Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,340,488 T→A 8.2% G751G (GGT→GGA)  hysA → hyaluronate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,340,4880TA 8.2% 143.4 / 5.2 49G751G (GGT→GGA) hysAhyaluronate lyase
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base A (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GATGAAGTAACTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTCG  >  CP000730/2340348‑2340623
                                                                                                                                            |                                                                                                                                       
gATGAAGTAACTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATACTGAATCGGTTTGAGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGt                                                                                                                                         >  1:214857/1‑141 (MQ=255)
    aaGTAACTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGt                                                                                                                                     <  2:63468/141‑1 (MQ=255)
        aaCTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTtat                                                                                                                                 >  1:336018/1‑141 (MQ=255)
         aCTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTtata                                                                                                                                >  1:318464/1‑141 (MQ=255)
         aCTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTtata                                                                                                                                <  1:408647/141‑1 (MQ=255)
           tGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACt                                                                                                                              >  1:177291/1‑141 (MQ=255)
           tGTCGTTAAACAAGATGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGAATGtttcttct                                                                                                                              >  1:272/1‑136 (MQ=255)
            gTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACtt                                                                                                                             <  1:288534/141‑1 (MQ=255)
              cGTTAAGAAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATAATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTaa                                                                                                                           <  2:182405/141‑1 (MQ=255)
               gTTAAACAAGATGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGAATGTTTATTCTTaaa                                                                                                                          <  1:339500/141‑1 (MQ=255)
                    gCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAaaagaa                                                                                                                     >  1:26131/1‑141 (MQ=255)
                     cAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAaaagaaa                                                                                                                    >  1:191916/1‑141 (MQ=255)
                                        ttGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAAt                                                                                                 <  2:266280/141‑1 (MQ=255)
                                          gtgAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGt                                                                                               >  1:386055/1‑141 (MQ=255)
                                          gtgAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGt                                                                                               >  2:208381/1‑141 (MQ=255)
                                          gtgAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGt                                                                                               >  2:208376/1‑141 (MQ=255)
                                          gtgAAAGATAATGAATCGGTCTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGAATGTTTATTCTTAAAAACAAAGATGATAACACTTATGAATGt                                                                                               >  1:39053/1‑141 (MQ=255)
                                                             tttGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAAt                                                                            >  1:120488/1‑141 (MQ=255)
                                                                gggCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGAATGTTTATTCTTAAAAACAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCCGAATCTa                                                                         <  1:350201/141‑1 (MQ=255)
                                                                      gtgtTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAAtt                                                                   <  2:71564/141‑1 (MQ=255)
                                                                             ttATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTc                                                            <  1:318294/141‑1 (MQ=255)
                                                                              tataGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCa                                                           >  1:176455/1‑141 (MQ=255)
                                                                                      tAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGa                                                   >  1:279185/1‑141 (MQ=255)
                                                                                       aGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGaa                                                  >  2:404716/1‑141 (MQ=255)
                                                                                       aGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGaa                                                  >  2:323741/1‑141 (MQ=255)
                                                                                            tCAAACTTTTGACATTAACAACACTAAAGTAGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTaa                                             <  2:308277/141‑1 (MQ=255)
                                                                                               aaCTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAAt                                          <  1:293720/141‑1 (MQ=255)
                                                                                                   tttGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCa                                      <  1:441896/141‑1 (MQ=255)
                                                                                                     tGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTGTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAAt                                    >  1:215163/1‑141 (MQ=255)
                                                                                                         aTTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACGAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGAcc                                <  2:46628/141‑1 (MQ=255)
                                                                                                         aTTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGAcc                                <  2:176035/141‑1 (MQ=255)
                                                                                                           tAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCgg                              <  2:211091/141‑1 (MQ=255)
                                                                                                            aacaacACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGt                             <  2:416435/141‑1 (MQ=255)
                                                                                                            aacaacACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGt                             <  1:291096/141‑1 (MQ=255)
                                                                                                               aacaCTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGtt                            <  2:170285/139‑1 (MQ=255)
                                                                                                               aacaCTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGtt                            >  1:170285/1‑139 (MQ=255)
                                                                                                                 cacTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTActc                        >  2:161234/1‑141 (MQ=255)
                                                                                                                 cacTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTActc                        <  2:11230/141‑1 (MQ=255)
                                                                                                                  acTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTActct                       >  2:118174/1‑141 (MQ=255)
                                                                                                                   cTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTa                      >  2:216273/1‑141 (MQ=255)
                                                                                                                           gAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTacaaac              >  1:314753/1‑141 (MQ=255)
                                                                                                                            aaGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTacaaaca             <  2:115542/141‑1 (MQ=255)
                                                                                                                             aGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTacaaacaa            >  1:168417/1‑141 (MQ=255)
                                                                                                                                tAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACTCTTATGTATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCATATATTGAATCTACAATTTCAATGACGGGTTACTCTATTACAAACaaaaa         <  2:191916/141‑1 (MQ=255)
                                                                                                                                tAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACaaaaa         <  2:69886/141‑1 (MQ=255)
                                                                                                                                  aaGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATa       >  2:48047/1‑141 (MQ=255)
                                                                                                                                  aaGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATa       >  2:48053/1‑141 (MQ=255)
                                                                                                                                      cAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTc   <  2:150568/141‑1 (MQ=255)
                                                                                                                                      cAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAAGTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTc   <  2:214857/141‑1 (MQ=255)
                                                                                                                                       aaaGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTCg  >  2:352103/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GATGAAGTAACTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAACACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACTTATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAAATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTCG  >  CP000730/2340348‑2340623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑