Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,279,545 A→T 9.2% intergenic (+1213/+6819) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,279,5450AT 9.2% 121.6 / 5.1 44intergenic (+1213/+6819)mtlD/glmMmannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base A (21/18);  new base T (2/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TCTTTGCTATCAGCAGATGATGCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCAT  >  CP000730/2279405‑2279681
                                                                                                                                            |                                                                                                                                        
tCTTTGCTATCAGCAGATGATGCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCa                                                                                                                                          <  2:24079/141‑1 (MQ=255)
    tGCTATCCGCAGATGATGCTGCTTCTGTGCTCGCAGTTGTTGGTGCCGTTGCTGTTGATCCTGTTCGTGCATTCTCGTTTGTTGCTGTATTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGttt                                                                                                                                      <  2:12797/141‑1 (MQ=255)
     gCTATCAGCAGATGATGCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTc                                                                                                                                     >  1:364773/1‑141 (MQ=255)
           agcagATGATGCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTat                                                                                                                               >  2:412079/1‑140 (MQ=255)
                  gatgCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTGGTTTGTTGCTGTAGTTGTACTATTGTTCTCTGTTGTGCTTTCTGCTGGCGTTGCATTATCCGTTTCTGTTACTGTTTTa                                                                                                                        >  2:287331/1‑141 (MQ=255)
                  gatgCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTa                                                                                                                        >  1:29510/1‑141 (MQ=255)
                   atgCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTAt                                                                                                                       <  1:247072/141‑1 (MQ=255)
                    tgctgcTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATc                                                                                                                      <  1:248758/141‑1 (MQ=255)
                           tCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTtgtg                                                                                                               <  1:326111/141‑1 (MQ=255)
                                gCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCTGTTTCTGTTACAGGTTTATCAGTTGTGCCGtt                                                                                                          >  1:110940/1‑141 (MQ=255)
                                   cGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGttatt                                                                                                       <  2:62285/141‑1 (MQ=255)
                                   cGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGttatt                                                                                                       <  1:143062/141‑1 (MQ=255)
                                    gCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGttatta                                                                                                      >  2:187313/1‑141 (MQ=255)
                                     cAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTTTTACAGGTTTATCAGTTGTGCCGTTATTAg                                                                                                     >  2:48816/1‑141 (MQ=255)
                                     cAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAg                                                                                                     >  2:226136/1‑141 (MQ=255)
                                       gttgttGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGt                                                                                                                           <  1:452639/117‑1 (MQ=255)
                                       gttgttGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGt                                                                                                                           >  2:452639/1‑117 (MQ=255)
                                          gttgGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGAt                                                                                                <  1:312315/141‑1 (MQ=255)
                                                       cTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGtt                                                                                   >  1:12963/1‑141 (MQ=255)
                                                        tGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGttt                                                                                  >  1:37846/1‑141 (MQ=255)
                                                               ccTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGtt                                                                           <  2:372272/141‑1 (MQ=255)
                                                                        gCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTCCATCGTTa                                                                  >  1:291397/1‑141 (MQ=255)
                                                                           ttCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTGGTTGTGGCTTCTACTGTCGTTGCATTATCATTTTCTGTTACATTTTTATCAGTTCTGCTGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATcc                                                               <  2:327813/141‑1 (MQ=255)
                                                                                   tgttgCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCgg                                                       <  1:338154/141‑1 (MQ=255)
                                                                                      tgCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCTGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACt                                                    <  1:312308/141‑1 (MQ=255)
                                                                                      tgCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTATTTACATCGTTATCCATTGTCGGACt                                                    >  2:283376/1‑141 (MQ=255)
                                                                                      tgCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACt                                                    <  1:34046/141‑1 (MQ=255)
                                                                                      tgCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACt                                                    <  1:261157/141‑1 (MQ=255)
                                                                                       gCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtg                                                   >  1:103411/1‑141 (MQ=255)
                                                                                       gCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtg                                                   >  2:253845/1‑141 (MQ=255)
                                                                                        cTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtgt                                                  <  1:395763/141‑1 (MQ=255)
                                                                                         tGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtgtt                                                 >  1:186525/1‑141 (MQ=255)
                                                                                         tGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtgtt                                                 >  1:186591/1‑141 (MQ=255)
                                                                                          gTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtgttt                                                >  1:257929/1‑141 (MQ=255)
                                                                                           tAGTTNTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACtgtttg                                               <  1:147960/141‑1 (MQ=255)
                                                                                                               gttgtGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGaa                           <  1:215200/141‑1 (MQ=255)
                                                                                                                       ttCTGCTGGCGTTGCATTATCTGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTGCACTAGAATTGttatt                   <  2:110940/141‑1 (MQ=255)
                                                                                                                        tCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGttatta                  >  2:246370/1‑141 (MQ=255)
                                                                                                                        tCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGttatta                  >  1:448231/1‑141 (MQ=255)
                                                                                                                         ctgctgGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAg                 >  2:1564/1‑141 (MQ=255)
                                                                                                                              ggCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGc            >  1:372388/1‑141 (MQ=255)
                                                                                                                                cGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCgg          >  1:231323/1‑141 (MQ=255)
                                                                                                                                cGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCgg          <  2:396932/141‑1 (MQ=255)
                                                                                                                                     cATTATCTGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTGCACTAGAATTGTTATTAGCTTGCGGTTTAt     >  1:282660/1‑141 (MQ=255)
                                                                                                                                        tATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCAt  <  1:214234/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TCTTTGCTATCAGCAGATGATGCTGCTTCTGTGCTCGCAGTTGTTGGAGCCGTTGCTGTTGATCCTGTTGGTGCATTCTCGTTTGTTGCTGTAGTTGTACTATTGTTATTTGTTGTGCTTTCTGCTGGCGTTGCATTATCAGTTTCTGTTACAGGTTTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCAT  >  CP000730/2279405‑2279681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑