Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 352,096 G→A 5.7% K6K (AAG→AAA)  USA300HOU_0320 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730352,0960GA 5.7% 270.7 / 4.0 87K6K (AAG→AAA) USA300HOU_0320hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (42/40);  new base A (2/3);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

AATTATTATATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGAA  >  CP000730/351958‑352234
                                                                                                                                          |                                                                                                                                           
aaatattatATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAACCGGAAGGAAGTAAACAGATCGATCAATATATTAAAGAGCAAGAGGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCt                                                                                                                                           <  1:1027783/138‑1 (MQ=17)
aaTTATTATATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCt                                                                                                                                           <  2:601877/141‑1 (MQ=255)
aaTTATTATATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCt                                                                                                                                           <  2:878392/141‑1 (MQ=255)
   tattatATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAg                                                                                                                                        <  1:934016/141‑1 (MQ=255)
    attatATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGt                                                                                                                                       >  2:987394/1‑141 (MQ=255)
     ttatATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACATTCGAAGAAAAGCTAAgaa                                                                                                                                      >  2:658604/1‑139 (MQ=255)
      tataTGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCa                                                                                                                                     >  2:601885/1‑141 (MQ=255)
        tatGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCaaa                                                                                                                                   <  2:433835/141‑1 (MQ=255)
          tGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATg                                                                                                                                 >  2:669904/1‑141 (MQ=255)
            tataAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAAGGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAACTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGGNAAATGTa                                                                                                                               >  2:812763/1‑141 (MQ=255)
             ataAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTAc                                                                                                                              <  1:794317/141‑1 (MQ=255)
             ataAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTAc                                                                                                                              <  1:741743/141‑1 (MQ=255)
                aaaaaTTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAAt                                                                                                                           >  2:65925/1‑141 (MQ=255)
                aaaaaTTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAAt                                                                                                                           >  2:1031286/1‑141 (MQ=255)
                  aaaTTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGa                                                                                                                         <  2:508837/141‑1 (MQ=255)
                  aaaTTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGa                                                                                                                         <  1:832468/141‑1 (MQ=255)
                   aaTTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGaa                                                                                                                        <  1:28024/141‑1 (MQ=255)
                     tttGGGGTTATACCAGAAATGGAATACGAAATGGAAGAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGATGAAGCTGAACTATAGGGGCGATAATATGATTTTCGAAGAAAAACTAAGTCAAATGTACAATGAAAt                                                                                                                      >  1:746756/1‑141 (MQ=14)
                       tGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTg                                                                                                                    <  2:285006/141‑1 (MQ=255)
                       tGGGGTTATACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGAGCAATATATTAAAGAGCAAGATGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAACTAAGTCAAATGTACAATGAAATTg                                                                                                                    <  2:90714/141‑1 (MQ=17)
                         gggTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCa                                                                                                                  >  1:476020/1‑141 (MQ=255)
                         gggTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCa                                                                                                                  >  1:155491/1‑141 (MQ=255)
                         gggTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTCCGAAGAAAAGATAAGTCAAATGTACAATGAAATTGCa                                                                                                                  >  2:5057/1‑141 (MQ=255)
                          ggTTTTACCAAAAATGAAATATGAAATGGAAGAAATTAAAGAAATCAAACAATATAATAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGaaaaaaaaa                                                                                                                 <  2:565475/141‑7 (MQ=18)
                            ttttACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAAt                                                                                                               >  2:576673/1‑141 (MQ=255)
                            ttttACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAAt                                                                                                               <  1:310409/141‑1 (MQ=255)
                                aCCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGaaa                                                                                                           <  1:1049277/141‑1 (MQ=255)
                                   aGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCa                                                                                                        <  2:338059/141‑1 (MQ=255)
                                    gAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAg                                                                                                       >  1:106086/1‑141 (MQ=255)
                                      aaTGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGGTGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTg                                                                                                     <  2:951734/141‑1 (MQ=255)
                                      aaTGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTg                                                                                                     >  2:549891/1‑141 (MQ=255)
                                         ggAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGaa                                                                                                  <  2:428764/141‑1 (MQ=255)
                                         ggAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGaa                                                                                                  >  2:583679/1‑141 (MQ=255)
                                             tatGAAATGGAAGAAATTAAAGAAATCGATCAATAGATTAAACAGCAAGATGAAGCTGAAATATAGGTGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGAt                                                                                              >  1:932882/1‑141 (MQ=255)
                                              atGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATa                                                                                             >  1:607819/1‑141 (MQ=255)
                                                 aaaTGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGAGGAGATAACATGACTTTCTAAGAAGAGCTAATTCAAATGTACAATGAATTGGCAAATGAAATCAGTGGAATGATACCa                                                                                          >  1:542046/1‑141 (MQ=18)
                                                       aagaaATTAAAGAAATCGAGCAATATATTAAAGAGCAAGATGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAACTAAGTCAAATGTACAATGAAATTGCAAATAAAATTAGCAGTATGATACCGGTAGaa                                                                                    <  2:786836/141‑1 (MQ=11)
                                                        agaaATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGAAAACATGACTTGCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGTTATGATACCAgaaaaaa                                                                                   <  2:213281/141‑7 (MQ=18)
                                                         gaaATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATg                                                                                  >  2:801303/1‑141 (MQ=255)
                                                         gaaATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATg                                                                                  >  2:128586/1‑141 (MQ=255)
                                                          aaaTTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATgg                                                                                 <  2:694340/141‑1 (MQ=255)
                                                           aaTTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATggg                                                                                <  1:817341/141‑1 (MQ=255)
                                                            aTTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGa                                                                               <  2:157067/141‑1 (MQ=255)
                                                             ttAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGaa                                                                              <  1:405430/141‑1 (MQ=255)
                                                               aaaGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGaaaa                                                                            >  1:1062270/1‑141 (MQ=255)
                                                                aaGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGaaaaa                                                                           >  2:93917/1‑141 (MQ=255)
                                                                aaGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGaaaaa                                                                           >  2:936801/1‑141 (MQ=255)
                                                                 aGAAATCGATCAATATATTAAAGAGCAAGATGAACCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAg                                                                          <  1:1007281/141‑1 (MQ=255)
                                                                    aaTCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGtat                                                                       >  2:487188/1‑141 (MQ=255)
                                                                     aTCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGtata                                                                      <  1:34385/141‑1 (MQ=255)
                                                                      aagATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGAATTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGtatat                                                                     <  2:510237/139‑1 (MQ=255)
                                                                         aTCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACa                                                                  >  1:697322/1‑141 (MQ=255)
                                                                            aaTATATGAAAGAGCAAGCTGAAGCTGAACTATAGGTGCGATAATATTATTTTCGAAGAAAAACTAAGTCAAATGTACAATGATATTGCAAATGAAATCAGCGGGATGATACCAGTAGAGTGGGAAAAAGTATATACAAtt                                                               >  2:676171/1‑141 (MQ=14)
                                                                                  ttAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTAc                                                         >  2:548398/1‑141 (MQ=255)
                                                                                      agagCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGCAGAGAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCGGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAg                                                     >  1:792017/1‑141 (MQ=255)
                                                                                      agagCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAg                                                     >  2:847423/1‑141 (MQ=255)
                                                                                      agagCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAg                                                     <  1:1040329/141‑1 (MQ=255)
                                                                                         gCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAgatg                                                  >  1:661404/1‑141 (MQ=255)
                                                                                            aGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGatgatg                                               <  1:71821/141‑1 (MQ=255)
                                                                                              aTGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGaa                                             >  2:533320/1‑141 (MQ=255)
                                                                                              aTGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGaa                                             >  2:372638/1‑141 (MQ=255)
                                                                                                      gAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGgaga                                     >  2:801390/1‑141 (MQ=255)
                                                                                                        aaTATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAgg                                   <  1:609559/141‑1 (MQ=255)
                                                                                                         atatAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGgt                                  <  2:111506/141‑1 (MQ=255)
                                                                                                            taGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGgtt‑gt                              <  2:155937/141‑1 (MQ=255)
                                                                                                              ggggCGATAATATGATTTTCGAAGAAAAACTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGGATGATACCAGTAGAGTGGGAAAAAGTATATACAATTGCTTATGTAGATGATGAAGGTGGAGAAGTC‑Gttt                            <  2:1060720/141‑1 (MQ=18)
                                                                                                              ggggAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑Gttt                            >  1:134427/1‑141 (MQ=255)
                                                                                                              ggggAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑Gttt                            <  2:840606/141‑1 (MQ=255)
                                                                                                              ggggAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑Gttt                            >  2:435437/1‑141 (MQ=255)
                                                                                                               gggAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑Gtttt                           >  2:410412/1‑141 (MQ=255)
                                                                                                                  agaTAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTaa                        >  1:6312/1‑141 (MQ=255)
                                                                                                                     tAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGG‑GCGTTTGGTTTTTAATta                     <  1:801303/141‑1 (MQ=21)
                                                                                                                      aaCATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATtat                    >  1:747141/1‑141 (MQ=255)
                                                                                                                       aCATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATtata                   <  1:128586/141‑1 (MQ=255)
                                                                                                                         aTGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACt                 >  2:64273/1‑141 (MQ=255)
                                                                                                                          tGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGGGGAGAGGTT‑GTTTTTAATTATACTa                <  2:611151/141‑1 (MQ=255)
                                                                                                                               ttCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCa           <  1:441693/141‑1 (MQ=255)
                                                                                                                                  gaagaaAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGa        >  1:315578/1‑141 (MQ=255)
                                                                                                                                  gaagaaAAGCTAAGTAAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGGAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGa        <  2:1007554/141‑1 (MQ=255)
                                                                                                                                      aaaaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTg    <  2:476020/141‑1 (MQ=255)
                                                                                                                                       aanGCTAAGTCAAATGTACANTGNAATTGCAAATGAAATCAGTGGAATGANACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGa   <  2:520579/141‑1 (MQ=255)
                                                                                                                                       aaaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGa   >  2:61304/1‑141 (MQ=255)
                                                                                                                                       aaaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGa   >  1:315091/1‑141 (MQ=255)
                                                                                                                                       aaaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGa   <  1:576673/141‑1 (MQ=255)
                                                                                                                                       aaaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATAACAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGATGTT‑GTTTTTAATTATACTAAACCAGGAAGTGa   <  1:336664/141‑1 (MQ=255)
                                                                                                                                        aaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGaa  >  2:409782/1‑141 (MQ=255)
                                                                                                                                        aaGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGaa  >  1:727198/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AATTATTATATGTATAAAAAATTTGGGGTTTTACCAGAAATGGAATATGAAATGGAAGAAATTAAAGAAATCGATCAATATATTAAAGAGCAAGATGAAGCTGAAATATAGGGGAGATAACATGACTTTCGAAGAAAAGCTAAGTCAAATGTACAATGAAATTGCAAATGAAATCAGTGGAATGATACCAGTAGAATGGGAAAAAGTATATACAATTGCCTACGTAGATGATGAAGGTGGAGAGGTT‑GTTTTTAATTATACTAAACCAGGAAGTGAA  >  CP000730/351958‑352234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑