Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,373,019	G→A	Q253* (CAG→TAG)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,373,019	0	G	A	100.0%	99.0 / NA	34	Q253* (CAG→TAG)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/23);  total (11/23)

AATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTGATG  >  CP000730/2372881‑2373155                                                                                                                                           |                                                                                                                                         aagcTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACtata                                                                                                                                        <  2:124536/138‑1 (MQ=255)   tCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATc                                                                                                                                      <  1:329306/141‑1 (MQ=255)    cTGAGAACAATGCTCTACCAGCTCTTGGTACTTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGACGTCAATAAATAAGAACGTTAAAGTAGCAATTAAGAGCTATATCg                                                                                                                                     <  2:277137/141‑1 (MQ=255)    cTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCg                                                                                                                                     <  1:132313/141‑1 (MQ=255)    cTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCg                                                                                                                                     <  2:403948/141‑1 (MQ=255)    cTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCg                                                                                                                                     <  1:30826/141‑1 (MQ=255)    cTGAGAACAATGCACTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCg                                                                                                                                     <  2:82899/141‑1 (MQ=255)          aCAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTtaata                                                                                                                               >  1:221170/1‑141 (MQ=255)           cAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTtaataa                                                                                                                              <  2:318121/141‑1 (MQ=255)           cAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCGACGATTGTACCAGCTGGATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTtaataa                                                                                                                              >  1:289926/1‑141 (MQ=255)                    aCCAGCTCTTTGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAANAATTAAGAACTATATCGTTAATAATTGACTTgg                                                                                                                     <  1:332423/141‑1 (MQ=255)                    aCCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAGCAATTAAGAACTATATCGTTAATAATTGACTTgg                                                                                                                     <  2:143348/141‑1 (MQ=255)                    aCCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTgg                                                                                                                     <  2:204794/141‑1 (MQ=255)                      cAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGt                                                                                                                   <  1:2027/141‑1 (MQ=255)                           ctTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTa                                                                                                              >  2:355873/1‑141 (MQ=255)                               gTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACg                                                                                                          >  1:216368/1‑141 (MQ=255)                                     tattatCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCaa                                                                                                    <  2:359746/141‑1 (MQ=255)                                     tattatCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCaa                                                                                                    <  1:4609/141‑1 (MQ=255)                                     tattatCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCaa                                                                                                    <  2:68556/141‑1 (MQ=255)                                     tattatCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCaa                                                                                                    <  1:4677/141‑1 (MQ=255)                                                    taCCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACg                                                                                     <  1:57202/141‑1 (MQ=255)                                                     aCCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGt                                                                                    >  1:346605/1‑141 (MQ=255)                                                                 aGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACt                                                                        <  2:175290/141‑1 (MQ=255)                                                                        gCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATc                                                                 <  2:182460/141‑1 (MQ=255)                                                                        gCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATc                                                                 <  2:253178/141‑1 (MQ=255)                                                                                        cTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATNTTACCCACAATACCAg                                                 <  1:355873/141‑1 (MQ=255)                                                                                                      tCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGaatc                                   >  2:248105/1‑141 (MQ=255)                                                                                                      tCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGaatc                                   >  1:71197/1‑141 (MQ=255)                                                                                                      tCAATAAATAAGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGaatc                                   >  2:160260/1‑141 (MQ=255)                                                                                                         ataaataaGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGaatcaat                                >  1:130594/1‑141 (MQ=255)                                                                                                         ataaataaGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGaatcaat                                >  1:14710/1‑141 (MQ=255)                                                                                                         ataaataaGAACGTTAAAATAACAATTAAGAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGaatcaat                                >  1:183290/1‑141 (MQ=255)                                                                                                                                      gAACTATATCGTTAATACTTGACTTGTGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGtgat   <  2:289926/141‑1 (MQ=255)                                                                                                                                      gAACTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGtgat   <  2:216368/141‑1 (MQ=255)                                                                                                                                       aaCTATATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGCTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGtgatg  <  2:31404/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         AATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATACTTGGAATCTTACCCACAATACCAGATGGCATTTGAATCAATCCAGTGAACATACCAACGATTGCTGTGATG  >  CP000730/2372881‑2373155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑