| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 284,902 | T→C | 13.4% | A241A (GCA→GCG) | ptsG ← | PTS family glucose/glucoside (glc) porter component IIABC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 284,902 | 0 | T | C | 13.4% | 62.0 / 4.6 | 30 | A241A (GCA→GCG) | ptsG | PTS family glucose/glucoside (glc) porter component IIABC |
| Reads supporting (aligned to +/- strand): ref base T (15/11); new base C (2/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
CGTAATAAAACGGCCTGACATAAAGCGTGACACACCTGAATAATACTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACATGATAAATGCCGGCT > CP000730/284767‑285037 | cGTAATAAAACGGCCTGACATAAAGCGTGACACACCTGAATAATACTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGt < 2:166526/141‑1 (MQ=255) ataaAACTGCGTGAGATAAAGGGTGACACACCTGAAAAATACTTCGTCACATCTGGATCACAAAGTTGATCAAAGAAAATGTTCTGCGTACCTTGAACTAATTGCCCTTGTACTTCTAAAGTACTCCCAATTGCCGTCTgt < 2:238400/141‑2 (MQ=255) aaCGGCCTGACATAAAGCGTGACACAACTGCATAATACTTCGTCACATCTGGATCACCAATTTGAGCAAAGAAGATGTTCTGCGTACCTTAAACTAAGTGCCCTTTGACTTCTAAAATACCACCAAGTGCCGTCTGCCaaa > 2:42134/1‑141 (MQ=255) acacCTGAATAATACTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGgtgt < 2:187301/141‑1 (MQ=255) ataCTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTgg > 1:218175/1‑141 (MQ=255) ataCTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTgg > 1:697/1‑141 (MQ=255) aCTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGCGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGAc > 1:113968/1‑141 (MQ=255) aCTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGCGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGAc > 1:114237/1‑141 (MQ=255) cGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTaa > 1:132257/1‑141 (MQ=255) tCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACa > 2:453552/1‑141 (MQ=255) gATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGa > 2:235518/1‑141 (MQ=255) gATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGa > 2:336468/1‑141 (MQ=255) cACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGa > 2:451552/1‑141 (MQ=255) aGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCa < 1:308574/141‑1 (MQ=255) cAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACaa > 2:17915/1‑141 (MQ=255) agaagaTGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAg > 1:75045/1‑141 (MQ=255) gTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCACAAACAAAAGTACCGAt > 2:35666/1‑141 (MQ=255) ttCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATg > 2:374603/1‑141 (MQ=255) cTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGc < 2:211010/141‑1 (MQ=255) tGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCa < 1:248811/141‑1 (MQ=255) cGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCAcc < 2:77252/141‑1 (MQ=255) aCCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGt < 1:200131/141‑1 (MQ=255) cTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGttt < 2:426242/141‑1 (MQ=255) gACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAAc > 1:31503/1‑141 (MQ=255) aCTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACa > 1:305724/1‑141 (MQ=255) accaccAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACATGATAAATGcc > 1:318627/1‑141 (MQ=255) caccaAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACACGATAAATGCCgg < 2:159400/141‑1 (MQ=255) ccaAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACATGATAAATGCCGGCt < 2:301281/141‑1 (MQ=255) ccaAGCGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACATGATAAATGCCGGCt < 2:114237/141‑1 (MQ=255) ccaAGCGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACATGATAAATGCCGGCt < 2:113968/141‑1 (MQ=255) | CGTAATAAAACGGCCTGACATAAAGCGTGACACACCTGAATAATACTTCGTCACATCTGGATCACCAAGTTGAGCAAAGAAGATGTTCTGCGTACCTTGAACTAAGTGCCCTTTGACTTCTAAAGTACCACCAAGTGCCGTCTGCCAAAACGGTAAGTAAAAAATATGGTGTAAACCGAGTGGACCTAACAATCTTAAGATGAAGCCATAAACAAAAGTACCGATGGCACCTGTTTTCGTTACAAATCCACCAACATGATAAATGCCGGCT > CP000730/284767‑285037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |