                                           gTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAAACAAGATTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggagggag                                                                              >  1:701982/1‑141 (MQ=255)                                                              tttCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTa                                                                           <  1:215862/141‑1 (MQ=255)                                                              tttCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTa                                                                           <  1:215900/141‑1 (MQ=255)                                                                tCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATg                                                                         <  2:502518/141‑1 (MQ=255)                                                                tCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATg                                                                         <  2:336514/141‑1 (MQ=255)                                                                    aCCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTGAATAGGAGGGAGGTATGTTAt                                                                     >  2:918011/1‑141 (MQ=255)                                                                     ccAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATg                                                                    >  1:492990/1‑141 (MQ=255)                                                                          aaTTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAAttt                                                               >  1:1008493/1‑141 (MQ=255)                                                                            ttCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTa                                                             <  2:726202/141‑1 (MQ=255)                                                                             tCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTaa                                                            >  1:811610/1‑141 (MQ=255)                                                                              cTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAAt                                                           <  1:823348/141‑1 (MQ=255)                                                                                ttAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGa                                                         >  2:924533/1‑141 (MQ=255)                                                                                ttAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGa                                                         >  2:890172/1‑141 (MQ=255)                                                                                 tAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGat                                                        >  1:645917/1‑141 (MQ=255)                                                                                       tAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTACATAGGCGGGAGGTATGTTATGAATTTTAATGATATTGaa                                                  <  2:772061/141‑1 (MQ=255)                                                                                        aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                                 >  2:1029942/1‑141 (MQ=255)                                                                                        aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                                 >  2:911368/1‑141 (MQ=255)                                                                                        aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                                 >  1:82643/1‑141 (MQ=255)                                                                                        aGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                                 <  2:278669/141‑1 (MQ=255)                                                                                        aGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                                 >  2:139775/1‑141 (MQ=255)                                                                                         gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAAc                                                <  2:138364/141‑1 (MQ=255)                                                                                         gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAAc                                                <  1:872065/141‑1 (MQ=255)                                                                                         gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGCATGTTATGAATTTTAATGATATTGAAAc                                                >  2:983063/1‑141 (MQ=255)                                                                                                 cAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTa                                        <  2:196713/141‑1 (MQ=255)                                                                                                 cAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGCTATGTTATGAATTTTAATGATATTGAAACAATGGTTa                                        <  1:785049/141‑1 (MQ=255)                                                                                                   aCGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAg                                      >  1:862912/1‑141 (MQ=255)                                                                                                   aCGAGAACCTGTTATTAAAGAAAATGAAGCATACACGGTCGCAAACCAAGATTCTCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAg                                      >  2:593635/1‑141 (MQ=255)                                                                                                     gagaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTc                                    <  2:359229/141‑1 (MQ=255)                                                                                                     gagaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTc                                    <  1:41422/141‑1 (MQ=255)                                                                                                      agaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCg                                   <  1:622868/141‑1 (MQ=255)                                                                                                      agaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCg                                   <  2:527587/141‑1 (MQ=255)                                                                                                       gaACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAGTTTTAATGATATTGAAACAATGGTCAAGTCGa                                  >  1:930400/1‑141 (MQ=255)                                                                                                       gaACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTCAAGTCGa                                  >  1:930450/1‑141 (MQ=255)                                                                                                         aCCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGaaa                                >  2:132203/1‑141 (MQ=255)                                                                                                         aCCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGaaa                                >  1:61843/1‑141 (MQ=255)                                                                                                          ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                               >  2:673993/1‑141 (MQ=255)                                                                                                          ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                               >  2:482835/1‑141 (MQ=255)                                                                                                          ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                               >  2:531337/1‑141 (MQ=255)                                                                                                           cTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAtt                              >  2:161961/1‑141 (MQ=255)                                                                                                           cTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAtt                              >  1:430977/1‑141 (MQ=255)                                                                                                                   aaaGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGata                      <  1:167206/141‑1 (MQ=255)                                                                                                                     aGAAAATGAAGCATATATGGTCGCAAACCAAGATTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATAtt                    >  1:575556/1‑141 (MQ=255)                                                                                                                       aaaaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaa                  <  1:215270/141‑1 (MQ=255)                                                                                                                       aaaaTAATGTATATATTTTCGCGAACAAAGCTGATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATAATATTGAAACAATGTTTAAGTCGAAATTTAAAGATATTaa                  <  2:464900/141‑1 (MQ=255)                                                                                                                        aaaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaa                 <  2:979141/141‑1 (MQ=255)                                                                                                                         aaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaaa                >  2:451349/1‑141 (MQ=255)                                                                                                                          aTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaaat               <  1:1029711/141‑2 (MQ=255)                                                                                                                             aaGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCa            >  1:628632/1‑141 (MQ=255)                                                                                                                              aGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCAt           >  1:767072/1‑141 (MQ=255)                                                                                                                              aGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCAt           >  1:569528/1‑141 (MQ=255)                                                                                                                                  tgtatGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTg       >  1:43187/3‑141 (MQ=255)                                                                                                                                  tatatGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTg       >  1:927771/1‑141 (MQ=255)                                                                                                                                      tGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAaga   <  1:283955/141‑1 (MQ=255)                                                                                                                                       ggTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAagag  >  2:930192/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                       TATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAG  >  CP000730/340126‑340400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑