Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 355,692 G→T 6.5% V336V (GTC→GTA brnQ1 ← LIVCS family branched chain amino acid:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730355,6920GT 6.5% 347.5 / 6.0 105V336V (GTC→GTAbrnQ1LIVCS family branched chain amino acid:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (52/46);  new base T (5/2);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 107 total aligned reads displayed.

AGTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTAT  >  CP000730/355552‑355832
                                                                                                                                             |                                                                                                                                            
aGTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATggg                                                                                                                                              >  1:990235/1‑141 (MQ=255)
 gTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGa                                                                                                                                             <  2:872493/141‑1 (MQ=255)
 gTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGa                                                                                                                                             <  2:569332/141‑1 (MQ=255)
   aTTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACa                                                                                                                                           <  2:152871/141‑1 (MQ=255)
    tttGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAg                                                                                                                                          >  1:231211/1‑141 (MQ=255)
     ttGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGc                                                                                                                                         <  2:871403/141‑1 (MQ=255)
     ttGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGc                                                                                                                                         >  2:332395/1‑141 (MQ=255)
      tGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCa                                                                                                                                        <  1:157074/141‑1 (MQ=255)
              tGAAATAATCAATGTCATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTaa                                                                                                                                <  1:901425/141‑1 (MQ=255)
                 aataatCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAAt                                                                                                                             >  2:878973/1‑141 (MQ=255)
                 aataatCAATGTAATAACAGTTGCGAATCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAAt                                                                                                                             >  2:720366/1‑141 (MQ=255)
                         aTGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTa                                                                                                                     <  1:820481/141‑1 (MQ=255)
                          tGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAACGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTaa                                                                                                                    >  1:420027/1‑141 (MQ=255)
                          tGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTaa                                                                                                                    >  2:405496/1‑141 (MQ=255)
                             aataaCAGTTGCGAGTCGATAGGCCCGACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAAcc                                                                                                                 <  2:485810/141‑1 (MQ=255)
                                       gCGAGTCGAGAGGCCCAACTGAATCTGAATGTGCGAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTGAAAATCATTTCTAAACCAAGTGTTGTa                                                                                                       >  2:919185/1‑141 (MQ=255)
                                                gactcgcAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATCAGTACAAGTGCAATAGACACGGGATATATTAATGTCAACAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAAc                                                                                              >  2:261558/7‑141 (MQ=255)
                                                    cccAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTa                                                                                          >  1:794655/1‑141 (MQ=255)
                                                     ccAACTGAATCTGAATGTGCGAAACATCTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTGTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTAt                                                                                         <  2:228664/141‑1 (MQ=255)
                                                        aCTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGAt                                                                                      >  2:72150/1‑141 (MQ=255)
                                                          tgaatctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATag                                                                                    >  2:1065161/1‑141 (MQ=255)
                                                            aatctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATagag                                                                                  <  1:379602/141‑1 (MQ=255)
                                                            aatctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATagag                                                                                  >  2:660450/1‑141 (MQ=255)
                                                            aatctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATagag                                                                                  >  2:1060990/1‑141 (MQ=255)
                                                            aatctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAACAACGGGACAGCAATAAATAAAATCATTTCTACACCAAGTGGTGTAAATAAGAACCATATGATagaa                                                                                  >  2:236542/1‑140 (MQ=255)
                                                             atctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAgaga                                                                                 >  2:528439/1‑141 (MQ=255)
                                                               ctgaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGaaa                                                                               <  2:687855/141‑1 (MQ=255)
                                                                 gaatGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAAt                                                                             <  2:710267/141‑1 (MQ=255)
                                                                     gtgCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGt                                                                         <  2:967154/141‑1 (MQ=255)
                                                                      tgCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATTGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTg                                                                        <  2:685171/141‑1 (MQ=255)
                                                                      tgCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTg                                                                        <  1:977092/141‑1 (MQ=255)
                                                                      tgCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTg                                                                        <  1:187407/141‑1 (MQ=255)
                                                                        cTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCg                                                                      >  1:336284/1‑141 (MQ=255)
                                                                         tAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGa                                                                     >  1:1067904/1‑141 (MQ=255)
                                                                         tAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGa                                                                     >  2:385362/1‑141 (MQ=255)
                                                                          aaaCATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGaa                                                                    >  1:651613/1‑141 (MQ=255)
                                                                          aaaCATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGaa                                                                    >  2:79407/1‑141 (MQ=255)
                                                                           aaCATGTTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTAAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGACACCTACGACCGAGCAACAAAGTGCCaa                                                                    >  2:110909/1‑141 (MQ=255)
                                                                               tGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAttt                                                               >  2:669568/1‑141 (MQ=255)
                                                                                gTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAtttt                                                              <  1:743284/141‑1 (MQ=255)
                                                                                gTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAtttt                                                              <  1:383581/141‑1 (MQ=255)
                                                                                   tAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtat                                                            >  1:400932/1‑141 (MQ=255)
                                                                                   tAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtat                                                            <  2:328949/141‑1 (MQ=255)
                                                                                   tAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtat                                                            <  2:686079/141‑1 (MQ=255)
                                                                                   tAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtat                                                            >  2:10020/1‑141 (MQ=255)
                                                                                    aGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtata                                                           >  1:402840/1‑141 (MQ=255)
                                                                                     gCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATaa                                                          >  1:1039951/1‑141 (MQ=255)
                                                                                     gCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATaa                                                          <  1:684463/141‑1 (MQ=255)
                                                                                      cAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAACGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAg                                                         >  1:471969/1‑141 (MQ=255)
                                                                                        aaTGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaa                                                       >  1:369497/1‑141 (MQ=255)
                                                                                          tGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaaaa                                                     >  1:174952/1‑141 (MQ=255)
                                                                                          tGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaaaa                                                     >  2:777196/1‑141 (MQ=255)
                                                                                           gATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaaacc                                                    <  1:452052/141‑3 (MQ=255)
                                                                                           gATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAAc                                                    >  1:429890/1‑141 (MQ=255)
                                                                                            atatGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACt                                                   <  1:551096/141‑1 (MQ=255)
                                                                                              atGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTa                                                 >  2:568226/1‑141 (MQ=255)
                                                                                               tGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCATATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAg                                                >  2:792898/1‑141 (MQ=255)
                                                                                                gAGTTCAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg                                               <  1:513752/141‑1 (MQ=255)
                                                                                                gAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg                                               <  2:550527/141‑1 (MQ=255)
                                                                                                gAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAGTTTATAAGAAAACTTAgg                                               <  2:655398/141‑1 (MQ=255)
                                                                                                 aGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGt                                              >  1:750857/1‑141 (MQ=255)
                                                                                                  gTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTa                                             <  1:166794/141‑1 (MQ=255)
                                                                                                   tACAAGTGCAATCGACACGAGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTAc                                            <  2:904018/141‑1 (MQ=255)
                                                                                                    aCAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACg                                           >  1:1070468/1‑141 (MQ=255)
                                                                                                    aCAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACg                                           <  2:561490/141‑1 (MQ=255)
                                                                                                     cAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgt                                          >  1:988332/1‑141 (MQ=255)
                                                                                                     cAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgt                                          <  2:443845/141‑1 (MQ=255)
                                                                                                     cAAGTGCAATCGACACGGCATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGCACCCTCTGATAGAGAAAATAAGTGCGACTATTTTATACGAAAACTTAGGTACgt                                          <  2:918369/141‑1 (MQ=255)
                                                                                                      aaGTGTAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAATCTTAGGTACgtg                                         <  1:817940/141‑1 (MQ=255)
                                                                                                      aaGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgtg                                         <  1:746782/141‑1 (MQ=255)
                                                                                                      aaGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgtg                                         <  2:334252/141‑1 (MQ=255)
                                                                                                         tGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg                                               >  2:158910/1‑132 (MQ=255)
                                                                                                         tGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg                                               <  1:158910/132‑1 (MQ=255)
                                                                                                             aTCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTa                                  >  1:477268/1‑141 (MQ=255)
                                                                                                               cGACACGGGATATATTAAAGTCAATAATGGGACGGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGt                                >  1:563338/1‑141 (MQ=255)
                                                                                                                  cacGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTaaa                             <  2:509280/141‑1 (MQ=255)
                                                                                                                  cacGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTaaa                             >  1:734716/1‑141 (MQ=255)
                                                                                                                    cGGGATATATTAATGTCAACAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg                           >  2:1016404/1‑141 (MQ=255)
                                                                                                                    cGGGATATATTAATGTCAACAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg                           >  2:474763/1‑141 (MQ=255)
                                                                                                                    cGGGATATATTAAATTTGTTAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg                           <  1:332395/141‑1 (MQ=255)
                                                                                                                    cGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg                           >  1:403016/1‑141 (MQ=255)
                                                                                                                     gggATATATTAAAGTCAATAATGGGACAGCAATTTTTTTTTTTTTTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgt                          >  2:638176/1‑141 (MQ=255)
                                                                                                                     gggATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgt                          >  2:797055/1‑141 (MQ=255)
                                                                                                                      ggATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgtg                         >  1:273157/1‑141 (MQ=255)
                                                                                                                         tatatTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGgcg                      >  2:368046/1‑141 (MQ=255)
                                                                                                                          atatTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGgcgc                     <  2:780795/141‑1 (MQ=255)
                                                                                                                          atatTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGgcgc                     >  1:178336/1‑141 (MQ=255)
                                                                                                                            atTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCaa                   >  1:840078/1‑141 (MQ=255)
                                                                                                                             ttAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAg                  <  1:30320/141‑1 (MQ=255)
                                                                                                                              tAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGc                 >  1:655332/1‑141 (MQ=255)
                                                                                                                                aaGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCAt               <  2:604480/141‑1 (MQ=255)
                                                                                                                                aaGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCAt               <  1:706488/141‑1 (MQ=255)
                                                                                                                                   tCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGa            <  2:1070468/141‑1 (MQ=255)
                                                                                                                                    cAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGAc           <  2:361677/141‑1 (MQ=255)
                                                                                                                                     aataatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACg          <  2:425994/141‑1 (MQ=255)
                                                                                                                                       taatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGt                                                                         >  2:338240/1‑76 (MQ=255)
                                                                                                                                       taatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGt                                                                         <  1:338240/76‑1 (MQ=255)
                                                                                                                                        aatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGt       <  2:1020328/141‑1 (MQ=255)
                                                                                                                                          tGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTcc     >  1:766155/1‑141 (MQ=255)
                                                                                                                                             gACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCtat  <  1:720366/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
AGTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATGTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTAT  >  CP000730/355552‑355832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: