Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,880,650 A→T 12.7% I1523I (ATT→ATA fmtB1 ← cell surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,880,6500AT12.7% 131.9 / 9.9 47I1523I (ATT→ATAfmtB1cell surface anchored protein
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base T (3/3);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

TTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTAT  >  CP000730/1880519‑1880789
                                                                                                                                   |                                                                                                                                           
tttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCgcgc                                                                                                                                    >  1:77994/1‑141 (MQ=255)
  tGTTTCAACACGTTTCACATCATTATTCGTTACTGCTTGATTGATGTTTTGTAATGCAAGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTcttgctt                                                                                                                                  <  2:319218/141‑7 (MQ=255)
  tGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTGGCTCAATTTCGCGCtt                                                                                                                                  >  1:298063/1‑141 (MQ=255)
        aaCACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCATTTTCGCGCTTTTTAGc                                                                                                                            >  2:221913/1‑141 (MQ=255)
                 cacaTCATGATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttt                                                                                                                   >  2:11291/1‑140 (MQ=255)
                  acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                  <  1:248864/141‑1 (MQ=255)
                           ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                                 <  2:142168/133‑1 (MQ=255)
                           ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                                 >  1:142168/1‑133 (MQ=255)
                             cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                                       >  2:124267/1‑141 (MQ=255)
                               cTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAt                                                                                                     >  1:365137/1‑141 (MQ=255)
                               cTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAt                                                                                                     <  2:442547/141‑1 (MQ=255)
                                       tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                             >  2:137927/1‑141 (MQ=255)
                                       tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                             >  1:317975/1‑141 (MQ=255)
                                            gATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgc                                                                                        <  1:322999/141‑1 (MQ=255)
                                             aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                       <  2:449292/141‑1 (MQ=255)
                                             aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                       <  2:449295/141‑1 (MQ=255)
                                              tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                      <  2:77994/141‑1 (MQ=255)
                                              tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                      >  1:97959/1‑141 (MQ=255)
                                                      aaTGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAata                                                                              >  1:174575/1‑141 (MQ=255)
                                                         gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                           >  1:197752/1‑141 (MQ=255)
                                                           aCGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTc                                                                         <  2:2737/141‑1 (MQ=255)
                                                                  tCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTc                                                                  >  1:250195/1‑141 (MQ=255)
                                                                    attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                >  2:302775/1‑137 (MQ=255)
                                                                    attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                >  2:417665/1‑137 (MQ=255)
                                                                       tttttttAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCTCGCTTTTTAGCTTTATATTGTTTCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCTGGCGTCACTaa                                                             >  2:307760/7‑141 (MQ=255)
                                                                        ttCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTaaa                                                            <  1:264628/141‑1 (MQ=255)
                                                                            cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                        <  1:137927/141‑1 (MQ=255)
                                                                            cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                        <  1:169884/141‑1 (MQ=255)
                                                                                  gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                                  <  1:341344/141‑1 (MQ=255)
                                                                                  gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                                  <  2:364864/141‑1 (MQ=255)
                                                                                    tttAAAGCAACATGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCa                                                <  2:278078/141‑1 (MQ=255)
                                                                                        aaGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAgcg                                            <  1:48522/141‑1 (MQ=255)
                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                       >  2:397000/1‑141 (MQ=255)
                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                       >  1:152818/1‑141 (MQ=255)
                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                       >  1:418040/1‑141 (MQ=255)
                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                       >  2:363787/1‑141 (MQ=255)
                                                                                              cTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTATCAAGCGCTTGAc                                      >  1:429216/1‑141 (MQ=255)
                                                                                                tGTTTTTCTTCATCAGGCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACtt                                    >  2:204606/1‑141 (MQ=255)
                                                                                                                   cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                 <  2:362767/141‑1 (MQ=255)
                                                                                                                   cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                 <  1:399199/141‑1 (MQ=255)
                                                                                                                   cATGTTCCGCTCGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                 <  1:365245/141‑1 (MQ=255)
                                                                                                                    aTGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgttg                <  1:339573/141‑1 (MQ=255)
                                                                                                                     tGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACACTATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGa               >  1:335211/1‑141 (MQ=255)
                                                                                                                           gCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGtt         <  2:328526/141‑1 (MQ=255)
                                                                                                                               gCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtat     >  1:391697/1‑141 (MQ=255)
                                                                                                                                  cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat  <  1:162647/141‑1 (MQ=255)
                                                                                                                                  cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat  >  1:26570/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                           
TTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTAT  >  CP000730/1880519‑1880789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: