| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,451,876 | T→C | 12.5% | A682A (GCA→GCG) | sucA ← | oxoglutarate dehydrogenase (succinyl‑transferring) E1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,451,876 | 0 | T | C | 12.5% | 93.7 / 6.6 | 40 | A682A (GCA→GCG) | sucA | oxoglutarate dehydrogenase (succinyl‑transferring) E1 |
| Reads supporting (aligned to +/- strand): ref base T (14/21); new base C (3/2); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01 | |||||||||||
TGAATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGT > CP000730/1451738‑1452010 | tgaATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGc < 1:319374/141‑1 (MQ=255) ttCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCa > 1:394980/1‑141 (MQ=255) tCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCaa > 2:220653/1‑141 (MQ=255) tCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCaa > 2:66321/1‑141 (MQ=255) cccTCATATGCAAGAGGTAAGAATAATGTTAATCCTGTACGTTCTCCCGTTTTTGAGCGAGAACTGAATAAGAAGTTGTTAAAAAAAATTTGTGACATATTTGCAAAATCACCATATTGTGTGTTCAAAATATTGAAGCtt > 2:100635/1‑141 (MQ=255) cTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACTTATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCtttt < 1:371274/141‑1 (MQ=255) cTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCtttt < 1:6864/141‑1 (MQ=255) tCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCttttt > 2:87245/1‑141 (MQ=255) ttatGCATGATGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCttttttt < 2:371784/140‑1 (MQ=255) tatGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTg < 1:144227/141‑1 (MQ=255) gCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGttt > 1:124855/1‑141 (MQ=255) cATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGCGATTCCCAAATATTGAAGCTTTTTTTGtttt > 2:421241/1‑141 (MQ=255) aTGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTc < 2:162935/141‑1 (MQ=255) tGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTcc < 1:450079/141‑1 (MQ=255) aGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCcac < 2:390487/141‑1 (MQ=255) aGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATtat > 1:266388/1‑141 (MQ=255) gTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTAt > 2:203176/1‑141 (MQ=255) aaTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCa > 1:399332/1‑141 (MQ=255) aTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCaa > 2:323983/1‑141 (MQ=255) aTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCaa > 2:324321/1‑141 (MQ=255) gAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCa < 2:90939/141‑1 (MQ=255) tttGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCt < 1:160859/141‑1 (MQ=255) gagaACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCg < 2:124855/141‑1 (MQ=255) aCTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGgaga > 1:439321/1‑141 (MQ=255) gAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAAtt < 2:73597/141‑1 (MQ=255) tAAGAAGTTGTCAAAAATCATTCGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATtgtg < 1:379727/141‑1 (MQ=255) agaagTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGta < 1:74055/141‑1 (MQ=255) tGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCaa > 1:198625/1‑141 (MQ=255) cAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATg < 1:310588/141‑1 (MQ=255) tgtgACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGa < 1:323983/141‑1 (MQ=255) tgtgACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGa < 1:324321/141‑1 (MQ=255) gACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCa < 1:407914/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 1:207827/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 2:75852/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 1:64851/141‑1 (MQ=255) tGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACatg > 1:6676/1‑141 (MQ=255) aaaaTCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACatgatg < 1:217612/141‑1 (MQ=255) aCCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAgg < 2:349005/141‑1 (MQ=255) ccATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGgt > 1:95076/1‑141 (MQ=255) atatTGTGCTTCCCAAATATTGAAGCTTTTTTTTTTTTTTTTTTTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCCAATGTCGCTTTTTGATCAGTAACATGATGTAAATgttt > 2:274404/1‑139 (MQ=255) | TGAATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGT > CP000730/1451738‑1452010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |